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The map label for this gene is glnG [H]

Identifier: 73541742

GI number: 73541742

Start: 2256462

End: 2258036

Strand: Reverse

Name: glnG [H]

Synonym: Reut_A2054

Alternate gene names: 73541742

Gene position: 2258036-2256462 (Counterclockwise)

Preceding gene: 73541743

Following gene: 73541740

Centisome position: 59.32

GC content: 64.95

Gene sequence:

>1575_bases
ATGACACCGAGCAGACAAGCGACGCACATGAAGCCGATCTGGATAGTCGACGACGATCAATCAATCCGCTGGGTACTTGA
AAAGGCCCTGGCCCGTGAAAGCCTGCTCTCGCGCAGCTTTACCAATGTCCGCGACGCACTCGCGGCGCTGGAAGATGATG
TTCCGCAGGTCCTGATATCGGATATCCGCATGCCCGGCGGCTCGGGCCTGGACCTGCTGCAGGCCATCAAGGCGAAGCAC
CCAGGCCTGCCGGTCATCATCATGACGGCCTATTCGGACCTGGACAGCGCCGTGGCGGCATTCCAGGGCGGCGCCTTCGA
ATATCTGGCCAAGCCGTTCGATGTCGACAAGGCCGTCGAACTGATCCGCCGCGCGCTCGAAGAAAGCCTGCGCGAGGAGG
AGATGGACGACCGGCTCGTCGATGCGCCGGAAATCCTCGGGCAGGCGCCCGCGATGCAGGACGTGTTCCGCGCGATCGGC
CGCCTGTCGCAATCGAATGTGACGGTGATGATTACGGGCGAGTCCGGTACGGGCAAGGAACTCGTCGCGCGAGCCTTGCA
CAAGCACAGCCCGCGCGCGAATGGCCCGTTCATTGCGCTGAACACGGCCGCCATCCCGAAGGATCTGCTCGAATCCGAAC
TGTTCGGCCATGAGCGCGGCGCGTTCACGGGCGCGCAAACCATGCGCCGCGGTCGCTTCGAGCAGGCCGAGGGCGGCACG
CTGTTCCTCGATGAAATCGGCGATATGCCGTTCGACCTGCAGACCCGGCTGCTGCGTGTGCTGTCCGACGGAAACTTCTA
CCGTGTGGGCGGGCACAATCCGCTGCGCGCCAATGTTCGCGTGATTGCCGCCACGCACCAGAACCTGGAGTTGCGCGTCA
AGGAGGGCCTGTTCCGCGAAGACTTGTTCCACCGCCTGAACGTGATCCGGCTACGCCTGCCGCCGCTGCGCGAACGTCCC
GAGGACATCACGCTGCTGGCACGGCATTTCCTGCAGAAGAGCGCGAAGGAACTGGGCGTGGAGCCCAAGCGCATGTCCGA
CGAGGCGCTGGCGTACGTGAGCACGCTGCCGTTCCCGGGCAACGTGCGCCAGCTAGAGAACCTCTGCAACTGGCTTACCG
TGATGGCGCCGGCCCAGACCATCGAGGTCAAGGACTTGCCGCGCGAGATGATCGGCACCGGCAGTGCGGAAAGCGCGCCG
GTGCCGCGTGCGGTCGAGGCGCGTTTGCCATCGGCTGATTTCGAGGGCGGCCATGATCTTTCCGATTACGGCGGACTGGC
CATGCCAGTCGCCGAGGCGGACACCATGACCGCGGTGAGGCCAGTCACGGCGACCTCGATGCCAGCACCTTCGTCGCTCG
CGGCCGGATGGGAAACCCTGCTCGCTGGCGAGGCAAAGGCAATGCTGGAGTCCGGCCAGCCGGAAGTCATGGACGTGCTG
ACGCGGCGCTTCGAAAAGGCCGTACTGGAGGCGGCGCTCGGTGTGACGCGGGGCCGCCGCGTGGAAGCTGCCACGCGGCT
TGGCATCGGACGCAATACAATCACGCGCAAGCTGCAGGAACTGGGCTTCGACTAA

Upstream 100 bases:

>100_bases
TGTACCGACTTCCGCATCCTGCTGCCGCTGCACTAGCTGTCCGCCGGCATCCGCGATCGATCGTTGCACTTGCCGCCAGG
CCGGGCATGCGGAAGACCAA

Downstream 100 bases:

>100_bases
GCTACCGATGATGGCTTTCAGCGAAAAGGCCGGGTTCGCCCCGGCCTTTTCTCTTGCTATGCACTATCCGAGCGCGCCGC
AAGCAAACGCTCGGCCATCT

Product: helix-turn-helix, Fis-type:nitrogen regulation protein NR(I)

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MTPSRQATHMKPIWIVDDDQSIRWVLEKALARESLLSRSFTNVRDALAALEDDVPQVLISDIRMPGGSGLDLLQAIKAKH
PGLPVIIMTAYSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRALEESLREEEMDDRLVDAPEILGQAPAMQDVFRAIG
RLSQSNVTVMITGESGTGKELVARALHKHSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQTMRRGRFEQAEGGT
LFLDEIGDMPFDLQTRLLRVLSDGNFYRVGGHNPLRANVRVIAATHQNLELRVKEGLFREDLFHRLNVIRLRLPPLRERP
EDITLLARHFLQKSAKELGVEPKRMSDEALAYVSTLPFPGNVRQLENLCNWLTVMAPAQTIEVKDLPREMIGTGSAESAP
VPRAVEARLPSADFEGGHDLSDYGGLAMPVAEADTMTAVRPVTATSMPAPSSLAAGWETLLAGEAKAMLESGQPEVMDVL
TRRFEKAVLEAALGVTRGRRVEAATRLGIGRNTITRKLQELGFD

Sequences:

>Translated_524_residues
MTPSRQATHMKPIWIVDDDQSIRWVLEKALARESLLSRSFTNVRDALAALEDDVPQVLISDIRMPGGSGLDLLQAIKAKH
PGLPVIIMTAYSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRALEESLREEEMDDRLVDAPEILGQAPAMQDVFRAIG
RLSQSNVTVMITGESGTGKELVARALHKHSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQTMRRGRFEQAEGGT
LFLDEIGDMPFDLQTRLLRVLSDGNFYRVGGHNPLRANVRVIAATHQNLELRVKEGLFREDLFHRLNVIRLRLPPLRERP
EDITLLARHFLQKSAKELGVEPKRMSDEALAYVSTLPFPGNVRQLENLCNWLTVMAPAQTIEVKDLPREMIGTGSAESAP
VPRAVEARLPSADFEGGHDLSDYGGLAMPVAEADTMTAVRPVTATSMPAPSSLAAGWETLLAGEAKAMLESGQPEVMDVL
TRRFEKAVLEAALGVTRGRRVEAATRLGIGRNTITRKLQELGFD
>Mature_523_residues
TPSRQATHMKPIWIVDDDQSIRWVLEKALARESLLSRSFTNVRDALAALEDDVPQVLISDIRMPGGSGLDLLQAIKAKHP
GLPVIIMTAYSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRALEESLREEEMDDRLVDAPEILGQAPAMQDVFRAIGR
LSQSNVTVMITGESGTGKELVARALHKHSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQTMRRGRFEQAEGGTL
FLDEIGDMPFDLQTRLLRVLSDGNFYRVGGHNPLRANVRVIAATHQNLELRVKEGLFREDLFHRLNVIRLRLPPLRERPE
DITLLARHFLQKSAKELGVEPKRMSDEALAYVSTLPFPGNVRQLENLCNWLTVMAPAQTIEVKDLPREMIGTGSAESAPV
PRAVEARLPSADFEGGHDLSDYGGLAMPVAEADTMTAVRPVTATSMPAPSSLAAGWETLLAGEAKAMLESGQPEVMDVLT
RRFEKAVLEAALGVTRGRRVEAATRLGIGRNTITRKLQELGFD

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=514, Percent_Identity=56.2256809338521, Blast_Score=554, Evalue=1e-159,
Organism=Escherichia coli, GI1788550, Length=404, Percent_Identity=45.2970297029703, Blast_Score=351, Evalue=8e-98,
Organism=Escherichia coli, GI1790437, Length=393, Percent_Identity=42.2391857506361, Blast_Score=291, Evalue=5e-80,
Organism=Escherichia coli, GI1788905, Length=396, Percent_Identity=41.9191919191919, Blast_Score=281, Evalue=6e-77,
Organism=Escherichia coli, GI87082117, Length=248, Percent_Identity=51.2096774193548, Blast_Score=249, Evalue=4e-67,
Organism=Escherichia coli, GI1789233, Length=287, Percent_Identity=45.6445993031359, Blast_Score=236, Evalue=4e-63,
Organism=Escherichia coli, GI1789087, Length=249, Percent_Identity=48.995983935743, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI87082152, Length=239, Percent_Identity=47.6987447698745, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI1786524, Length=245, Percent_Identity=48.1632653061224, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1787583, Length=224, Percent_Identity=45.5357142857143, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI87081872, Length=229, Percent_Identity=46.7248908296943, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1789828, Length=211, Percent_Identity=36.0189573459716, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI87081858, Length=241, Percent_Identity=31.5352697095436, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1790860, Length=114, Percent_Identity=32.4561403508772, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 57543; Mature: 57412

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50330 UIM ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPSRQATHMKPIWIVDDDQSIRWVLEKALARESLLSRSFTNVRDALAALEDDVPQVLIS
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
DIRMPGGSGLDLLQAIKAKHPGLPVIIMTAYSDLDSAVAAFQGGAFEYLAKPFDVDKAVE
HHCCCCCCCHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHCCCCHHHHCCCCCHHHHHH
LIRRALEESLREEEMDDRLVDAPEILGQAPAMQDVFRAIGRLSQSNVTVMITGESGTGKE
HHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCHH
LVARALHKHSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQTMRRGRFEQAEGGT
HHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCE
LFLDEIGDMPFDLQTRLLRVLSDGNFYRVGGHNPLRANVRVIAATHQNLELRVKEGLFRE
EEHHHHCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEECCCCEEHHHHCHHHH
DLFHRLNVIRLRLPPLRERPEDITLLARHFLQKSAKELGVEPKRMSDEALAYVSTLPFPG
HHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCC
NVRQLENLCNWLTVMAPAQTIEVKDLPREMIGTGSAESAPVPRAVEARLPSADFEGGHDL
CHHHHHHHHHHHHHHCCCCEEEHHHCCHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCC
SDYGGLAMPVAEADTMTAVRPVTATSMPAPSSLAAGWETLLAGEAKAMLESGQPEVMDVL
HHCCCCEEECCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
TRRFEKAVLEAALGVTRGRRVEAATRLGIGRNTITRKLQELGFD
HHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TPSRQATHMKPIWIVDDDQSIRWVLEKALARESLLSRSFTNVRDALAALEDDVPQVLIS
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
DIRMPGGSGLDLLQAIKAKHPGLPVIIMTAYSDLDSAVAAFQGGAFEYLAKPFDVDKAVE
HHCCCCCCCHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHCCCCHHHHCCCCCHHHHHH
LIRRALEESLREEEMDDRLVDAPEILGQAPAMQDVFRAIGRLSQSNVTVMITGESGTGKE
HHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCHH
LVARALHKHSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQTMRRGRFEQAEGGT
HHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCE
LFLDEIGDMPFDLQTRLLRVLSDGNFYRVGGHNPLRANVRVIAATHQNLELRVKEGLFRE
EEHHHHCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEECCCCEEHHHHCHHHH
DLFHRLNVIRLRLPPLRERPEDITLLARHFLQKSAKELGVEPKRMSDEALAYVSTLPFPG
HHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCC
NVRQLENLCNWLTVMAPAQTIEVKDLPREMIGTGSAESAPVPRAVEARLPSADFEGGHDL
CHHHHHHHHHHHHHHCCCCEEEHHHCCHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCC
SDYGGLAMPVAEADTMTAVRPVTATSMPAPSSLAAGWETLLAGEAKAMLESGQPEVMDVL
HHCCCCEEECCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
TRRFEKAVLEAALGVTRGRRVEAATRLGIGRNTITRKLQELGFD
HHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]