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The map label for this gene is nusA [H]

Identifier: 77457005

GI number: 77457005

Start: 912231

End: 913712

Strand: Direct

Name: nusA [H]

Synonym: Pfl01_0778

Alternate gene names: 77457005

Gene position: 912231-913712 (Clockwise)

Preceding gene: 77457004

Following gene: 77457006

Centisome position: 14.17

GC content: 57.69

Gene sequence:

>1482_bases
ATGAGCAAAGAAGTACTGCTGGTTGTTGAGTCGGTATCCAATGAAAAGGGTGTACCGGCAAGCGTAATTTTTGAAGCGCT
GGAGCTGGCTCTGGCCACTGCTACCAAAAAGCGTTTTGAAGACGAAGTTGATCTGCGTGTGGAAATCAACCGCCACACCG
GTTCCTACGAAACATTCCGTCGCTGGACGGTCGTCGAAGAGAATGATCTCGATGATCCGGCCATCGAAACCTGGCCGAGC
AAGGTTGCTGAAACGCACCCTGGCGCCAAGGTTGGCGACGTCGTTGAAGAAAAGATCGAGTCCATCGAGTTCGGCCGCAT
CGCTGCGCAGACCGCCAAGCAGGTCATCGTGCAGAAAGTGCGCGAAGCCGAACGCGCTCAAGTGGTTGACGCTTATCGCG
AGCGCCTCGGAGAAATCATCTCCGGCACCGTGAAGAAAGTGACCCGCGACAACGTGATCGTCGACTTGGGTAATAACGCT
GAAGCGTTGCTGGCCCGTGAAGACATCATTTCTCGCGAAACCTTCCGTGTTGGCGTGCGTCTGCGTGCGCTGCTCAAGGA
AATCCGCACCGAGAACCGCGGTCCTCAGCTGATCCTGTCGCGTACCGCGCCGGAAATGCTGATCGAGCTGTTCCGTATCG
AAGTGCCGGAAATTGCTGAAGGTCTGATCGAAGTCATGGCCGCATCCCGTGATCCGGGTTCGCGTGCCAAGATCGCTGTC
CGTTCCAAGGACAAACGCATCGACCCGCAAGGCGCGTGCATCGGTATGCGCGGTTCGCGCGTCCAGGCCGTGTCGGGCGA
GTTGGGTGGCGAGCGTGTCGACATCGTCCTTTGGGACGATAATCCGGCTCAGTTCGTGATCAATGCCATGTCGCCGGCAG
AAGTGGCGGCCATCATCGTTGACGAAGATGCCCATGCCATGGACATCGCCGTTGGCGCAGACAATCTGGCTCAGGCCATC
GGTCGTGGTGGTCAGAACGTGCGTCTGGCGAGCCAGCTGACTGGCTGGACCCTGAACGTGATGACCGAATCGGACATCCA
GGCTAAGCAGCAAGCGGAAACCGGCGACATCCTGCGCAACTTCATCGACGAGCTGGAAGTCGACGAAGAACTGGCTCAGG
TGCTGGTAGACGAAGGCTTCACCAGCCTGGAAGAGATTGCCTACGTACCGTTGGAAGAAATGCTCAACATCGACGGCTTT
GACGAAGACATCGTCAACGAGCTTCGCGCTCGTGCCAAGGATCGCTTGTTGACCAAAGCCATCGCTACTGAGGAAAAGCT
GGCAGACGCCCATCCGGCCGAAGACCTGCTCTCGCTTGAGGGTATGGACAAGGATTTGGCGATGGAACTGGCGGTGCGCG
GCGTAATTACCCGCGAAGACCTGGCCGAGCAGTCTATTGACGACCTGCTCGACATCGACGGCATTGACGATGATCGTGCC
GGCAAGTTGATCATGGCCGCCCGAGCCCACTGGTTCGAGTAA

Upstream 100 bases:

>100_bases
TGTTGCCGATCGATATGATCGACAAGGCCAACATTATTCCCAGTTTTGACTGAGACGCGGATCCCGCGGATCCAATGGCT
TGCGAAAGGCGAGGCGTACG

Downstream 100 bases:

>100_bases
TTAGGCGCGGCCTGAGGAGAGAAGTGCATGACGCAAGTCACGGTGAAACAACTGGCCGATGAGGTCAAAACACCGGTAGA
GCGCCTGTTGCAGCAGATGC

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: L factor; N utilization substance protein A [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSKEVLLVVESVSNEKGVPASVIFEALELALATATKKRFEDEVDLRVEINRHTGSYETFRRWTVVEENDLDDPAIETWPS
KVAETHPGAKVGDVVEEKIESIEFGRIAAQTAKQVIVQKVREAERAQVVDAYRERLGEIISGTVKKVTRDNVIVDLGNNA
EALLAREDIISRETFRVGVRLRALLKEIRTENRGPQLILSRTAPEMLIELFRIEVPEIAEGLIEVMAASRDPGSRAKIAV
RSKDKRIDPQGACIGMRGSRVQAVSGELGGERVDIVLWDDNPAQFVINAMSPAEVAAIIVDEDAHAMDIAVGADNLAQAI
GRGGQNVRLASQLTGWTLNVMTESDIQAKQQAETGDILRNFIDELEVDEELAQVLVDEGFTSLEEIAYVPLEEMLNIDGF
DEDIVNELRARAKDRLLTKAIATEEKLADAHPAEDLLSLEGMDKDLAMELAVRGVITREDLAEQSIDDLLDIDGIDDDRA
GKLIMAARAHWFE

Sequences:

>Translated_493_residues
MSKEVLLVVESVSNEKGVPASVIFEALELALATATKKRFEDEVDLRVEINRHTGSYETFRRWTVVEENDLDDPAIETWPS
KVAETHPGAKVGDVVEEKIESIEFGRIAAQTAKQVIVQKVREAERAQVVDAYRERLGEIISGTVKKVTRDNVIVDLGNNA
EALLAREDIISRETFRVGVRLRALLKEIRTENRGPQLILSRTAPEMLIELFRIEVPEIAEGLIEVMAASRDPGSRAKIAV
RSKDKRIDPQGACIGMRGSRVQAVSGELGGERVDIVLWDDNPAQFVINAMSPAEVAAIIVDEDAHAMDIAVGADNLAQAI
GRGGQNVRLASQLTGWTLNVMTESDIQAKQQAETGDILRNFIDELEVDEELAQVLVDEGFTSLEEIAYVPLEEMLNIDGF
DEDIVNELRARAKDRLLTKAIATEEKLADAHPAEDLLSLEGMDKDLAMELAVRGVITREDLAEQSIDDLLDIDGIDDDRA
GKLIMAARAHWFE
>Mature_492_residues
SKEVLLVVESVSNEKGVPASVIFEALELALATATKKRFEDEVDLRVEINRHTGSYETFRRWTVVEENDLDDPAIETWPSK
VAETHPGAKVGDVVEEKIESIEFGRIAAQTAKQVIVQKVREAERAQVVDAYRERLGEIISGTVKKVTRDNVIVDLGNNAE
ALLAREDIISRETFRVGVRLRALLKEIRTENRGPQLILSRTAPEMLIELFRIEVPEIAEGLIEVMAASRDPGSRAKIAVR
SKDKRIDPQGACIGMRGSRVQAVSGELGGERVDIVLWDDNPAQFVINAMSPAEVAAIIVDEDAHAMDIAVGADNLAQAIG
RGGQNVRLASQLTGWTLNVMTESDIQAKQQAETGDILRNFIDELEVDEELAQVLVDEGFTSLEEIAYVPLEEMLNIDGFD
EDIVNELRARAKDRLLTKAIATEEKLADAHPAEDLLSLEGMDKDLAMELAVRGVITREDLAEQSIDDLLDIDGIDDDRAG
KLIMAARAHWFE

Specific function: Participates in both the termination and antitermination of transcription. NusA binds directly to the core enzyme of the DNA-dependent RNA polymerase. NusA also interacts with lambda N protein, RNA, rho factor, and perhaps nusB. Binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=493, Percent_Identity=64.0973630831643, Blast_Score=622, Evalue=1e-179,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 54473; Mature: 54342

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: PS00037 MYB_1 ; PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEVLLVVESVSNEKGVPASVIFEALELALATATKKRFEDEVDLRVEINRHTGSYETFR
CCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHH
RWTVVEENDLDDPAIETWPSKVAETHPGAKVGDVVEEKIESIEFGRIAAQTAKQVIVQKV
EEEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REAERAQVVDAYRERLGEIISGTVKKVTRDNVIVDLGNNAEALLAREDIISRETFRVGVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHH
LRALLKEIRTENRGPQLILSRTAPEMLIELFRIEVPEIAEGLIEVMAASRDPGSRAKIAV
HHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEE
RSKDKRIDPQGACIGMRGSRVQAVSGELGGERVDIVLWDDNPAQFVINAMSPAEVAAIIV
ECCCCCCCCCCCEECCCCCEEEEECCCCCCCEEEEEEECCCCHHEEEECCCCCCEEEEEE
DEDAHAMDIAVGADNLAQAIGRGGQNVRLASQLTGWTLNVMTESDIQAKQQAETGDILRN
ECCCCEEEEEECHHHHHHHHHCCCCCEEEHHHCCCCEEEEEECCHHHHHHHHHHHHHHHH
FIDELEVDEELAQVLVDEGFTSLEEIAYVPLEEMLNIDGFDEDIVNELRARAKDRLLTKA
HHHHHCHHHHHHHHHHHCCCHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
IATEEKLADAHPAEDLLSLEGMDKDLAMELAVRGVITREDLAEQSIDDLLDIDGIDDDRA
HHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GKLIMAARAHWFE
CCEEEEHHHHCCC
>Mature Secondary Structure 
SKEVLLVVESVSNEKGVPASVIFEALELALATATKKRFEDEVDLRVEINRHTGSYETFR
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHH
RWTVVEENDLDDPAIETWPSKVAETHPGAKVGDVVEEKIESIEFGRIAAQTAKQVIVQKV
EEEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REAERAQVVDAYRERLGEIISGTVKKVTRDNVIVDLGNNAEALLAREDIISRETFRVGVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHH
LRALLKEIRTENRGPQLILSRTAPEMLIELFRIEVPEIAEGLIEVMAASRDPGSRAKIAV
HHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEE
RSKDKRIDPQGACIGMRGSRVQAVSGELGGERVDIVLWDDNPAQFVINAMSPAEVAAIIV
ECCCCCCCCCCCEECCCCCEEEEECCCCCCCEEEEEEECCCCHHEEEECCCCCCEEEEEE
DEDAHAMDIAVGADNLAQAIGRGGQNVRLASQLTGWTLNVMTESDIQAKQQAETGDILRN
ECCCCEEEEEECHHHHHHHHHCCCCCEEEHHHCCCCEEEEEECCHHHHHHHHHHHHHHHH
FIDELEVDEELAQVLVDEGFTSLEEIAYVPLEEMLNIDGFDEDIVNELRARAKDRLLTKA
HHHHHCHHHHHHHHHHHCCCHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
IATEEKLADAHPAEDLLSLEGMDKDLAMELAVRGVITREDLAEQSIDDLLDIDGIDDDRA
HHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GKLIMAARAHWFE
CCEEEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113180; 11677609 [H]