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The map label for this gene is nusA [H]

Identifier: 75674221

GI number: 75674221

Start: 22246

End: 23862

Strand: Direct

Name: nusA [H]

Synonym: Nwi_0022

Alternate gene names: 75674221

Gene position: 22246-23862 (Clockwise)

Preceding gene: 75674220

Following gene: 75674222

Centisome position: 0.65

GC content: 64.13

Gene sequence:

>1617_bases
ATGGCCGCCGTCAGCGCCAATAAGCTCGAACTGCTGCAGATCGCAGACGCGGTGGCGCGCGAAAAGACCATCGACCGCGG
CATCGTGATCGCGGCGATGGAGGACGCCATCGCGAAGGCGGCGCGGGCGCGGTACGGCAGCGAGACTGACGTTCATGCGG
AGATCCACCCGAAGACCGGACAGCTCCAGCTCACCCGCCACATGCTGGTGGTCGAGCAGGTCGAGAATGCCGCGAACCAG
ATCTCGCTGAAGGACGCCCAGCGGGCCAATCCCGGCGCACAGATCGGCGACACCATCGCCGATACGCTGCCGCCGCTGGA
ATATGGCCGCATCTCCGCGCAGTCCGCCAAACAGGTGATCGTGCAGAAGGTGCGCGAGGCCGAACGCGACCGGCAGTATC
AGGAGTTCAAGGATCGCATCGGCGATATCGTCAACGGGATTGTCAAGCGTGTCGAATACGGCAGCGTGATCGTCGACCTC
GGCCGCGGCGAAGCCGTCATTCGCCGCGACGAGATGCTGCCGCGCGAGGTATTCCGCAACGGAGACCGCGTCCGGGCCTA
TATCTTCGACGTGCGCCGCGAAACCCGCGGCCCGCAGATCTTCCTGTCGCGCACCCATCCGCAGTTCATGGTGAAGCTTT
TTACGCAGGAAGTGCCGGAAATCTACGACGGCATCGTCGAGATCAAGGCGGTGGCGCGCGACCCCGGTTCCCGCGCCAAG
ATCGGCGTGGTGTCGCGGGATTCCTCGGTCGATCCGGTCGGCGCCTGCGTCGGCATGCGCGGCTCGCGCGTTCAGGCGGT
CGTCAACGAGTTGCAGGGCGAAAAGATCGACATCATCCCGTGGTCGCCGGATATCGCGACCTTCGTCGTCAACGCGCTGG
CGCCGGCGGAGGTCGCGAAAGTCGTGATCGACGAGGACCGCGAGCGGATCGAGGTCGTGGTCCCCGACACCAACAACCAG
TTGTCGCTCGCGATCGGACGGCGCGGACAGAACGTGCGGCTCGCCTCGCAACTGACGGGCTGGGACATCGACATCCTGAC
CGAGCAGGAGGAATCGGAGCGACGCCAGGCGGACTTCGAGAATGCGACCCGCATGTTCATGGAAACACTCAACGTCGACG
AGGTCGTCGGGCAGTTGCTGGCGTCCGAGGGTTTCACCTCTGTTGAAGAACTGACGCTGGTCGACACGCGGGAAATCGCC
GGCATCGAGGGCTTTGACGACGAAACCGCGACCGAATTGCAGAACCGGGCCCGAGAATATCTGGAGCAGCTCGAGGCCGA
ACTGGAAAACAGGCGCAAGGAGCTCGGCGTGGATGATGCGCTGAAGACCGTGCCCGGCGTGACCTCGAAGATGCTGGTGA
AACTCGGCGAAAACGAAGTCAGGACCATCGAGGATCTGGCCGGCTGCGCCACCGACGATCTGGTCGGCTGGACCGAACGC
AAGGAGGGCGAAGCGGTCAAGCACGCGGGTTATTTCGACGGCATCGAGATCTCGCGGGAGGACGCGGAGGCCATCATCAT
GCAGGCTCGCCTGAGTGTCGGCTGGATCAACGAGGCCGACCTCGCGAAACCGGCGGAGGCTGAGGATATCGCTGCTGAAG
ATCAGCCGGCCGAATGA

Upstream 100 bases:

>100_bases
AGCACCGGGGAAAATCCCGCCGAAGAACACCAAGGAACATCGTCTGGCCGCGGAACGACTGCGGCGCGGCGACATCGATC
CCATCGAAGGAGAATAAGTC

Downstream 100 bases:

>100_bases
AGAAAGCCTTGGAGCCCTTTCGCTTCTGGTGGAATCAGAAGCGAGGCTCCCAGATTTTGATTTGATGCGTTTTCTTCACG
CGAACCGGTGTCCGCTTCGC

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MAAVSANKLELLQIADAVAREKTIDRGIVIAAMEDAIAKAARARYGSETDVHAEIHPKTGQLQLTRHMLVVEQVENAANQ
ISLKDAQRANPGAQIGDTIADTLPPLEYGRISAQSAKQVIVQKVREAERDRQYQEFKDRIGDIVNGIVKRVEYGSVIVDL
GRGEAVIRRDEMLPREVFRNGDRVRAYIFDVRRETRGPQIFLSRTHPQFMVKLFTQEVPEIYDGIVEIKAVARDPGSRAK
IGVVSRDSSVDPVGACVGMRGSRVQAVVNELQGEKIDIIPWSPDIATFVVNALAPAEVAKVVIDEDRERIEVVVPDTNNQ
LSLAIGRRGQNVRLASQLTGWDIDILTEQEESERRQADFENATRMFMETLNVDEVVGQLLASEGFTSVEELTLVDTREIA
GIEGFDDETATELQNRAREYLEQLEAELENRRKELGVDDALKTVPGVTSKMLVKLGENEVRTIEDLAGCATDDLVGWTER
KEGEAVKHAGYFDGIEISREDAEAIIMQARLSVGWINEADLAKPAEAEDIAAEDQPAE

Sequences:

>Translated_538_residues
MAAVSANKLELLQIADAVAREKTIDRGIVIAAMEDAIAKAARARYGSETDVHAEIHPKTGQLQLTRHMLVVEQVENAANQ
ISLKDAQRANPGAQIGDTIADTLPPLEYGRISAQSAKQVIVQKVREAERDRQYQEFKDRIGDIVNGIVKRVEYGSVIVDL
GRGEAVIRRDEMLPREVFRNGDRVRAYIFDVRRETRGPQIFLSRTHPQFMVKLFTQEVPEIYDGIVEIKAVARDPGSRAK
IGVVSRDSSVDPVGACVGMRGSRVQAVVNELQGEKIDIIPWSPDIATFVVNALAPAEVAKVVIDEDRERIEVVVPDTNNQ
LSLAIGRRGQNVRLASQLTGWDIDILTEQEESERRQADFENATRMFMETLNVDEVVGQLLASEGFTSVEELTLVDTREIA
GIEGFDDETATELQNRAREYLEQLEAELENRRKELGVDDALKTVPGVTSKMLVKLGENEVRTIEDLAGCATDDLVGWTER
KEGEAVKHAGYFDGIEISREDAEAIIMQARLSVGWINEADLAKPAEAEDIAAEDQPAE
>Mature_537_residues
AAVSANKLELLQIADAVAREKTIDRGIVIAAMEDAIAKAARARYGSETDVHAEIHPKTGQLQLTRHMLVVEQVENAANQI
SLKDAQRANPGAQIGDTIADTLPPLEYGRISAQSAKQVIVQKVREAERDRQYQEFKDRIGDIVNGIVKRVEYGSVIVDLG
RGEAVIRRDEMLPREVFRNGDRVRAYIFDVRRETRGPQIFLSRTHPQFMVKLFTQEVPEIYDGIVEIKAVARDPGSRAKI
GVVSRDSSVDPVGACVGMRGSRVQAVVNELQGEKIDIIPWSPDIATFVVNALAPAEVAKVVIDEDRERIEVVVPDTNNQL
SLAIGRRGQNVRLASQLTGWDIDILTEQEESERRQADFENATRMFMETLNVDEVVGQLLASEGFTSVEELTLVDTREIAG
IEGFDDETATELQNRAREYLEQLEAELENRRKELGVDDALKTVPGVTSKMLVKLGENEVRTIEDLAGCATDDLVGWTERK
EGEAVKHAGYFDGIEISREDAEAIIMQARLSVGWINEADLAKPAEAEDIAAEDQPAE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=484, Percent_Identity=43.3884297520661, Blast_Score=385, Evalue=1e-108,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 59602; Mature: 59471

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAVSANKLELLQIADAVAREKTIDRGIVIAAMEDAIAKAARARYGSETDVHAEIHPKTG
CCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCCCCCEEEEECCCCC
QLQLTRHMLVVEQVENAANQISLKDAQRANPGAQIGDTIADTLPPLEYGRISAQSAKQVI
CCHHHHHHHHHHHHHHHHHHEEHHHHHCCCCCCHHHHHHHHHCCCCHHCCCCHHHHHHHH
VQKVREAERDRQYQEFKDRIGDIVNGIVKRVEYGSVIVDLGRGEAVIRRDEMLPREVFRN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEHHHHHCCHHHHHCC
GDRVRAYIFDVRRETRGPQIFLSRTHPQFMVKLFTQEVPEIYDGIVEIKAVARDPGSRAK
CCCEEEHHEEHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
IGVVSRDSSVDPVGACVGMRGSRVQAVVNELQGEKIDIIPWSPDIATFVVNALAPAEVAK
EEEEECCCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCHHHHHH
VVIDEDRERIEVVVPDTNNQLSLAIGRRGQNVRLASQLTGWDIDILTEQEESERRQADFE
HHHCCCCCEEEEEECCCCCEEEEEECCCCCCEEEHHHCCCCCEEEECCCHHHHHHHHHHH
NATRMFMETLNVDEVVGQLLASEGFTSVEELTLVDTREIAGIEGFDDETATELQNRAREY
HHHHHHHHHCCHHHHHHHHHHHCCCCCHHHEEEECHHHHCCCCCCCCHHHHHHHHHHHHH
LEQLEAELENRRKELGVDDALKTVPGVTSKMLVKLGENEVRTIEDLAGCATDDLVGWTER
HHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCCHHHCCCCCC
KEGEAVKHAGYFDGIEISREDAEAIIMQARLSVGWINEADLAKPAEAEDIAAEDQPAE
CCCCHHHHCCCCCCCEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCC
>Mature Secondary Structure 
AAVSANKLELLQIADAVAREKTIDRGIVIAAMEDAIAKAARARYGSETDVHAEIHPKTG
CCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCCCCCEEEEECCCCC
QLQLTRHMLVVEQVENAANQISLKDAQRANPGAQIGDTIADTLPPLEYGRISAQSAKQVI
CCHHHHHHHHHHHHHHHHHHEEHHHHHCCCCCCHHHHHHHHHCCCCHHCCCCHHHHHHHH
VQKVREAERDRQYQEFKDRIGDIVNGIVKRVEYGSVIVDLGRGEAVIRRDEMLPREVFRN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEHHHHHCCHHHHHCC
GDRVRAYIFDVRRETRGPQIFLSRTHPQFMVKLFTQEVPEIYDGIVEIKAVARDPGSRAK
CCCEEEHHEEHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
IGVVSRDSSVDPVGACVGMRGSRVQAVVNELQGEKIDIIPWSPDIATFVVNALAPAEVAK
EEEEECCCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCHHHHHH
VVIDEDRERIEVVVPDTNNQLSLAIGRRGQNVRLASQLTGWDIDILTEQEESERRQADFE
HHHCCCCCEEEEEECCCCCEEEEEECCCCCCEEEHHHCCCCCEEEECCCHHHHHHHHHHH
NATRMFMETLNVDEVVGQLLASEGFTSVEELTLVDTREIAGIEGFDDETATELQNRAREY
HHHHHHHHHCCHHHHHHHHHHHCCCCCHHHEEEECHHHHCCCCCCCCHHHHHHHHHHHHH
LEQLEAELENRRKELGVDDALKTVPGVTSKMLVKLGENEVRTIEDLAGCATDDLVGWTER
HHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCCHHHCCCCCC
KEGEAVKHAGYFDGIEISREDAEAIIMQARLSVGWINEADLAKPAEAEDIAAEDQPAE
CCCCHHHHCCCCCCCEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]