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The map label for this gene is pit [H]

Identifier: 73541688

GI number: 73541688

Start: 2190978

End: 2191985

Strand: Reverse

Name: pit [H]

Synonym: Reut_A2000

Alternate gene names: 73541688

Gene position: 2191985-2190978 (Counterclockwise)

Preceding gene: 73541689

Following gene: 73541682

Centisome position: 57.58

GC content: 63.59

Gene sequence:

>1008_bases
ATGCATACCATTCAAATGAGCCTGTGGGTGATCGGCCTGCTGGTGGCGCTCGCACTGCTGTTCGATTTTATGAACGGCTT
TCACGATGCAGCCAACTCGATCGCCACGGTGGTGTCTACGGGCGTGCTCAAGCCGCACCATGCGGTGGCCATGGCGGCAA
TGTGCAACGTCGTCGCGATCTTCATCTTCCACCTGAAGGTGGCAGCCACCGTCGGTACCGGCACCGTAGACGTCAATATC
GTTGACCACTACGTGATTTTCGGTGCGCTGGTCGGGGCCATCGCGTGGAACGCGATCACGTGGTACTACGGTATTCCTTC
GTCGTCGTCGCATGCGCTGATCGGCGGTTTGGTCGGGGCTGCGGTGGCCAAGGCTGGCACCGGTGCACTGGTGGGCAACG
GCCTGCTGAAGACCGTTGCCTTTATCGTGATCTCGCCGCTGCTGGGTCTCATCCTCGGCTCGCTGATGATGGTGATCGTG
GGCTGGACCTTCTTCCGTACGCCGCCGTCGCGCGTGGACCGCTGGTTCCGCCGGCTGCAGCTTGCGTCGGCGTCGCTGTA
CAGCCTGGGCCACGGCGGCAATGACGCGCAGAAGACCATCGGTATCATCTGGATGCTGCTGATCGCCAGCGGCCATGTCG
CGGCGGGCGGCGAACCGCCGGTGTGGGTGATCGTGAGCTGCTACGTGGCGATTGGCATGGGCACGCTGTTCGGCGGCTGG
CGCATCGTGCGGACGATGGGGCAGAAGATCACCAAGCTGAAGCCTGTCGGCGGCTTCTGTGCCGAAACCGGTGGCGCGAT
GACGCTGTTCCTGGCATCGGCGCTGGGTGTGCCTGTGTCGACTACCCACACGATTACCGGCGCGATCGTCGGTGTGGGCT
CCGCGCAGAAGATGTCGGCGGTCCGCTGGGGCGTGGCTGGCAACATCGTATGGGCCTGGGTGCTGACCATTCCGGCCTCC
GCGTTCATGTCCGCGATAGCGTGGTGGGTTGGCCGCCACATCCTGTAA

Upstream 100 bases:

>100_bases
GCCATCTACGAACAGCTCGAAACGATTACCGACAAGTGCGAGGACGTGGCCAACATCATTGAAGGCATCGTCCTGGAAAA
CGCCTGAAGCGGAATCGGCA

Downstream 100 bases:

>100_bases
GGCTGGGTGGATATCCGCGTATGAAGAAAAGCCAGGCACTTGCCTGGCTTTTTTGTCGTTCTGGAAGTCGCTATTTATTG
GCGAAAGCCGCGATCGGATC

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MHTIQMSLWVIGLLVALALLFDFMNGFHDAANSIATVVSTGVLKPHHAVAMAAMCNVVAIFIFHLKVAATVGTGTVDVNI
VDHYVIFGALVGAIAWNAITWYYGIPSSSSHALIGGLVGAAVAKAGTGALVGNGLLKTVAFIVISPLLGLILGSLMMVIV
GWTFFRTPPSRVDRWFRRLQLASASLYSLGHGGNDAQKTIGIIWMLLIASGHVAAGGEPPVWVIVSCYVAIGMGTLFGGW
RIVRTMGQKITKLKPVGGFCAETGGAMTLFLASALGVPVSTTHTITGAIVGVGSAQKMSAVRWGVAGNIVWAWVLTIPAS
AFMSAIAWWVGRHIL

Sequences:

>Translated_335_residues
MHTIQMSLWVIGLLVALALLFDFMNGFHDAANSIATVVSTGVLKPHHAVAMAAMCNVVAIFIFHLKVAATVGTGTVDVNI
VDHYVIFGALVGAIAWNAITWYYGIPSSSSHALIGGLVGAAVAKAGTGALVGNGLLKTVAFIVISPLLGLILGSLMMVIV
GWTFFRTPPSRVDRWFRRLQLASASLYSLGHGGNDAQKTIGIIWMLLIASGHVAAGGEPPVWVIVSCYVAIGMGTLFGGW
RIVRTMGQKITKLKPVGGFCAETGGAMTLFLASALGVPVSTTHTITGAIVGVGSAQKMSAVRWGVAGNIVWAWVLTIPAS
AFMSAIAWWVGRHIL
>Mature_335_residues
MHTIQMSLWVIGLLVALALLFDFMNGFHDAANSIATVVSTGVLKPHHAVAMAAMCNVVAIFIFHLKVAATVGTGTVDVNI
VDHYVIFGALVGAIAWNAITWYYGIPSSSSHALIGGLVGAAVAKAGTGALVGNGLLKTVAFIVISPLLGLILGSLMMVIV
GWTFFRTPPSRVDRWFRRLQLASASLYSLGHGGNDAQKTIGIIWMLLIASGHVAAGGEPPVWVIVSCYVAIGMGTLFGGW
RIVRTMGQKITKLKPVGGFCAETGGAMTLFLASALGVPVSTTHTITGAIVGVGSAQKMSAVRWGVAGNIVWAWVLTIPAS
AFMSAIAWWVGRHIL

Specific function: Low-affinity inorganic phosphate transport (Probable) [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=173, Percent_Identity=33.5260115606936, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI5803173, Length=153, Percent_Identity=37.9084967320261, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1789907, Length=218, Percent_Identity=33.9449541284404, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789360, Length=217, Percent_Identity=31.7972350230415, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17539280, Length=167, Percent_Identity=34.1317365269461, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI25146401, Length=162, Percent_Identity=30.8641975308642, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71981576, Length=162, Percent_Identity=30.2469135802469, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI32566716, Length=168, Percent_Identity=32.7380952380952, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17536725, Length=185, Percent_Identity=29.1891891891892, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17557328, Length=162, Percent_Identity=32.0987654320988, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319773, Length=164, Percent_Identity=31.7073170731707, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356511, Length=187, Percent_Identity=31.5508021390374, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 35089; Mature: 35089

Theoretical pI: Translated: 10.41; Mature: 10.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTIQMSLWVIGLLVALALLFDFMNGFHDAANSIATVVSTGVLKPHHAVAMAAMCNVVAI
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
FIFHLKVAATVGTGTVDVNIVDHYVIFGALVGAIAWNAITWYYGIPSSSSHALIGGLVGA
HHHHHHHHHEECCCEEEEHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHH
AVAKAGTGALVGNGLLKTVAFIVISPLLGLILGSLMMVIVGWTFFRTPPSRVDRWFRRLQ
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LASASLYSLGHGGNDAQKTIGIIWMLLIASGHVAAGGEPPVWVIVSCYVAIGMGTLFGGW
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RIVRTMGQKITKLKPVGGFCAETGGAMTLFLASALGVPVSTTHTITGAIVGVGSAQKMSA
HHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHEEECCCHHHHHH
VRWGVAGNIVWAWVLTIPASAFMSAIAWWVGRHIL
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHTIQMSLWVIGLLVALALLFDFMNGFHDAANSIATVVSTGVLKPHHAVAMAAMCNVVAI
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
FIFHLKVAATVGTGTVDVNIVDHYVIFGALVGAIAWNAITWYYGIPSSSSHALIGGLVGA
HHHHHHHHHEECCCEEEEHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHH
AVAKAGTGALVGNGLLKTVAFIVISPLLGLILGSLMMVIVGWTFFRTPPSRVDRWFRRLQ
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LASASLYSLGHGGNDAQKTIGIIWMLLIASGHVAAGGEPPVWVIVSCYVAIGMGTLFGGW
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RIVRTMGQKITKLKPVGGFCAETGGAMTLFLASALGVPVSTTHTITGAIVGVGSAQKMSA
HHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHEEECCCHHHHHH
VRWGVAGNIVWAWVLTIPASAFMSAIAWWVGRHIL
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9696772; 11481430 [H]