Click here to switch to the map view.

The map label for this gene is nadB1 [H]

Identifier: 73542420

GI number: 73542420

Start: 3000628

End: 3002214

Strand: Direct

Name: nadB1 [H]

Synonym: Reut_A2735

Alternate gene names: 73542420

Gene position: 3000628-3002214 (Clockwise)

Preceding gene: 73542419

Following gene: 73542427

Centisome position: 78.83

GC content: 64.9

Gene sequence:

>1587_bases
ATGAACTTCGACGTCGCCATCGTAGGCAGCGGGCTGGCCGGTCTCACGGTCGCGCTGCAACTGGCCGACACCCATCGGGT
TGTCATCCTCAGCAAGCGCGCCATGACGCAGGGCGCCAGCGACTGGGCACAGGGCGGCATTGCCGCCGTGCTTGATTCGG
GCGACAGCCACGACGAGCATACGCAGGACACGCTCGTGGCCGGTGCGGGCCTCTGCGATGAAGAGGCAACGCGCTTCATC
GTCGAGCATGGACGTGAAGCGATTCAGTGGCTGATCGACCGCGGCGTGCCGTTCACCAAAGATGAGCAGGCCGAACTCGG
CTATCACCTGACACGCGAAGGCGGACATAGCCGGCGCCGCATCATCCATGCCGCGGATGCAACGGGTCACGCCGTGGTCA
GCACGCTGTCGGAGATGGCCCGCCAGCATCCGAACATCACTGTTATTGAAGACCAGTTTGCGATCGACCTGATCACGTCG
CGCAAGCTGGGGCTGCCGGGGAACCGCTGCTACGGCCTGTACGTGCTCGACGACAGCACCGGAGAAGTCCGTACCATCAC
CGCGACGCACACGGTGCTGGCGGCCGGCGGCGCAGGCAAGGTGTACCTGTACACGACCAACCCAGACACGGCCACCGGCG
ACGGCATTGCCATGGCATGGCGCGCGGGTTGCCGCGTGTCGAACATGGAATTCATCCAGTTTCATCCGACCTGCCTGTAC
CACCCGTACGCCAAGTCCTTCCTGATCTCGGAAGCCGTGCGCGGCGAAGGCGGCCTGCTCAAGCTGCCGGACGGCACCCG
CTTCATGCCCGAGCACGATGAGCGCGCAGAACTCGCGCCGCGTGACGTGGTGGCACGGGCGATCGACTTCGAAATGAAGA
AGCGCGGCCTGGACTGCGTCTATCTCGATATCACGCACCAGCCCGAGGCCTTCCTGAAGGAACATTTCCCCACGATCCAC
GCGCGCTGCCTGGAACTGGGCATCGACATCGCGCGCGAACCGATCCCCGTGGTACCGGCTGCGCATTACACCTGCGGCGG
CGTGGTGACCGACACCCTGGGCCGAACCGACATCGGCAATCTCTACGCCGTGGGCGAGACGGCATGCACCGGGCTGCACG
GCGCTAACCGTCTTGCATCGAATTCGCTGCTCGAATGCATTGTGATCGGCCGCGCGGCCGCGACCGACATCGCAAGCCAG
GACAAGGCCGGCCTGCCGGATCTCGCACTGCCGTCCTGGGATGAAAGCCGCGTCTCCGACGCCGACGAGGAAGTTGTGGT
CTCGCACAACTGGGACGAGCTGCGCCGCATGATGTGGAACTACGTCGGCATCGTGCGCACCAGCAAGCGCCTGGAACGCG
CGCAGCACCGTATCCGCCTGCTGCGCGAGGAGATCGCCGAGTACTACGCGAACTTCCGCGTCACGCGCGACCTGCTCGAG
CTGCGCAATCTCGTCGAAGTCGCCTCGCTGATCGTCGATAGCGCGTACTCCCGCCACGAAAGCCGCGGCCTGCATTTCAG
CCGGGACTATCCGGATACGTTGCCGAAGGCTCTTCCTACCGTGATGCAGCCGGCCCGCAAGCGCTAA

Upstream 100 bases:

>100_bases
CTCGCGGCATTGCAGCTCTGGTGCATCAGGGCATCACCCGGGTGTAGACTTTCGAGCTTTCCCTCTAGCGCCGCGCCCCC
GCGCGCGCCACTCCTGCACC

Downstream 100 bases:

>100_bases
AGAGAAGTCCCTGCACGCAGAAAGCAAAACGGCCCACATAAAGCGGGCCGTTTTTGTTCTGACGTAGCTGACGATCAGCC
GATGATCCGCAGCGAGTAGT

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO 1; Quinolinate synthase B 1 [H]

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MNFDVAIVGSGLAGLTVALQLADTHRVVILSKRAMTQGASDWAQGGIAAVLDSGDSHDEHTQDTLVAGAGLCDEEATRFI
VEHGREAIQWLIDRGVPFTKDEQAELGYHLTREGGHSRRRIIHAADATGHAVVSTLSEMARQHPNITVIEDQFAIDLITS
RKLGLPGNRCYGLYVLDDSTGEVRTITATHTVLAAGGAGKVYLYTTNPDTATGDGIAMAWRAGCRVSNMEFIQFHPTCLY
HPYAKSFLISEAVRGEGGLLKLPDGTRFMPEHDERAELAPRDVVARAIDFEMKKRGLDCVYLDITHQPEAFLKEHFPTIH
ARCLELGIDIAREPIPVVPAAHYTCGGVVTDTLGRTDIGNLYAVGETACTGLHGANRLASNSLLECIVIGRAAATDIASQ
DKAGLPDLALPSWDESRVSDADEEVVVSHNWDELRRMMWNYVGIVRTSKRLERAQHRIRLLREEIAEYYANFRVTRDLLE
LRNLVEVASLIVDSAYSRHESRGLHFSRDYPDTLPKALPTVMQPARKR

Sequences:

>Translated_528_residues
MNFDVAIVGSGLAGLTVALQLADTHRVVILSKRAMTQGASDWAQGGIAAVLDSGDSHDEHTQDTLVAGAGLCDEEATRFI
VEHGREAIQWLIDRGVPFTKDEQAELGYHLTREGGHSRRRIIHAADATGHAVVSTLSEMARQHPNITVIEDQFAIDLITS
RKLGLPGNRCYGLYVLDDSTGEVRTITATHTVLAAGGAGKVYLYTTNPDTATGDGIAMAWRAGCRVSNMEFIQFHPTCLY
HPYAKSFLISEAVRGEGGLLKLPDGTRFMPEHDERAELAPRDVVARAIDFEMKKRGLDCVYLDITHQPEAFLKEHFPTIH
ARCLELGIDIAREPIPVVPAAHYTCGGVVTDTLGRTDIGNLYAVGETACTGLHGANRLASNSLLECIVIGRAAATDIASQ
DKAGLPDLALPSWDESRVSDADEEVVVSHNWDELRRMMWNYVGIVRTSKRLERAQHRIRLLREEIAEYYANFRVTRDLLE
LRNLVEVASLIVDSAYSRHESRGLHFSRDYPDTLPKALPTVMQPARKR
>Mature_528_residues
MNFDVAIVGSGLAGLTVALQLADTHRVVILSKRAMTQGASDWAQGGIAAVLDSGDSHDEHTQDTLVAGAGLCDEEATRFI
VEHGREAIQWLIDRGVPFTKDEQAELGYHLTREGGHSRRRIIHAADATGHAVVSTLSEMARQHPNITVIEDQFAIDLITS
RKLGLPGNRCYGLYVLDDSTGEVRTITATHTVLAAGGAGKVYLYTTNPDTATGDGIAMAWRAGCRVSNMEFIQFHPTCLY
HPYAKSFLISEAVRGEGGLLKLPDGTRFMPEHDERAELAPRDVVARAIDFEMKKRGLDCVYLDITHQPEAFLKEHFPTIH
ARCLELGIDIAREPIPVVPAAHYTCGGVVTDTLGRTDIGNLYAVGETACTGLHGANRLASNSLLECIVIGRAAATDIASQ
DKAGLPDLALPSWDESRVSDADEEVVVSHNWDELRRMMWNYVGIVRTSKRLERAQHRIRLLREEIAEYYANFRVTRDLLE
LRNLVEVASLIVDSAYSRHESRGLHFSRDYPDTLPKALPTVMQPARKR

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=505, Percent_Identity=33.4653465346535, Blast_Score=231, Evalue=9e-61,
Organism=Escherichia coli, GI1788928, Length=525, Percent_Identity=60.3809523809524, Blast_Score=653, Evalue=0.0,
Organism=Escherichia coli, GI1790597, Length=520, Percent_Identity=33.8461538461538, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1786942, Length=558, Percent_Identity=33.6917562724014, Blast_Score=223, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17550100, Length=531, Percent_Identity=32.5800376647834, Blast_Score=234, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI17505833, Length=536, Percent_Identity=32.6492537313433, Blast_Score=231, Evalue=9e-61,
Organism=Caenorhabditis elegans, GI71986328, Length=463, Percent_Identity=28.2937365010799, Blast_Score=103, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6322701, Length=553, Percent_Identity=34.3580470162749, Blast_Score=244, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6322416, Length=550, Percent_Identity=34, Blast_Score=234, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6320788, Length=487, Percent_Identity=28.952772073922, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6322511, Length=484, Percent_Identity=26.8595041322314, Blast_Score=113, Evalue=7e-26,
Organism=Drosophila melanogaster, GI17137288, Length=532, Percent_Identity=32.5187969924812, Blast_Score=236, Evalue=3e-62,
Organism=Drosophila melanogaster, GI24655642, Length=532, Percent_Identity=32.5187969924812, Blast_Score=236, Evalue=3e-62,
Organism=Drosophila melanogaster, GI24655647, Length=532, Percent_Identity=32.5187969924812, Blast_Score=236, Evalue=3e-62,
Organism=Drosophila melanogaster, GI24663005, Length=549, Percent_Identity=32.7868852459016, Blast_Score=229, Evalue=3e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 58176; Mature: 58176

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFDVAIVGSGLAGLTVALQLADTHRVVILSKRAMTQGASDWAQGGIAAVLDSGDSHDEH
CCEEEEEECCCCCCEEEEEEECCCCEEEEEECHHHHCCCCHHHCCCEEEEECCCCCCCCH
TQDTLVAGAGLCDEEATRFIVEHGREAIQWLIDRGVPFTKDEQAELGYHLTREGGHSRRR
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCEEEECCCCCCHHE
IIHAADATGHAVVSTLSEMARQHPNITVIEDQFAIDLITSRKLGLPGNRCYGLYVLDDST
EEEECCCCHHHHHHHHHHHHHHCCCEEEEECCEEEEEEECCCCCCCCCCEEEEEEEECCC
GEVRTITATHTVLAAGGAGKVYLYTTNPDTATGDGIAMAWRAGCRVSNMEFIQFHPTCLY
CCEEEEEEEEEEEEECCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCEEEEEECCCEEE
HPYAKSFLISEAVRGEGGLLKLPDGTRFMPEHDERAELAPRDVVARAIDFEMKKRGLDCV
CHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCEE
YLDITHQPEAFLKEHFPTIHARCLELGIDIAREPIPVVPAAHYTCGGVVTDTLGRTDIGN
EEEECCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCCC
LYAVGETACTGLHGANRLASNSLLECIVIGRAAATDIASQDKAGLPDLALPSWDESRVSD
EEEECHHHHCCCCCHHHHHHHHHEEHEEECCHHHHHHCCCCCCCCCCCCCCCCCCHHCCC
ADEEVVVSHNWDELRRMMWNYVGIVRTSKRLERAQHRIRLLREEIAEYYANFRVTRDLLE
CCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRNLVEVASLIVDSAYSRHESRGLHFSRDYPDTLPKALPTVMQPARKR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNFDVAIVGSGLAGLTVALQLADTHRVVILSKRAMTQGASDWAQGGIAAVLDSGDSHDEH
CCEEEEEECCCCCCEEEEEEECCCCEEEEEECHHHHCCCCHHHCCCEEEEECCCCCCCCH
TQDTLVAGAGLCDEEATRFIVEHGREAIQWLIDRGVPFTKDEQAELGYHLTREGGHSRRR
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCEEEECCCCCCHHE
IIHAADATGHAVVSTLSEMARQHPNITVIEDQFAIDLITSRKLGLPGNRCYGLYVLDDST
EEEECCCCHHHHHHHHHHHHHHCCCEEEEECCEEEEEEECCCCCCCCCCEEEEEEEECCC
GEVRTITATHTVLAAGGAGKVYLYTTNPDTATGDGIAMAWRAGCRVSNMEFIQFHPTCLY
CCEEEEEEEEEEEEECCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCEEEEEECCCEEE
HPYAKSFLISEAVRGEGGLLKLPDGTRFMPEHDERAELAPRDVVARAIDFEMKKRGLDCV
CHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCEE
YLDITHQPEAFLKEHFPTIHARCLELGIDIAREPIPVVPAAHYTCGGVVTDTLGRTDIGN
EEEECCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCCC
LYAVGETACTGLHGANRLASNSLLECIVIGRAAATDIASQDKAGLPDLALPSWDESRVSD
EEEECHHHHCCCCCHHHHHHHHHEEHEEECCHHHHHHCCCCCCCCCCCCCCCCCCHHCCC
ADEEVVVSHNWDELRRMMWNYVGIVRTSKRLERAQHRIRLLREEIAEYYANFRVTRDLLE
CCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRNLVEVASLIVDSAYSRHESRGLHFSRDYPDTLPKALPTVMQPARKR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]