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The map label for this gene is ddl [H]

Identifier: 73542661

GI number: 73542661

Start: 3265306

End: 3266289

Strand: Reverse

Name: ddl [H]

Synonym: Reut_A2977

Alternate gene names: 73542661

Gene position: 3266289-3265306 (Counterclockwise)

Preceding gene: 73542662

Following gene: 73542660

Centisome position: 85.81

GC content: 65.14

Gene sequence:

>984_bases
ATGAGCTTCGTCGCCAATCCCAAGATCGACCCGAAATCGCTGGGCAAGGTCGGCGTGCTGTATGGCGGCCGCTCGGCCGA
GCGCGAGATTTCGCTGATGTCCGGCAGCGGCGTGCTGGCCGCGCTGCAGTCGCGCGGCGTGGATGCGCACGGCTTTGACC
CGGCCACGCAGAGCGTGGCCGAACTGGCCGCGGCCGGCTTCGACCGCGTCTTCATCGCGCTGCACGGCCGCTACGGCGAG
GACGGCACCATGCAGGGCCTGCTCGAGCAGCTCGGCGTGCCCTACACCGGCAGCGGCGTGCTGGCGTCGGCGCTGGCCAT
GGACAAGCAGGCGACCAAGCGCCTGTGGATGACGCATGACCTGTCCACGCCGCGTTTCGCGATGCTCTATGCCGATACGG
ACTTCGATGCAGTCGTCGCCGATCTCGGCCTGCCGCTGATCGTCAAGCCGGCGCGCGAAGGCTCGTCGATCGGTCTGAGC
AAGGTCACCGATGCGTCGCAGATGCGCGAGGCATTCGAGAAGGCTGCCGCGCTCGACAACGACGTCATTGCAGAGACCTT
CATTGACGGCGCCGAGCTGACCTGCCCGTTGGTAGGGGAGGGCGCTACGGCTGAAGCGCTGCCGGTGATTCGCATCGTCG
CGCCGGATTCTAACTATGACTATCAAAATAAGTACTTTACTGACGATACCCAATATCTGTGTCCGTCAGGCCTGAGTGCT
GAAGTCGAGCGCGAGGTGCAGCAACTGGCGGTGCAGGCCTACCGCGTGCTGGGCTGCCGTGGCTGGGCGCGTGCGGACGT
GATGCTGCGCGCCGACGGCAAGCCTTTCCTGCTTGAGATGAATACCTCGCCTGGCATGACCGGCCATTCGCTCGTGCCGA
TGGCCGCGCGCGCGGCTGGCATCAGCTATGAGGACTTCGTCATGCAGGTGGTGGCCGCCGCGACGCTGGACCTGCATCCG
AACGAACACTGGAAACCCGAATAA

Upstream 100 bases:

>100_bases
GGCCTGGCGACGTGGTCGTAACGATGGGAGCCGGCTCGATCGGCGCGGTACCGGGACAGGTGGTCTCGCACCAGCAGCCG
CTGCAGGCGGGAGGTGCGGC

Downstream 100 bases:

>100_bases
CCCTGGCAAGACCATACCGGACGACCCTGGAACATGTGGCATAACGCACGCCTGCTCAACCTGATCGCTTCCGCGCTGTA
CGCGCTGGTGGCGCTGATGG

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MSFVANPKIDPKSLGKVGVLYGGRSAEREISLMSGSGVLAALQSRGVDAHGFDPATQSVAELAAAGFDRVFIALHGRYGE
DGTMQGLLEQLGVPYTGSGVLASALAMDKQATKRLWMTHDLSTPRFAMLYADTDFDAVVADLGLPLIVKPAREGSSIGLS
KVTDASQMREAFEKAAALDNDVIAETFIDGAELTCPLVGEGATAEALPVIRIVAPDSNYDYQNKYFTDDTQYLCPSGLSA
EVEREVQQLAVQAYRVLGCRGWARADVMLRADGKPFLLEMNTSPGMTGHSLVPMAARAAGISYEDFVMQVVAAATLDLHP
NEHWKPE

Sequences:

>Translated_327_residues
MSFVANPKIDPKSLGKVGVLYGGRSAEREISLMSGSGVLAALQSRGVDAHGFDPATQSVAELAAAGFDRVFIALHGRYGE
DGTMQGLLEQLGVPYTGSGVLASALAMDKQATKRLWMTHDLSTPRFAMLYADTDFDAVVADLGLPLIVKPAREGSSIGLS
KVTDASQMREAFEKAAALDNDVIAETFIDGAELTCPLVGEGATAEALPVIRIVAPDSNYDYQNKYFTDDTQYLCPSGLSA
EVEREVQQLAVQAYRVLGCRGWARADVMLRADGKPFLLEMNTSPGMTGHSLVPMAARAAGISYEDFVMQVVAAATLDLHP
NEHWKPE
>Mature_326_residues
SFVANPKIDPKSLGKVGVLYGGRSAEREISLMSGSGVLAALQSRGVDAHGFDPATQSVAELAAAGFDRVFIALHGRYGED
GTMQGLLEQLGVPYTGSGVLASALAMDKQATKRLWMTHDLSTPRFAMLYADTDFDAVVADLGLPLIVKPAREGSSIGLSK
VTDASQMREAFEKAAALDNDVIAETFIDGAELTCPLVGEGATAEALPVIRIVAPDSNYDYQNKYFTDDTQYLCPSGLSAE
VEREVQQLAVQAYRVLGCRGWARADVMLRADGKPFLLEMNTSPGMTGHSLVPMAARAAGISYEDFVMQVVAAATLDLHPN
EHWKPE

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=306, Percent_Identity=50.3267973856209, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI1786579, Length=347, Percent_Identity=34.2939481268012, Blast_Score=182, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 34991; Mature: 34860

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFVANPKIDPKSLGKVGVLYGGRSAEREISLMSGSGVLAALQSRGVDAHGFDPATQSVA
CCCCCCCCCCHHHCCCEEEEECCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCHHHHHHH
ELAAAGFDRVFIALHGRYGEDGTMQGLLEQLGVPYTGSGVLASALAMDKQATKRLWMTHD
HHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEEEC
LSTPRFAMLYADTDFDAVVADLGLPLIVKPAREGSSIGLSKVTDASQMREAFEKAAALDN
CCCCEEEEEEECCCHHHHHHHCCCCEEEECCCCCCCCCCHHHCCHHHHHHHHHHHHHCCC
DVIAETFIDGAELTCPLVGEGATAEALPVIRIVAPDSNYDYQNKYFTDDTQYLCPSGLSA
HHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCEECCCCCEECCCCCCH
EVEREVQQLAVQAYRVLGCRGWARADVMLRADGKPFLLEMNTSPGMTGHSLVPMAARAAG
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHCC
ISYEDFVMQVVAAATLDLHPNEHWKPE
CCHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SFVANPKIDPKSLGKVGVLYGGRSAEREISLMSGSGVLAALQSRGVDAHGFDPATQSVA
CCCCCCCCCHHHCCCEEEEECCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCHHHHHHH
ELAAAGFDRVFIALHGRYGEDGTMQGLLEQLGVPYTGSGVLASALAMDKQATKRLWMTHD
HHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEEEC
LSTPRFAMLYADTDFDAVVADLGLPLIVKPAREGSSIGLSKVTDASQMREAFEKAAALDN
CCCCEEEEEEECCCHHHHHHHCCCCEEEECCCCCCCCCCHHHCCHHHHHHHHHHHHHCCC
DVIAETFIDGAELTCPLVGEGATAEALPVIRIVAPDSNYDYQNKYFTDDTQYLCPSGLSA
HHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCEECCCCCEECCCCCCH
EVEREVQQLAVQAYRVLGCRGWARADVMLRADGKPFLLEMNTSPGMTGHSLVPMAARAAG
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHCC
ISYEDFVMQVVAAATLDLHPNEHWKPE
CCHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA