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The map label for this gene is ygfG [C]

Identifier: 73542591

GI number: 73542591

Start: 3182844

End: 3183659

Strand: Reverse

Name: ygfG [C]

Synonym: Reut_A2907

Alternate gene names: 73542591

Gene position: 3183659-3182844 (Counterclockwise)

Preceding gene: 73542592

Following gene: 73542586

Centisome position: 83.64

GC content: 67.03

Gene sequence:

>816_bases
ATGAGCACCGCACGGTCTCCGGAAGGCGACGTCCCTGCCGAGGGGCGCGTCACGCTGACAATGCAGGGACCAGTCGCGAC
CCTGACATTCGACCGCCCCGCGGCACGCAATGCGATGACGTGGGCGATGTACGAGCAACTCGCCACGCACTGTCGCGCGC
TCTCTGCCCATGGCAATGCTGGCGCGCGCGTAGTGGTACTGCGAGGCGCAGGCGGCGAGGCCTTTGTTGCGGGCACGGAC
ATCGCCCAATTCCAGCAGTTCTCGGACGGCGATGACGGGGTTGCCTACGAGGCGCGCATCGACGAAGGCATCCAACTGGT
CGAGCAGTTGCCGATGCCGACAGTCGCGGTGATTGAAGGCTGGGCGGTTGGCGGTGGACTGGCCATCGCCACGGCCTGTG
ATTTCCGGGTGGCGACGCCCAGAGCCCGCTTTGGCGTGCCGATCGCAAAGACGCTCGGCAATACATTGTCGGCGCAGAAC
CTTGCCAAGCTGCGCGCGGCGTGGGGCCTCCAGCCGGTGCGGCGCATGCTGCTGCTGGCGCAGATACTCGATGCCGAGGC
GGCGCTGGTGTGTGGCTTTCTGGAAGGCGTGTATCCGGCCGAGACGCTGGATGCAGAGGTGGCCGCGCTATGCGACCGCC
TTGGCGCGCTGGCGCCCGTCACGCAGAGCGTCGTCAAGGAGGCGCTGCGCAGGCAGACCGTCGAGTCGGTGCCCGACACC
GATGATCTGGTGCGCCGCTGCTACGGCAGCAATGACTTCCATGAGGGCGTCGATGCGTTCGTGAATCGCCGGCCACCGAA
GTGGCAGGGACGCTGA

Upstream 100 bases:

>100_bases
CGCCGCCGTTGCTGGGTCAGCATACGGAGGAAGTCCTGGCGGAGCTTGGCCTTTCGAAGGATCAACAGCAGTCGCTGGCG
GCAGCCGGCGCGTTTGGCGC

Downstream 100 bases:

>100_bases
ACGGCGGTCGTGCGCGCTCAGGCGTCAGGCATGATTCCCTGCATTGACGTCGCCGCCAATTCCCAGCCGGCGCACCAGCG
GCCGCAGCGTCAGCCCGTTG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSTARSPEGDVPAEGRVTLTMQGPVATLTFDRPAARNAMTWAMYEQLATHCRALSAHGNAGARVVVLRGAGGEAFVAGTD
IAQFQQFSDGDDGVAYEARIDEGIQLVEQLPMPTVAVIEGWAVGGGLAIATACDFRVATPRARFGVPIAKTLGNTLSAQN
LAKLRAAWGLQPVRRMLLLAQILDAEAALVCGFLEGVYPAETLDAEVAALCDRLGALAPVTQSVVKEALRRQTVESVPDT
DDLVRRCYGSNDFHEGVDAFVNRRPPKWQGR

Sequences:

>Translated_271_residues
MSTARSPEGDVPAEGRVTLTMQGPVATLTFDRPAARNAMTWAMYEQLATHCRALSAHGNAGARVVVLRGAGGEAFVAGTD
IAQFQQFSDGDDGVAYEARIDEGIQLVEQLPMPTVAVIEGWAVGGGLAIATACDFRVATPRARFGVPIAKTLGNTLSAQN
LAKLRAAWGLQPVRRMLLLAQILDAEAALVCGFLEGVYPAETLDAEVAALCDRLGALAPVTQSVVKEALRRQTVESVPDT
DDLVRRCYGSNDFHEGVDAFVNRRPPKWQGR
>Mature_270_residues
STARSPEGDVPAEGRVTLTMQGPVATLTFDRPAARNAMTWAMYEQLATHCRALSAHGNAGARVVVLRGAGGEAFVAGTDI
AQFQQFSDGDDGVAYEARIDEGIQLVEQLPMPTVAVIEGWAVGGGLAIATACDFRVATPRARFGVPIAKTLGNTLSAQNL
AKLRAAWGLQPVRRMLLLAQILDAEAALVCGFLEGVYPAETLDAEVAALCDRLGALAPVTQSVVKEALRRQTVESVPDTD
DLVRRCYGSNDFHEGVDAFVNRRPPKWQGR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=249, Percent_Identity=26.1044176706827, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI157694516, Length=176, Percent_Identity=27.8409090909091, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI213417737, Length=176, Percent_Identity=27.8409090909091, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI45643119, Length=243, Percent_Identity=26.7489711934156, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI260275230, Length=243, Percent_Identity=26.7489711934156, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI260274832, Length=243, Percent_Identity=26.7489711934156, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI31542718, Length=252, Percent_Identity=22.6190476190476, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI4502327, Length=254, Percent_Identity=25.5905511811024, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI70995211, Length=197, Percent_Identity=29.9492385786802, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI87082183, Length=263, Percent_Identity=25.0950570342205, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787659, Length=262, Percent_Identity=28.6259541984733, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI221142681, Length=265, Percent_Identity=28.3018867924528, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788597, Length=254, Percent_Identity=25.5905511811024, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1790281, Length=186, Percent_Identity=27.4193548387097, Blast_Score=64, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI25145438, Length=259, Percent_Identity=27.027027027027, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17554946, Length=252, Percent_Identity=25.3968253968254, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17540714, Length=258, Percent_Identity=23.6434108527132, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=210, Percent_Identity=25.2380952380952, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=271, Percent_Identity=23.6162361623616, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17540306, Length=237, Percent_Identity=24.8945147679325, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI20129971, Length=251, Percent_Identity=27.8884462151394, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24653477, Length=251, Percent_Identity=27.8884462151394, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24653139, Length=263, Percent_Identity=26.2357414448669, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19922422, Length=257, Percent_Identity=23.3463035019455, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28917; Mature: 28786

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTARSPEGDVPAEGRVTLTMQGPVATLTFDRPAARNAMTWAMYEQLATHCRALSAHGNA
CCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
GARVVVLRGAGGEAFVAGTDIAQFQQFSDGDDGVAYEARIDEGIQLVEQLPMPTVAVIEG
CCEEEEEECCCCCEEEECCCHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCHHHHHCC
WAVGGGLAIATACDFRVATPRARFGVPIAKTLGNTLSAQNLAKLRAAWGLQPVRRMLLLA
CCCCCCEEEEEECCCEECCCCHHHCCCHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHH
QILDAEAALVCGFLEGVYPAETLDAEVAALCDRLGALAPVTQSVVKEALRRQTVESVPDT
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCH
DDLVRRCYGSNDFHEGVDAFVNRRPPKWQGR
HHHHHHHCCCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
STARSPEGDVPAEGRVTLTMQGPVATLTFDRPAARNAMTWAMYEQLATHCRALSAHGNA
CCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
GARVVVLRGAGGEAFVAGTDIAQFQQFSDGDDGVAYEARIDEGIQLVEQLPMPTVAVIEG
CCEEEEEECCCCCEEEECCCHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCHHHHHCC
WAVGGGLAIATACDFRVATPRARFGVPIAKTLGNTLSAQNLAKLRAAWGLQPVRRMLLLA
CCCCCCEEEEEECCCEECCCCHHHCCCHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHH
QILDAEAALVCGFLEGVYPAETLDAEVAALCDRLGALAPVTQSVVKEALRRQTVESVPDT
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCH
DDLVRRCYGSNDFHEGVDAFVNRRPPKWQGR
HHHHHHHCCCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA