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The map label for this gene is mlaE [H]

Identifier: 73542805

GI number: 73542805

Start: 3426739

End: 3427515

Strand: Reverse

Name: mlaE [H]

Synonym: Reut_A3121

Alternate gene names: 73542805

Gene position: 3427515-3426739 (Counterclockwise)

Preceding gene: 73542806

Following gene: 73542804

Centisome position: 90.04

GC content: 63.96

Gene sequence:

>777_bases
ATGACGGGCTTCTTCGCCGCCATCGGCTCGGCGGTGCGGCAAGGTGTCGCCGGCCTCGGCCATGCCGCAGGCATGTTCTT
TACCGTGCTGGGCTTGTCCCCGGCACTGCTGCGCCGCTTCCGTCTGGTGACCGACCAGATCTTCTTCGTCGGCAACCTGT
CACTCGTCATCATCGCGGTGTCGGGCTTGTTCGTTGGCTTCGTGCTGGGGCTGCAGGGCTACTACACGCTGAACCGCTAT
GGTTCCGAGCAGGCGCTCGGGCTGCTCGTAGCGCTTTCGCTGGTGCGCGAGCTGGGGCCGGTCATCACCGCATTGCTGTT
TGCCGGCCGGGCGGGCACGTCCCTGACCGCGGAAATCGGACTGATGAAGGCGGGCGAGCAGTTGACCGCCATGGAAATGA
TGGCCGTCAATCCGCTGCAACGCGTGATTGCGCCGCGTTTCTGGGCAGGTGTGGTGGCTATGCCGGTGCTGGCCGCGATC
TTCAGCGCTGTCGGCGTCCTGGGCGGTTACCTGGTCGGCGTGCAATTGATCGGCGTGGATGCGGGCGCCTTCTGGTCGCA
GATGCAGGCTGGCGTGGATGTGCGTGACGACGTGGTCAACGGCGTCATCAAGAGTTTTATTTTCGGCATTGCCGTGACGT
TCATTGCGCTGTACCAGGGGTTCGAAGCCAAGCCCACGCCGGAAGGCGTGTCGCGGGCCACAACCCGCACCGTGGTGATC
GCGTCGCTGGCCGTGCTTGGGCTGGATTTCCTGCTGACAGCGTTGATGTTCAGTTGA

Upstream 100 bases:

>100_bases
CGTTCGTGCGCCAGTTCGTGCATGCGGAGGCCGACGGCCCCGTGCCTTTCCACTATCCGGGCCCGTCGCTGGTCGAGGAC
TTTGCCGGCGGGGGTGCGCA

Downstream 100 bases:

>100_bases
TGGGCAACGCACCGGGCCAAACGGCAGGACAACGGGTAGAAAGAGATCCCAATGAAAAAAAGTGCACTCGATTTCTGGGT
GGGGCTGTTCGTGGTAGCAG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MTGFFAAIGSAVRQGVAGLGHAAGMFFTVLGLSPALLRRFRLVTDQIFFVGNLSLVIIAVSGLFVGFVLGLQGYYTLNRY
GSEQALGLLVALSLVRELGPVITALLFAGRAGTSLTAEIGLMKAGEQLTAMEMMAVNPLQRVIAPRFWAGVVAMPVLAAI
FSAVGVLGGYLVGVQLIGVDAGAFWSQMQAGVDVRDDVVNGVIKSFIFGIAVTFIALYQGFEAKPTPEGVSRATTRTVVI
ASLAVLGLDFLLTALMFS

Sequences:

>Translated_258_residues
MTGFFAAIGSAVRQGVAGLGHAAGMFFTVLGLSPALLRRFRLVTDQIFFVGNLSLVIIAVSGLFVGFVLGLQGYYTLNRY
GSEQALGLLVALSLVRELGPVITALLFAGRAGTSLTAEIGLMKAGEQLTAMEMMAVNPLQRVIAPRFWAGVVAMPVLAAI
FSAVGVLGGYLVGVQLIGVDAGAFWSQMQAGVDVRDDVVNGVIKSFIFGIAVTFIALYQGFEAKPTPEGVSRATTRTVVI
ASLAVLGLDFLLTALMFS
>Mature_257_residues
TGFFAAIGSAVRQGVAGLGHAAGMFFTVLGLSPALLRRFRLVTDQIFFVGNLSLVIIAVSGLFVGFVLGLQGYYTLNRYG
SEQALGLLVALSLVRELGPVITALLFAGRAGTSLTAEIGLMKAGEQLTAMEMMAVNPLQRVIAPRFWAGVVAMPVLAAIF
SAVGVLGGYLVGVQLIGVDAGAFWSQMQAGVDVRDDVVNGVIKSFIFGIAVTFIALYQGFEAKPTPEGVSRATTRTVVIA
SLAVLGLDFLLTALMFS

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=254, Percent_Identity=59.4488188976378, Blast_Score=290, Evalue=4e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 27027; Mature: 26896

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGFFAAIGSAVRQGVAGLGHAAGMFFTVLGLSPALLRRFRLVTDQIFFVGNLSLVIIAV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGLFVGFVLGLQGYYTLNRYGSEQALGLLVALSLVRELGPVITALLFAGRAGTSLTAEIG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LMKAGEQLTAMEMMAVNPLQRVIAPRFWAGVVAMPVLAAIFSAVGVLGGYLVGVQLIGVD
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
AGAFWSQMQAGVDVRDDVVNGVIKSFIFGIAVTFIALYQGFEAKPTPEGVSRATTRTVVI
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
ASLAVLGLDFLLTALMFS
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TGFFAAIGSAVRQGVAGLGHAAGMFFTVLGLSPALLRRFRLVTDQIFFVGNLSLVIIAV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGLFVGFVLGLQGYYTLNRYGSEQALGLLVALSLVRELGPVITALLFAGRAGTSLTAEIG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LMKAGEQLTAMEMMAVNPLQRVIAPRFWAGVVAMPVLAAIFSAVGVLGGYLVGVQLIGVD
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
AGAFWSQMQAGVDVRDDVVNGVIKSFIFGIAVTFIALYQGFEAKPTPEGVSRATTRTVVI
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
ASLAVLGLDFLLTALMFS
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]