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The map label for this gene is ampD [H]

Identifier: 77457014

GI number: 77457014

Start: 924598

End: 925158

Strand: Direct

Name: ampD [H]

Synonym: Pfl01_0787

Alternate gene names: 77457014

Gene position: 924598-925158 (Clockwise)

Preceding gene: 77457013

Following gene: 77457015

Centisome position: 14.36

GC content: 61.5

Gene sequence:

>561_bases
ATGCAGTTGGATCCCGCAAGCGGGTGGTGTCACGGGGTGCAGGTCTGCCCTTCGCCCAACTTCAATGAACGCCCGGCGGG
CGAAGTCTCCCTGTTGGTCATCCACAACATCAGCTTGCCACCGGCGCAGTTCGCCACCGGCAAGGTCCAGGAATTTTTCC
AGAATCGTCTGGATGTCACGGAACATCCCTACTTTGCCGGGATCGCCGACCTGCGCGTGTCTGCGCATTTTCTGATCGAA
CGTGACGGCAAGGTCACCCAGTTTGTCTCCTGTCTTGAGCGTGCCTGGCATGCCGGTGTTTCGGTGTTCGAAGGACGGGA
AACCTGTAACGATTTTTCCGTGGGCATCGAGCTGGAAGGCACCGATGATCTGCCGTTCACCGATGCGCAGTACCAGGCGT
TGACCGTGCTGACCCGGCAGATACAGGCGGCGTTCCCGGCGATCACCGTCGAGCGCATCTGCGGGCACAGCGACATCGCC
CCCGGACGCAAGACCGACCCCGGACCCGCATTCGACTGGGCACGCTACCGCGCCGCCCTGGCACAAGAGGAAGGACAATG
A

Upstream 100 bases:

>100_bases
CTCGGCAATCTGCGGCGGCTCAATCGCGGCAAGTGATCGCGCGCCGGGGGCCGATCACGGCATACTGGTCGCCAACACAG
TCGGCGTTGAAGGAATCGGT

Downstream 100 bases:

>100_bases
GTTTTCTGGTGTTACTGCTGGCGGTCTGGATCGAGAAGTTCTCGGCTCTGCGCCAACGGGTGCAACGCGATGGCGGATGG
ATCCGCGAACTGCACAAGCT

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MQLDPASGWCHGVQVCPSPNFNERPAGEVSLLVIHNISLPPAQFATGKVQEFFQNRLDVTEHPYFAGIADLRVSAHFLIE
RDGKVTQFVSCLERAWHAGVSVFEGRETCNDFSVGIELEGTDDLPFTDAQYQALTVLTRQIQAAFPAITVERICGHSDIA
PGRKTDPGPAFDWARYRAALAQEEGQ

Sequences:

>Translated_186_residues
MQLDPASGWCHGVQVCPSPNFNERPAGEVSLLVIHNISLPPAQFATGKVQEFFQNRLDVTEHPYFAGIADLRVSAHFLIE
RDGKVTQFVSCLERAWHAGVSVFEGRETCNDFSVGIELEGTDDLPFTDAQYQALTVLTRQIQAAFPAITVERICGHSDIA
PGRKTDPGPAFDWARYRAALAQEEGQ
>Mature_186_residues
MQLDPASGWCHGVQVCPSPNFNERPAGEVSLLVIHNISLPPAQFATGKVQEFFQNRLDVTEHPYFAGIADLRVSAHFLIE
RDGKVTQFVSCLERAWHAGVSVFEGRETCNDFSVGIELEGTDDLPFTDAQYQALTVLTRQIQAAFPAITVERICGHSDIA
PGRKTDPGPAFDWARYRAALAQEEGQ

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=177, Percent_Identity=55.3672316384181, Blast_Score=202, Evalue=1e-53,
Organism=Escherichia coli, GI1787092, Length=125, Percent_Identity=41.6, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 20515; Mature: 20515

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLDPASGWCHGVQVCPSPNFNERPAGEVSLLVIHNISLPPAQFATGKVQEFFQNRLDVT
CCCCCCCCCCCCEEECCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCC
EHPYFAGIADLRVSAHFLIERDGKVTQFVSCLERAWHAGVSVFEGRETCNDFSVGIELEG
CCCCHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEECC
TDDLPFTDAQYQALTVLTRQIQAAFPAITVERICGHSDIAPGRKTDPGPAFDWARYRAAL
CCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHH
AQEEGQ
HHCCCC
>Mature Secondary Structure
MQLDPASGWCHGVQVCPSPNFNERPAGEVSLLVIHNISLPPAQFATGKVQEFFQNRLDVT
CCCCCCCCCCCCEEECCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCC
EHPYFAGIADLRVSAHFLIERDGKVTQFVSCLERAWHAGVSVFEGRETCNDFSVGIELEG
CCCCHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEECC
TDDLPFTDAQYQALTVLTRQIQAAFPAITVERICGHSDIAPGRKTDPGPAFDWARYRAAL
CCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHH
AQEEGQ
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]