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The map label for this gene is gltD [H]

Identifier: 77456642

GI number: 77456642

Start: 474764

End: 476182

Strand: Direct

Name: gltD [H]

Synonym: Pfl01_0414

Alternate gene names: 77456642

Gene position: 474764-476182 (Clockwise)

Preceding gene: 77456641

Following gene: 77456643

Centisome position: 7.37

GC content: 62.37

Gene sequence:

>1419_bases
ATGGCTGAACGTCTGAATAACGACTTCCAGTTCATCGATGTCGGGCGCAAGGATCCGAAGAAGAAACTGTTGCGTCAACG
CAAGAAAGAGTTCGTGGAAATCTACGAACCGTTCAAACCCCAGCAGTCGGCCGACCAGGCTCACCGCTGTCTGGGTTGCG
GCAACCCGTATTGCGAATGGAAGTGCCCGGTGCACAACTTCATTCCGAACTGGCTGAAGCTGGTGGCCGAGGGCAACATC
CTCCAGGCCGCCGAGCTGTCGCACCAGACCAACACCCTGCCGGAAGTCTGCGGCCGGGTGTGCCCGCAGGATCGTCTGTG
CGAGGGTGCGTGCACCCTCAACGACGGCTTCGGCGCGGTGACCATCGGTTCGGTCGAGAAGTACATCACCGACACCGCGT
TCGCCATGGGCTGGCGCCCGGACATGTCCAAGGTCAAACCGACCGGCAAGCGTGTCGCGATCATCGGCGCGGGCCCGGCG
GGCCTCGGTTGTGCCGACGTGCTGGTGCGCGGCGGCGTGACCCCGGTGGTGTTCGACAAGAACCCGGAAATCGGTGGTCT
GCTGACCTTCGGCATCCCCGAGTTCAAGCTCGAGAAGACCGTGCTGAGCAATCGCCGCGAAGTCTTCACCGGCATGGGCA
TCGAGTTCCGCCTCAACACCGAGGTCGGCAAGGACGTGACCATGGAGCAACTGCTCGCCGAATACGATGCCGTGTTCATG
GGCATGGGCACCTACACCTACATGAAGGGCGGTTTTGCCGGTGAGGATCTGCCGGGCGTTTATGACGCGCTGGATTTCCT
GATCGCCAACGTCAACCGCAACCTGGGCTTTGAAAAGTCGCCGGAAGATTTCGTCGACATGAAGGGCAAGAAGGTCGTGG
TGCTGGGTGGCGGCGACACGGCGATGGACTGCAACCGCACGTCGATCCGCCAGGGCGCCAAATCGGTGACCTGCGCCTAT
CGTCGTGACGAAGCGAACATGCCCGGCTCGCGCAAAGAGGTGAAAAACGCCAAGGAAGAAGGCGTGAAATTCCTCTACAA
CCGCCAGCCGATCGCCATCGTCGGCGAGGACAAGGTCGAAGGCGTGAAGGTGGTCGAGACCCGTCTCGGCGAACCGGATG
CCCGTGGCCGTCGCAGCCCCGAGCCGATCCCGGGCTCCGAAGAGATCATCCCGGCCGACGCCGTGGTCATCGCGTTCGGT
TTCCGCCCGAGCCCGGCGCCATGGTTCGAACAGTTCGAAATCCAGACCGACAGCCAGGGCCGCGTCGTTGCGCCCGAGCA
AGGTCAGTACAAGCACCAGACCAGCAACCCGAAAATCTTCGCCGGTGGCGACATGGTGCGCGGTTCCGACCTGGTGGTGA
CGGCGATCTTCGAAGGCCGCAATGCGGCTGAAGGGATCCTGGACTACCTGGGCGTCTAA

Upstream 100 bases:

>100_bases
GCTGGTCAAGCCGAAGGCTGCCAACCTGAAGTCGTTGCTTTCCAGCACCCGTGCCAACCCGCAGTGATATGCGCCTGAAG
AGTTTGATGAGGTTTTAACA

Downstream 100 bases:

>100_bases
ACCTTCCTCTGATCGTTCCCACGCTCTGCGTGGGAATGCCTCCCGGGACGCTCTGCGTCCCAAAAGGCGTGACGCGGAGC
GTCACTGGCGGCGTTACCAC

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MAERLNNDFQFIDVGRKDPKKKLLRQRKKEFVEIYEPFKPQQSADQAHRCLGCGNPYCEWKCPVHNFIPNWLKLVAEGNI
LQAAELSHQTNTLPEVCGRVCPQDRLCEGACTLNDGFGAVTIGSVEKYITDTAFAMGWRPDMSKVKPTGKRVAIIGAGPA
GLGCADVLVRGGVTPVVFDKNPEIGGLLTFGIPEFKLEKTVLSNRREVFTGMGIEFRLNTEVGKDVTMEQLLAEYDAVFM
GMGTYTYMKGGFAGEDLPGVYDALDFLIANVNRNLGFEKSPEDFVDMKGKKVVVLGGGDTAMDCNRTSIRQGAKSVTCAY
RRDEANMPGSRKEVKNAKEEGVKFLYNRQPIAIVGEDKVEGVKVVETRLGEPDARGRRSPEPIPGSEEIIPADAVVIAFG
FRPSPAPWFEQFEIQTDSQGRVVAPEQGQYKHQTSNPKIFAGGDMVRGSDLVVTAIFEGRNAAEGILDYLGV

Sequences:

>Translated_472_residues
MAERLNNDFQFIDVGRKDPKKKLLRQRKKEFVEIYEPFKPQQSADQAHRCLGCGNPYCEWKCPVHNFIPNWLKLVAEGNI
LQAAELSHQTNTLPEVCGRVCPQDRLCEGACTLNDGFGAVTIGSVEKYITDTAFAMGWRPDMSKVKPTGKRVAIIGAGPA
GLGCADVLVRGGVTPVVFDKNPEIGGLLTFGIPEFKLEKTVLSNRREVFTGMGIEFRLNTEVGKDVTMEQLLAEYDAVFM
GMGTYTYMKGGFAGEDLPGVYDALDFLIANVNRNLGFEKSPEDFVDMKGKKVVVLGGGDTAMDCNRTSIRQGAKSVTCAY
RRDEANMPGSRKEVKNAKEEGVKFLYNRQPIAIVGEDKVEGVKVVETRLGEPDARGRRSPEPIPGSEEIIPADAVVIAFG
FRPSPAPWFEQFEIQTDSQGRVVAPEQGQYKHQTSNPKIFAGGDMVRGSDLVVTAIFEGRNAAEGILDYLGV
>Mature_471_residues
AERLNNDFQFIDVGRKDPKKKLLRQRKKEFVEIYEPFKPQQSADQAHRCLGCGNPYCEWKCPVHNFIPNWLKLVAEGNIL
QAAELSHQTNTLPEVCGRVCPQDRLCEGACTLNDGFGAVTIGSVEKYITDTAFAMGWRPDMSKVKPTGKRVAIIGAGPAG
LGCADVLVRGGVTPVVFDKNPEIGGLLTFGIPEFKLEKTVLSNRREVFTGMGIEFRLNTEVGKDVTMEQLLAEYDAVFMG
MGTYTYMKGGFAGEDLPGVYDALDFLIANVNRNLGFEKSPEDFVDMKGKKVVVLGGGDTAMDCNRTSIRQGAKSVTCAYR
RDEANMPGSRKEVKNAKEEGVKFLYNRQPIAIVGEDKVEGVKVVETRLGEPDARGRRSPEPIPGSEEIIPADAVVIAFGF
RPSPAPWFEQFEIQTDSQGRVVAPEQGQYKHQTSNPKIFAGGDMVRGSDLVVTAIFEGRNAAEGILDYLGV

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=445, Percent_Identity=28.9887640449438, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI111118983, Length=251, Percent_Identity=27.0916334661355, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI111118981, Length=158, Percent_Identity=31.0126582278481, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1789606, Length=469, Percent_Identity=69.9360341151386, Blast_Score=680, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=462, Percent_Identity=61.6883116883117, Blast_Score=604, Evalue=1e-174,
Organism=Escherichia coli, GI87082180, Length=456, Percent_Identity=56.359649122807, Blast_Score=525, Evalue=1e-150,
Organism=Escherichia coli, GI1788468, Length=440, Percent_Identity=31.3636363636364, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1789243, Length=419, Percent_Identity=29.3556085918854, Blast_Score=134, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17570289, Length=496, Percent_Identity=36.6935483870968, Blast_Score=253, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI71984108, Length=465, Percent_Identity=29.4623655913978, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17543860, Length=221, Percent_Identity=27.1493212669683, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320030, Length=492, Percent_Identity=32.1138211382114, Blast_Score=226, Evalue=5e-60,
Organism=Drosophila melanogaster, GI28574881, Length=482, Percent_Identity=38.3817427385892, Blast_Score=285, Evalue=4e-77,
Organism=Drosophila melanogaster, GI24665539, Length=482, Percent_Identity=38.3817427385892, Blast_Score=285, Evalue=4e-77,
Organism=Drosophila melanogaster, GI24665547, Length=482, Percent_Identity=38.3817427385892, Blast_Score=285, Evalue=5e-77,
Organism=Drosophila melanogaster, GI24665543, Length=482, Percent_Identity=38.3817427385892, Blast_Score=285, Evalue=5e-77,
Organism=Drosophila melanogaster, GI24640763, Length=444, Percent_Identity=27.4774774774775, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI18858217, Length=444, Percent_Identity=27.4774774774775, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 51848; Mature: 51717

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERLNNDFQFIDVGRKDPKKKLLRQRKKEFVEIYEPFKPQQSADQAHRCLGCGNPYCEW
CCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEE
KCPVHNFIPNWLKLVAEGNILQAAELSHQTNTLPEVCGRVCPQDRLCEGACTLNDGFGAV
ECCHHHHHHHHHHHHHCCCEEEHHHHHHCCCCHHHHHHHHCCHHHHCCCCEEECCCCCEE
TIGSVEKYITDTAFAMGWRPDMSKVKPTGKRVAIIGAGPAGLGCADVLVRGGVTPVVFDK
EECCHHHHHHHHHHHCCCCCCHHHCCCCCCEEEEEECCCCCCCHHHHHHHCCCCEEEECC
NPEIGGLLTFGIPEFKLEKTVLSNRREVFTGMGIEFRLNTEVGKDVTMEQLLAEYDAVFM
CCCCCEEEEECCCCHHHHHHHHHCCHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHEE
GMGTYTYMKGGFAGEDLPGVYDALDFLIANVNRNLGFEKSPEDFVDMKGKKVVVLGGGDT
CCCHHHEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCEEEEECCCCC
AMDCNRTSIRQGAKSVTCAYRRDEANMPGSRKEVKNAKEEGVKFLYNRQPIAIVGEDKVE
CCCCCHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCHHEEECCCCEEEECCCCCC
GVKVVETRLGEPDARGRRSPEPIPGSEEIIPADAVVIAFGFRPSPAPWFEQFEIQTDSQG
CEEEEHHHCCCCCCCCCCCCCCCCCCCCEECCCEEEEEECCCCCCCCCHHEEEEEECCCC
RVVAPEQGQYKHQTSNPKIFAGGDMVRGSDLVVTAIFEGRNAAEGILDYLGV
CEECCCCCCCCCCCCCCEEEECCCEECCCCEEEEEEECCCCHHHHHHHHHCC
>Mature Secondary Structure 
AERLNNDFQFIDVGRKDPKKKLLRQRKKEFVEIYEPFKPQQSADQAHRCLGCGNPYCEW
CCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEE
KCPVHNFIPNWLKLVAEGNILQAAELSHQTNTLPEVCGRVCPQDRLCEGACTLNDGFGAV
ECCHHHHHHHHHHHHHCCCEEEHHHHHHCCCCHHHHHHHHCCHHHHCCCCEEECCCCCEE
TIGSVEKYITDTAFAMGWRPDMSKVKPTGKRVAIIGAGPAGLGCADVLVRGGVTPVVFDK
EECCHHHHHHHHHHHCCCCCCHHHCCCCCCEEEEEECCCCCCCHHHHHHHCCCCEEEECC
NPEIGGLLTFGIPEFKLEKTVLSNRREVFTGMGIEFRLNTEVGKDVTMEQLLAEYDAVFM
CCCCCEEEEECCCCHHHHHHHHHCCHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHEE
GMGTYTYMKGGFAGEDLPGVYDALDFLIANVNRNLGFEKSPEDFVDMKGKKVVVLGGGDT
CCCHHHEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCEEEEECCCCC
AMDCNRTSIRQGAKSVTCAYRRDEANMPGSRKEVKNAKEEGVKFLYNRQPIAIVGEDKVE
CCCCCHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCHHEEECCCCEEEECCCCCC
GVKVVETRLGEPDARGRRSPEPIPGSEEIIPADAVVIAFGFRPSPAPWFEQFEIQTDSQG
CEEEEHHHCCCCCCCCCCCCCCCCCCCCEECCCEEEEEECCCCCCCCCHHEEEEEECCCC
RVVAPEQGQYKHQTSNPKIFAGGDMVRGSDLVVTAIFEGRNAAEGILDYLGV
CEECCCCCCCCCCCCCCEEEECCCEECCCCEEEEEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]