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The map label for this gene is dut

Identifier: 75674243

GI number: 75674243

Start: 49555

End: 50013

Strand: Direct

Name: dut

Synonym: Nwi_0044

Alternate gene names: 75674243

Gene position: 49555-50013 (Clockwise)

Preceding gene: 75674242

Following gene: 75674244

Centisome position: 1.46

GC content: 70.15

Gene sequence:

>459_bases
ATGAGCGATCCGATCAAGGTCGATGTCCGGCAACTGCCGCATGCCGAGGGCCTTCCGCTGCCGTCCTACCAAAGCACCCA
GGCGGCCGGTCTTGACCTCATCGCCGCGATTGGCGAACAGGCGCCGCTGGTTCTCGCGGCAGGCCGGCGGGCCATGGTGC
CGACCGGGCTCGTCATCGCGCTGCCCGATGGATACGAGGCGCAGGTGCGGCCGCGCTCCGGCCTCGCCGCACGGCATGGC
GTGACCGTGCTGAATTCGCCCGGCACCGTGGATGCCGACTATCGCGGCGAGATCAACGTGCTTCTTGTGAACCTCGGCAG
CGAGGCCTTCACGATCCGCCGCGGCGAGCGTATCGCGCAGATGATCGTCGCGCCGGTGACTCGCGTGGAACTGGTGCGCG
CGGCGGCGCTGCCGGCGACGCCACGCGGCAGCGGCGGATTCGGCTCGACCGGGCGTTAG

Upstream 100 bases:

>100_bases
ACGGAGAGATCAGGACCGAGTCCTTCCCCGTCATGACCAAGGAAGAGGTGGCCGCCATGCTGATCGAACGCATCATCTCA
GCCCTGCCGGGTCCGGCGGC

Downstream 100 bases:

>100_bases
AGCCTTTTCGTTTCCGATTGAATCGGAAGCGGAGCTTCAGACCGGCCGTGACGCGTTTTCGTCACACGAACAGATCACCC
GCTCCGGTCGCGCAAAAACC

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 152; Mature: 151

Protein sequence:

>152_residues
MSDPIKVDVRQLPHAEGLPLPSYQSTQAAGLDLIAAIGEQAPLVLAAGRRAMVPTGLVIALPDGYEAQVRPRSGLAARHG
VTVLNSPGTVDADYRGEINVLLVNLGSEAFTIRRGERIAQMIVAPVTRVELVRAAALPATPRGSGGFGSTGR

Sequences:

>Translated_152_residues
MSDPIKVDVRQLPHAEGLPLPSYQSTQAAGLDLIAAIGEQAPLVLAAGRRAMVPTGLVIALPDGYEAQVRPRSGLAARHG
VTVLNSPGTVDADYRGEINVLLVNLGSEAFTIRRGERIAQMIVAPVTRVELVRAAALPATPRGSGGFGSTGR
>Mature_151_residues
SDPIKVDVRQLPHAEGLPLPSYQSTQAAGLDLIAAIGEQAPLVLAAGRRAMVPTGLVIALPDGYEAQVRPRSGLAARHGV
TVLNSPGTVDADYRGEINVLLVNLGSEAFTIRRGERIAQMIVAPVTRVELVRAAALPATPRGSGGFGSTGR

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=138, Percent_Identity=43.4782608695652, Blast_Score=108, Evalue=2e-24,
Organism=Homo sapiens, GI4503423, Length=132, Percent_Identity=44.6969696969697, Blast_Score=107, Evalue=4e-24,
Organism=Homo sapiens, GI70906441, Length=132, Percent_Identity=44.6969696969697, Blast_Score=105, Evalue=2e-23,
Organism=Escherichia coli, GI1790071, Length=150, Percent_Identity=43.3333333333333, Blast_Score=116, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI71988561, Length=147, Percent_Identity=41.4965986394558, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6319729, Length=134, Percent_Identity=36.5671641791045, Blast_Score=85, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24583610, Length=150, Percent_Identity=38.6666666666667, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI19921126, Length=131, Percent_Identity=40.4580152671756, Blast_Score=87, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_NITWN (Q3SWM9)

Other databases:

- EMBL:   CP000115
- RefSeq:   YP_316664.1
- ProteinModelPortal:   Q3SWM9
- SMR:   Q3SWM9
- STRING:   Q3SWM9
- GeneID:   3676483
- GenomeReviews:   CP000115_GR
- KEGG:   nwi:Nwi_0044
- NMPDR:   fig|323098.3.peg.41
- eggNOG:   COG0756
- HOGENOM:   HBG436079
- OMA:   LDLRACI
- PhylomeDB:   Q3SWM9
- ProtClustDB:   PRK00601
- BioCyc:   NWIN323098:NWI_0044-MONOMER
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 15826; Mature: 15695

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: BINDING 85-85

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPIKVDVRQLPHAEGLPLPSYQSTQAAGLDLIAAIGEQAPLVLAAGRRAMVPTGLVIA
CCCCEEEHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCEEEECCCCEECCCCEEEE
LPDGYEAQVRPRSGLAARHGVTVLNSPGTVDADYRGEINVLLVNLGSEAFTIRRGERIAQ
CCCCCCEEECCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEEECCCCEEEEECHHHHHH
MIVAPVTRVELVRAAALPATPRGSGGFGSTGR
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDPIKVDVRQLPHAEGLPLPSYQSTQAAGLDLIAAIGEQAPLVLAAGRRAMVPTGLVIA
CCCEEEHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCEEEECCCCEECCCCEEEE
LPDGYEAQVRPRSGLAARHGVTVLNSPGTVDADYRGEINVLLVNLGSEAFTIRRGERIAQ
CCCCCCEEECCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEEECCCCEEEEECHHHHHH
MIVAPVTRVELVRAAALPATPRGSGGFGSTGR
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA