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The map label for this gene is ntrB [H]

Identifier: 73541743

GI number: 73541743

Start: 2258101

End: 2259282

Strand: Reverse

Name: ntrB [H]

Synonym: Reut_A2055

Alternate gene names: 73541743

Gene position: 2259282-2258101 (Counterclockwise)

Preceding gene: 73541744

Following gene: 73541742

Centisome position: 59.35

GC content: 63.62

Gene sequence:

>1182_bases
ATGCGTCGCCTGATTCGCAGCGTGTCGCGCAGGAGCGGTAGCGCCGATGGCGCCGGCGGCGACACTGCCGGCCGGACTCC
ACCGGCCAATGGTCCGGGCAGCGGTCAGGTGTTGCCACTAGGCGCGGTGGCGCTGCATGCCGGCCTCGATGTCGTCGCTA
ACCCGGTACTGCTCGTCAGGCAACCGGGTCTGGGCGTGATGTATGCCAATCCGGCGGCGGAATCGACCTTCGCAGTGTCG
CGCAAGGCGATGGTGGAGTTGTCGTTGTCGGACCTGTTCGGGCAGTCCGAAGAGCTGCAGGGCATGATCGACACGGTGAT
CACGCGCCAGTTCGATGTGCGGCGCCAGGACTTGGTGCTGCGCCCACCATTGCAGGAGCCCATTCACGCGCACGTGGTCA
TCGGCGCGCTGGAGGCCTTCAGCGATACCGTGCTCGTTGAGATCCTGCCCAACGAGCAGAAGGTTCGCAGCGACAGGGAA
GAGCGCATTCTCGACCTGACGGCCGCTAACAAGGAATTGATCCGCAATCTCGCCCACGAGATCAAGAATCCGCTCGGCGG
TATCCGTGGCGCGGCACAACTGCTCGAGTTCGAGTTGCCCGAACGTGCACTGCGCGAATACACGCAGGTCATCATCAAGG
AGTCGGACCGGTTGCAGACGCTGGTGGACCGGCTGCTGGAGCCGCACCGCCATCCGCATATCGTGTCGAGCCTGAATATC
CACGAGGTGCTAGAGCGTGTGCGTTCGGTCGTGCTGGCCGAGTTTCCCAACGGGCTGGACATCGAGCGCGATTACGATGC
CAGCCTGCCCGAACTGCGCGGCGACATGGAACAGCTGATCCAGGCCGTGCTCAATATCGTCCATAACGCAGCCCAGGCGC
TGGCCGAGCGCATTGCGCGCGGCGACGCGCAGATCATCCTGCGCACGCGCGTCGCCCGCCAGGTCACGATTGCGAAGCGA
TTGTTCAAGCTGGCATTGGACTTGCATGTGATCGACAACGGCCCGGGAATTCCCGATGACATCCGCGAACGCATCTTCTA
TCCGCTGGTTTCGGGTAGGGACGGCGGCAGCGGACTCGGTCTCACACTCGCTCAAACCTTCGTGCAGCAGCACGAGGGCT
TGATCGAATGCGAGAGCAGGCCGGGCTGTACCGACTTCCGCATCCTGCTGCCGCTGCACTAG

Upstream 100 bases:

>100_bases
TATCTGGATCGCACGGCAGCCATGCGCGATGACATCGCCCGCAGCGAAGCCAACGCGGCGTCGTTGCGCCGCGAGCTTTC
CAACTTGAAGGACTAGTCAT

Downstream 100 bases:

>100_bases
CTGTCCGCCGGCATCCGCGATCGATCGTTGCACTTGCCGCCAGGCCGGGCATGCGGAAGACCAAATGACACCGAGCAGAC
AAGCGACGCACATGAAGCCG

Product: signal transduction histidine kinase, nitrogen specific, NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MRRLIRSVSRRSGSADGAGGDTAGRTPPANGPGSGQVLPLGAVALHAGLDVVANPVLLVRQPGLGVMYANPAAESTFAVS
RKAMVELSLSDLFGQSEELQGMIDTVITRQFDVRRQDLVLRPPLQEPIHAHVVIGALEAFSDTVLVEILPNEQKVRSDRE
ERILDLTAANKELIRNLAHEIKNPLGGIRGAAQLLEFELPERALREYTQVIIKESDRLQTLVDRLLEPHRHPHIVSSLNI
HEVLERVRSVVLAEFPNGLDIERDYDASLPELRGDMEQLIQAVLNIVHNAAQALAERIARGDAQIILRTRVARQVTIAKR
LFKLALDLHVIDNGPGIPDDIRERIFYPLVSGRDGGSGLGLTLAQTFVQQHEGLIECESRPGCTDFRILLPLH

Sequences:

>Translated_393_residues
MRRLIRSVSRRSGSADGAGGDTAGRTPPANGPGSGQVLPLGAVALHAGLDVVANPVLLVRQPGLGVMYANPAAESTFAVS
RKAMVELSLSDLFGQSEELQGMIDTVITRQFDVRRQDLVLRPPLQEPIHAHVVIGALEAFSDTVLVEILPNEQKVRSDRE
ERILDLTAANKELIRNLAHEIKNPLGGIRGAAQLLEFELPERALREYTQVIIKESDRLQTLVDRLLEPHRHPHIVSSLNI
HEVLERVRSVVLAEFPNGLDIERDYDASLPELRGDMEQLIQAVLNIVHNAAQALAERIARGDAQIILRTRVARQVTIAKR
LFKLALDLHVIDNGPGIPDDIRERIFYPLVSGRDGGSGLGLTLAQTFVQQHEGLIECESRPGCTDFRILLPLH
>Mature_393_residues
MRRLIRSVSRRSGSADGAGGDTAGRTPPANGPGSGQVLPLGAVALHAGLDVVANPVLLVRQPGLGVMYANPAAESTFAVS
RKAMVELSLSDLFGQSEELQGMIDTVITRQFDVRRQDLVLRPPLQEPIHAHVVIGALEAFSDTVLVEILPNEQKVRSDRE
ERILDLTAANKELIRNLAHEIKNPLGGIRGAAQLLEFELPERALREYTQVIIKESDRLQTLVDRLLEPHRHPHIVSSLNI
HEVLERVRSVVLAEFPNGLDIERDYDASLPELRGDMEQLIQAVLNIVHNAAQALAERIARGDAQIILRTRVARQVTIAKR
LFKLALDLHVIDNGPGIPDDIRERIFYPLVSGRDGGSGLGLTLAQTFVQQHEGLIECESRPGCTDFRILLPLH

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=356, Percent_Identity=41.2921348314607, Blast_Score=236, Evalue=1e-63,
Organism=Escherichia coli, GI1788549, Length=343, Percent_Identity=30.3206997084548, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1790436, Length=252, Percent_Identity=27.7777777777778, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1790346, Length=240, Percent_Identity=25.4166666666667, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 43240; Mature: 43240

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLIRSVSRRSGSADGAGGDTAGRTPPANGPGSGQVLPLGAVALHAGLDVVANPVLLVR
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCHHHHHCCEEEEE
QPGLGVMYANPAAESTFAVSRKAMVELSLSDLFGQSEELQGMIDTVITRQFDVRRQDLVL
CCCCEEEEECCCCCHHHHHHHHHHHEEEHHHHCCCCHHHHHHHHHHHHHHHHCHHHCEEE
RPPLQEPIHAHVVIGALEAFSDTVLVEILPNEQKVRSDREERILDLTAANKELIRNLAHE
CCCCCCCHHHHHHHHHHHHHCCEEEEEECCCHHHHHCCHHHHHHHHHHCCHHHHHHHHHH
IKNPLGGIRGAAQLLEFELPERALREYTQVIIKESDRLQTLVDRLLEPHRHPHIVSSLNI
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHCCCH
HEVLERVRSVVLAEFPNGLDIERDYDASLPELRGDMEQLIQAVLNIVHNAAQALAERIAR
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GDAQIILRTRVARQVTIAKRLFKLALDLHVIDNGPGIPDDIRERIFYPLVSGRDGGSGLG
CCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCH
LTLAQTFVQQHEGLIECESRPGCTDFRILLPLH
HHHHHHHHHHCCCCEEECCCCCCCCEEEEEECC
>Mature Secondary Structure
MRRLIRSVSRRSGSADGAGGDTAGRTPPANGPGSGQVLPLGAVALHAGLDVVANPVLLVR
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCHHHHHCCEEEEE
QPGLGVMYANPAAESTFAVSRKAMVELSLSDLFGQSEELQGMIDTVITRQFDVRRQDLVL
CCCCEEEEECCCCCHHHHHHHHHHHEEEHHHHCCCCHHHHHHHHHHHHHHHHCHHHCEEE
RPPLQEPIHAHVVIGALEAFSDTVLVEILPNEQKVRSDREERILDLTAANKELIRNLAHE
CCCCCCCHHHHHHHHHHHHHCCEEEEEECCCHHHHHCCHHHHHHHHHHCCHHHHHHHHHH
IKNPLGGIRGAAQLLEFELPERALREYTQVIIKESDRLQTLVDRLLEPHRHPHIVSSLNI
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHCCCH
HEVLERVRSVVLAEFPNGLDIERDYDASLPELRGDMEQLIQAVLNIVHNAAQALAERIAR
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GDAQIILRTRVARQVTIAKRLFKLALDLHVIDNGPGIPDDIRERIFYPLVSGRDGGSGLG
CCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCH
LTLAQTFVQQHEGLIECESRPGCTDFRILLPLH
HHHHHHHHHHCCCCEEECCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2574025 [H]