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The map label for this gene is dppA [H]

Identifier: 73542690

GI number: 73542690

Start: 3302354

End: 3303940

Strand: Reverse

Name: dppA [H]

Synonym: Reut_A3006

Alternate gene names: 73542690

Gene position: 3303940-3302354 (Counterclockwise)

Preceding gene: 73542693

Following gene: 73542689

Centisome position: 86.8

GC content: 59.99

Gene sequence:

>1587_bases
ATGTCCCTTCGCACAATCAAGAAGGCATTTGGTGCTGTCGCGGTAGCCGGGGCCATGAGCCTTGTGCTGGCCGGCGCGGC
ACAGGCCGCTGACCTCAAGCTTGCCATGAGTTCGCCGCCGACCTCGATGGATCCGCACTTCTACAATCTGTTTTCGAACA
TCAACGTGTCTGAACACGTGTTCGAGACGATGATCAAGATGGACCCCGACAGCCGCATCATTCCCGGCCTCGCGGAGTCG
TGGAAGATGATCAACAACCTGACCTGGGAATTCAAGATCCGCAAGGGCGTGAAGTTCCATGACGGCTCCGAGCTGACCAC
CGAGGACATCGTCTGGTCGCTGGACCGGCCGGCCACCATTCAGAACAGTCCCGGCAAGTTCGACGTCTACACCAAGGCGA
TCATCAACAAGAAGGTGATCGACAAGTACACCATCCAGCTCACTACCAACCAGCCGTATCCGCTCATGCTGAACGACCTC
ACGTCGATCTTCGTCGTGCAGAAGAAGGCCACGCAAGGGTTGAGTTCGGATGACTTCGCCCAGGGCAAGGGCATGATCGG
CACCGGCCCGTTCAAGTTCGTGAGCTACGCGCGCGACGACCGGGTCGAACTGGTGCGCAATCCCGATTACTGGGGCCCGA
AGCCCGCGTGGGACAAGGTGACGCTGCGCTTCATTCCCAATCCGGCCACGCGCCTCGCGGCGCTGCTGTCCGGTGATGTG
CAGGCGATCGAGAACGTGCCGACGCCGGATCTGCCGAAGGTGCGCAACGATCCCAAGTTGTCGTTCTTCTCCAAGATTTC
GCACCGCGTGATCTACCTGTACTTCGATGCCAAGCGTGACAAGTCGCCGTACGTCACGACCAAGGAAGGTCAGCCGATGG
ACAAGAACCCGCTGAAGGATGCGCGCGTGCGCAATGCCATCAGCATGGCCATCAACCGGCAGGGCATCAAGGACCGACTG
ATGGAAGGCCTGTCCGAGCCGACCAACAATCTCGTGCCGCCCACGCTGTTCGGTTATAACCCGAACCTCAAGACGGTCAA
GTACGATCCCGAGGGCGCGAAGAAGCTGCTCGCGGAAGCGGGGTATCCGAATGGCTTTGGCGTGACGCTGCACACGCCTA
ACAACCGCTACGTCAACGACGAGAAGATTGCGCAGACCATCGCGCAGAACCTCACGCGCGTCGGCATCGCGACGAAGGTG
GAAGGCATGCCGATGGCGACGTATTCGTCCAAGGGCATCAAGCACGAATGGTCGTTCGGCTTGCTCGGCTGGGGCGCACA
GACCGGAGAAGTCAGCTCGCCGCTGCGCGCGCTGCTGGCGTGCGAGGACAGCAAGAAGGGCTTCGGCACCACTAACTGGG
GCGAATATTGCAATCCGAAGATGGATGTGGTGCTGGAGAAGGCGCTGGCCACGGTCGATGACAACGAGCGTTCCAAGCTG
CTGCAGGAAGCCACTGCCATTGCTATCAACGACGGCGGTATCATCCCGATCCACCAGCAGGTGACCACCTGGGCCACGCA
GAAGGGTATCGTCTATATTCCGCGCACCGACGAACGCACCTATGCGCACAACTTCAAGCCGCAGTAA

Upstream 100 bases:

>100_bases
GCCTGACCGCACCAAAATGCAGCGCTCTTTCGTGTGATGCTGCGGCGCGTCCATTGCCGATCCTAACGTCTGCTTCATCT
GTTCCTGGGAGAATTGCGCG

Downstream 100 bases:

>100_bases
GCGGCGCCATCCGGTCGCCTGCGGGTAGTGCTCGCGGCAACGTACACCATGCGGGCCGTGCCTGGGACGGGCGCGGGGCT
GGCCCGGCGCGACCCGCAGC

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MSLRTIKKAFGAVAVAGAMSLVLAGAAQAADLKLAMSSPPTSMDPHFYNLFSNINVSEHVFETMIKMDPDSRIIPGLAES
WKMINNLTWEFKIRKGVKFHDGSELTTEDIVWSLDRPATIQNSPGKFDVYTKAIINKKVIDKYTIQLTTNQPYPLMLNDL
TSIFVVQKKATQGLSSDDFAQGKGMIGTGPFKFVSYARDDRVELVRNPDYWGPKPAWDKVTLRFIPNPATRLAALLSGDV
QAIENVPTPDLPKVRNDPKLSFFSKISHRVIYLYFDAKRDKSPYVTTKEGQPMDKNPLKDARVRNAISMAINRQGIKDRL
MEGLSEPTNNLVPPTLFGYNPNLKTVKYDPEGAKKLLAEAGYPNGFGVTLHTPNNRYVNDEKIAQTIAQNLTRVGIATKV
EGMPMATYSSKGIKHEWSFGLLGWGAQTGEVSSPLRALLACEDSKKGFGTTNWGEYCNPKMDVVLEKALATVDDNERSKL
LQEATAIAINDGGIIPIHQQVTTWATQKGIVYIPRTDERTYAHNFKPQ

Sequences:

>Translated_528_residues
MSLRTIKKAFGAVAVAGAMSLVLAGAAQAADLKLAMSSPPTSMDPHFYNLFSNINVSEHVFETMIKMDPDSRIIPGLAES
WKMINNLTWEFKIRKGVKFHDGSELTTEDIVWSLDRPATIQNSPGKFDVYTKAIINKKVIDKYTIQLTTNQPYPLMLNDL
TSIFVVQKKATQGLSSDDFAQGKGMIGTGPFKFVSYARDDRVELVRNPDYWGPKPAWDKVTLRFIPNPATRLAALLSGDV
QAIENVPTPDLPKVRNDPKLSFFSKISHRVIYLYFDAKRDKSPYVTTKEGQPMDKNPLKDARVRNAISMAINRQGIKDRL
MEGLSEPTNNLVPPTLFGYNPNLKTVKYDPEGAKKLLAEAGYPNGFGVTLHTPNNRYVNDEKIAQTIAQNLTRVGIATKV
EGMPMATYSSKGIKHEWSFGLLGWGAQTGEVSSPLRALLACEDSKKGFGTTNWGEYCNPKMDVVLEKALATVDDNERSKL
LQEATAIAINDGGIIPIHQQVTTWATQKGIVYIPRTDERTYAHNFKPQ
>Mature_527_residues
SLRTIKKAFGAVAVAGAMSLVLAGAAQAADLKLAMSSPPTSMDPHFYNLFSNINVSEHVFETMIKMDPDSRIIPGLAESW
KMINNLTWEFKIRKGVKFHDGSELTTEDIVWSLDRPATIQNSPGKFDVYTKAIINKKVIDKYTIQLTTNQPYPLMLNDLT
SIFVVQKKATQGLSSDDFAQGKGMIGTGPFKFVSYARDDRVELVRNPDYWGPKPAWDKVTLRFIPNPATRLAALLSGDVQ
AIENVPTPDLPKVRNDPKLSFFSKISHRVIYLYFDAKRDKSPYVTTKEGQPMDKNPLKDARVRNAISMAINRQGIKDRLM
EGLSEPTNNLVPPTLFGYNPNLKTVKYDPEGAKKLLAEAGYPNGFGVTLHTPNNRYVNDEKIAQTIAQNLTRVGIATKVE
GMPMATYSSKGIKHEWSFGLLGWGAQTGEVSSPLRALLACEDSKKGFGTTNWGEYCNPKMDVVLEKALATVDDNERSKLL
QEATAIAINDGGIIPIHQQVTTWATQKGIVYIPRTDERTYAHNFKPQ

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=500, Percent_Identity=26, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1787052, Length=499, Percent_Identity=28.2565130260521, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI1787762, Length=524, Percent_Identity=25.5725190839695, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1787551, Length=487, Percent_Identity=23.8193018480493, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1787495, Length=536, Percent_Identity=24.2537313432836, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1789887, Length=521, Percent_Identity=24.5681381957774, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI87081878, Length=501, Percent_Identity=24.1516966067864, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1789397, Length=492, Percent_Identity=23.5772357723577, Blast_Score=109, Evalue=4e-25,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58678; Mature: 58547

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRTIKKAFGAVAVAGAMSLVLAGAAQAADLKLAMSSPPTSMDPHFYNLFSNINVSEHV
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCHHHHH
FETMIKMDPDSRIIPGLAESWKMINNLTWEFKIRKGVKFHDGSELTTEDIVWSLDRPATI
HHHHHHCCCCCCCCCCHHHHHHHHHCCEEEEEEECCCEECCCCCCCHHHHEEECCCCCEE
QNSPGKFDVYTKAIINKKVIDKYTIQLTTNQPYPLMLNDLTSIFVVQKKATQGLSSDDFA
CCCCCCEEHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHCEEEEEEEHHHCCCCCCCHH
QGKGMIGTGPFKFVSYARDDRVELVRNPDYWGPKPAWDKVTLRFIPNPATRLAALLSGDV
CCCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCH
QAIENVPTPDLPKVRNDPKLSFFSKISHRVIYLYFDAKRDKSPYVTTKEGQPMDKNPLKD
HHHHCCCCCCCCCCCCCCCHHHHHHCCCEEEEEEEECCCCCCCCEECCCCCCCCCCCCHH
ARVRNAISMAINRQGIKDRLMEGLSEPTNNLVPPTLFGYNPNLKTVKYDPEGAKKLLAEA
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEECCCCCCCEEEECCHHHHHHHHHC
GYPNGFGVTLHTPNNRYVNDEKIAQTIAQNLTRVGIATKVEGMPMATYSSKGIKHEWSFG
CCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCEEEECCCCCCCCCCCCE
LLGWGAQTGEVSSPLRALLACEDSKKGFGTTNWGEYCNPKMDVVLEKALATVDDNERSKL
EEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCHHHHH
LQEATAIAINDGGIIPIHQQVTTWATQKGIVYIPRTDERTYAHNFKPQ
HHHHHEEEECCCCEEEEHHHHHHHHCCCCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
SLRTIKKAFGAVAVAGAMSLVLAGAAQAADLKLAMSSPPTSMDPHFYNLFSNINVSEHV
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCHHHHH
FETMIKMDPDSRIIPGLAESWKMINNLTWEFKIRKGVKFHDGSELTTEDIVWSLDRPATI
HHHHHHCCCCCCCCCCHHHHHHHHHCCEEEEEEECCCEECCCCCCCHHHHEEECCCCCEE
QNSPGKFDVYTKAIINKKVIDKYTIQLTTNQPYPLMLNDLTSIFVVQKKATQGLSSDDFA
CCCCCCEEHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHCEEEEEEEHHHCCCCCCCHH
QGKGMIGTGPFKFVSYARDDRVELVRNPDYWGPKPAWDKVTLRFIPNPATRLAALLSGDV
CCCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCH
QAIENVPTPDLPKVRNDPKLSFFSKISHRVIYLYFDAKRDKSPYVTTKEGQPMDKNPLKD
HHHHCCCCCCCCCCCCCCCHHHHHHCCCEEEEEEEECCCCCCCCEECCCCCCCCCCCCHH
ARVRNAISMAINRQGIKDRLMEGLSEPTNNLVPPTLFGYNPNLKTVKYDPEGAKKLLAEA
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEECCCCCCCEEEECCHHHHHHHHHC
GYPNGFGVTLHTPNNRYVNDEKIAQTIAQNLTRVGIATKVEGMPMATYSSKGIKHEWSFG
CCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCEEEECCCCCCCCCCCCE
LLGWGAQTGEVSSPLRALLACEDSKKGFGTTNWGEYCNPKMDVVLEKALATVDDNERSKL
EEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCHHHHH
LQEATAIAINDGGIIPIHQQVTTWATQKGIVYIPRTDERTYAHNFKPQ
HHHHHEEEECCCCEEEEHHHHHHHHCCCCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]