Click here to switch to the map view.

The map label for this gene is mltA [H]

Identifier: 75674307

GI number: 75674307

Start: 127167

End: 128675

Strand: Direct

Name: mltA [H]

Synonym: Nwi_0108

Alternate gene names: 75674307

Gene position: 127167-128675 (Clockwise)

Preceding gene: 75674306

Following gene: 75674308

Centisome position: 3.74

GC content: 61.7

Gene sequence:

>1509_bases
TTGGCACCGGAAGCGCCAGCTGAGCTTTATCGGGTCAGAAGGTTCGCGGTCGGATTATGCATCGCCGCCGCGACTGCGCC
TGTCACCGTTCAGGCTCAGGGCAGCGGCCATCGTTCCGTTGGGCCGCTCGAGATCACCGGCGCCCAATATTCGCCGGTTG
CCTTTGCCGATATCGCCGGCTGGAATACCGACAATCATCTCGAGGCATTCAAGGCTTTTCGCGCCAGTTGCCGCGGCGTA
ACCGCGCGGCGCGTCTCGGCCGTCAGCCACAAGGCGCTCGGCGCATCCTTGCGGGAGCCGTGCCGCGTGGCGAAGCCGAT
GAATATCTCGGACAATGCCAAAGCGAGAGCTTTCTTTGAGGACCATTTCCGGGCCGTGCGCATTTCACGTCTCGGCGAAA
CGGACGGCTTCGTCACCGGCTATTACGAGCCGATCATCGACGGATCGCGTACCAGGACCGATGTCTACACCGTGCCGGTC
TACCGCCGTCCTTCGAACCTTTTCGTCCGGGGATTCTCGCAAAGCGCGCCGAGCCTGCCTAACAGCGGGCCGGTGTTTCG
CAAGATTGGCCGCAGAAAGCTGGTTCCCTACTACGATCGCGCGGAAATCGAGGACGGCGCGATCGAAGGCCGCGGCCTGG
AAATCTGCTGGCTGAAGAGCCAGACCGATTTGCTCTTCACGCAAATCCAGGGCTCGGCGCGGGTTCGTCTCGAGGACGGC
TCGACCATACGCATCAACTATGATTCCTATAACGGATATCCATATACCGCGGTGGGTCGTATCCTGATCGAACGCGACAT
CATCCCGAAGGATCAGATGTCGATGCAGCGCATCAGGCAATGGATGGCCGACAACCCCGATGGCGCGAATGAATTGCGCC
GGCAGAACCGTTCCTATGTGTTTTTTCATGAGGTGAAACTCTCCGACAATGACGAGGCCATCGGAGCACAGGGCCTGCCG
TTGACGCCGGGCAGGTCGATTGCGGTCGACAAATCACTGCATGTTTACGGTACGCCTTTCTTTATCGAAGGCGAGCTGCC
GATCGAATCCGCCCAGGCGAAAACGCCGTTCCATCGATTGATGATCGCGCAGGATACAGGGTCCGCCATTGTTGGTCCGG
CCCGCGCGGACCTTTACTTCGGCGCAGGCTCGGAGGCAGGGCGGGTGTCAGGCCGTCTGCGCCACAACATGAACTTCGTC
ATGCTTGTCCCGAACAGCCTTGATCCGGTAGCCCGCGCCAGAACCATGCCGCTTCCGGAAACACGACCTTCGGCAACAAT
CGCAAGGCTGTTTCCGCCGAGCGATCAGAAGCCTGGCAGCACCCCGCCAGCGTCCGGGCTTTCTTCGACCCCCGCGAGAG
AGCAGACCGGGGCCGCCGCTGTGCCGACAGCGAAAGCCGCGTCATCGGAAGCCGCTCCCCTGCCATCGGCGCGGCCCGGG
GTCAGATCAAGTCGGAAACCGCGATATCGCAAGCATCAGCGCCGCCTCTGGCATCGCCGGGCGCGATGA

Upstream 100 bases:

>100_bases
GGAGCCATCGTCGACGGAAACCCGGAGAAGATTTCCGATATTACCGATGTTTGGACGTTTGCCCGCGATATTTCCTCGCG
CGACCCGAACTGGAAACTGG

Downstream 100 bases:

>100_bases
AACGCTTCTCGCCCGACGCATCCGCGCGACCGCGCCGCCGGCGCGTCCTTACCGACGAGGAACGCCGGTTGTGGGAAAGC
GTCGCGGGGCAAGCCAAGCC

Product: membrane-bound lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase A [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MAPEAPAELYRVRRFAVGLCIAAATAPVTVQAQGSGHRSVGPLEITGAQYSPVAFADIAGWNTDNHLEAFKAFRASCRGV
TARRVSAVSHKALGASLREPCRVAKPMNISDNAKARAFFEDHFRAVRISRLGETDGFVTGYYEPIIDGSRTRTDVYTVPV
YRRPSNLFVRGFSQSAPSLPNSGPVFRKIGRRKLVPYYDRAEIEDGAIEGRGLEICWLKSQTDLLFTQIQGSARVRLEDG
STIRINYDSYNGYPYTAVGRILIERDIIPKDQMSMQRIRQWMADNPDGANELRRQNRSYVFFHEVKLSDNDEAIGAQGLP
LTPGRSIAVDKSLHVYGTPFFIEGELPIESAQAKTPFHRLMIAQDTGSAIVGPARADLYFGAGSEAGRVSGRLRHNMNFV
MLVPNSLDPVARARTMPLPETRPSATIARLFPPSDQKPGSTPPASGLSSTPAREQTGAAAVPTAKAASSEAAPLPSARPG
VRSSRKPRYRKHQRRLWHRRAR

Sequences:

>Translated_502_residues
MAPEAPAELYRVRRFAVGLCIAAATAPVTVQAQGSGHRSVGPLEITGAQYSPVAFADIAGWNTDNHLEAFKAFRASCRGV
TARRVSAVSHKALGASLREPCRVAKPMNISDNAKARAFFEDHFRAVRISRLGETDGFVTGYYEPIIDGSRTRTDVYTVPV
YRRPSNLFVRGFSQSAPSLPNSGPVFRKIGRRKLVPYYDRAEIEDGAIEGRGLEICWLKSQTDLLFTQIQGSARVRLEDG
STIRINYDSYNGYPYTAVGRILIERDIIPKDQMSMQRIRQWMADNPDGANELRRQNRSYVFFHEVKLSDNDEAIGAQGLP
LTPGRSIAVDKSLHVYGTPFFIEGELPIESAQAKTPFHRLMIAQDTGSAIVGPARADLYFGAGSEAGRVSGRLRHNMNFV
MLVPNSLDPVARARTMPLPETRPSATIARLFPPSDQKPGSTPPASGLSSTPAREQTGAAAVPTAKAASSEAAPLPSARPG
VRSSRKPRYRKHQRRLWHRRAR
>Mature_501_residues
APEAPAELYRVRRFAVGLCIAAATAPVTVQAQGSGHRSVGPLEITGAQYSPVAFADIAGWNTDNHLEAFKAFRASCRGVT
ARRVSAVSHKALGASLREPCRVAKPMNISDNAKARAFFEDHFRAVRISRLGETDGFVTGYYEPIIDGSRTRTDVYTVPVY
RRPSNLFVRGFSQSAPSLPNSGPVFRKIGRRKLVPYYDRAEIEDGAIEGRGLEICWLKSQTDLLFTQIQGSARVRLEDGS
TIRINYDSYNGYPYTAVGRILIERDIIPKDQMSMQRIRQWMADNPDGANELRRQNRSYVFFHEVKLSDNDEAIGAQGLPL
TPGRSIAVDKSLHVYGTPFFIEGELPIESAQAKTPFHRLMIAQDTGSAIVGPARADLYFGAGSEAGRVSGRLRHNMNFVM
LVPNSLDPVARARTMPLPETRPSATIARLFPPSDQKPGSTPPASGLSSTPAREQTGAAAVPTAKAASSEAAPLPSARPGV
RSSRKPRYRKHQRRLWHRRAR

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division. Degrades murein glycan strands and insoluble, high-molecular weight murein sacculi [H]

COG id: COG2821

COG function: function code M; Membrane-bound lytic murein transglycosylase

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789179, Length=260, Percent_Identity=31.5384615384615, Blast_Score=106, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010611
- InterPro:   IPR014733
- InterPro:   IPR005300 [H]

Pfam domain/function: PF06725 3D; PF03562 MltA [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 55227; Mature: 55096

Theoretical pI: Translated: 10.56; Mature: 10.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPEAPAELYRVRRFAVGLCIAAATAPVTVQAQGSGHRSVGPLEITGAQYSPVAFADIAG
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCEEEEEECC
WNTDNHLEAFKAFRASCRGVTARRVSAVSHKALGASLREPCRVAKPMNISDNAKARAFFE
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
DHFRAVRISRLGETDGFVTGYYEPIIDGSRTRTDVYTVPVYRRPSNLFVRGFSQSAPSLP
HHHHEEEHHHCCCCCCEEEEEEHHHCCCCCCCCEEEEEEEEECCCCEEEEECCCCCCCCC
NSGPVFRKIGRRKLVPYYDRAEIEDGAIEGRGLEICWLKSQTDLLFTQIQGSARVRLEDG
CCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCEEEEEECC
STIRINYDSYNGYPYTAVGRILIERDIIPKDQMSMQRIRQWMADNPDGANELRRQNRSYV
CEEEEEECCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEE
FFHEVKLSDNDEAIGAQGLPLTPGRSIAVDKSLHVYGTPFFIEGELPIESAQAKTPFHRL
EEEEEEECCCCCCCCCCCCCCCCCCCEEECCCEEEEECCEEEECCCCCCCCCCCCCCEEE
MIAQDTGSAIVGPARADLYFGAGSEAGRVSGRLRHNMNFVMLVPNSLDPVARARTMPLPE
EEEECCCCEEECCCCCEEEECCCCCCCHHCHHHCCCCCEEEECCCCCCHHHHHCCCCCCC
TRPSATIARLFPPSDQKPGSTPPASGLSSTPAREQTGAAAVPTAKAASSEAAPLPSARPG
CCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCC
VRSSRKPRYRKHQRRLWHRRAR
CCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
APEAPAELYRVRRFAVGLCIAAATAPVTVQAQGSGHRSVGPLEITGAQYSPVAFADIAG
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCEEEEEECC
WNTDNHLEAFKAFRASCRGVTARRVSAVSHKALGASLREPCRVAKPMNISDNAKARAFFE
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
DHFRAVRISRLGETDGFVTGYYEPIIDGSRTRTDVYTVPVYRRPSNLFVRGFSQSAPSLP
HHHHEEEHHHCCCCCCEEEEEEHHHCCCCCCCCEEEEEEEEECCCCEEEEECCCCCCCCC
NSGPVFRKIGRRKLVPYYDRAEIEDGAIEGRGLEICWLKSQTDLLFTQIQGSARVRLEDG
CCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCEEEEEECC
STIRINYDSYNGYPYTAVGRILIERDIIPKDQMSMQRIRQWMADNPDGANELRRQNRSYV
CEEEEEECCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEE
FFHEVKLSDNDEAIGAQGLPLTPGRSIAVDKSLHVYGTPFFIEGELPIESAQAKTPFHRL
EEEEEEECCCCCCCCCCCCCCCCCCCEEECCCEEEEECCEEEECCCCCCCCCCCCCCEEE
MIAQDTGSAIVGPARADLYFGAGSEAGRVSGRLRHNMNFVMLVPNSLDPVARARTMPLPE
EEEECCCCEEECCCCCEEEECCCCCCCHHCHHHCCCCCEEEECCCCCCHHHHHCCCCCCC
TRPSATIARLFPPSDQKPGSTPPASGLSSTPAREQTGAAAVPTAKAASSEAAPLPSARPG
CCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCC
VRSSRKPRYRKHQRRLWHRRAR
CCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]