Click here to switch to the map view.

The map label for this gene is cusS [H]

Identifier: 73542176

GI number: 73542176

Start: 2731002

End: 2732399

Strand: Direct

Name: cusS [H]

Synonym: Reut_A2491

Alternate gene names: 73542176

Gene position: 2731002-2732399 (Clockwise)

Preceding gene: 73542175

Following gene: 73542182

Centisome position: 71.75

GC content: 65.74

Gene sequence:

>1398_bases
ATGCGCCGGTTCTCGCTGACCACGCGACTGACCGCGCTGTTCTGCCTGTCGTCGGCCGGCGTGCTGCTGGGCCTCGGCAT
CCTGATCGCGACGGCCATGGACCAGCACTTTGCGGAAGAGGACTTCGCGAGCCTTGGCGACAACGTGCGGCTGATCGAGC
GGATCGCCACCGAGAACGCGGCAGCGTCCGTGTCCGAACGGCTGGCCGTGGCACTCGAGCATCATCCCGGATTCATCGCG
CATGTCAGCGCGGCCGACGGAACGCTACTGTATGCGAGCAACGGTTTTGATTTCCGCACGGTGCTGGGCACCGCTTCGGT
ACTGCCGGCCGGGCGCGAAAGTTTTGACTGGGAACAGGACGGCAAGTCGTATCGAGGCATGCGCGCCACCGTGCAGGCAA
CAGGCGACAAGCAGGTGCCGCTGACCATCGTGGTCGGCATGGACACGGAAATCCACGCGCATTTCATGCGAGCGTTCCGC
CGCTCGCTGACCTTCTACGTCACGCTGGCCGCATTGGCCAGCGGCCTGCTCGGCTGGTGGGCGGCACGTCGCGGGCTGGC
GCCGTTGCGCACCATGGCCAGCCGCGCGCGTGCCGTGACCGCACACAAGCTGGACGCGCGCATGCCTGTGGACGCAGTGC
CGGTAGAAATGGCAGACCTGGCAGGTACGCTGAACGCGATGCTCGAGCGCCTGCAGAGCGATTTCCAACGGCTATCCGAT
TTCTCTTCCGACATCGCGCACGAGTTGCGCACCCCGCTCACCAACCTGATGACGCAGACGCACGTGGTGCTCTCGCAGCC
GCGCGCTGCCGAAAAGTACCGCGACGTGCTGGCGTCCAACGCCGAGGAGCTGCAACGCCTTTCACGCATGGTGTCGGACA
TGCTGTACCTGGCCCAGATGGAACACGGCCTGACACTGCCCAGCGCCGAGCCGATCGACGTCGCGGTCGAGGTCCATGCC
TTGTTCGAATTCTATGATGCGCTGGCCGAAGACAAGGCGGTGCGCCTGCAATTGCGCGGCGAAGGGCATATCGTCGGCGA
CCGCCTGATGCTGCGCCGCGCGCTCAGCAACCTGCTGTCCAACGCGCTGCGCTATACGCCGCCCGGTGGCGTTATCGTGA
TCGAGATCGGCGGCAGCGCGGATACCATTACGGTCTCGGTAGAGAATGATGGTCAGGACATCCGCCCGGATTTGCTGCCC
GTGATCTTCGACCGCTTCTCGCGCGGCGATAAATCGCGGGTGCGGCCGGAGTCGGACAGCGTGGGGCTTGGGCTGTCGAT
CACGCGCGCGATCATGGCCGCACATGGCGGCACCATCGCCGTGCAGTCCGCGAGCGGCAAGACGCGCTTTACGCTCGCGT
TCCGACGACAGGCGGCGCCTGCTGCAGCGACGGCCTGA

Upstream 100 bases:

>100_bases
GCCGCCTGCGCGCGAAGATCGACGACGACTTCGACACCAAGCTGATCCAGACGGTGCGCGGCATGGGCTACGTGCTGGAA
GGTCCCGAGGACACGGTGTG

Downstream 100 bases:

>100_bases
GACGCGTCATTCGCTGCCGGTCAGCAGGCGATACGCCTCGTCTTCGGTAATCCTCAAGCGGTATTTGGTGGACAGCATCC
GGGCCATCGAATGCGCGGTG

Product: heavy metal sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MRRFSLTTRLTALFCLSSAGVLLGLGILIATAMDQHFAEEDFASLGDNVRLIERIATENAAASVSERLAVALEHHPGFIA
HVSAADGTLLYASNGFDFRTVLGTASVLPAGRESFDWEQDGKSYRGMRATVQATGDKQVPLTIVVGMDTEIHAHFMRAFR
RSLTFYVTLAALASGLLGWWAARRGLAPLRTMASRARAVTAHKLDARMPVDAVPVEMADLAGTLNAMLERLQSDFQRLSD
FSSDIAHELRTPLTNLMTQTHVVLSQPRAAEKYRDVLASNAEELQRLSRMVSDMLYLAQMEHGLTLPSAEPIDVAVEVHA
LFEFYDALAEDKAVRLQLRGEGHIVGDRLMLRRALSNLLSNALRYTPPGGVIVIEIGGSADTITVSVENDGQDIRPDLLP
VIFDRFSRGDKSRVRPESDSVGLGLSITRAIMAAHGGTIAVQSASGKTRFTLAFRRQAAPAAATA

Sequences:

>Translated_465_residues
MRRFSLTTRLTALFCLSSAGVLLGLGILIATAMDQHFAEEDFASLGDNVRLIERIATENAAASVSERLAVALEHHPGFIA
HVSAADGTLLYASNGFDFRTVLGTASVLPAGRESFDWEQDGKSYRGMRATVQATGDKQVPLTIVVGMDTEIHAHFMRAFR
RSLTFYVTLAALASGLLGWWAARRGLAPLRTMASRARAVTAHKLDARMPVDAVPVEMADLAGTLNAMLERLQSDFQRLSD
FSSDIAHELRTPLTNLMTQTHVVLSQPRAAEKYRDVLASNAEELQRLSRMVSDMLYLAQMEHGLTLPSAEPIDVAVEVHA
LFEFYDALAEDKAVRLQLRGEGHIVGDRLMLRRALSNLLSNALRYTPPGGVIVIEIGGSADTITVSVENDGQDIRPDLLP
VIFDRFSRGDKSRVRPESDSVGLGLSITRAIMAAHGGTIAVQSASGKTRFTLAFRRQAAPAAATA
>Mature_465_residues
MRRFSLTTRLTALFCLSSAGVLLGLGILIATAMDQHFAEEDFASLGDNVRLIERIATENAAASVSERLAVALEHHPGFIA
HVSAADGTLLYASNGFDFRTVLGTASVLPAGRESFDWEQDGKSYRGMRATVQATGDKQVPLTIVVGMDTEIHAHFMRAFR
RSLTFYVTLAALASGLLGWWAARRGLAPLRTMASRARAVTAHKLDARMPVDAVPVEMADLAGTLNAMLERLQSDFQRLSD
FSSDIAHELRTPLTNLMTQTHVVLSQPRAAEKYRDVLASNAEELQRLSRMVSDMLYLAQMEHGLTLPSAEPIDVAVEVHA
LFEFYDALAEDKAVRLQLRGEGHIVGDRLMLRRALSNLLSNALRYTPPGGVIVIEIGGSADTITVSVENDGQDIRPDLLP
VIFDRFSRGDKSRVRPESDSVGLGLSITRAIMAAHGGTIAVQSASGKTRFTLAFRRQAAPAAATA

Specific function: Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Probably activates CusR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=476, Percent_Identity=35.0840336134454, Blast_Score=274, Evalue=8e-75,
Organism=Escherichia coli, GI1788279, Length=304, Percent_Identity=32.5657894736842, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI1786600, Length=216, Percent_Identity=30.0925925925926, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788393, Length=270, Percent_Identity=28.8888888888889, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1790346, Length=269, Percent_Identity=32.3420074349442, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1787894, Length=222, Percent_Identity=31.981981981982, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1787374, Length=387, Percent_Identity=27.390180878553, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790551, Length=309, Percent_Identity=27.5080906148867, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI48994928, Length=230, Percent_Identity=29.1304347826087, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1786912, Length=222, Percent_Identity=29.7297297297297, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1789403, Length=469, Percent_Identity=26.0127931769723, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790436, Length=232, Percent_Identity=27.1551724137931, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789149, Length=318, Percent_Identity=23.5849056603774, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1788713, Length=235, Percent_Identity=24.2553191489362, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50493; Mature: 50493

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRFSLTTRLTALFCLSSAGVLLGLGILIATAMDQHFAEEDFASLGDNVRLIERIATENA
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AASVSERLAVALEHHPGFIAHVSAADGTLLYASNGFDFRTVLGTASVLPAGRESFDWEQD
HHHHHHHHHHHEECCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCHHC
GKSYRGMRATVQATGDKQVPLTIVVGMDTEIHAHFMRAFRRSLTFYVTLAALASGLLGWW
CCHHCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AARRGLAPLRTMASRARAVTAHKLDARMPVDAVPVEMADLAGTLNAMLERLQSDFQRLSD
HHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FSSDIAHELRTPLTNLMTQTHVVLSQPRAAEKYRDVLASNAEELQRLSRMVSDMLYLAQM
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
EHGLTLPSAEPIDVAVEVHALFEFYDALAEDKAVRLQLRGEGHIVGDRLMLRRALSNLLS
HCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEHHHHHHHHHHHHHHH
NALRYTPPGGVIVIEIGGSADTITVSVENDGQDIRPDLLPVIFDRFSRGDKSRVRPESDS
HHHCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
VGLGLSITRAIMAAHGGTIAVQSASGKTRFTLAFRRQAAPAAATA
CCCCHHHHHHHHHHCCCEEEEECCCCCEEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MRRFSLTTRLTALFCLSSAGVLLGLGILIATAMDQHFAEEDFASLGDNVRLIERIATENA
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AASVSERLAVALEHHPGFIAHVSAADGTLLYASNGFDFRTVLGTASVLPAGRESFDWEQD
HHHHHHHHHHHEECCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCHHC
GKSYRGMRATVQATGDKQVPLTIVVGMDTEIHAHFMRAFRRSLTFYVTLAALASGLLGWW
CCHHCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AARRGLAPLRTMASRARAVTAHKLDARMPVDAVPVEMADLAGTLNAMLERLQSDFQRLSD
HHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FSSDIAHELRTPLTNLMTQTHVVLSQPRAAEKYRDVLASNAEELQRLSRMVSDMLYLAQM
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
EHGLTLPSAEPIDVAVEVHALFEFYDALAEDKAVRLQLRGEGHIVGDRLMLRRALSNLLS
HCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEHHHHHHHHHHHHHHH
NALRYTPPGGVIVIEIGGSADTITVSVENDGQDIRPDLLPVIFDRFSRGDKSRVRPESDS
HHHCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
VGLGLSITRAIMAAHGGTIAVQSASGKTRFTLAFRRQAAPAAATA
CCCCHHHHHHHHHHCCCEEEEECCCCCEEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]