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The map label for this gene is ubiF [H]

Identifier: 73542720

GI number: 73542720

Start: 3340431

End: 3341708

Strand: Reverse

Name: ubiF [H]

Synonym: Reut_A3036

Alternate gene names: 73542720

Gene position: 3341708-3340431 (Counterclockwise)

Preceding gene: 73542722

Following gene: 73542719

Centisome position: 87.79

GC content: 69.48

Gene sequence:

>1278_bases
ATGACCCGATCCAACGCGTCCATCCGCAATTCCTCAGCTTTCCAGATCGCCGTTGTCGGCGGCGGCATCGTGGGCAAGTC
CTGCGCGCTGCTGCTGGCGCAGCAGGGCATGAGCGTGGCCCTGGCCGCGCCGCGTCCAACGCGCGGGCGCCCCCCGGCCG
GGCCTGACGACTGGGATAGCCGTATCTATGCGTTTTCCGCGAGTTCGCAGGCGCTGCTCGCGCGCATGCGCGTGTGGGAG
GCGCTCGATCCGTCCCGCATCCAGCCAGTGCGCGATATGCGCGTGTTCGGCGACGTGAGCGCCGCCGAGACCTCGCCGAG
CCTCGATGGCGACCTGCATTTCTCGGCCTACGCCGCGGCCGTGCCGCAGCTCGCGTGGATCGTCGAGTCCGGGCTCGTGG
AGCGCGCGCTCGATACCGCGCTCGGCTTCCAGCACCAGGTCCAGTGGCATGACGACACCGCCGCAGGCTTGGAGCGCGAT
GCCGATGGCGCCACCCTGACGCTTTCCGGTGGTACGAAGATCCGCGCGGCATGTGTGGTCGGGGCCGATGGCGCGCGCTC
CTGGGTGCGCCAGCAGTGCCATATCGGCGTGACCACGCGGCGTTACCGCCAGCTTGGCGTGGTGGCGAACTTCGAATGCG
AGCGGCCGCACCACGACACGGCTTGGCAGTGGTTCCTGGGCGCGCCGGAAAAGCTGATGGCCGACGAGGAGCCGGCCAAC
GGCGAGATCCTCGCCATGCTGCCGCTGCCGGGTTCGCGCGTGTCGATGGTGTGGTCGGCCGACGAGGCGCACGCGCATGA
CCTGCTCGCGCTGTCGCCAGAGGCGCTGGCCAACACAGTAATGCAAGCTGCCTCCGGCGCAGTTGGCAGCCAGTTCGGCA
CGCTGCGGTGTATCACGCCGGCGCAGGGATTTCCGCTGGTGCTGCAGCGCGCGGAGCAGTTTGTGCAGCCGCATCTGGCG
CTGGTGGGCGACGCCGCGCATGTGGTGCATCCGCTTGCGGGGCAGGGCATGAACCTGGGTTTGCGCGATGTTGCGGAACT
CGGCAAGGTCATGGCCGCCAAAGAATCTTTCCGCAGCGAGGGCGACCTGCGCCTGCTGCGCCGCTATGAGCGCGCGCGCG
CCACCGACTTGCTGTCGCTGACCGCCGCGACCGACGGCCTGCACCGCTTGTTCTCGCTGCCCGGCAGCGCGGCGCGCGTG
GTGCGCAACACCGGCATCCGCACCGTAGGGCGGCAGTCGCTGCTCAAGCGCATCCTGATTGAACGGGCGCTCGGCTGA

Upstream 100 bases:

>100_bases
AATAGCAAAACCCATATTCTAGCGGATGGGGGCTCTCGGCCTTGCCCCGCCGCGCCATGAGCCGCCGGGGCCGCGCGGGT
GAGGTGGCTATAATCCAGCC

Downstream 100 bases:

>100_bases
CGCCGGGTGCCCGGACCTTGACTGAATACCTGGAGTCGATATGTTCCAACTCATGCGCCGCCACCCTGCCTGGACCACTG
CCATTGCCGGCGGTCTGGCG

Product: ubiquinone biosynthesis hydroxylase family protein

Products: 2-Octaprenyl-3-Methyl-5-Hydroxy-6-Methoxy-1,4- Benzoquinol [C]

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MTRSNASIRNSSAFQIAVVGGGIVGKSCALLLAQQGMSVALAAPRPTRGRPPAGPDDWDSRIYAFSASSQALLARMRVWE
ALDPSRIQPVRDMRVFGDVSAAETSPSLDGDLHFSAYAAAVPQLAWIVESGLVERALDTALGFQHQVQWHDDTAAGLERD
ADGATLTLSGGTKIRAACVVGADGARSWVRQQCHIGVTTRRYRQLGVVANFECERPHHDTAWQWFLGAPEKLMADEEPAN
GEILAMLPLPGSRVSMVWSADEAHAHDLLALSPEALANTVMQAASGAVGSQFGTLRCITPAQGFPLVLQRAEQFVQPHLA
LVGDAAHVVHPLAGQGMNLGLRDVAELGKVMAAKESFRSEGDLRLLRRYERARATDLLSLTAATDGLHRLFSLPGSAARV
VRNTGIRTVGRQSLLKRILIERALG

Sequences:

>Translated_425_residues
MTRSNASIRNSSAFQIAVVGGGIVGKSCALLLAQQGMSVALAAPRPTRGRPPAGPDDWDSRIYAFSASSQALLARMRVWE
ALDPSRIQPVRDMRVFGDVSAAETSPSLDGDLHFSAYAAAVPQLAWIVESGLVERALDTALGFQHQVQWHDDTAAGLERD
ADGATLTLSGGTKIRAACVVGADGARSWVRQQCHIGVTTRRYRQLGVVANFECERPHHDTAWQWFLGAPEKLMADEEPAN
GEILAMLPLPGSRVSMVWSADEAHAHDLLALSPEALANTVMQAASGAVGSQFGTLRCITPAQGFPLVLQRAEQFVQPHLA
LVGDAAHVVHPLAGQGMNLGLRDVAELGKVMAAKESFRSEGDLRLLRRYERARATDLLSLTAATDGLHRLFSLPGSAARV
VRNTGIRTVGRQSLLKRILIERALG
>Mature_424_residues
TRSNASIRNSSAFQIAVVGGGIVGKSCALLLAQQGMSVALAAPRPTRGRPPAGPDDWDSRIYAFSASSQALLARMRVWEA
LDPSRIQPVRDMRVFGDVSAAETSPSLDGDLHFSAYAAAVPQLAWIVESGLVERALDTALGFQHQVQWHDDTAAGLERDA
DGATLTLSGGTKIRAACVVGADGARSWVRQQCHIGVTTRRYRQLGVVANFECERPHHDTAWQWFLGAPEKLMADEEPANG
EILAMLPLPGSRVSMVWSADEAHAHDLLALSPEALANTVMQAASGAVGSQFGTLRCITPAQGFPLVLQRAEQFVQPHLAL
VGDAAHVVHPLAGQGMNLGLRDVAELGKVMAAKESFRSEGDLRLLRRYERARATDLLSLTAATDGLHRLFSLPGSAARVV
RNTGIRTVGRQSLLKRILIERALG

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=409, Percent_Identity=26.8948655256724, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI32967307, Length=289, Percent_Identity=31.1418685121107, Blast_Score=112, Evalue=6e-25,
Organism=Escherichia coli, GI1786883, Length=421, Percent_Identity=34.4418052256532, Blast_Score=181, Evalue=8e-47,
Organism=Escherichia coli, GI1789273, Length=418, Percent_Identity=32.2966507177034, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1789274, Length=423, Percent_Identity=28.8416075650118, Blast_Score=119, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI25146631, Length=467, Percent_Identity=27.4089935760171, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI19920760, Length=413, Percent_Identity=28.0871670702179, Blast_Score=125, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 45607; Mature: 45475

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRSNASIRNSSAFQIAVVGGGIVGKSCALLLAQQGMSVALAAPRPTRGRPPAGPDDWDS
CCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCC
RIYAFSASSQALLARMRVWEALDPSRIQPVRDMRVFGDVSAAETSPSLDGDLHFSAYAAA
EEEEECCCHHHHHHHHHHHHCCCCHHCCHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHH
VPQLAWIVESGLVERALDTALGFQHQVQWHDDTAAGLERDADGATLTLSGGTKIRAACVV
HHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHCCCCCCCCCEEEECCCCEEEEEEEE
GADGARSWVRQQCHIGVTTRRYRQLGVVANFECERPHHDTAWQWFLGAPEKLMADEEPAN
CCCHHHHHHHHHHCCCCHHHHHHHHCCEEECCCCCCCCCCCHHEECCCCHHHHCCCCCCC
GEILAMLPLPGSRVSMVWSADEAHAHDLLALSPEALANTVMQAASGAVGSQFGTLRCITP
CCEEEEECCCCCCEEEEEECCCCCCCHHEEECHHHHHHHHHHHHCCCCCCCCCEEEEECC
AQGFPLVLQRAEQFVQPHLALVGDAAHVVHPLAGQGMNLGLRDVAELGKVMAAKESFRSE
CCCCHHHHHHHHHHHCCHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCC
GDLRLLRRYERARATDLLSLTAATDGLHRLFSLPGSAARVVRNTGIRTVGRQSLLKRILI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHH
ERALG
HHHCC
>Mature Secondary Structure 
TRSNASIRNSSAFQIAVVGGGIVGKSCALLLAQQGMSVALAAPRPTRGRPPAGPDDWDS
CCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCC
RIYAFSASSQALLARMRVWEALDPSRIQPVRDMRVFGDVSAAETSPSLDGDLHFSAYAAA
EEEEECCCHHHHHHHHHHHHCCCCHHCCHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHH
VPQLAWIVESGLVERALDTALGFQHQVQWHDDTAAGLERDADGATLTLSGGTKIRAACVV
HHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHCCCCCCCCCEEEECCCCEEEEEEEE
GADGARSWVRQQCHIGVTTRRYRQLGVVANFECERPHHDTAWQWFLGAPEKLMADEEPAN
CCCHHHHHHHHHHCCCCHHHHHHHHCCEEECCCCCCCCCCCHHEECCCCHHHHCCCCCCC
GEILAMLPLPGSRVSMVWSADEAHAHDLLALSPEALANTVMQAASGAVGSQFGTLRCITP
CCEEEEECCCCCCEEEEEECCCCCCCHHEEECHHHHHHHHHHHHCCCCCCCCCEEEEECC
AQGFPLVLQRAEQFVQPHLALVGDAAHVVHPLAGQGMNLGLRDVAELGKVMAAKESFRSE
CCCCHHHHHHHHHHHCCHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCC
GDLRLLRRYERARATDLLSLTAATDGLHRLFSLPGSAARVVRNTGIRTVGRQSLLKRILI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHH
ERALG
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: @OCTA02.txt*2-Octaprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol! [C]

Specific reaction: @OCTA02.txt*2-Octaprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol! = 2-Octaprenyl-3-Methyl-5-Hydroxy-6-Methoxy-1,4- Benzoquinol [C]

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]