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The map label for this gene is hisH

Identifier: 73542791

GI number: 73542791

Start: 3415188

End: 3415841

Strand: Reverse

Name: hisH

Synonym: Reut_A3107

Alternate gene names: 73542791

Gene position: 3415841-3415188 (Counterclockwise)

Preceding gene: 73542792

Following gene: 73542790

Centisome position: 89.74

GC content: 63.76

Gene sequence:

>654_bases
ATGACTACCATAGCAATTGTGGATTACGGCGTGGGCAACCTGCGCTCGGTGGCGCAGGCCCTGCGGGCCGCGGCACCGGA
GGCGGATGTGCGCGTGGTGGATGCCCCGGAAGGCATTCGGAGCGCGGATCGCGTGGTGCTGCCGGGGCAGGGCGCCATGC
CTGACTGCATGTCGGCGCTGGGCGCTTCGGGGCTGCAGGAGGCGGTGATCGAGGCGGCAGCCAGCAAGCCGATGTTCGGT
GTGTGCGTTGGCGAACAGATGCTGTTCGAGTCCAGCAGCGAAGCCAGGCCCGGCACCGACAAGACGCCTTGCCTGGGGCT
GATGCCGGGTGAAGTCATCCGCTTCGAACTGGACGGGATGACCCAGCCGGACGGCTCGCGCTACAAGGTGCCGCAGATGG
GCTGGAACCGCGTCAAACAGTCCAGGCCCCATCCGCTGTGGGATGGCATCCCGGACGAAAGCTGGTTTTACTTCGTGCAC
AGCTACTTCGTGCGCGCGCAGGATCCGGCCCATATTGCCGGCGAAACGGAGTATGGGGTCGTGTTTACCAGTGCGGTAGC
ACGCGATAATATTTTTGCCACGCAGTTCCACCCCGAAAAAAGCGCAGCCATGGGGTTGCAGCTTTACCGGAACTTCGTGC
ACTGGAATCCCTGA

Upstream 100 bases:

>100_bases
GCATCAATATCGGCACGCGCCTGATGGGGCTGATCCTGTCGGCGCTGGCCGTTGAATTCATTGTGGACGGGCTGAAGACA
TTATTGCCTGTTTTGAAATC

Downstream 100 bases:

>100_bases
AGATCCGATCCGAGCCAGCCAACGCCATTCTGGAATACTTGCCTATTGACCCGGACTGACCGGCCACTGGCCGGGCTGGC
TCTATCTCTCTCACTCAACC

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MTTIAIVDYGVGNLRSVAQALRAAAPEADVRVVDAPEGIRSADRVVLPGQGAMPDCMSALGASGLQEAVIEAAASKPMFG
VCVGEQMLFESSSEARPGTDKTPCLGLMPGEVIRFELDGMTQPDGSRYKVPQMGWNRVKQSRPHPLWDGIPDESWFYFVH
SYFVRAQDPAHIAGETEYGVVFTSAVARDNIFATQFHPEKSAAMGLQLYRNFVHWNP

Sequences:

>Translated_217_residues
MTTIAIVDYGVGNLRSVAQALRAAAPEADVRVVDAPEGIRSADRVVLPGQGAMPDCMSALGASGLQEAVIEAAASKPMFG
VCVGEQMLFESSSEARPGTDKTPCLGLMPGEVIRFELDGMTQPDGSRYKVPQMGWNRVKQSRPHPLWDGIPDESWFYFVH
SYFVRAQDPAHIAGETEYGVVFTSAVARDNIFATQFHPEKSAAMGLQLYRNFVHWNP
>Mature_216_residues
TTIAIVDYGVGNLRSVAQALRAAAPEADVRVVDAPEGIRSADRVVLPGQGAMPDCMSALGASGLQEAVIEAAASKPMFGV
CVGEQMLFESSSEARPGTDKTPCLGLMPGEVIRFELDGMTQPDGSRYKVPQMGWNRVKQSRPHPLWDGIPDESWFYFVHS
YFVRAQDPAHIAGETEYGVVFTSAVARDNIFATQFHPEKSAAMGLQLYRNFVHWNP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=211, Percent_Identity=35.0710900473934, Blast_Score=112, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6319725, Length=225, Percent_Identity=32, Blast_Score=99, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_CUPPJ (Q46WL6)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_297311.1
- ProteinModelPortal:   Q46WL6
- GeneID:   3609698
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A3107
- NMPDR:   fig|264198.3.peg.3547
- HOGENOM:   HBG292341
- OMA:   RPFFGIC
- ProtClustDB:   PRK13146
- BioCyc:   REUT264198:REUT_A3107-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 23661; Mature: 23530

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 82-82 ACT_SITE 197-197 ACT_SITE 199-199

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIAIVDYGVGNLRSVAQALRAAAPEADVRVVDAPEGIRSADRVVLPGQGAMPDCMSAL
CCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEECCCCCCHHHHHHH
GASGLQEAVIEAAASKPMFGVCVGEQMLFESSSEARPGTDKTPCLGLMPGEVIRFELDGM
CCCHHHHHHHHHHHCCCEEEEECCHHHHHCCCCCCCCCCCCCCEEECCCCEEEEEEECCC
TQPDGSRYKVPQMGWNRVKQSRPHPLWDGIPDESWFYFVHSYFVRAQDPAHIAGETEYGV
CCCCCCEEECCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCE
VFTSAVARDNIFATQFHPEKSAAMGLQLYRNFVHWNP
EEEHHHHCCCEEEEEECCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTIAIVDYGVGNLRSVAQALRAAAPEADVRVVDAPEGIRSADRVVLPGQGAMPDCMSAL
CEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEECCCCCCHHHHHHH
GASGLQEAVIEAAASKPMFGVCVGEQMLFESSSEARPGTDKTPCLGLMPGEVIRFELDGM
CCCHHHHHHHHHHHCCCEEEEECCHHHHHCCCCCCCCCCCCCCEEECCCCEEEEEEECCC
TQPDGSRYKVPQMGWNRVKQSRPHPLWDGIPDESWFYFVHSYFVRAQDPAHIAGETEYGV
CCCCCCEEECCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCE
VFTSAVARDNIFATQFHPEKSAAMGLQLYRNFVHWNP
EEEHHHHCCCEEEEEECCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA