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The map label for this gene is ynfL [H]

Identifier: 77456645

GI number: 77456645

Start: 478941

End: 479837

Strand: Direct

Name: ynfL [H]

Synonym: Pfl01_0417

Alternate gene names: 77456645

Gene position: 478941-479837 (Clockwise)

Preceding gene: 77456643

Following gene: 77456647

Centisome position: 7.44

GC content: 64.21

Gene sequence:

>897_bases
ATGGAATTGCGTCATCTGCGCTACTTCATCGCCGTCGCCGAAGAACTGCACTTCGGCCGCGCCGCTCAGGTGCTGGGCAT
CTCCCAGCCACCGCTGAGCCAGCAGATCCAGGCGCTGGAACAGGAAGTCGGCGCGCGACTGTTCGAGCGGACCAATCGTC
GGGTCGAGCTCAGCGAGGCCGGGCGACTGTTTCTGGAGGAAGCGCGGCTGGTGCTCGCGCAAGTCGATAAAGCGGCCGAC
GTGGCTCGCCGTGCACAACTCGGCGAGCTGGGTGAGCTGAAGATCGGCTTCACCTCGTCGGCGCCGTTCAACTCGACCAT
TCCCCAGGCGATTTTTTCCTTCCGCCAGCGCTTCCCCGCCGTGCACCTGAATCTGCGCGAGATGAGCAGCACCATGGTCG
CCGACGCGCTGGTGGACGAATCGATCGAAGTCGGCATCATGCGACCGCTGGGTTTGCCGGATTCGCTCAGTGTGGTCGAA
CTGATGCGCGAGCCGCTGGTGGCCGTGCTCAGTTCCAAGCATCCGCTGGCGCAGGGCTCTGAAGAGGGGCTGTTCCTCTC
GGCGCTGGCCCTGGAGCCGTTCGTGTTTTTCCCGCGCAGTTACGGCAGTGGTCTGTATTCACAGTTGATCAGCCTGGCCC
GCGACGCGGGATTCAGTCCGCACTTTGCCCAGGAGGCCGGCGAAGCAATGACCATCATCGGTCTGGTGGCAGCCGGCCTG
GGCGTGTCGGTCATGCCGGCCTCGTATCAACGGATGCGCATCGACGGCGTGGTCTACCGACCGCTGCTCGATCCCGAGGC
CGTGACGGCGGTTTGGCTGGTGCAGCGCAAGGACCAGAAATCGCCGATGGCCAAGGCGTTTGTCGAACTGCTCACGCGCA
AGGTCGACGCCGGTTGA

Upstream 100 bases:

>100_bases
CGGGCAGACAGGCAATGAAAAAATCATATAGCTGGCTAATGTTTCTATCCAATATATTGTTCGACCTGTTTGATAGCTTT
TACGACCTAATGGGGTGCCC

Downstream 100 bases:

>100_bases
TCAGGGCAGCCGCACCAGATCACCCTTCAACGCCACCCGCGTGACAAAGATGCCGGCCCGACATTCGTAAGTGTTCAGAT
CCTTGCGCACATGCTGGTCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MELRHLRYFIAVAEELHFGRAAQVLGISQPPLSQQIQALEQEVGARLFERTNRRVELSEAGRLFLEEARLVLAQVDKAAD
VARRAQLGELGELKIGFTSSAPFNSTIPQAIFSFRQRFPAVHLNLREMSSTMVADALVDESIEVGIMRPLGLPDSLSVVE
LMREPLVAVLSSKHPLAQGSEEGLFLSALALEPFVFFPRSYGSGLYSQLISLARDAGFSPHFAQEAGEAMTIIGLVAAGL
GVSVMPASYQRMRIDGVVYRPLLDPEAVTAVWLVQRKDQKSPMAKAFVELLTRKVDAG

Sequences:

>Translated_298_residues
MELRHLRYFIAVAEELHFGRAAQVLGISQPPLSQQIQALEQEVGARLFERTNRRVELSEAGRLFLEEARLVLAQVDKAAD
VARRAQLGELGELKIGFTSSAPFNSTIPQAIFSFRQRFPAVHLNLREMSSTMVADALVDESIEVGIMRPLGLPDSLSVVE
LMREPLVAVLSSKHPLAQGSEEGLFLSALALEPFVFFPRSYGSGLYSQLISLARDAGFSPHFAQEAGEAMTIIGLVAAGL
GVSVMPASYQRMRIDGVVYRPLLDPEAVTAVWLVQRKDQKSPMAKAFVELLTRKVDAG
>Mature_298_residues
MELRHLRYFIAVAEELHFGRAAQVLGISQPPLSQQIQALEQEVGARLFERTNRRVELSEAGRLFLEEARLVLAQVDKAAD
VARRAQLGELGELKIGFTSSAPFNSTIPQAIFSFRQRFPAVHLNLREMSSTMVADALVDESIEVGIMRPLGLPDSLSVVE
LMREPLVAVLSSKHPLAQGSEEGLFLSALALEPFVFFPRSYGSGLYSQLISLARDAGFSPHFAQEAGEAMTIIGLVAAGL
GVSVMPASYQRMRIDGVVYRPLLDPEAVTAVWLVQRKDQKSPMAKAFVELLTRKVDAG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=288, Percent_Identity=50.3472222222222, Blast_Score=280, Evalue=6e-77,
Organism=Escherichia coli, GI1788887, Length=298, Percent_Identity=34.2281879194631, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI2367136, Length=264, Percent_Identity=34.469696969697, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1790399, Length=282, Percent_Identity=32.9787234042553, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI157672245, Length=305, Percent_Identity=31.4754098360656, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI145693105, Length=292, Percent_Identity=27.7397260273973, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787530, Length=271, Percent_Identity=29.520295202952, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788297, Length=299, Percent_Identity=27.4247491638796, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI87082132, Length=306, Percent_Identity=27.4509803921569, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87081904, Length=201, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1788748, Length=257, Percent_Identity=28.0155642023346, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790208, Length=290, Percent_Identity=27.2413793103448, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787601, Length=307, Percent_Identity=24.7557003257329, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789204, Length=268, Percent_Identity=27.6119402985075, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787806, Length=304, Percent_Identity=25, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788481, Length=247, Percent_Identity=26.3157894736842, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1786713, Length=178, Percent_Identity=28.0898876404494, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789173, Length=171, Percent_Identity=30.4093567251462, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32702; Mature: 32702

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRHLRYFIAVAEELHFGRAAQVLGISQPPLSQQIQALEQEVGARLFERTNRRVELSEA
CCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHH
GRLFLEEARLVLAQVDKAADVARRAQLGELGELKIGFTSSAPFNSTIPQAIFSFRQRFPA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCE
VHLNLREMSSTMVADALVDESIEVGIMRPLGLPDSLSVVELMREPLVAVLSSKHPLAQGS
EEEEHHHHHHHHHHHHHHHCCHHEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
EEGLFLSALALEPFVFFPRSYGSGLYSQLISLARDAGFSPHFAQEAGEAMTIIGLVAAGL
CCCEEEHHHHHCCHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHCC
GVSVMPASYQRMRIDGVVYRPLLDPEAVTAVWLVQRKDQKSPMAKAFVELLTRKVDAG
CCEECCCCHHHEEECCEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MELRHLRYFIAVAEELHFGRAAQVLGISQPPLSQQIQALEQEVGARLFERTNRRVELSEA
CCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHH
GRLFLEEARLVLAQVDKAADVARRAQLGELGELKIGFTSSAPFNSTIPQAIFSFRQRFPA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCE
VHLNLREMSSTMVADALVDESIEVGIMRPLGLPDSLSVVELMREPLVAVLSSKHPLAQGS
EEEEHHHHHHHHHHHHHHHCCHHEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
EEGLFLSALALEPFVFFPRSYGSGLYSQLISLARDAGFSPHFAQEAGEAMTIIGLVAAGL
CCCEEEHHHHHCCHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHCC
GVSVMPASYQRMRIDGVVYRPLLDPEAVTAVWLVQRKDQKSPMAKAFVELLTRKVDAG
CCEECCCCHHHEEECCEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]