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The map label for this gene is folK [H]

Identifier: 73542463

GI number: 73542463

Start: 3047365

End: 3047901

Strand: Direct

Name: folK [H]

Synonym: Reut_A2778

Alternate gene names: 73542463

Gene position: 3047365-3047901 (Clockwise)

Preceding gene: 73542462

Following gene: 73542464

Centisome position: 80.06

GC content: 64.99

Gene sequence:

>537_bases
ATGACACTCGCCTTCATCGGCATTGGTGCCAACCTGGGCGATGCCCGCCAGGCACTGAAAGACGCGATCGTATGCCTGGC
TCAGCAGGTCGGCATTACGGTACTGGCACGGTCTTCGCTGTATCGGACCGCGCCCGTCGATGCCGATGGCGACGATTACT
ACAACGCGGTGGTCAAGGTGCAGACCTCGTTCACGGCCGCGCAGCTGCTTCGCATCTGCCACCACATCGAAGACCAGTTT
GGCCGCGAACGCCCGTTCCGCAACGCGCCGCGCACGCTGGACCTGGACCTGCTTGTGTTCGGTGACGAACAGCACAACCA
CGAGCATCTGACGGTACCGCATCCGCGCGTCACCGAGCGCGCCTTTACGCTGGTGCCGCTGCTGGAGCTGGCCCCCGAAC
TGGTGATTCCCGGCGTCGGCCCCGCCGTCGACTTTCTCGCTCGGGTTGGCGGCCAGCGCATCGATAAAGTCTCCACCTGC
AAGTGCATGCGCGCGCAGGCCGCCGGTTCAGCCGGCCCCTCCGACGCTAAGGACTGA

Upstream 100 bases:

>100_bases
GCCGACGCGGTCCGCGGAAATCGGATAAAGTTTCTTCCCGGAGCGACACGGACGCGGGGCAGGAATCACATGCCGGCCGC
GGCCAGCCGGAGGAACATCA

Downstream 100 bases:

>100_bases
GCCGGCGGAGCCATCCGCCTCGCGCCGGCAACGATATGCTCGAACACCTGCGCCGCATCGTCGTGGAAGGCCCGGTCGGG
TCCGGCAAGACTTCGCTGGC

Product: 7, 8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 178; Mature: 177

Protein sequence:

>178_residues
MTLAFIGIGANLGDARQALKDAIVCLAQQVGITVLARSSLYRTAPVDADGDDYYNAVVKVQTSFTAAQLLRICHHIEDQF
GRERPFRNAPRTLDLDLLVFGDEQHNHEHLTVPHPRVTERAFTLVPLLELAPELVIPGVGPAVDFLARVGGQRIDKVSTC
KCMRAQAAGSAGPSDAKD

Sequences:

>Translated_178_residues
MTLAFIGIGANLGDARQALKDAIVCLAQQVGITVLARSSLYRTAPVDADGDDYYNAVVKVQTSFTAAQLLRICHHIEDQF
GRERPFRNAPRTLDLDLLVFGDEQHNHEHLTVPHPRVTERAFTLVPLLELAPELVIPGVGPAVDFLARVGGQRIDKVSTC
KCMRAQAAGSAGPSDAKD
>Mature_177_residues
TLAFIGIGANLGDARQALKDAIVCLAQQVGITVLARSSLYRTAPVDADGDDYYNAVVKVQTSFTAAQLLRICHHIEDQFG
RERPFRNAPRTLDLDLLVFGDEQHNHEHLTVPHPRVTERAFTLVPLLELAPELVIPGVGPAVDFLARVGGQRIDKVSTCK
CMRAQAAGSAGPSDAKD

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=139, Percent_Identity=45.3237410071942, Blast_Score=108, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324073, Length=162, Percent_Identity=32.7160493827161, Blast_Score=88, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 19283; Mature: 19152

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS00794 HPPK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLAFIGIGANLGDARQALKDAIVCLAQQVGITVLARSSLYRTAPVDADGDDYYNAVVKV
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHCHHEEECCCHHCCCCCCCCCCHHEEEEEEE
QTSFTAAQLLRICHHIEDQFGRERPFRNAPRTLDLDLLVFGDEQHNHEHLTVPHPRVTER
ECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCCCEEECCCCCHHHH
AFTLVPLLELAPELVIPGVGPAVDFLARVGGQRIDKVSTCKCMRAQAAGSAGPSDAKD
HHHHHHHHHHCHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TLAFIGIGANLGDARQALKDAIVCLAQQVGITVLARSSLYRTAPVDADGDDYYNAVVKV
EEEEEEECCCCCHHHHHHHHHHHHHHHHHCHHEEECCCHHCCCCCCCCCCHHEEEEEEE
QTSFTAAQLLRICHHIEDQFGRERPFRNAPRTLDLDLLVFGDEQHNHEHLTVPHPRVTER
ECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCCCEEECCCCCHHHH
AFTLVPLLELAPELVIPGVGPAVDFLARVGGQRIDKVSTCKCMRAQAAGSAGPSDAKD
HHHHHHHHHHCHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9168622 [H]