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The map label for this gene is glcF [H]

Identifier: 73542478

GI number: 73542478

Start: 3063901

End: 3065154

Strand: Direct

Name: glcF [H]

Synonym: Reut_A2793

Alternate gene names: 73542478

Gene position: 3063901-3065154 (Clockwise)

Preceding gene: 73542477

Following gene: 73542479

Centisome position: 80.49

GC content: 66.75

Gene sequence:

>1254_bases
ATGCAAACGACCCTGGCCAATTTTCTCCGCAATACGCCTGAAGGCGAGGAAGCCAAGTCTATCGTCGGCAAATGCGTGCA
CTGCGGCTTCTGCACTGCTACCTGTCCGACCTACCAGTTGCTCGGCGACGAGCTCGACGGCCCGCGCGGGCGCATCTACC
TGATGAAGCAGGTGCTGGAGGGGAATGCCGTCAGCGAAAGCACACGTCTGCACCTGGACCGCTGCCTGACGTGCCGCAAC
TGCGAATCGACCTGCCCTTCGGGCGTGCGCTACGGGCGGCTCGTGGACATCGGCCGGCAGGTGGTGGATGAACAGCTTGA
AGCGCGCGGCGTGCGTCGTCCCGCGCGCGAACGCGTGGCGCGCTGGGTGCTGCGCGAAGGCCTGACACGCCCCGCCCTGT
TTGGCACCGCGCTGAAGCTGGGGCAGATGGTGCGGCCGATGCTGCCGCCCACGCTGCGCAACAAAGTGCCCCAACTCAGC
AAGTCCGCCGAGCCCGGCCAATGGCCGCGCACCACGCATGCACGCAAGATGCTGTTGCTCGACGGTTGCGTGCAACCGGC
CATGTCGCCCAACATCAATGCCGCAACCGCGCGCGTGTTCGACCGTGTCGGCGTACAACTGATCGTGGCGCGCGAAGCGG
GCTGTTGTGGCGCCATCCGCTTCCACACCGGCGACCACGATGGCGGCCTCGACAATATGCGCCGCAATATCGATGCATGG
TGGCCGCATCTCGAAGCGGGCGCCGAGGCCATCGTCATGACCGCATCGGGCTGCGGTGCCATGGTCAAGGACTACGGCCA
CCTGCTGCGCAACGACCCCCGCTACGCCGAGCGCGCGCGCCGCGTCTCGGCACTCACGCGCGACCTGTCGGAGATCCTGC
CGGACTTTGCCGACCTGCTGCACAAGCGCGCCGGCGCGGTGCCGCGCGATGGACGCCGCGTGGCGTACCACCCGCCATGC
ACCCTTCAGCACGGCCAGCAGATCCGCGGCAAGGTCGAAGCGCTCTTGACCGGCCTCGGTGTGGAAGTCAAACTCTGCGC
TGACAGCCACCTGTGCTGCGGCTCGGCGGGCACGTATTCCGTGTTGCAGCCGGAACTGGCCTACCGCCTGCGCGACGACA
AGCTCGCCAAACTGCAGGCCACCGAGCCCGAAGCCATCGTGTCGGCCAATATCGGCTGCATCACCCACCTGCAGGGCGGC
ACTGAAACGCCGGTCGTGCACTGGATCGAACTGGTGGACAAGATGCTGGGCTGA

Upstream 100 bases:

>100_bases
GCCACCTTCGACCCGGCAGGCATCTTCAATCCGCAGCGCATGTACCCCGGCCTCTGAGCCGCGCGCATCGTTCAACGCCA
AGGCAAACCCACCCCACACC

Downstream 100 bases:

>100_bases
ACAGCCCCGGGGCGTGGTTGTCTCCCCGCTCCCGCTTGCGGGAGCGGGGATAAACAGTACAACCAAGACGCCCTCATGCT
CCAGACCTTTGCCATCCTCC

Product: glycolate oxidase iron-sulfur subunit

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MQTTLANFLRNTPEGEEAKSIVGKCVHCGFCTATCPTYQLLGDELDGPRGRIYLMKQVLEGNAVSESTRLHLDRCLTCRN
CESTCPSGVRYGRLVDIGRQVVDEQLEARGVRRPARERVARWVLREGLTRPALFGTALKLGQMVRPMLPPTLRNKVPQLS
KSAEPGQWPRTTHARKMLLLDGCVQPAMSPNINAATARVFDRVGVQLIVAREAGCCGAIRFHTGDHDGGLDNMRRNIDAW
WPHLEAGAEAIVMTASGCGAMVKDYGHLLRNDPRYAERARRVSALTRDLSEILPDFADLLHKRAGAVPRDGRRVAYHPPC
TLQHGQQIRGKVEALLTGLGVEVKLCADSHLCCGSAGTYSVLQPELAYRLRDDKLAKLQATEPEAIVSANIGCITHLQGG
TETPVVHWIELVDKMLG

Sequences:

>Translated_417_residues
MQTTLANFLRNTPEGEEAKSIVGKCVHCGFCTATCPTYQLLGDELDGPRGRIYLMKQVLEGNAVSESTRLHLDRCLTCRN
CESTCPSGVRYGRLVDIGRQVVDEQLEARGVRRPARERVARWVLREGLTRPALFGTALKLGQMVRPMLPPTLRNKVPQLS
KSAEPGQWPRTTHARKMLLLDGCVQPAMSPNINAATARVFDRVGVQLIVAREAGCCGAIRFHTGDHDGGLDNMRRNIDAW
WPHLEAGAEAIVMTASGCGAMVKDYGHLLRNDPRYAERARRVSALTRDLSEILPDFADLLHKRAGAVPRDGRRVAYHPPC
TLQHGQQIRGKVEALLTGLGVEVKLCADSHLCCGSAGTYSVLQPELAYRLRDDKLAKLQATEPEAIVSANIGCITHLQGG
TETPVVHWIELVDKMLG
>Mature_417_residues
MQTTLANFLRNTPEGEEAKSIVGKCVHCGFCTATCPTYQLLGDELDGPRGRIYLMKQVLEGNAVSESTRLHLDRCLTCRN
CESTCPSGVRYGRLVDIGRQVVDEQLEARGVRRPARERVARWVLREGLTRPALFGTALKLGQMVRPMLPPTLRNKVPQLS
KSAEPGQWPRTTHARKMLLLDGCVQPAMSPNINAATARVFDRVGVQLIVAREAGCCGAIRFHTGDHDGGLDNMRRNIDAW
WPHLEAGAEAIVMTASGCGAMVKDYGHLLRNDPRYAERARRVSALTRDLSEILPDFADLLHKRAGAVPRDGRRVAYHPPC
TLQHGQQIRGKVEALLTGLGVEVKLCADSHLCCGSAGTYSVLQPELAYRLRDDKLAKLQATEPEAIVSANIGCITHLQGG
TETPVVHWIELVDKMLG

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=416, Percent_Identity=54.8076923076923, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI1786497, Length=236, Percent_Identity=26.271186440678, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788576, Length=359, Percent_Identity=23.6768802228412, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: 1.1.3.15

Molecular weight: Translated: 45832; Mature: 45832

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTTLANFLRNTPEGEEAKSIVGKCVHCGFCTATCPTYQLLGDELDGPRGRIYLMKQVLE
CCCHHHHHHHCCCCCHHHHHHHHHHHHCCCHHCCCCHHHHHCCCCCCCCCHHHHHHHHHC
GNAVSESTRLHLDRCLTCRNCESTCPSGVRYGRLVDIGRQVVDEQLEARGVRRPARERVA
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
RWVLREGLTRPALFGTALKLGQMVRPMLPPTLRNKVPQLSKSAEPGQWPRTTHARKMLLL
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHH
DGCVQPAMSPNINAATARVFDRVGVQLIVAREAGCCGAIRFHTGDHDGGLDNMRRNIDAW
HHHHCCCCCCCCCHHHHHHHHHCCEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHH
WPHLEAGAEAIVMTASGCGAMVKDYGHLLRNDPRYAERARRVSALTRDLSEILPDFADLL
CCCHHCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
HKRAGAVPRDGRRVAYHPPCTLQHGQQIRGKVEALLTGLGVEVKLCADSHLCCGSAGTYS
HHHCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEECCCCCCHH
VLQPELAYRLRDDKLAKLQATEPEAIVSANIGCITHLQGGTETPVVHWIELVDKMLG
HHCHHHHHHHCCCHHHHHCCCCCCHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHC
>Mature Secondary Structure
MQTTLANFLRNTPEGEEAKSIVGKCVHCGFCTATCPTYQLLGDELDGPRGRIYLMKQVLE
CCCHHHHHHHCCCCCHHHHHHHHHHHHCCCHHCCCCHHHHHCCCCCCCCCHHHHHHHHHC
GNAVSESTRLHLDRCLTCRNCESTCPSGVRYGRLVDIGRQVVDEQLEARGVRRPARERVA
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
RWVLREGLTRPALFGTALKLGQMVRPMLPPTLRNKVPQLSKSAEPGQWPRTTHARKMLLL
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHH
DGCVQPAMSPNINAATARVFDRVGVQLIVAREAGCCGAIRFHTGDHDGGLDNMRRNIDAW
HHHHCCCCCCCCCHHHHHHHHHCCEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHH
WPHLEAGAEAIVMTASGCGAMVKDYGHLLRNDPRYAERARRVSALTRDLSEILPDFADLL
CCCHHCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
HKRAGAVPRDGRRVAYHPPCTLQHGQQIRGKVEALLTGLGVEVKLCADSHLCCGSAGTYS
HHHCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEECCCCCCHH
VLQPELAYRLRDDKLAKLQATEPEAIVSANIGCITHLQGGTETPVVHWIELVDKMLG
HHCHHHHHHHCCCHHHHHCCCCCCHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]