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The map label for this gene is gacS [H]

Identifier: 75674318

GI number: 75674318

Start: 140139

End: 142403

Strand: Direct

Name: gacS [H]

Synonym: Nwi_0119

Alternate gene names: 75674318

Gene position: 140139-142403 (Clockwise)

Preceding gene: 75674317

Following gene: 75674326

Centisome position: 4.12

GC content: 63.84

Gene sequence:

>2265_bases
ATGACCGCGCGATTATTCTGGACGAAGGCACGCCTGCGCGCGCGCAGGCTGATCCGGCGGCATCCCCGTCTCAACGTCGC
CTTTCGCACCTTCATGGCCTTCTCAATCGCGTTCGGCGGCGCCTATGGCTTCATCGCCGGAAGCCTGAGCGCCGACTACG
ATCCTCATGCGTTCGCAATCGGCATCAGCTTCCTGTTCGCGCTCGCCTGTGTCGCGCTGGCTATCCTGAGCATGCGCTTG
CGCCGAATGCGCAAGAAGATGCACAAGATTGCTCTCCATAATGAAGCGCTCGTTGACCGCAACTGGGAGCTGAAGGAAGC
GGAAGAGCGCGCGCGCCACCTTTTCGAGTCACAGAGCGATCTGATCGTGCTGCGCGACGGAGGCGGCGTCATCACCTCGG
TGAACGAGGCCTATTGCATCCTGGCCCAGCGATCGCGAACGGAGCTGCTTGGCAGCCGGTTCGCGCTTCCCGTTCTCGAG
CAAGGCGAGAGCGCGATCGAATCGAACGGCACGCGCGTTTACGACCAGCGTGTCGAAACCGCACGGGGTCCGCGCTGGAT
CGCATGGCGCGAAGCCTTGATGCGGATCGACGCCGACCATCCGGCGGAACTGCAATGCGTCGGTCGCGACGTTACCGATC
GAACCGACTCCGAGCGCGCGCTCGCCGAAGCACGGGATCGCGCGAATGAAGCCAGCCAGGCCAAATCGCGCTTCCTCGCG
ATGACGTCGCACGAGTTTCGCACCCCGCTCAACGGCATCATCGGCATGAGCGGGTTGCTGCTCGAAACCTTGCTCACGCC
CGAACAAACGACCTATGTCAAGGCGGTGAAGGCATCCGGCGACGCATTGTTATCGTTGATCGAGGACCTGCTCGACTATT
CCAGGATCGAGGCCGGAAAGGTCGAGCTCGAATGCCGGCCCTTTGTTCTGTCCGACCTGATCGAGGACATAACCGAACTG
CTGGCGCCGCGCGCGCAGGCCAGACAGCTTGAAATCGCCTCCTATGTCGATGAAACGCTGCCAGCCGAGGTAATCGGAGA
CGCCGCGCGCTTGCGGCAGGTGCTGCTCAATCTTGCAGGCAACGCCATCAAGTTTACCTCGTCGGGCGGCGTTGCGCTGA
TCGTCGTTCCCGGCATCCGGCCGGGCGACATCCGCTTCATGGTCCGTGACACGGGAATCGGCATTGCACCGGATGCGCAA
GCCCGGATTTTCCGTGAATTCGAGCAGGCCGACGATCAGATCATTCGGAGCTACGGCGGATCTGGACTGGGGCTGAGCAT
CAGCGATCGCATCGTCAAACGGATGGGTGGGCGAATCACGCTGCAGAGCAAGCCGGACTCCGGTTCGACATTCGAGGTTT
TGGTCCCTCTTCCCGCTCAAGATCGCGCGCCGGAGCGAAAGCGCTTTACCGCGCCGGATCTGACAGGACAGCCGGTCATG
CTCGTGGCACCCCAGGCCATCGAGACATCGCTGCTCGCCCGGCGGCTGGAGCGATGGGGGGCGGAGATCTGCACGGCATC
GGACCTGACGGTGGCTCGTGCTCTGCTTCCGGAGCGCGCGTGGCACGCCGTTGTTGTCGATCATGCGATGGGCGGCGGTG
CGATCTGGCGATTCGGTCAGGCTGCCCGCCAGCATACCGAGCGGCGGATTATCATGGTCACGCCATCGACAAGGCACGAC
CTTCAGGTTGCCGGTGCGGCCGCGTTCACGGGCTATCTTGTCAAGCCGTTGCGAACCGCGTCGATCGCCGCGCGATTGTC
CGCGCCGGCCGACGACCCGACCTCGCTTCGCACCGCCGGCGGCCAGCCTGATTGCGGCAGGCCAGAGACATCCAGGACAC
CGGCCTCCACTTCCGGGCCAAGCCTTTCTATTCTCATCGCCGAGGACAACGAGATCAACGCGCTATTGACGCGCTCGCTC
CTGACCCGTCTCGGACATGCCGTGACCGTCGCCACCAACGGAGAGGAGGCGCTGAAATCGTGGGACGCGGCGCTTGCCGC
AGGCGCGCCCTACGATCTCGTTCTGATGGACGTCCAGATGCCGCATCTCGACGGCATCGAGGCCACCAGGAAAATTCGCG
AACGGGAATCCGTCCGGTCCGGCCGCCGCACGACCATCCTGGCGCTGACCGCCAACGCGCTGATCGACGATCGTCACGCG
TGTTTCGAGGCCGGCATGGATGGCTTTCTCGTCAAGCCGCTCGATCGCGAGAGACTGGCCGACGCGCTTGCGGGATTGAC
GGCCGCCCGTCATGTCCCGGCCTGA

Upstream 100 bases:

>100_bases
ACGTCATGCCTTCATGCTTGAGGGGGTGCATCCACCGCGCGCGCCTTCGTCGGCGGCAACCCGCCTCCGACGGACGCTGC
AAGGATGTGCGGGGAGATCG

Downstream 100 bases:

>100_bases
GGTACTGTAGCGTCTTCGAGCGAAGCGAAATCCGGTTCGCGTGAAGAAACCGCTCACGCAGGGTATTCCATTTCCGGGAT
CATGCTCTAAAGCCGCCTCA

Product: Signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 754; Mature: 753

Protein sequence:

>754_residues
MTARLFWTKARLRARRLIRRHPRLNVAFRTFMAFSIAFGGAYGFIAGSLSADYDPHAFAIGISFLFALACVALAILSMRL
RRMRKKMHKIALHNEALVDRNWELKEAEERARHLFESQSDLIVLRDGGGVITSVNEAYCILAQRSRTELLGSRFALPVLE
QGESAIESNGTRVYDQRVETARGPRWIAWREALMRIDADHPAELQCVGRDVTDRTDSERALAEARDRANEASQAKSRFLA
MTSHEFRTPLNGIIGMSGLLLETLLTPEQTTYVKAVKASGDALLSLIEDLLDYSRIEAGKVELECRPFVLSDLIEDITEL
LAPRAQARQLEIASYVDETLPAEVIGDAARLRQVLLNLAGNAIKFTSSGGVALIVVPGIRPGDIRFMVRDTGIGIAPDAQ
ARIFREFEQADDQIIRSYGGSGLGLSISDRIVKRMGGRITLQSKPDSGSTFEVLVPLPAQDRAPERKRFTAPDLTGQPVM
LVAPQAIETSLLARRLERWGAEICTASDLTVARALLPERAWHAVVVDHAMGGGAIWRFGQAARQHTERRIIMVTPSTRHD
LQVAGAAAFTGYLVKPLRTASIAARLSAPADDPTSLRTAGGQPDCGRPETSRTPASTSGPSLSILIAEDNEINALLTRSL
LTRLGHAVTVATNGEEALKSWDAALAAGAPYDLVLMDVQMPHLDGIEATRKIRERESVRSGRRTTILALTANALIDDRHA
CFEAGMDGFLVKPLDRERLADALAGLTAARHVPA

Sequences:

>Translated_754_residues
MTARLFWTKARLRARRLIRRHPRLNVAFRTFMAFSIAFGGAYGFIAGSLSADYDPHAFAIGISFLFALACVALAILSMRL
RRMRKKMHKIALHNEALVDRNWELKEAEERARHLFESQSDLIVLRDGGGVITSVNEAYCILAQRSRTELLGSRFALPVLE
QGESAIESNGTRVYDQRVETARGPRWIAWREALMRIDADHPAELQCVGRDVTDRTDSERALAEARDRANEASQAKSRFLA
MTSHEFRTPLNGIIGMSGLLLETLLTPEQTTYVKAVKASGDALLSLIEDLLDYSRIEAGKVELECRPFVLSDLIEDITEL
LAPRAQARQLEIASYVDETLPAEVIGDAARLRQVLLNLAGNAIKFTSSGGVALIVVPGIRPGDIRFMVRDTGIGIAPDAQ
ARIFREFEQADDQIIRSYGGSGLGLSISDRIVKRMGGRITLQSKPDSGSTFEVLVPLPAQDRAPERKRFTAPDLTGQPVM
LVAPQAIETSLLARRLERWGAEICTASDLTVARALLPERAWHAVVVDHAMGGGAIWRFGQAARQHTERRIIMVTPSTRHD
LQVAGAAAFTGYLVKPLRTASIAARLSAPADDPTSLRTAGGQPDCGRPETSRTPASTSGPSLSILIAEDNEINALLTRSL
LTRLGHAVTVATNGEEALKSWDAALAAGAPYDLVLMDVQMPHLDGIEATRKIRERESVRSGRRTTILALTANALIDDRHA
CFEAGMDGFLVKPLDRERLADALAGLTAARHVPA
>Mature_753_residues
TARLFWTKARLRARRLIRRHPRLNVAFRTFMAFSIAFGGAYGFIAGSLSADYDPHAFAIGISFLFALACVALAILSMRLR
RMRKKMHKIALHNEALVDRNWELKEAEERARHLFESQSDLIVLRDGGGVITSVNEAYCILAQRSRTELLGSRFALPVLEQ
GESAIESNGTRVYDQRVETARGPRWIAWREALMRIDADHPAELQCVGRDVTDRTDSERALAEARDRANEASQAKSRFLAM
TSHEFRTPLNGIIGMSGLLLETLLTPEQTTYVKAVKASGDALLSLIEDLLDYSRIEAGKVELECRPFVLSDLIEDITELL
APRAQARQLEIASYVDETLPAEVIGDAARLRQVLLNLAGNAIKFTSSGGVALIVVPGIRPGDIRFMVRDTGIGIAPDAQA
RIFREFEQADDQIIRSYGGSGLGLSISDRIVKRMGGRITLQSKPDSGSTFEVLVPLPAQDRAPERKRFTAPDLTGQPVML
VAPQAIETSLLARRLERWGAEICTASDLTVARALLPERAWHAVVVDHAMGGGAIWRFGQAARQHTERRIIMVTPSTRHDL
QVAGAAAFTGYLVKPLRTASIAARLSAPADDPTSLRTAGGQPDCGRPETSRTPASTSGPSLSILIAEDNEINALLTRSLL
TRLGHAVTVATNGEEALKSWDAALAAGAPYDLVLMDVQMPHLDGIEATRKIRERESVRSGRRTTILALTANALIDDRHAC
FEAGMDGFLVKPLDRERLADALAGLTAARHVPA

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=538, Percent_Identity=32.5278810408922, Blast_Score=253, Evalue=3e-68,
Organism=Escherichia coli, GI145693157, Length=544, Percent_Identity=31.8014705882353, Blast_Score=221, Evalue=9e-59,
Organism=Escherichia coli, GI48994928, Length=525, Percent_Identity=29.7142857142857, Blast_Score=177, Evalue=3e-45,
Organism=Escherichia coli, GI87081816, Length=289, Percent_Identity=35.2941176470588, Blast_Score=159, Evalue=7e-40,
Organism=Escherichia coli, GI1788713, Length=293, Percent_Identity=31.740614334471, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1788549, Length=368, Percent_Identity=25.5434782608696, Blast_Score=94, Evalue=5e-20,
Organism=Escherichia coli, GI1788393, Length=230, Percent_Identity=30.4347826086957, Blast_Score=91, Evalue=4e-19,
Organism=Escherichia coli, GI1786912, Length=250, Percent_Identity=29.6, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI87082128, Length=261, Percent_Identity=30.2681992337165, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1790436, Length=248, Percent_Identity=28.6290322580645, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1786600, Length=382, Percent_Identity=28.2722513089005, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1789808, Length=227, Percent_Identity=27.3127753303965, Blast_Score=73, Evalue=7e-14,
Organism=Escherichia coli, GI1787894, Length=245, Percent_Identity=29.7959183673469, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1786783, Length=241, Percent_Identity=24.4813278008299, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1790346, Length=225, Percent_Identity=25.7777777777778, Blast_Score=63, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=130, Percent_Identity=36.1538461538462, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 82512; Mature: 82380

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS50112 PAS ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARLFWTKARLRARRLIRRHPRLNVAFRTFMAFSIAFGGAYGFIAGSLSADYDPHAFAI
CCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHH
GISFLFALACVALAILSMRLRRMRKKMHKIALHNEALVDRNWELKEAEERARHLFESQSD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHCCCCC
LIVLRDGGGVITSVNEAYCILAQRSRTELLGSRFALPVLEQGESAIESNGTRVYDQRVET
EEEEECCCCEEEECCCEEEEEEHHHHHHHHHHHCCCHHHHCCCHHHHCCCCEEHHHHHHH
ARGPRWIAWREALMRIDADHPAELQCVGRDVTDRTDSERALAEARDRANEASQAKSRFLA
CCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MTSHEFRTPLNGIIGMSGLLLETLLTPEQTTYVKAVKASGDALLSLIEDLLDYSRIEAGK
CCCHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHEECCCHHHHHHHHHHHHHHHHCCCCC
VELECRPFVLSDLIEDITELLAPRAQARQLEIASYVDETLPAEVIGDAARLRQVLLNLAG
EEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHCC
NAIKFTSSGGVALIVVPGIRPGDIRFMVRDTGIGIAPDAQARIFREFEQADDQIIRSYGG
CEEEEECCCCEEEEEECCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
SGLGLSISDRIVKRMGGRITLQSKPDSGSTFEVLVPLPAQDRAPERKRFTAPDLTGQPVM
CCCCCCHHHHHHHHCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCEE
LVAPQAIETSLLARRLERWGAEICTASDLTVARALLPERAWHAVVVDHAMGGGAIWRFGQ
EECCHHHHHHHHHHHHHHHCHHHCCCCHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHH
AARQHTERRIIMVTPSTRHDLQVAGAAAFTGYLVKPLRTASIAARLSAPADDPTSLRTAG
HHHHHCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
GQPDCGRPETSRTPASTSGPSLSILIAEDNEINALLTRSLLTRLGHAVTVATNGEEALKS
CCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHH
WDAALAAGAPYDLVLMDVQMPHLDGIEATRKIRERESVRSGRRTTILALTANALIDDRHA
HHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECHHHCCHHH
CFEAGMDGFLVKPLDRERLADALAGLTAARHVPA
HHHCCCCCEEECCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TARLFWTKARLRARRLIRRHPRLNVAFRTFMAFSIAFGGAYGFIAGSLSADYDPHAFAI
CCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHH
GISFLFALACVALAILSMRLRRMRKKMHKIALHNEALVDRNWELKEAEERARHLFESQSD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHCCCCC
LIVLRDGGGVITSVNEAYCILAQRSRTELLGSRFALPVLEQGESAIESNGTRVYDQRVET
EEEEECCCCEEEECCCEEEEEEHHHHHHHHHHHCCCHHHHCCCHHHHCCCCEEHHHHHHH
ARGPRWIAWREALMRIDADHPAELQCVGRDVTDRTDSERALAEARDRANEASQAKSRFLA
CCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MTSHEFRTPLNGIIGMSGLLLETLLTPEQTTYVKAVKASGDALLSLIEDLLDYSRIEAGK
CCCHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHEECCCHHHHHHHHHHHHHHHHCCCCC
VELECRPFVLSDLIEDITELLAPRAQARQLEIASYVDETLPAEVIGDAARLRQVLLNLAG
EEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHCC
NAIKFTSSGGVALIVVPGIRPGDIRFMVRDTGIGIAPDAQARIFREFEQADDQIIRSYGG
CEEEEECCCCEEEEEECCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
SGLGLSISDRIVKRMGGRITLQSKPDSGSTFEVLVPLPAQDRAPERKRFTAPDLTGQPVM
CCCCCCHHHHHHHHCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCEE
LVAPQAIETSLLARRLERWGAEICTASDLTVARALLPERAWHAVVVDHAMGGGAIWRFGQ
EECCHHHHHHHHHHHHHHHCHHHCCCCHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHH
AARQHTERRIIMVTPSTRHDLQVAGAAAFTGYLVKPLRTASIAARLSAPADDPTSLRTAG
HHHHHCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
GQPDCGRPETSRTPASTSGPSLSILIAEDNEINALLTRSLLTRLGHAVTVATNGEEALKS
CCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHH
WDAALAAGAPYDLVLMDVQMPHLDGIEATRKIRERESVRSGRRTTILALTANALIDDRHA
HHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECHHHCCHHH
CFEAGMDGFLVKPLDRERLADALAGLTAARHVPA
HHHCCCCCEEECCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]