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The map label for this gene is pcnB [H]

Identifier: 73542462

GI number: 73542462

Start: 3045839

End: 3047368

Strand: Direct

Name: pcnB [H]

Synonym: Reut_A2777

Alternate gene names: 73542462

Gene position: 3045839-3047368 (Clockwise)

Preceding gene: 73542461

Following gene: 73542463

Centisome position: 80.02

GC content: 67.32

Gene sequence:

>1530_bases
GTGATCAAAAAGCTCATCAACCGGCTACTCGGCAAGCCCGGCCCCAAGCAGCGCCGCATTGGCCGCGCCCACACGCCGCG
CATCGTCACCGTGGAAGAGCACCGCATCGACCCGACGCTGCTGTCGCGCAATGCGGTCAAAGTCACTTCCACGCTGCAAC
AGGCGGGGTACCAGGCCTTTATCGTCGGCGGCGCTGTGCGGGACCTGCTGCTTGGCATCAAGCCGAAGGATTTCGACGTC
GCCACCAACGCCACGCCCGAGCAGGTGCAGTCGCTGTTCCGGCGCTCGCGCATCATCGGGCGGCGCTTCCAGATCGTCCA
CGTGACGTTCTACGGCGGGCGCGAGCAGGAAATCATCGAGGTTTCCACGTTCCGCGCGCTGGTCGATGCCGTCGCCAGCG
AGACGCTGCCAGAGGGCCGGCGCCTGAAGCGTTCGGAACTCGACAGCAAGACGCACGCGATCGACGCATCGGGACGCGTG
TTGCGCGACAACGTGTGGGGATCGCAGGCCGAAGACGCGGAACGCCGCGACTTCACCATCAATGCGATGTACTACGACCC
GGCCGCGCAGACCGTGCATGACTACCATCACGGCATGGAAGACATCCGCGCGCGCACGCTGCGCATGATCGGCGACCCGA
CCCTGCGCTACCGCGAGGATCCGATCCGCATGCTGCGCGTGGTGCGCTTTGCGGCCAAGACCGGCTTCGGCATCGACGAG
GCCACGCGCGAGCCGATCGGCGGCCTGGCGTCGCTGATCCACAACGTGCCGAGCGCGCGCCTGTTCGACGAAATGCTCAA
GCTGCTGATGTCCGGCCATGCGTGGGCATCCCTACAGGAACTGCGCAAGGCCGGCCTGCACAAAGGCCTGCTGCCGCTGC
TCGACGTGGCGCTGGAACAGCCGATGGGCCAGCGCTTCGTGCAGCTCGCGCTCGACAACACCGACCGGCGCGTACAGGCC
GGCAAGCCGGTCTCGCCGGGCTTCCTGTTCGCGGCGCTGCTGTGGCACCACGTGCTGCAGCGCTGGAACGCCGAGCGCGA
ACGCGGCGAGCATCCGATCGCTGCGCTCAATATCGCCATGGATGCCGTGCTCGAAAAGCAGACCGGCCAGCTTGCGATCC
AGCGCCGCTTCGTCACCGACATGCGTGACATCTGGGGCATGCAGCCGCGCTTCGAAAAGCGCGTGGGACGCATGCCGTTC
CGCCTGCTGGAGTCGCCGCGCTTCCGCGCCGGCTTCGACTTCCTGCACCTGCGCTGCCAGTCCGGTGAAATGCCCGAGGA
GATTTCCCGCTGGTGGCAGGACTTCCAGAATGCCGACCCGGATGAGCGCGAAGGCCTGATCGACGCCGCGCGCACCCCGC
GTGGCCCGGCCGGCCAGGCCGGTCCTGCTGGCACCGAAGGCGAAGGCCCGGCGCGCAAGAAGCGCCGCCGACGCGGTCCG
CGGAAATCGGATAAAGTTTCTTCCCGGAGCGACACGGACGCGGGGCAGGAATCACATGCCGGCCGCGGCCAGCCGGAGGA
ACATCAATGA

Upstream 100 bases:

>100_bases
CTGATGGAAAAGTCGAGCGAACCGATCGCCACCAACCCGGACGATCGCCTGCGCGAGCATGCCGCCGCTGCGGGCTGGCG
CATCATGGACCTGTTCTGAC

Downstream 100 bases:

>100_bases
CACTCGCCTTCATCGGCATTGGTGCCAACCTGGGCGATGCCCGCCAGGCACTGAAAGACGCGATCGTATGCCTGGCTCAG
CAGGTCGGCATTACGGTACT

Product: Poly(A) polymerase, PcnB

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MIKKLINRLLGKPGPKQRRIGRAHTPRIVTVEEHRIDPTLLSRNAVKVTSTLQQAGYQAFIVGGAVRDLLLGIKPKDFDV
ATNATPEQVQSLFRRSRIIGRRFQIVHVTFYGGREQEIIEVSTFRALVDAVASETLPEGRRLKRSELDSKTHAIDASGRV
LRDNVWGSQAEDAERRDFTINAMYYDPAAQTVHDYHHGMEDIRARTLRMIGDPTLRYREDPIRMLRVVRFAAKTGFGIDE
ATREPIGGLASLIHNVPSARLFDEMLKLLMSGHAWASLQELRKAGLHKGLLPLLDVALEQPMGQRFVQLALDNTDRRVQA
GKPVSPGFLFAALLWHHVLQRWNAERERGEHPIAALNIAMDAVLEKQTGQLAIQRRFVTDMRDIWGMQPRFEKRVGRMPF
RLLESPRFRAGFDFLHLRCQSGEMPEEISRWWQDFQNADPDEREGLIDAARTPRGPAGQAGPAGTEGEGPARKKRRRRGP
RKSDKVSSRSDTDAGQESHAGRGQPEEHQ

Sequences:

>Translated_509_residues
MIKKLINRLLGKPGPKQRRIGRAHTPRIVTVEEHRIDPTLLSRNAVKVTSTLQQAGYQAFIVGGAVRDLLLGIKPKDFDV
ATNATPEQVQSLFRRSRIIGRRFQIVHVTFYGGREQEIIEVSTFRALVDAVASETLPEGRRLKRSELDSKTHAIDASGRV
LRDNVWGSQAEDAERRDFTINAMYYDPAAQTVHDYHHGMEDIRARTLRMIGDPTLRYREDPIRMLRVVRFAAKTGFGIDE
ATREPIGGLASLIHNVPSARLFDEMLKLLMSGHAWASLQELRKAGLHKGLLPLLDVALEQPMGQRFVQLALDNTDRRVQA
GKPVSPGFLFAALLWHHVLQRWNAERERGEHPIAALNIAMDAVLEKQTGQLAIQRRFVTDMRDIWGMQPRFEKRVGRMPF
RLLESPRFRAGFDFLHLRCQSGEMPEEISRWWQDFQNADPDEREGLIDAARTPRGPAGQAGPAGTEGEGPARKKRRRRGP
RKSDKVSSRSDTDAGQESHAGRGQPEEHQ
>Mature_509_residues
MIKKLINRLLGKPGPKQRRIGRAHTPRIVTVEEHRIDPTLLSRNAVKVTSTLQQAGYQAFIVGGAVRDLLLGIKPKDFDV
ATNATPEQVQSLFRRSRIIGRRFQIVHVTFYGGREQEIIEVSTFRALVDAVASETLPEGRRLKRSELDSKTHAIDASGRV
LRDNVWGSQAEDAERRDFTINAMYYDPAAQTVHDYHHGMEDIRARTLRMIGDPTLRYREDPIRMLRVVRFAAKTGFGIDE
ATREPIGGLASLIHNVPSARLFDEMLKLLMSGHAWASLQELRKAGLHKGLLPLLDVALEQPMGQRFVQLALDNTDRRVQA
GKPVSPGFLFAALLWHHVLQRWNAERERGEHPIAALNIAMDAVLEKQTGQLAIQRRFVTDMRDIWGMQPRFEKRVGRMPF
RLLESPRFRAGFDFLHLRCQSGEMPEEISRWWQDFQNADPDEREGLIDAARTPRGPAGQAGPAGTEGEGPARKKRRRRGP
RKSDKVSSRSDTDAGQESHAGRGQPEEHQ

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=242, Percent_Identity=31.404958677686, Blast_Score=96, Evalue=9e-20,
Organism=Escherichia coli, GI87081691, Length=432, Percent_Identity=43.287037037037, Blast_Score=337, Evalue=1e-93,
Organism=Caenorhabditis elegans, GI71995920, Length=338, Percent_Identity=26.9230769230769, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 57605; Mature: 57605

Theoretical pI: Translated: 10.66; Mature: 10.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKLINRLLGKPGPKQRRIGRAHTPRIVTVEEHRIDPTLLSRNAVKVTSTLQQAGYQAF
CHHHHHHHHHCCCCCHHHHCCCCCCCEEEEECCCCCCHHHHCCCHHHHHHHHHHCCCEEE
IVGGAVRDLLLGIKPKDFDVATNATPEQVQSLFRRSRIIGRRFQIVHVTFYGGREQEIIE
EEHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCHHHH
VSTFRALVDAVASETLPEGRRLKRSELDSKTHAIDASGRVLRDNVWGSQAEDAERRDFTI
HHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHEECCCCCEEECCCCCCCCCCCCCCCEEE
NAMYYDPAAQTVHDYHHGMEDIRARTLRMIGDPTLRYREDPIRMLRVVRFAAKTGFGIDE
EEEEECCHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHCCCCCCH
ATREPIGGLASLIHNVPSARLFDEMLKLLMSGHAWASLQELRKAGLHKGLLPLLDVALEQ
HHHCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHC
PMGQRFVQLALDNTDRRVQAGKPVSPGFLFAALLWHHVLQRWNAERERGEHPIAALNIAM
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHH
DAVLEKQTGQLAIQRRFVTDMRDIWGMQPRFEKRVGRMPFRLLESPRFRAGFDFLHLRCQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHCCCCCCCCCCEEEEEEC
SGEMPEEISRWWQDFQNADPDEREGLIDAARTPRGPAGQAGPAGTEGEGPARKKRRRRGP
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCC
RKSDKVSSRSDTDAGQESHAGRGQPEEHQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIKKLINRLLGKPGPKQRRIGRAHTPRIVTVEEHRIDPTLLSRNAVKVTSTLQQAGYQAF
CHHHHHHHHHCCCCCHHHHCCCCCCCEEEEECCCCCCHHHHCCCHHHHHHHHHHCCCEEE
IVGGAVRDLLLGIKPKDFDVATNATPEQVQSLFRRSRIIGRRFQIVHVTFYGGREQEIIE
EEHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCHHHH
VSTFRALVDAVASETLPEGRRLKRSELDSKTHAIDASGRVLRDNVWGSQAEDAERRDFTI
HHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHEECCCCCEEECCCCCCCCCCCCCCCEEE
NAMYYDPAAQTVHDYHHGMEDIRARTLRMIGDPTLRYREDPIRMLRVVRFAAKTGFGIDE
EEEEECCHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHCCCCCCH
ATREPIGGLASLIHNVPSARLFDEMLKLLMSGHAWASLQELRKAGLHKGLLPLLDVALEQ
HHHCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHC
PMGQRFVQLALDNTDRRVQAGKPVSPGFLFAALLWHHVLQRWNAERERGEHPIAALNIAM
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHH
DAVLEKQTGQLAIQRRFVTDMRDIWGMQPRFEKRVGRMPFRLLESPRFRAGFDFLHLRCQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHCCCCCCCCCCEEEEEEC
SGEMPEEISRWWQDFQNADPDEREGLIDAARTPRGPAGQAGPAGTEGEGPARKKRRRRGP
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCC
RKSDKVSSRSDTDAGQESHAGRGQPEEHQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]