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The map label for this gene is miaA

Identifier: 73542455

GI number: 73542455

Start: 3038984

End: 3039940

Strand: Direct

Name: miaA

Synonym: Reut_A2770

Alternate gene names: 73542455

Gene position: 3038984-3039940 (Clockwise)

Preceding gene: 73542454

Following gene: 73542457

Centisome position: 79.84

GC content: 68.86

Gene sequence:

>957_bases
ATGTCCGCCCTGCCTTCGTCCGAACACCCGCCCGTCGTCTGCCTGCTGGGCCCGACCGCCTCAGGCAAGACCGCGGCCGC
CCTGGCCCTGGCACAGCAGGCGCCGGTCGAGATCATCAGCCTCGATTCGGCGCTGGTCTATCGCGAGATGGATATCGGCA
CTGCCAAGCCCACGCGTGAAGAGCTGGCCTCCGTGCCACACCACCTGATCGACATCATCGATCCGACCGACAGCTACTCC
GCCGCGCAATTCGTCACCGACGCCGAACGGCTGATCGCCGAGATCCGCAGTCGCGGCCACCAGCCGCTGATCGTCGGCGG
CACCATGCTGTACTACAAGGCGCTGACGCAGGGCCTGAACGACCTGCCGCAGGCTGATCCTGCCGTGCGCGCCAAACTCG
ACGAACTGGCCGCCGAACGCGGCTGGCCCGCACTGCACGCGATGCTGGCGGAGGTCGACCCGGTCACCGCCGCGCGGCTC
GCGCCCAACGACGCGCAGCGCATCCAGCGTGCGCTGGAGATCCACCGGTTATCGGGGCAACCAATGTCCGAGCTGCTGGC
GCGGCAGGCCGACGGCCGTACCTTTACCGGCGCAGCGGATCAGCGCTACCGCGTGATCGCGCTGGAGCCGTCGGACCGCG
CCGTGCTGCATGCACGCATCGCCGCGCGCTACGACGCCATGCTGGCTGGCGGCTTTATCGAGGAAGTCGAACGCCTGCGC
CGGCGCGGCGATCTCCATCCGGGCCTGCCGTCGATCCGCTGCGTAGGCTACCGTCAAGTCTGGGAATACCTGGACGGCGA
CACCGATTACGCGACCATGCGCGAACGCGGCATCGTGGCCACGCGCCAGCTCTGCAAGCGCCAGATCACGTGGCTGCGCA
GCACGCCCGAGCGGCTGTCCGTCGATTGCGTCGCACCCGACTACGTCCAGCAGGTAGCGCGGCTGGCGGGCATGTGA

Upstream 100 bases:

>100_bases
GGTTCAGCTGAGCGTGTCTGAGCTCGATCGTCTTTTCCTGCGCGGCCAGTAACACCGCGTTTTTCACCGCGTTCGCGCAC
TACGCCTTGCAGAAGTCCTG

Downstream 100 bases:

>100_bases
CCCACCCGTCGGCACTAAGCCAGCCTATTGCGGCGACGCCTTGCGCTCGCCGTGGATCTCGCCTGCCACGCGCTCGATGC
AACGCATCGCCTTCATCGCC

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSALPSSEHPPVVCLLGPTASGKTAAALALAQQAPVEIISLDSALVYREMDIGTAKPTREELASVPHHLIDIIDPTDSYS
AAQFVTDAERLIAEIRSRGHQPLIVGGTMLYYKALTQGLNDLPQADPAVRAKLDELAAERGWPALHAMLAEVDPVTAARL
APNDAQRIQRALEIHRLSGQPMSELLARQADGRTFTGAADQRYRVIALEPSDRAVLHARIAARYDAMLAGGFIEEVERLR
RRGDLHPGLPSIRCVGYRQVWEYLDGDTDYATMRERGIVATRQLCKRQITWLRSTPERLSVDCVAPDYVQQVARLAGM

Sequences:

>Translated_318_residues
MSALPSSEHPPVVCLLGPTASGKTAAALALAQQAPVEIISLDSALVYREMDIGTAKPTREELASVPHHLIDIIDPTDSYS
AAQFVTDAERLIAEIRSRGHQPLIVGGTMLYYKALTQGLNDLPQADPAVRAKLDELAAERGWPALHAMLAEVDPVTAARL
APNDAQRIQRALEIHRLSGQPMSELLARQADGRTFTGAADQRYRVIALEPSDRAVLHARIAARYDAMLAGGFIEEVERLR
RRGDLHPGLPSIRCVGYRQVWEYLDGDTDYATMRERGIVATRQLCKRQITWLRSTPERLSVDCVAPDYVQQVARLAGM
>Mature_317_residues
SALPSSEHPPVVCLLGPTASGKTAAALALAQQAPVEIISLDSALVYREMDIGTAKPTREELASVPHHLIDIIDPTDSYSA
AQFVTDAERLIAEIRSRGHQPLIVGGTMLYYKALTQGLNDLPQADPAVRAKLDELAAERGWPALHAMLAEVDPVTAARLA
PNDAQRIQRALEIHRLSGQPMSELLARQADGRTFTGAADQRYRVIALEPSDRAVLHARIAARYDAMLAGGFIEEVERLRR
RGDLHPGLPSIRCVGYRQVWEYLDGDTDYATMRERGIVATRQLCKRQITWLRSTPERLSVDCVAPDYVQQVARLAGM

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=312, Percent_Identity=32.3717948717949, Blast_Score=133, Evalue=2e-31,
Organism=Escherichia coli, GI1790613, Length=322, Percent_Identity=50.9316770186335, Blast_Score=311, Evalue=4e-86,
Organism=Caenorhabditis elegans, GI25144712, Length=278, Percent_Identity=27.3381294964029, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324848, Length=295, Percent_Identity=29.1525423728814, Blast_Score=130, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24649830, Length=273, Percent_Identity=31.1355311355311, Blast_Score=121, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_CUPPJ (Q46XK2)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_296975.1
- ProteinModelPortal:   Q46XK2
- SMR:   Q46XK2
- GeneID:   3612340
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A2770
- NMPDR:   fig|264198.3.peg.2202
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   REUT264198:REUT_A2770-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34943; Mature: 34812

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALPSSEHPPVVCLLGPTASGKTAAALALAQQAPVEIISLDSALVYREMDIGTAKPTRE
CCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCEEEEECCHHHHHEECCCCCCCCCHH
ELASVPHHLIDIIDPTDSYSAAQFVTDAERLIAEIRSRGHQPLIVGGTMLYYKALTQGLN
HHHHCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHH
DLPQADPAVRAKLDELAAERGWPALHAMLAEVDPVTAARLAPNDAQRIQRALEIHRLSGQ
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCCC
PMSELLARQADGRTFTGAADQRYRVIALEPSDRAVLHARIAARYDAMLAGGFIEEVERLR
CHHHHHHHHCCCCEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRGDLHPGLPSIRCVGYRQVWEYLDGDTDYATMRERGIVATRQLCKRQITWLRSTPERLS
HHCCCCCCCCCEEEHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCHHHCE
VDCVAPDYVQQVARLAGM
EEECCHHHHHHHHHHHCC
>Mature Secondary Structure 
SALPSSEHPPVVCLLGPTASGKTAAALALAQQAPVEIISLDSALVYREMDIGTAKPTRE
CCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCEEEEECCHHHHHEECCCCCCCCCHH
ELASVPHHLIDIIDPTDSYSAAQFVTDAERLIAEIRSRGHQPLIVGGTMLYYKALTQGLN
HHHHCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHH
DLPQADPAVRAKLDELAAERGWPALHAMLAEVDPVTAARLAPNDAQRIQRALEIHRLSGQ
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCCC
PMSELLARQADGRTFTGAADQRYRVIALEPSDRAVLHARIAARYDAMLAGGFIEEVERLR
CHHHHHHHHCCCCEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRGDLHPGLPSIRCVGYRQVWEYLDGDTDYATMRERGIVATRQLCKRQITWLRSTPERLS
HHCCCCCCCCCEEEHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCHHHCE
VDCVAPDYVQQVARLAGM
EEECCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA