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The map label for this gene is rutR [H]

Identifier: 77456911

GI number: 77456911

Start: 797669

End: 798319

Strand: Reverse

Name: rutR [H]

Synonym: Pfl01_0683

Alternate gene names: 77456911

Gene position: 798319-797669 (Counterclockwise)

Preceding gene: 77456912

Following gene: 77456910

Centisome position: 12.4

GC content: 59.29

Gene sequence:

>651_bases
ATGACCTTTGAAGTCCCAGCCCACGGCGGCAAACCCGCCAGCCGCATTCGTCAGAAGAACGAAGAGACCATCATCAAAGC
CGCCGAAGACGAGTTCGCCCGTCACGGGTACAAAGGCACAAGCATGAACACCATCGCCACCAAGGCCGGACTGCCCAAGG
CGAACCTGCATTACTACTTCACCAACAAGCTCGGGTTGTACGTGGCGGTGCTGAGCAACATCCTTCAATTGTGGGACAGC
ACCTTCAATACCCTGACGGCCGAGGACGATCCGGCCGAAGCGCTGACCCGCTACATCCGCGCGAAAATGGAATTCTCCCG
TCGCCAGCCGCAAGCGTCGCGGATCTTCGCGATGGAAGTGATCAGCGGCGGCGAATGCCTGACCGAATATTTCAATCAGG
ATTACCGCGCCTGGTTCCAGGGCCGCGCCGGCGTGTTCCAGGCCTGGATCGACGCGGGCAAGATGGACCCGGTCGACCCG
GTGCACCTGATCTTCCTGTTGTGGGGCAGCACCCAGCATTACGCCGACTTCGCCACCCAGATCTGCCGGGTGACCGGTCG
CAGCAAGTTGACCAAACAGGATATGGAAGACGCCGGCAACAACCTGATCCGCATCATCCTCAAAGGCTGCGGCCTCACTT
CTTCTATATAA

Upstream 100 bases:

>100_bases
GCTGACGACCGGGGTTTTATTCGCCCGGGGCTTGTCCCGCGCCGGTGGTTGCGCTATCAACGCAACTGGTTGCACCCCAC
AGACAAACCCGGAAGTGCCT

Downstream 100 bases:

>100_bases
GTCGTTTTATGCCATTCACCCTCAGCGGCTTTTGCGAATTCCGCGAAGAGATTCGCAAAAGCCGCTTCATCACCTTCGCC
GCGCCGATCACCAGCCCCGC

Product: TetR family transcriptional regulator

Products: NA

Alternate protein names: Rut operon repressor [H]

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MTFEVPAHGGKPASRIRQKNEETIIKAAEDEFARHGYKGTSMNTIATKAGLPKANLHYYFTNKLGLYVAVLSNILQLWDS
TFNTLTAEDDPAEALTRYIRAKMEFSRRQPQASRIFAMEVISGGECLTEYFNQDYRAWFQGRAGVFQAWIDAGKMDPVDP
VHLIFLLWGSTQHYADFATQICRVTGRSKLTKQDMEDAGNNLIRIILKGCGLTSSI

Sequences:

>Translated_216_residues
MTFEVPAHGGKPASRIRQKNEETIIKAAEDEFARHGYKGTSMNTIATKAGLPKANLHYYFTNKLGLYVAVLSNILQLWDS
TFNTLTAEDDPAEALTRYIRAKMEFSRRQPQASRIFAMEVISGGECLTEYFNQDYRAWFQGRAGVFQAWIDAGKMDPVDP
VHLIFLLWGSTQHYADFATQICRVTGRSKLTKQDMEDAGNNLIRIILKGCGLTSSI
>Mature_215_residues
TFEVPAHGGKPASRIRQKNEETIIKAAEDEFARHGYKGTSMNTIATKAGLPKANLHYYFTNKLGLYVAVLSNILQLWDST
FNTLTAEDDPAEALTRYIRAKMEFSRRQPQASRIFAMEVISGGECLTEYFNQDYRAWFQGRAGVFQAWIDAGKMDPVDPV
HLIFLLWGSTQHYADFATQICRVTGRSKLTKQDMEDAGNNLIRIILKGCGLTSSI

Specific function: Master transcription regulator which represses the degradation of pyrimidines (rutABCDEFG) and purines (gcl operon) for maintenance of metabolic balance between pyrimidines and purines. It also regulates the synthesis of pyrimidine nucleotides and arginin

COG id: COG1309

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787249, Length=197, Percent_Identity=40.1015228426396, Blast_Score=164, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR019915
- InterPro:   IPR001647
- InterPro:   IPR013573 [H]

Pfam domain/function: PF08362 TetR_C_3; PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 24308; Mature: 24177

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFEVPAHGGKPASRIRQKNEETIIKAAEDEFARHGYKGTSMNTIATKAGLPKANLHYYF
CEEECCCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCEEEEE
TNKLGLYVAVLSNILQLWDSTFNTLTAEDDPAEALTRYIRAKMEFSRRQPQASRIFAMEV
ECHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ISGGECLTEYFNQDYRAWFQGRAGVFQAWIDAGKMDPVDPVHLIFLLWGSTQHYADFATQ
HCCHHHHHHHHCHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHH
ICRVTGRSKLTKQDMEDAGNNLIRIILKGCGLTSSI
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TFEVPAHGGKPASRIRQKNEETIIKAAEDEFARHGYKGTSMNTIATKAGLPKANLHYYF
EEECCCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCEEEEE
TNKLGLYVAVLSNILQLWDSTFNTLTAEDDPAEALTRYIRAKMEFSRRQPQASRIFAMEV
ECHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ISGGECLTEYFNQDYRAWFQGRAGVFQAWIDAGKMDPVDPVHLIFLLWGSTQHYADFATQ
HCCHHHHHHHHCHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHH
ICRVTGRSKLTKQDMEDAGNNLIRIILKGCGLTSSI
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]