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The map label for this gene is maeB [H]

Identifier: 77456631

GI number: 77456631

Start: 457469

End: 458821

Strand: Direct

Name: maeB [H]

Synonym: Pfl01_0403

Alternate gene names: 77456631

Gene position: 457469-458821 (Clockwise)

Preceding gene: 77456630

Following gene: 77456633

Centisome position: 7.11

GC content: 59.87

Gene sequence:

>1353_bases
GTGACCGGGCAGTCTTGTGGGGACTTTGCGAGGCGCGTATCCTCGCTGACCAGTCTGTCCAACAGTAAAAAGCGGAATGC
CAAAATGTCTGATCTGAAAACTGCCGCTCTCGAATATCATGCCAATCCTCGTCCAGGAAAGCTGAGTGTCGAGCTCACCA
AGGCCACTGCTACCGCCCGCGACCTGTCGCTGGCCTACAGCCCCGGCGTAGCTGAACCAGTGCGCGAGATCGCCCGCGAT
CCTGAACTGGCCTACAAGTACACCGGCAAGGGCAACCTGGTTGCAGTCATTTCCGATGGCACCGCGATTCTGGGCCTGGG
TAACCTCGGCCCACTGGCTTCCAAGCCAGTCATGGAAGGTAAAGGCGTGCTGTTCAAGCGCTTCGCCGGCATCGACGTCT
TCGACATTGAAGTCGACTCCGAGAGCCCGCAAGCCTTCATCGACACCGTAAAACGCATTTCCATCACCTTCGGTGGCATC
AACCTGGAAGACATCAAGGCACCTGAGTGCTTTGAGATCGAACGCGCTCTGATCGAGCAGTGCGACATTCCGGTCTTCCA
CGATGACCAGCACGGCACCGCGATCGTGACCGCTGCGGGCATGATCAACGCCCTGGAAATCGCGGGCAAAACCCTGGCCG
ACGCCAAGATCGTCTGCCTGGGCGCTGGCGCCGCCGCCATCTCCTGCATGAAATTGCTGGTGAGCATGGGCGCCAAGGTC
GAAAACATCTTCATGGTTGACCGTACCGGCGTGATCCACGCTGGCCGTGACGACCTGAACCAGTACAAGGCTGTGTTCGC
TCACCCTACCGAGAAACGCACCCTGGACGACGCCATCGAAGGTGCTGACGTATTCGTCGGCCTGTCGGGTCCAAACCTGC
TGAGCGCCGAGCAACTCAAGCGCATGGCAGCCAACCCGATCGTCTTCGCCTGCTCCAACCCGGATCCGGAAATCTCCCCG
GAACTGGCTCATGCCACCCGTAACGACGTGATCATGGCCACCGGCCGTTCGGACTACCCGAACCAGGTCAACAACGTGCT
GGGCTTCCCGTTCATCTTCCGTGGTGCCCTGGACGTTCGCGCCAAGCGCATCAACGAAGAAATGAAGATCGCTGCCGCCA
ACGCCCTGCGCGAACTGGCCAAGCTGCCGGTCCCGCAGGAAGTCTGCGACGCCTACGGCGGTATCTCCCTGGAATTCGGC
CGTGAGTACATCATTCCGAAGCCAATGGACAAACGCCTGATCACCGTGATCTCCGACGCCGTGGCCAAGGCCGCGATCGA
GACTGGTGTGGCGACCCTGCCGTATCCGAAGAACTACCCGCTGAAAAGCGTGGATGACGTGTTCAACGGCTAA

Upstream 100 bases:

>100_bases
ATAAAAAGTTGTAGACATTTTTTCTGTCGATTGTGAACAGTCTAACCCTTGTGCGCCGTGGCTCTTGGCCCAAAGTCGTA
GGGCAGGGCGCTTGACAGGG

Downstream 100 bases:

>100_bases
GTCGTTGTAGCGCTTCAAGCGAAAGCCCTGACTCGTGAGAGTCGGGGCTTTTTTATGCCTGTTGATTTTGGGGTGATCTT
AAGGGCCTCTTCGCGAGCTT

Product: malic enzyme

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MTGQSCGDFARRVSSLTSLSNSKKRNAKMSDLKTAALEYHANPRPGKLSVELTKATATARDLSLAYSPGVAEPVREIARD
PELAYKYTGKGNLVAVISDGTAILGLGNLGPLASKPVMEGKGVLFKRFAGIDVFDIEVDSESPQAFIDTVKRISITFGGI
NLEDIKAPECFEIERALIEQCDIPVFHDDQHGTAIVTAAGMINALEIAGKTLADAKIVCLGAGAAAISCMKLLVSMGAKV
ENIFMVDRTGVIHAGRDDLNQYKAVFAHPTEKRTLDDAIEGADVFVGLSGPNLLSAEQLKRMAANPIVFACSNPDPEISP
ELAHATRNDVIMATGRSDYPNQVNNVLGFPFIFRGALDVRAKRINEEMKIAAANALRELAKLPVPQEVCDAYGGISLEFG
REYIIPKPMDKRLITVISDAVAKAAIETGVATLPYPKNYPLKSVDDVFNG

Sequences:

>Translated_450_residues
MTGQSCGDFARRVSSLTSLSNSKKRNAKMSDLKTAALEYHANPRPGKLSVELTKATATARDLSLAYSPGVAEPVREIARD
PELAYKYTGKGNLVAVISDGTAILGLGNLGPLASKPVMEGKGVLFKRFAGIDVFDIEVDSESPQAFIDTVKRISITFGGI
NLEDIKAPECFEIERALIEQCDIPVFHDDQHGTAIVTAAGMINALEIAGKTLADAKIVCLGAGAAAISCMKLLVSMGAKV
ENIFMVDRTGVIHAGRDDLNQYKAVFAHPTEKRTLDDAIEGADVFVGLSGPNLLSAEQLKRMAANPIVFACSNPDPEISP
ELAHATRNDVIMATGRSDYPNQVNNVLGFPFIFRGALDVRAKRINEEMKIAAANALRELAKLPVPQEVCDAYGGISLEFG
REYIIPKPMDKRLITVISDAVAKAAIETGVATLPYPKNYPLKSVDDVFNG
>Mature_449_residues
TGQSCGDFARRVSSLTSLSNSKKRNAKMSDLKTAALEYHANPRPGKLSVELTKATATARDLSLAYSPGVAEPVREIARDP
ELAYKYTGKGNLVAVISDGTAILGLGNLGPLASKPVMEGKGVLFKRFAGIDVFDIEVDSESPQAFIDTVKRISITFGGIN
LEDIKAPECFEIERALIEQCDIPVFHDDQHGTAIVTAAGMINALEIAGKTLADAKIVCLGAGAAAISCMKLLVSMGAKVE
NIFMVDRTGVIHAGRDDLNQYKAVFAHPTEKRTLDDAIEGADVFVGLSGPNLLSAEQLKRMAANPIVFACSNPDPEISPE
LAHATRNDVIMATGRSDYPNQVNNVLGFPFIFRGALDVRAKRINEEMKIAAANALRELAKLPVPQEVCDAYGGISLEFGR
EYIIPKPMDKRLITVISDAVAKAAIETGVATLPYPKNYPLKSVDDVFNG

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=405, Percent_Identity=28.1481481481481, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI62420882, Length=345, Percent_Identity=26.6666666666667, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI62420880, Length=345, Percent_Identity=26.6666666666667, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI239049447, Length=345, Percent_Identity=26.6666666666667, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI4505145, Length=346, Percent_Identity=27.7456647398844, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI270265879, Length=321, Percent_Identity=28.3489096573209, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1788806, Length=405, Percent_Identity=61.2345679012346, Blast_Score=489, Evalue=1e-139,
Organism=Escherichia coli, GI87081919, Length=288, Percent_Identity=28.125, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17537199, Length=346, Percent_Identity=28.6127167630058, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI78707242, Length=394, Percent_Identity=27.1573604060914, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI78707238, Length=394, Percent_Identity=27.1573604060914, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI78707240, Length=411, Percent_Identity=26.5206812652068, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24646388, Length=347, Percent_Identity=31.4121037463977, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24646386, Length=347, Percent_Identity=31.4121037463977, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281362674, Length=460, Percent_Identity=27.6086956521739, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356279, Length=460, Percent_Identity=27.6086956521739, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281362672, Length=460, Percent_Identity=27.6086956521739, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 48250; Mature: 48119

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGQSCGDFARRVSSLTSLSNSKKRNAKMSDLKTAALEYHANPRPGKLSVELTKATATAR
CCCCCHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHCCCCCCCCEEEEEEEECHHHHH
DLSLAYSPGVAEPVREIARDPELAYKYTGKGNLVAVISDGTAILGLGNLGPLASKPVMEG
HEEEEECCCCHHHHHHHHCCCCEEEEECCCCCEEEEEECCEEEEEECCCCCCCCCCCCCC
KGVLFKRFAGIDVFDIEVDSESPQAFIDTVKRISITFGGINLEDIKAPECFEIERALIEQ
CCHHHHHHCCCEEEEEEECCCCCHHHHHHHHHEEEEECCCCHHHCCCCCHHHHHHHHHHH
CDIPVFHDDQHGTAIVTAAGMINALEIAGKTLADAKIVCLGAGAAAISCMKLLVSMGAKV
CCCCEEECCCCCCEEEEHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCE
ENIFMVDRTGVIHAGRDDLNQYKAVFAHPTEKRTLDDAIEGADVFVGLSGPNLLSAEQLK
EEEEEEECCCEEECCHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEECCCCCCCCHHHHH
RMAANPIVFACSNPDPEISPELAHATRNDVIMATGRSDYPNQVNNVLGFPFIFRGALDVR
HHHCCCEEEEECCCCCCCCCHHHHCCCCCEEEEECCCCCCHHHHHHCCCHHHHHHHHHHH
AKRINEEMKIAAANALRELAKLPVPQEVCDAYGGISLEFGREYIIPKPMDKRLITVISDA
HHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCEEEECCCEECCCCCCCHHHHHHHHHH
VAKAAIETGVATLPYPKNYPLKSVDDVFNG
HHHHHHHHCCEECCCCCCCCCCHHHHHHCC
>Mature Secondary Structure 
TGQSCGDFARRVSSLTSLSNSKKRNAKMSDLKTAALEYHANPRPGKLSVELTKATATAR
CCCCHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHCCCCCCCCEEEEEEEECHHHHH
DLSLAYSPGVAEPVREIARDPELAYKYTGKGNLVAVISDGTAILGLGNLGPLASKPVMEG
HEEEEECCCCHHHHHHHHCCCCEEEEECCCCCEEEEEECCEEEEEECCCCCCCCCCCCCC
KGVLFKRFAGIDVFDIEVDSESPQAFIDTVKRISITFGGINLEDIKAPECFEIERALIEQ
CCHHHHHHCCCEEEEEEECCCCCHHHHHHHHHEEEEECCCCHHHCCCCCHHHHHHHHHHH
CDIPVFHDDQHGTAIVTAAGMINALEIAGKTLADAKIVCLGAGAAAISCMKLLVSMGAKV
CCCCEEECCCCCCEEEEHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCE
ENIFMVDRTGVIHAGRDDLNQYKAVFAHPTEKRTLDDAIEGADVFVGLSGPNLLSAEQLK
EEEEEEECCCEEECCHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEECCCCCCCCHHHHH
RMAANPIVFACSNPDPEISPELAHATRNDVIMATGRSDYPNQVNNVLGFPFIFRGALDVR
HHHCCCEEEEECCCCCCCCCHHHHCCCCCEEEEECCCCCCHHHHHHCCCHHHHHHHHHHH
AKRINEEMKIAAANALRELAKLPVPQEVCDAYGGISLEFGREYIIPKPMDKRLITVISDA
HHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCEEEECCCEECCCCCCCHHHHHHHHHH
VAKAAIETGVATLPYPKNYPLKSVDDVFNG
HHHHHHHHCCEECCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 36376 [H]