Click here to switch to the map view.

The map label for this gene is glcE [H]

Identifier: 73542477

GI number: 73542477

Start: 3062754

End: 3063857

Strand: Direct

Name: glcE [H]

Synonym: Reut_A2792

Alternate gene names: 73542477

Gene position: 3062754-3063857 (Clockwise)

Preceding gene: 73542476

Following gene: 73542478

Centisome position: 80.46

GC content: 70.29

Gene sequence:

>1104_bases
ATGCAAGCCACTCTCGACGCCCTCCGCGATGCCGTACGGCATGCCTCCGAGACGCGCACGCCGCTGCGCCTGCGCGGCGG
CGGCAGCAAGGACTTCTATGGCAATGCCATGGACGGCGCACTGCTCGATACGCGCGCGTACCGCGGCATCGTCGAATACG
ATCCTGCCGAGCTGGTGGTCACCGCACGCTGCGGCACGCCGCTGGCCGAGATCGAGGCCGCGCTCGCGGAGAAGCGCCAG
ATGCTGGCATTCGAGCCGCCGCATTTTGCCGCCCCGGGCCAAACGAGCAGCGCGACACTCGGCGGTGCCGTGGCCGCGGG
CCTGTCCGGTCCGCGCCGGCAATCGGTCGGTGCGCTGCGCGACTTCGTACTGGGCGCGCAGATGATGGATGGCCACGGCG
AGGTCATGAGCTTTGGCGGCCAGGTCATGAAGAACGTCGCGGGCTATGACGTGTCGCGGCTGCTGGCGGGATCGCTCGGC
ACGCTGGGGTTGATTCTCGAAGTCTCGCTCAAGGTGCTGCCGACGCCGTTCGACGACGCCACGCTGTGCTTTGCGATGAG
CCAGGCCGAGGCCATCGACCGCATTAACCAGTGGGGCGGCCAGCCGCTGCCGATCGCGGCTTCCGCATGGCATGAAGGCG
TGTTGCACGTGCGGCTGTCGGGCGCCACGGCCGCAGTGCGAGCCGCACGCGCGCGGCTCGGCGGCGATGCCGTCGCGCAG
GACGAAGGCGTGGTCTTCTGGCAGTCGCTGCGCGAACAGACGCATGCGTTCTTCGCGCCGGCGCTGCAGGGCCGCGCGCT
GTGGCGCCTGGCGGTGCCGCCGACATCGGCGCCGCTGGATCTGCCCGGCTCACAGCTGGTCGAATGGGGCGGCGGGCAGC
GCTGGTGGCTGCCCGATGCGGGCCCGGACGGCAACCATGCCAGGCTCGTGCGGGGAACCGCGCAGGCCGTCGGCGGACAT
GCCACGCTGTTCCGCAACGGCGACAAGTCGGTCGGCGTGTTCACACCACTGGCCGCACCGCTGGCCGCGATCCATGCACG
GCTGAAGGCCACCTTCGACCCGGCAGGCATCTTCAATCCGCAGCGCATGTACCCCGGCCTCTGA

Upstream 100 bases:

>100_bases
AACGACCGCCCAGAAGTACCGGGCGGGCCCGCCGGCGGTCGCCGGAAAGACGCCTGACCCACGGGCAATACGTACCCGCA
CGATAAAGAACCGGACCCAC

Downstream 100 bases:

>100_bases
GCCGCGCGCATCGTTCAACGCCAAGGCAAACCCACCCCACACCATGCAAACGACCCTGGCCAATTTTCTCCGCAATACGC
CTGAAGGCGAGGAAGCCAAG

Product: glycolate oxidase FAD binding subunit

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MQATLDALRDAVRHASETRTPLRLRGGGSKDFYGNAMDGALLDTRAYRGIVEYDPAELVVTARCGTPLAEIEAALAEKRQ
MLAFEPPHFAAPGQTSSATLGGAVAAGLSGPRRQSVGALRDFVLGAQMMDGHGEVMSFGGQVMKNVAGYDVSRLLAGSLG
TLGLILEVSLKVLPTPFDDATLCFAMSQAEAIDRINQWGGQPLPIAASAWHEGVLHVRLSGATAAVRAARARLGGDAVAQ
DEGVVFWQSLREQTHAFFAPALQGRALWRLAVPPTSAPLDLPGSQLVEWGGGQRWWLPDAGPDGNHARLVRGTAQAVGGH
ATLFRNGDKSVGVFTPLAAPLAAIHARLKATFDPAGIFNPQRMYPGL

Sequences:

>Translated_367_residues
MQATLDALRDAVRHASETRTPLRLRGGGSKDFYGNAMDGALLDTRAYRGIVEYDPAELVVTARCGTPLAEIEAALAEKRQ
MLAFEPPHFAAPGQTSSATLGGAVAAGLSGPRRQSVGALRDFVLGAQMMDGHGEVMSFGGQVMKNVAGYDVSRLLAGSLG
TLGLILEVSLKVLPTPFDDATLCFAMSQAEAIDRINQWGGQPLPIAASAWHEGVLHVRLSGATAAVRAARARLGGDAVAQ
DEGVVFWQSLREQTHAFFAPALQGRALWRLAVPPTSAPLDLPGSQLVEWGGGQRWWLPDAGPDGNHARLVRGTAQAVGGH
ATLFRNGDKSVGVFTPLAAPLAAIHARLKATFDPAGIFNPQRMYPGL
>Mature_367_residues
MQATLDALRDAVRHASETRTPLRLRGGGSKDFYGNAMDGALLDTRAYRGIVEYDPAELVVTARCGTPLAEIEAALAEKRQ
MLAFEPPHFAAPGQTSSATLGGAVAAGLSGPRRQSVGALRDFVLGAQMMDGHGEVMSFGGQVMKNVAGYDVSRLLAGSLG
TLGLILEVSLKVLPTPFDDATLCFAMSQAEAIDRINQWGGQPLPIAASAWHEGVLHVRLSGATAAVRAARARLGGDAVAQ
DEGVVFWQSLREQTHAFFAPALQGRALWRLAVPPTSAPLDLPGSQLVEWGGGQRWWLPDAGPDGNHARLVRGTAQAVGGH
ATLFRNGDKSVGVFTPLAAPLAAIHARLKATFDPAGIFNPQRMYPGL

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Escherichia coli, GI48994914, Length=362, Percent_Identity=48.3425414364641, Blast_Score=313, Evalue=1e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 38725; Mature: 38725

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQATLDALRDAVRHASETRTPLRLRGGGSKDFYGNAMDGALLDTRAYRGIVEYDPAELVV
CCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCEEECCCCEEE
TARCGTPLAEIEAALAEKRQMLAFEPPHFAAPGQTSSATLGGAVAAGLSGPRRQSVGALR
EECCCCCHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHH
DFVLGAQMMDGHGEVMSFGGQVMKNVAGYDVSRLLAGSLGTLGLILEVSLKVLPTPFDDA
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHEEHHHCCCCCCCCC
TLCFAMSQAEAIDRINQWGGQPLPIAASAWHEGVLHVRLSGATAAVRAARARLGGDAVAQ
HHHHHHHHHHHHHHHHHCCCCCCCEEHHHHCCCEEEEEECCHHHHHHHHHHHCCCCCCCC
DEGVVFWQSLREQTHAFFAPALQGRALWRLAVPPTSAPLDLPGSQLVEWGGGQRWWLPDA
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHCCCCCEEECCCC
GPDGNHARLVRGTAQAVGGHATLFRNGDKSVGVFTPLAAPLAAIHARLKATFDPAGIFNP
CCCCCCEEEEECHHHHCCCCEEEEECCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCC
QRMYPGL
HHCCCCC
>Mature Secondary Structure
MQATLDALRDAVRHASETRTPLRLRGGGSKDFYGNAMDGALLDTRAYRGIVEYDPAELVV
CCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCEEECCCCEEE
TARCGTPLAEIEAALAEKRQMLAFEPPHFAAPGQTSSATLGGAVAAGLSGPRRQSVGALR
EECCCCCHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHH
DFVLGAQMMDGHGEVMSFGGQVMKNVAGYDVSRLLAGSLGTLGLILEVSLKVLPTPFDDA
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHEEHHHCCCCCCCCC
TLCFAMSQAEAIDRINQWGGQPLPIAASAWHEGVLHVRLSGATAAVRAARARLGGDAVAQ
HHHHHHHHHHHHHHHHHCCCCCCCEEHHHHCCCEEEEEECCHHHHHHHHHHHCCCCCCCC
DEGVVFWQSLREQTHAFFAPALQGRALWRLAVPPTSAPLDLPGSQLVEWGGGQRWWLPDA
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHCCCCCEEECCCC
GPDGNHARLVRGTAQAVGGHATLFRNGDKSVGVFTPLAAPLAAIHARLKATFDPAGIFNP
CCCCCCEEEEECHHHHCCCCEEEEECCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCC
QRMYPGL
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]