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The map label for this gene is glcA [H]

Identifier: 77456980

GI number: 77456980

Start: 885641

End: 887335

Strand: Reverse

Name: glcA [H]

Synonym: Pfl01_0753

Alternate gene names: 77456980

Gene position: 887335-885641 (Counterclockwise)

Preceding gene: 77456982

Following gene: 77456979

Centisome position: 13.78

GC content: 61.89

Gene sequence:

>1695_bases
ATGCAAACCTGGCAACAGCTCTACAGCCCGCTCGGCAGTCTCGGCGTGTCCGCACTCGCGGCCGTCATCCCCATCGTTTT
CTTCTTCCTTGCCCTGGCGGTGTTCCGCCTCAAAGGTCATGTGGCCGGCAGCATCACGCTCGCCCTGGCCATCGCCGTGG
CGATCTTCGCGTTCCAGATGCCGGTCGACATGGCGTTCGCCGCCGCCGGATATGGCTTCGCCTACGGCCTGTGGCCGATT
GCCTGGATCATTGTGGCGGCGGTGTTCCTGTACAAACTGACGGTCAAGAGCGGTCAGTTCGAGGTCATCCGCAGCTCGGT
GCTGTCGATCACTGACGACCAACGCTTGCAGGTGCTGCTGATCGGTTTCTGCTTCGGTGCATTCCTGGAAGGCGCCGCCG
GTTTCGGTGCGCCCGTGGCGATTACCGCTGCGCTGCTCGTAGGCCTGGGTTTCAACCCGTTGTACGCCGCCGGCCTGTGC
CTGATCGCCAACACCGCACCGGTGGCGTTCGGCGCACTGGGGATTCCGATCATCGTGGCCGGGCAAGTCACCGGCATCGA
CGCGTTCAAGATCGGCGCCATGACCGGCCGCCAGCTGCCACTGCTGTCGCTGTTCGTGCCGTTCTGGCTGGTGTTCATGA
TGGATGGCCTGCGCGGCGTGCGTGAAACCTGGCCAGCCGCACTGGTTGCCGGCCTGAGCTTCGCCGTGACTCAATACTTC
ACTTCGAACTTCATCGGCCCGGAACTGCCGGACATCACGTCGGCCCTGGCCAGCCTGATTTCGCTGACCCTGTTCCTGAA
GGTCTGGCAGCCAAAACGTGCCGCCGGCCAGCACATTGCCGGTGCCGTTTCCGCTTCGGTGGTAACTGCCAGCGTCGGCG
GTTTCGGCCAGAAGCGCACCACAGTTGCTTCGCCTTACAGCCTCGGGGAAATTTTCAAGGCGTGGTCGCCGTTCCTGATC
CTCACCGTGCTGGTGACCATCTGGACGCTCAAGCCCTTCAAGGCCATGTTCGCCGCCGGCGGTTCGATGTACGCCTGGGT
GTTCAACTTCGCGATTCCGCACCTTGATCAACTGGTGATCAAGACCGCACCGATCGTGGCCGCCCCGACCGCGATCCCGG
CCGTGTTCAAACTCGATCCGATTTCCGCGACCGGCACGGCGATTTTCTTCTCCGCGCTGATCTCGATGCTGGTGCTGAAG
ATCAATTTCAAAACTGGTCTGACCACTTTGAGAGAGACCTTCTACGAACTGCGCTGGCCGATCCTGTCGATCGGCATGGT
GTTGGCGTTTGCCTTCGTCACCAACTATTCCGGCATGTCCTCGACCATGGCTCTGGTGCTGGCGGCGACCGGCGCGGCGT
TTCCGTTCTTCTCGCCTTTCCTCGGCTGGCTCGGCGTGTTCCTGACCGGTTCCGATACTTCGTCCAACGCCCTGTTCAGT
TCGCTGCAAGCGACCACCGCGCACCAGATCGGGGTCAACGACACTTTGCTGGTGGCCGCCAATACCAGCGGCGGTGTGAC
CGGCAAGATGATCTCGCCACAATCGATCGCCGTGGCCTGCGCCGCGACCGGTCTGGTGGGCAAGGAATCCGATCTGTTCC
GCTTCACCCTCAAGCACAGCCTATTCTTTGCCACGATCGTCGGCCTGATCACTTTGGCTCAGGCCTACTGGTTCACTGGC
ATGCTGGTGCATTAA

Upstream 100 bases:

>100_bases
CAAGGCTTTTACCCTAGCCAACAGCGATTGTAAATTGGTCTTACCAATTAACCAAGAACGCTGACAGTGCCTGACCAACA
ACAATTAGGGGCCACCCCAT

Downstream 100 bases:

>100_bases
GACTACAAGCGACATGGAAATAACCGACGCCGGTTCGTGATTCCGGCGTCAGCAATTCACAACCCGGTCTGTAAGGCTGC
TGAAAGCAACGCGCTCTATA

Product: L-lactate permease

Products: lactate [Cytoplasm]; Proton [Cytoplasm]; glycolate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 564; Mature: 564

Protein sequence:

>564_residues
MQTWQQLYSPLGSLGVSALAAVIPIVFFFLALAVFRLKGHVAGSITLALAIAVAIFAFQMPVDMAFAAAGYGFAYGLWPI
AWIIVAAVFLYKLTVKSGQFEVIRSSVLSITDDQRLQVLLIGFCFGAFLEGAAGFGAPVAITAALLVGLGFNPLYAAGLC
LIANTAPVAFGALGIPIIVAGQVTGIDAFKIGAMTGRQLPLLSLFVPFWLVFMMDGLRGVRETWPAALVAGLSFAVTQYF
TSNFIGPELPDITSALASLISLTLFLKVWQPKRAAGQHIAGAVSASVVTASVGGFGQKRTTVASPYSLGEIFKAWSPFLI
LTVLVTIWTLKPFKAMFAAGGSMYAWVFNFAIPHLDQLVIKTAPIVAAPTAIPAVFKLDPISATGTAIFFSALISMLVLK
INFKTGLTTLRETFYELRWPILSIGMVLAFAFVTNYSGMSSTMALVLAATGAAFPFFSPFLGWLGVFLTGSDTSSNALFS
SLQATTAHQIGVNDTLLVAANTSGGVTGKMISPQSIAVACAATGLVGKESDLFRFTLKHSLFFATIVGLITLAQAYWFTG
MLVH

Sequences:

>Translated_564_residues
MQTWQQLYSPLGSLGVSALAAVIPIVFFFLALAVFRLKGHVAGSITLALAIAVAIFAFQMPVDMAFAAAGYGFAYGLWPI
AWIIVAAVFLYKLTVKSGQFEVIRSSVLSITDDQRLQVLLIGFCFGAFLEGAAGFGAPVAITAALLVGLGFNPLYAAGLC
LIANTAPVAFGALGIPIIVAGQVTGIDAFKIGAMTGRQLPLLSLFVPFWLVFMMDGLRGVRETWPAALVAGLSFAVTQYF
TSNFIGPELPDITSALASLISLTLFLKVWQPKRAAGQHIAGAVSASVVTASVGGFGQKRTTVASPYSLGEIFKAWSPFLI
LTVLVTIWTLKPFKAMFAAGGSMYAWVFNFAIPHLDQLVIKTAPIVAAPTAIPAVFKLDPISATGTAIFFSALISMLVLK
INFKTGLTTLRETFYELRWPILSIGMVLAFAFVTNYSGMSSTMALVLAATGAAFPFFSPFLGWLGVFLTGSDTSSNALFS
SLQATTAHQIGVNDTLLVAANTSGGVTGKMISPQSIAVACAATGLVGKESDLFRFTLKHSLFFATIVGLITLAQAYWFTG
MLVH
>Mature_564_residues
MQTWQQLYSPLGSLGVSALAAVIPIVFFFLALAVFRLKGHVAGSITLALAIAVAIFAFQMPVDMAFAAAGYGFAYGLWPI
AWIIVAAVFLYKLTVKSGQFEVIRSSVLSITDDQRLQVLLIGFCFGAFLEGAAGFGAPVAITAALLVGLGFNPLYAAGLC
LIANTAPVAFGALGIPIIVAGQVTGIDAFKIGAMTGRQLPLLSLFVPFWLVFMMDGLRGVRETWPAALVAGLSFAVTQYF
TSNFIGPELPDITSALASLISLTLFLKVWQPKRAAGQHIAGAVSASVVTASVGGFGQKRTTVASPYSLGEIFKAWSPFLI
LTVLVTIWTLKPFKAMFAAGGSMYAWVFNFAIPHLDQLVIKTAPIVAAPTAIPAVFKLDPISATGTAIFFSALISMLVLK
INFKTGLTTLRETFYELRWPILSIGMVLAFAFVTNYSGMSSTMALVLAATGAAFPFFSPFLGWLGVFLTGSDTSSNALFS
SLQATTAHQIGVNDTLLVAANTSGGVTGKMISPQSIAVACAATGLVGKESDLFRFTLKHSLFFATIVGLITLAQAYWFTG
MLVH

Specific function: Transports glycolate across the membrane. Can also transport L-lactate and D-lactate. Seems to be driven by a proton motive force [H]

COG id: COG1620

COG function: function code C; L-lactate permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lactate permease family [H]

Homologues:

Organism=Escherichia coli, GI1789347, Length=565, Percent_Identity=75.0442477876106, Blast_Score=817, Evalue=0.0,
Organism=Escherichia coli, GI1790031, Length=562, Percent_Identity=64.4128113879004, Blast_Score=693, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003804 [H]

Pfam domain/function: PF02652 Lactate_perm [H]

EC number: NA

Molecular weight: Translated: 59642; Mature: 59642

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHCEECC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactate [Periplasm]; Proton [Periplasm]; glycolate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactate [Periplasm] = Proton [Cytoplasm] + lactate [Cytoplasm] Proton [Periplasm] + glycolate [Periplasm] = Proton [Cytoplasm] + glycolate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 11283302; 11785976 [H]