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The map label for this gene is insF1 [H]

Identifier: 77454821

GI number: 77454821

Start: 228089

End: 228598

Strand: Direct

Name: insF1 [H]

Synonym: pREL1_0254

Alternate gene names: 77454821

Gene position: 228089-228598 (Clockwise)

Preceding gene: 77454814

Following gene: 77454822

Centisome position: 83.99

GC content: 60.39

Gene sequence:

>510_bases
TTGCAACACCTTCCAGTAGGTGTTGCGACGATCGCTGGAATCCAAGCCGGTGAGGGGAAGCTGTATCTGTGTGCGATCAA
AGATGCCTGCGGTAATCGGATCGTCGGATACTCCATCGACTCGCGGATGAAAGCCCGCCGGGCGGTCACCGCTCTGAACA
ACGCCCTCGCTCGCCGCGGCGACGTCACCGGATGCGTGGTTCACTCGGACCGAGGCTCTCAATTTTCGTCCCGAAAGTTC
CTTAACGTCTTAACTACTCACGACCTGATCGGATCGATGGGCAAAGTCGGAGCCTGCGGCGACAACGCTGCAATGGAAAG
CTTTTTCTCGCTGTTGCAAAAGAACGTCCTCGACCGTCAGGCCTGGGCGACCCGCCAGGAGCTCCGAACAGCGATCGTCA
CCTGGATCGAGCGGACCTATCACCGCCGCCGACGCCAGGCCGCCCTCGGCCGATTGACCCCCATCGAGTACGAGACCATC
ATGAACACACCAGCCGCACCGGCTGCGTGA

Upstream 100 bases:

>100_bases
TGAACGCCGACCGCTCGCGCTGCGGCGCGGACCGTGCCACCTCTATCGAGTAGTCGGAAGAATTCTTCCTTCTGCTGATC
CGTGTACTTTTTCCCAGCCA

Downstream 100 bases:

>100_bases
CTACAACTGTCACCTATCCGTGCAGCAGACCCAATCTTGTTTCCTTGTGATCGTGGCCGATGCGTATGCCGTCATCTTTG
GCTTGAAATGCGCTGCGACT

Product: putative transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MQHLPVGVATIAGIQAGEGKLYLCAIKDACGNRIVGYSIDSRMKARRAVTALNNALARRGDVTGCVVHSDRGSQFSSRKF
LNVLTTHDLIGSMGKVGACGDNAAMESFFSLLQKNVLDRQAWATRQELRTAIVTWIERTYHRRRRQAALGRLTPIEYETI
MNTPAAPAA

Sequences:

>Translated_169_residues
MQHLPVGVATIAGIQAGEGKLYLCAIKDACGNRIVGYSIDSRMKARRAVTALNNALARRGDVTGCVVHSDRGSQFSSRKF
LNVLTTHDLIGSMGKVGACGDNAAMESFFSLLQKNVLDRQAWATRQELRTAIVTWIERTYHRRRRQAALGRLTPIEYETI
MNTPAAPAA
>Mature_169_residues
MQHLPVGVATIAGIQAGEGKLYLCAIKDACGNRIVGYSIDSRMKARRAVTALNNALARRGDVTGCVVHSDRGSQFSSRKF
LNVLTTHDLIGSMGKVGACGDNAAMESFFSLLQKNVLDRQAWATRQELRTAIVTWIERTYHRRRRQAALGRLTPIEYETI
MNTPAAPAA

Specific function: Involved in the transposition of the insertion sequence IS3 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1788405, Length=149, Percent_Identity=36.241610738255, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1787263, Length=149, Percent_Identity=36.241610738255, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1786753, Length=149, Percent_Identity=36.241610738255, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1786570, Length=149, Percent_Identity=36.241610738255, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1786490, Length=149, Percent_Identity=36.241610738255, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1789981, Length=145, Percent_Identity=33.1034482758621, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 18504; Mature: 18504

Theoretical pI: Translated: 10.50; Mature: 10.50

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHLPVGVATIAGIQAGEGKLYLCAIKDACGNRIVGYSIDSRMKARRAVTALNNALARRG
CCCCCCHHHHHHCCCCCCCCEEEEEECCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCC
DVTGCVVHSDRGSQFSSRKFLNVLTTHDLIGSMGKVGACGDNAAMESFFSLLQKNVLDRQ
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AWATRQELRTAIVTWIERTYHRRRRQAALGRLTPIEYETIMNTPAAPAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCC
>Mature Secondary Structure
MQHLPVGVATIAGIQAGEGKLYLCAIKDACGNRIVGYSIDSRMKARRAVTALNNALARRG
CCCCCCHHHHHHCCCCCCCCEEEEEECCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCC
DVTGCVVHSDRGSQFSSRKFLNVLTTHDLIGSMGKVGACGDNAAMESFFSLLQKNVLDRQ
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AWATRQELRTAIVTWIERTYHRRRRQAALGRLTPIEYETIMNTPAAPAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987844; 9278503 [H]