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The map label for this gene is yoaE [H]

Identifier: 73542684

GI number: 73542684

Start: 3294593

End: 3296704

Strand: Reverse

Name: yoaE [H]

Synonym: Reut_A3000

Alternate gene names: 73542684

Gene position: 3296704-3294593 (Counterclockwise)

Preceding gene: 73542686

Following gene: 73542683

Centisome position: 86.61

GC content: 68.37

Gene sequence:

>2112_bases
ATGGCTTCCCGCATTGTCCGCGCCGCCTGCCCGCATGATTGCCCCGACACCTGTGCCTTGCTCGTCACCGTCGAGGACGG
CCGCGCCATCAAGGTCAACGGAGACCCGGATCACCCGGGCACGCAGGGCGTGCTGTGCACCAAGGTGTCGCGCTATACCG
AGCGCACCTATCACCCTGACCGCCTGTTGACGCCGATGCGCCGCGTCGGGCGCAAGGGCGAGGGCAAGTTCGAGCCGATT
TCCTGGGACGAAGCACTTGACGAAGTCGCCACGCGGCTCAAGGCCATTGCCGTGCGCGATCCGCAGGCGATCGTGCCGTA
CAGCTACGCCGGCACCATGGGGCTGGTGCAGGGCGAGAGCATGGCCGCGCGCTTCTTCCACAAGCTCGGCGCCTCGCTGC
TCGACCGCACGATCTGCGCGAGTGCCGGCGCCACGGCGCTGCGCTACACCTATGGCGCGAGCGTCGGCATGGACATGGAA
AACGTCGTCGATGCGAAGCTCGTGATCATCTGGGGCGGCAACCCGATCGCCTCGAACCTGCACTTCTGGACGCGCGCGCA
GGAAGCCAAGCGCCGCGGCGCGACGCTGGTGGCGATCGACCCGTACCGCTCGCTGACCGCCGAGAAGTGCCACCGCCATA
TTGCGCCGATGCCGGGCACGGACGGCGCGCTGGCGCTGGCCATCATGCACGTGCTGATCCGCGACGACCTGCTCGACCAC
GACTACATCGCGCAGCACACGCTCGGCTTCGACGCGCTGCGCGAGCGCGCGCAAGCGTATTCGCCAGCCCGCGCCGCGGA
AATCTGCGGGATTCCGGTGGAGGACATTGAATGGCTGGCCGGCCTCTACGGCATGCTGGCGGTACGTGAGCGCCAGCCGG
CGGCGATCCGCCTGAACTACGGCATGCAGCGCGTGCACGGTGGCGGGCAGGCGGTGCGCGCGGTCGCGTGCCTGCCTTCG
CTGGTTGGTGCGTGGCGGCATCCGGCGGGCGGGCTTCAGCTCTCGACGTCAGGATTCTTCCCGGTCAACGACCGGGCCTT
GCAGCGGCCTGACCTGCTGCCAGCCGGCCGGCTGCCGCGCACGGTCAACATGAGCACGATCGGCGACGCGCTGCTGGACG
TGGGGGCGCCGGGCAAGCCGCGCCTCGAAGCCGTGGTGGTCTACAACAGCAACCCGGTGGCCGTGGCGCCGCAATCGGGC
AAGGTGGCCGAGGGCTTTGCGCGCGAGGACCTGTTCACGGTCGTGCTGGAGCAGTTCCGCACCGATACGGCGGACTACGC
CGACATCCTGCTGCCGGCCACCACGCAGCTCGAACACGTGGATGTGCACAAGGCCTACGGGCACACATACTTCCTCGCCA
ACAACGCCGCCATCGAGCCGATGGGCCAGGCCTTGCCGAACACCGAGATCTTCCGCCGCCTGGCGCAGCGCATGGGCTTC
AGTGATCCCTGTTTTGCCGACAGCGATGAGCAGATCGCCGCGCAGGCCATTGTCCCGGGCGACCAGCGCGCACAGGGCAT
CACGTGGGAATCGCTGAAGGAGAAGGGCTGGCAGAAGCTGTCGCTGCCGAGCGCGCCGTTCGCGCACGGCGGTTTTCCGA
CTGCGTCCGGCAAGTGCGAGTTCTATTCCGAAGCCATGCGTAGCGCGGGCCTCGATCCGGTGCCGGACTATGTAGCGCCG
TACGAGGCACCTTCGTCCGCACCGGAACTGGCTGGCAAGTATCCACTGGCGATGATCTCGCCGCCCGCGCGCAACTTCCT
GAACAGCTCCTTTGTCAACGTCGACAGCCTGCGCGCGACCGAGGGCGAGCCGCACCTGGACATCCACCCCGCCGATGCGG
ATGCGCGCGGCATTGCCAACGGCGCCCTGGTGCGCGCGTTCAACGACCGCGGCAGCATGCTCGCGCGCGCACGGGTGACC
GACCGTGCGCGGCGCGGGCTGGTGGTGGGCCTGTCGATCTGGTGGAAAAAACTGGCGCCGGACGGCAAGAACGCCAACGA
GCTGACCAGCCAGCGCCTGACGGATCTGGGCCGCGCGCCCGTGTTCTACGATTGCCTGGTCGAGGTGGAGGCATGGCAGG
AAGTCGGCGAGGCAGCGGCGGCTGCCTCATGA

Upstream 100 bases:

>100_bases
GTCCGCCGTGGTTTTCCCGGTAGTGACAGGCATCCGGCCCATCCCCCTGGCCATTCGCCCCGATCGCACCCAGCCGCCTT
CCTGCCCGCCCGGAGATTCC

Downstream 100 bases:

>100_bases
TGCAGTGATGGACTGGAAAAAGGTTCGCCGGCGGACGGCGGGCATCCTTGCAGCGGCGGGGCTGACGCTGGCGGTGGCCG
GCTGTGACACGCTCGGCTAT

Product: molybdopterin oxidoreductase

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 703; Mature: 702

Protein sequence:

>703_residues
MASRIVRAACPHDCPDTCALLVTVEDGRAIKVNGDPDHPGTQGVLCTKVSRYTERTYHPDRLLTPMRRVGRKGEGKFEPI
SWDEALDEVATRLKAIAVRDPQAIVPYSYAGTMGLVQGESMAARFFHKLGASLLDRTICASAGATALRYTYGASVGMDME
NVVDAKLVIIWGGNPIASNLHFWTRAQEAKRRGATLVAIDPYRSLTAEKCHRHIAPMPGTDGALALAIMHVLIRDDLLDH
DYIAQHTLGFDALRERAQAYSPARAAEICGIPVEDIEWLAGLYGMLAVRERQPAAIRLNYGMQRVHGGGQAVRAVACLPS
LVGAWRHPAGGLQLSTSGFFPVNDRALQRPDLLPAGRLPRTVNMSTIGDALLDVGAPGKPRLEAVVVYNSNPVAVAPQSG
KVAEGFAREDLFTVVLEQFRTDTADYADILLPATTQLEHVDVHKAYGHTYFLANNAAIEPMGQALPNTEIFRRLAQRMGF
SDPCFADSDEQIAAQAIVPGDQRAQGITWESLKEKGWQKLSLPSAPFAHGGFPTASGKCEFYSEAMRSAGLDPVPDYVAP
YEAPSSAPELAGKYPLAMISPPARNFLNSSFVNVDSLRATEGEPHLDIHPADADARGIANGALVRAFNDRGSMLARARVT
DRARRGLVVGLSIWWKKLAPDGKNANELTSQRLTDLGRAPVFYDCLVEVEAWQEVGEAAAAAS

Sequences:

>Translated_703_residues
MASRIVRAACPHDCPDTCALLVTVEDGRAIKVNGDPDHPGTQGVLCTKVSRYTERTYHPDRLLTPMRRVGRKGEGKFEPI
SWDEALDEVATRLKAIAVRDPQAIVPYSYAGTMGLVQGESMAARFFHKLGASLLDRTICASAGATALRYTYGASVGMDME
NVVDAKLVIIWGGNPIASNLHFWTRAQEAKRRGATLVAIDPYRSLTAEKCHRHIAPMPGTDGALALAIMHVLIRDDLLDH
DYIAQHTLGFDALRERAQAYSPARAAEICGIPVEDIEWLAGLYGMLAVRERQPAAIRLNYGMQRVHGGGQAVRAVACLPS
LVGAWRHPAGGLQLSTSGFFPVNDRALQRPDLLPAGRLPRTVNMSTIGDALLDVGAPGKPRLEAVVVYNSNPVAVAPQSG
KVAEGFAREDLFTVVLEQFRTDTADYADILLPATTQLEHVDVHKAYGHTYFLANNAAIEPMGQALPNTEIFRRLAQRMGF
SDPCFADSDEQIAAQAIVPGDQRAQGITWESLKEKGWQKLSLPSAPFAHGGFPTASGKCEFYSEAMRSAGLDPVPDYVAP
YEAPSSAPELAGKYPLAMISPPARNFLNSSFVNVDSLRATEGEPHLDIHPADADARGIANGALVRAFNDRGSMLARARVT
DRARRGLVVGLSIWWKKLAPDGKNANELTSQRLTDLGRAPVFYDCLVEVEAWQEVGEAAAAAS
>Mature_702_residues
ASRIVRAACPHDCPDTCALLVTVEDGRAIKVNGDPDHPGTQGVLCTKVSRYTERTYHPDRLLTPMRRVGRKGEGKFEPIS
WDEALDEVATRLKAIAVRDPQAIVPYSYAGTMGLVQGESMAARFFHKLGASLLDRTICASAGATALRYTYGASVGMDMEN
VVDAKLVIIWGGNPIASNLHFWTRAQEAKRRGATLVAIDPYRSLTAEKCHRHIAPMPGTDGALALAIMHVLIRDDLLDHD
YIAQHTLGFDALRERAQAYSPARAAEICGIPVEDIEWLAGLYGMLAVRERQPAAIRLNYGMQRVHGGGQAVRAVACLPSL
VGAWRHPAGGLQLSTSGFFPVNDRALQRPDLLPAGRLPRTVNMSTIGDALLDVGAPGKPRLEAVVVYNSNPVAVAPQSGK
VAEGFAREDLFTVVLEQFRTDTADYADILLPATTQLEHVDVHKAYGHTYFLANNAAIEPMGQALPNTEIFRRLAQRMGFS
DPCFADSDEQIAAQAIVPGDQRAQGITWESLKEKGWQKLSLPSAPFAHGGFPTASGKCEFYSEAMRSAGLDPVPDYVAPY
EAPSSAPELAGKYPLAMISPPARNFLNSSFVNVDSLRATEGEPHLDIHPADADARGIANGALVRAFNDRGSMLARARVTD
RARRGLVVGLSIWWKKLAPDGKNANELTSQRLTDLGRAPVFYDCLVEVEAWQEVGEAAAAAS

Specific function: Terminal Reductase During Anaerobic Growth On Various Sulfoxide And N-Oxide Compounds. Allows E.Coli To Grow Anaerobically On Me(2)So As Respiratory Oxidant. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=776, Percent_Identity=27.4484536082474, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1787870, Length=776, Percent_Identity=28.3505154639175, Blast_Score=209, Evalue=6e-55,
Organism=Escherichia coli, GI171474008, Length=774, Percent_Identity=27.5193798449612, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI3868721, Length=735, Percent_Identity=26.6666666666667, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1787231, Length=812, Percent_Identity=24.5073891625616, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI87081994, Length=712, Percent_Identity=24.8595505617978, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI145693196, Length=660, Percent_Identity=25, Blast_Score=128, Evalue=2e-30,
Organism=Escherichia coli, GI1788534, Length=312, Percent_Identity=21.7948717948718, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI3868720, Length=277, Percent_Identity=25.9927797833935, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI3868719, Length=408, Percent_Identity=23.0392156862745, Blast_Score=77, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 76127; Mature: 75996

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00490 MOLYBDOPTERIN_PROK_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASRIVRAACPHDCPDTCALLVTVEDGRAIKVNGDPDHPGTQGVLCTKVSRYTERTYHPD
CCCCHHHHCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCH
RLLTPMRRVGRKGEGKFEPISWDEALDEVATRLKAIAVRDPQAIVPYSYAGTMGLVQGES
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCEECCCCCCCCEEEECCHH
MAARFFHKLGASLLDRTICASAGATALRYTYGASVGMDMENVVDAKLVIIWGGNPIASNL
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHCCCEEEEEEECCCCCCCCC
HFWTRAQEAKRRGATLVAIDPYRSLTAEKCHRHIAPMPGTDGALALAIMHVLIRDDLLDH
HHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCC
DYIAQHTLGFDALRERAQAYSPARAAEICGIPVEDIEWLAGLYGMLAVRERQPAAIRLNY
HHHHHHHCCHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECC
GMQRVHGGGQAVRAVACLPSLVGAWRHPAGGLQLSTSGFFPVNDRALQRPDLLPAGRLPR
CHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHCCCCCCCCCCCCCC
TVNMSTIGDALLDVGAPGKPRLEAVVVYNSNPVAVAPQSGKVAEGFAREDLFTVVLEQFR
EECHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHC
TDTADYADILLPATTQLEHVDVHKAYGHTYFLANNAAIEPMGQALPNTEIFRRLAQRMGF
CCCCCHHHEEECCCCCCHHEEHHHHCCCEEEEECCCCCCCHHHHCCCHHHHHHHHHHCCC
SDPCFADSDEQIAAQAIVPGDQRAQGITWESLKEKGWQKLSLPSAPFAHGGFPTASGKCE
CCCCCCCCCHHHHHHEECCCCCCCCCCCHHHHHHCCCCEECCCCCCCCCCCCCCCCCCHH
FYSEAMRSAGLDPVPDYVAPYEAPSSAPELAGKYPLAMISPPARNFLNSSFVNVDSLRAT
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCEEEECCHHHHHHCCCCCCCCCCCCC
EGEPHLDIHPADADARGIANGALVRAFNDRGSMLARARVTDRARRGLVVGLSIWWKKLAP
CCCCCEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCEEEEHHHHHHHHCC
DGKNANELTSQRLTDLGRAPVFYDCLVEVEAWQEVGEAAAAAS
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ASRIVRAACPHDCPDTCALLVTVEDGRAIKVNGDPDHPGTQGVLCTKVSRYTERTYHPD
CCCHHHHCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCH
RLLTPMRRVGRKGEGKFEPISWDEALDEVATRLKAIAVRDPQAIVPYSYAGTMGLVQGES
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCEECCCCCCCCEEEECCHH
MAARFFHKLGASLLDRTICASAGATALRYTYGASVGMDMENVVDAKLVIIWGGNPIASNL
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHCCCEEEEEEECCCCCCCCC
HFWTRAQEAKRRGATLVAIDPYRSLTAEKCHRHIAPMPGTDGALALAIMHVLIRDDLLDH
HHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCC
DYIAQHTLGFDALRERAQAYSPARAAEICGIPVEDIEWLAGLYGMLAVRERQPAAIRLNY
HHHHHHHCCHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECC
GMQRVHGGGQAVRAVACLPSLVGAWRHPAGGLQLSTSGFFPVNDRALQRPDLLPAGRLPR
CHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHCCCCCCCCCCCCCC
TVNMSTIGDALLDVGAPGKPRLEAVVVYNSNPVAVAPQSGKVAEGFAREDLFTVVLEQFR
EECHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHC
TDTADYADILLPATTQLEHVDVHKAYGHTYFLANNAAIEPMGQALPNTEIFRRLAQRMGF
CCCCCHHHEEECCCCCCHHEEHHHHCCCEEEEECCCCCCCHHHHCCCHHHHHHHHHHCCC
SDPCFADSDEQIAAQAIVPGDQRAQGITWESLKEKGWQKLSLPSAPFAHGGFPTASGKCE
CCCCCCCCCHHHHHHEECCCCCCCCCCCHHHHHHCCCCEECCCCCCCCCCCCCCCCCCHH
FYSEAMRSAGLDPVPDYVAPYEAPSSAPELAGKYPLAMISPPARNFLNSSFVNVDSLRAT
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCEEEECCHHHHHHCCCCCCCCCCCCC
EGEPHLDIHPADADARGIANGALVRAFNDRGSMLARARVTDRARRGLVVGLSIWWKKLAP
CCCCCEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCEEEEHHHHHHHHCC
DGKNANELTSQRLTDLGRAPVFYDCLVEVEAWQEVGEAAAAAS
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]