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The map label for this gene is fliY [H]

Identifier: 73542698

GI number: 73542698

Start: 3317397

End: 3318194

Strand: Reverse

Name: fliY [H]

Synonym: Reut_A3014

Alternate gene names: 73542698

Gene position: 3318194-3317397 (Counterclockwise)

Preceding gene: 73542702

Following gene: 73542697

Centisome position: 87.17

GC content: 61.78

Gene sequence:

>798_bases
ATGAAGAAGTTCGCAGCACTACTCCTCATTTCGACCGTCGCCCTACTCGGTGCGTGCGGCAAGAAGGAATCGGCACCTGC
CGCCGGCACGGAGCCGGCTCCGGTTGCCGCTACAAAGATCATCGTCGGCCTCGACGACAATTTCCCGCCGATGGGCTTCC
GCGACAGCAATAACGAGCTCGTCGGTTTCGACATCGACATGGCCAAGGAGGCGAGCCGCCGCCTCGGCTTGACGGTCGAG
TTCAAGCCGATCGACTGGAGCGCCAAGGAAGCCGAGCTCAATGGCAAGCGCGTGGATGTGCTGTGGAACGGCCTGACCAT
TACGGAAGAGCGCAAGCAGAACATCAGCTTCACCGCGCCCTATATGACGAACCACCAGATCGTCATTGTCGGCACCAATT
CGCCGGTGAAGACCAAGGCTGACCTGTCGGGCCGCATTGTCGGCGCACAGGATGGCAGCAGCGCCGTGGACGCGATCAAG
AAGGAAGCGCAGGTCGCGGGCAGCCTGAAGGAACTGAAGACCTTCGGCGACAACGTGACGGCGCTGATGGACCTGTCGGC
CGGCCGCCTCGATGCGATCGTGGTCGACGAAGTCGTGGGCCGCTACCTGATCAGCAAGCGCGCAGGCGAATACCGTGTAC
TCGACGAGAACTTCGGTACGGAAGACTACGGCGTAGGCGTGCGCAAGGACGATGCCGAGCTGCTTGGCAAACTCGACAAG
ACGCTCCATTCGATGAAGGAAGATGGCACGGCGGCCCGCATCGCCACCCGCTGGTTCGGCGCCGACATCACCAAGTAA

Upstream 100 bases:

>100_bases
GGGGCGTCGCTCTATAATCGCGCTGCCAGCAATAACCTGAAATCCGCCGGTCTCCGTACCCGGGACACCGGCGCGCCGTC
TATTTGTATTGGATACCTCC

Downstream 100 bases:

>100_bases
TCCGGATCGTTCGCGACGTCCCGTGCCCCATTGGCGCGGCGTCGCGCCTCATCGCGGCCCGCGCGCCGGCAAAGTAGCAG
CAGCCAGGCGCCCGGGATCC

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: CBP; Protein fliY; Sulfate starvation-induced protein 7; SSI7 [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MKKFAALLLISTVALLGACGKKESAPAAGTEPAPVAATKIIVGLDDNFPPMGFRDSNNELVGFDIDMAKEASRRLGLTVE
FKPIDWSAKEAELNGKRVDVLWNGLTITEERKQNISFTAPYMTNHQIVIVGTNSPVKTKADLSGRIVGAQDGSSAVDAIK
KEAQVAGSLKELKTFGDNVTALMDLSAGRLDAIVVDEVVGRYLISKRAGEYRVLDENFGTEDYGVGVRKDDAELLGKLDK
TLHSMKEDGTAARIATRWFGADITK

Sequences:

>Translated_265_residues
MKKFAALLLISTVALLGACGKKESAPAAGTEPAPVAATKIIVGLDDNFPPMGFRDSNNELVGFDIDMAKEASRRLGLTVE
FKPIDWSAKEAELNGKRVDVLWNGLTITEERKQNISFTAPYMTNHQIVIVGTNSPVKTKADLSGRIVGAQDGSSAVDAIK
KEAQVAGSLKELKTFGDNVTALMDLSAGRLDAIVVDEVVGRYLISKRAGEYRVLDENFGTEDYGVGVRKDDAELLGKLDK
TLHSMKEDGTAARIATRWFGADITK
>Mature_265_residues
MKKFAALLLISTVALLGACGKKESAPAAGTEPAPVAATKIIVGLDDNFPPMGFRDSNNELVGFDIDMAKEASRRLGLTVE
FKPIDWSAKEAELNGKRVDVLWNGLTITEERKQNISFTAPYMTNHQIVIVGTNSPVKTKADLSGRIVGAQDGSSAVDAIK
KEAQVAGSLKELKTFGDNVTALMDLSAGRLDAIVVDEVVGRYLISKRAGEYRVLDENFGTEDYGVGVRKDDAELLGKLDK
TLHSMKEDGTAARIATRWFGADITK

Specific function: Part of a binding-protein-dependent transport system for cystine [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=262, Percent_Identity=31.6793893129771, Blast_Score=147, Evalue=7e-37,
Organism=Escherichia coli, GI1787031, Length=229, Percent_Identity=31.8777292576419, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1787085, Length=230, Percent_Identity=32.1739130434783, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788649, Length=228, Percent_Identity=29.8245614035088, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1788648, Length=233, Percent_Identity=32.1888412017167, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1787088, Length=243, Percent_Identity=28.8065843621399, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28546; Mature: 28546

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFAALLLISTVALLGACGKKESAPAAGTEPAPVAATKIIVGLDDNFPPMGFRDSNNEL
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCEECCCCCE
VGFDIDMAKEASRRLGLTVEFKPIDWSAKEAELNGKRVDVLWNGLTITEERKQNISFTAP
EEEEHHHHHHHHHHCCCEEEEECCCCCCCHHCCCCCEEEEEECCEEEEHHHHCCCEEECC
YMTNHQIVIVGTNSPVKTKADLSGRIVGAQDGSSAVDAIKKEAQVAGSLKELKTFGDNVT
EECCCEEEEEECCCCCCEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
ALMDLSAGRLDAIVVDEVVGRYLISKRAGEYRVLDENFGTEDYGVGVRKDDAELLGKLDK
EEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHCCCCCCCHHHHHHHHHH
TLHSMKEDGTAARIATRWFGADITK
HHHHHHCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKFAALLLISTVALLGACGKKESAPAAGTEPAPVAATKIIVGLDDNFPPMGFRDSNNEL
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCEECCCCCE
VGFDIDMAKEASRRLGLTVEFKPIDWSAKEAELNGKRVDVLWNGLTITEERKQNISFTAP
EEEEHHHHHHHHHHCCCEEEEECCCCCCCHHCCCCCEEEEEECCEEEEHHHHCCCEEECC
YMTNHQIVIVGTNSPVKTKADLSGRIVGAQDGSSAVDAIKKEAQVAGSLKELKTFGDNVT
EECCCEEEEEECCCCCCEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
ALMDLSAGRLDAIVVDEVVGRYLISKRAGEYRVLDENFGTEDYGVGVRKDDAELLGKLDK
EEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHCCCCCCCHHHHHHHHHH
TLHSMKEDGTAARIATRWFGADITK
HHHHHHCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]