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The map label for this gene is iaaA [H]

Identifier: 73541617

GI number: 73541617

Start: 2115287

End: 2116261

Strand: Reverse

Name: iaaA [H]

Synonym: Reut_A1928

Alternate gene names: 73541617

Gene position: 2116261-2115287 (Counterclockwise)

Preceding gene: 73541618

Following gene: 73541616

Centisome position: 55.6

GC content: 68.0

Gene sequence:

>975_bases
ATGAACCAAGCAGTCATTGCCATCCACGGCGGCGCCGGCACGATCACGCGCGCGGCCATGGATTCGGCCCGCGAACGCGA
ATACATCGAAGCGCTGGAACAGGTGCTGCTGGCAGGCCAGCGCGTGCTGGCCGGCGGCGGCACGGCCATCGACGCCGTGA
CGGAAGCTGTCCGCCTGCTGGAAGAGTGCCCGCTGTTCAACGCCGGCAAGGGCGCAGTGCTGACGCACGCCGGCACCTAC
GAACTCGATGCCGCCGTCATGGACGGTGCGACGCTCAACGCCGGCGCGGTGGCCTGCGTGTCGCGGCTGCGCAACCCGGT
ACTGGCCGCGCGCGCGGTAATGGATCACAGCGAACACGTGCTGTTTGCCGGCGAAGGCGCTGAAGCCTTTGCCGAGGCAC
ATGGCCTGGAACTGGTGTCGCGCGACTACTACTACACCAAGGCCCGCTACGAGCAATGGGAACGCGCGCGCGCCGTGCAA
GGCATGGCACTGCTCGATCATGACGCGGCCACGCTCGCCGCGCGCGCGCTGGCCGGCAAGCCCGATCCGATCGATCCGGA
CAGCAAGTTCGGCACGGTCGGCGCCGTGGCCTGCGACAGCTTCGGCAACCTAGCCGCCGCGACGTCCACGGGCGGCGTCA
CCAACAAGCAGGTCGGCCGCGTCGGCGATTCGCCGCTGATCGGCGCAGGCTGCTATGCCGACGATGTTGCTGCGGTGTCA
GCCACCGGTACCGGTGAAATGTTCATCCGCACTGTTGCAGCCCATGATGTGTCGGCACAGATGCGGTATGCGGGCCTGTC
GCTCGAGGAGTCGGCGCGCCGCGTGGTCATGGAAAAGCTGCCGGCCATTGGCGGCCGCGGCGGCCTGATCGCGGTGGACC
GTGCCGGCAATATCACGCTGCCGTTCAATACAGAGGGCATGTACCGCGGCTTTGCGCGCGCGGACGGTCCGGTGACCGTG
GCGATCTACGGCTGA

Upstream 100 bases:

>100_bases
GCACCACGGCAGCCGCACAGCCCGTGGCATCAGCCGATGTCGATGGCAAGCCCGGCGCCGACCGCCGCAACCCTTCCAAG
CACCCAAAAGCGTAGTCCCA

Downstream 100 bases:

>100_bases
CACGCCGTTTTCCATCCGCAATTCCCGCAGCAATCCCAGGGGGAGCCAGTGGCTGAACTCTCTTCTCAATCGAATATCGT
GCTGCCGCCGCAGCGTGTGG

Product: asparaginase

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MNQAVIAIHGGAGTITRAAMDSAREREYIEALEQVLLAGQRVLAGGGTAIDAVTEAVRLLEECPLFNAGKGAVLTHAGTY
ELDAAVMDGATLNAGAVACVSRLRNPVLAARAVMDHSEHVLFAGEGAEAFAEAHGLELVSRDYYYTKARYEQWERARAVQ
GMALLDHDAATLAARALAGKPDPIDPDSKFGTVGAVACDSFGNLAAATSTGGVTNKQVGRVGDSPLIGAGCYADDVAAVS
ATGTGEMFIRTVAAHDVSAQMRYAGLSLEESARRVVMEKLPAIGGRGGLIAVDRAGNITLPFNTEGMYRGFARADGPVTV
AIYG

Sequences:

>Translated_324_residues
MNQAVIAIHGGAGTITRAAMDSAREREYIEALEQVLLAGQRVLAGGGTAIDAVTEAVRLLEECPLFNAGKGAVLTHAGTY
ELDAAVMDGATLNAGAVACVSRLRNPVLAARAVMDHSEHVLFAGEGAEAFAEAHGLELVSRDYYYTKARYEQWERARAVQ
GMALLDHDAATLAARALAGKPDPIDPDSKFGTVGAVACDSFGNLAAATSTGGVTNKQVGRVGDSPLIGAGCYADDVAAVS
ATGTGEMFIRTVAAHDVSAQMRYAGLSLEESARRVVMEKLPAIGGRGGLIAVDRAGNITLPFNTEGMYRGFARADGPVTV
AIYG
>Mature_324_residues
MNQAVIAIHGGAGTITRAAMDSAREREYIEALEQVLLAGQRVLAGGGTAIDAVTEAVRLLEECPLFNAGKGAVLTHAGTY
ELDAAVMDGATLNAGAVACVSRLRNPVLAARAVMDHSEHVLFAGEGAEAFAEAHGLELVSRDYYYTKARYEQWERARAVQ
GMALLDHDAATLAARALAGKPDPIDPDSKFGTVGAVACDSFGNLAAATSTGGVTNKQVGRVGDSPLIGAGCYADDVAAVS
ATGTGEMFIRTVAAHDVSAQMRYAGLSLEESARRVVMEKLPAIGGRGGLIAVDRAGNITLPFNTEGMYRGFARADGPVTV
AIYG

Specific function: Degrades proteins damaged by L-isoaspartyl residue formation (also known as beta-Asp residues). Degrades L- isoaspartyl-containing di- and tripeptides. Acts best on iso-Asp- Leu, followed by iso-Asp-Ala, -His and to a lesser extent iso-Asp- Lys, -Phe and

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=323, Percent_Identity=36.2229102167183, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI145275200, Length=323, Percent_Identity=36.2229102167183, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI98991769, Length=275, Percent_Identity=31.6363636363636, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI285002251, Length=240, Percent_Identity=34.5833333333333, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI285002253, Length=240, Percent_Identity=32.5, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1787050, Length=324, Percent_Identity=61.1111111111111, Blast_Score=366, Evalue=1e-102,
Organism=Drosophila melanogaster, GI18921183, Length=337, Percent_Identity=34.4213649851632, Blast_Score=158, Evalue=4e-39,
Organism=Drosophila melanogaster, GI21357893, Length=268, Percent_Identity=31.7164179104478, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI221330102, Length=275, Percent_Identity=33.0909090909091, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24652135, Length=275, Percent_Identity=33.0909090909091, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24655553, Length=197, Percent_Identity=30.9644670050761, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 33584; Mature: 33584

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQAVIAIHGGAGTITRAAMDSAREREYIEALEQVLLAGQRVLAGGGTAIDAVTEAVRLL
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHH
EECPLFNAGKGAVLTHAGTYELDAAVMDGATLNAGAVACVSRLRNPVLAARAVMDHSEHV
HHCCCCCCCCCEEEEECCCEEECHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCEE
LFAGEGAEAFAEAHGLELVSRDYYYTKARYEQWERARAVQGMALLDHDAATLAARALAGK
EEECCCHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHCCHHEECCCHHHHHHHHHCCC
PDPIDPDSKFGTVGAVACDSFGNLAAATSTGGVTNKQVGRVGDSPLIGAGCYADDVAAVS
CCCCCCCCCCCCCHHHHHCCCCCEEEECCCCCCCCCHHCCCCCCCEEECCCCCCCCCEEE
ATGTGEMFIRTVAAHDVSAQMRYAGLSLEESARRVVMEKLPAIGGRGGLIAVDRAGNITL
CCCCCHHHEEEHHHHCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEE
PFNTEGMYRGFARADGPVTVAIYG
EECCCHHHHHHHCCCCCEEEEEEC
>Mature Secondary Structure
MNQAVIAIHGGAGTITRAAMDSAREREYIEALEQVLLAGQRVLAGGGTAIDAVTEAVRLL
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHH
EECPLFNAGKGAVLTHAGTYELDAAVMDGATLNAGAVACVSRLRNPVLAARAVMDHSEHV
HHCCCCCCCCCEEEEECCCEEECHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCEE
LFAGEGAEAFAEAHGLELVSRDYYYTKARYEQWERARAVQGMALLDHDAATLAARALAGK
EEECCCHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHCCHHEECCCHHHHHHHHHCCC
PDPIDPDSKFGTVGAVACDSFGNLAAATSTGGVTNKQVGRVGDSPLIGAGCYADDVAAVS
CCCCCCCCCCCCCHHHHHCCCCCEEEECCCCCCCCCHHCCCCCCCEEECCCCCCCCCEEE
ATGTGEMFIRTVAAHDVSAQMRYAGLSLEESARRVVMEKLPAIGGRGGLIAVDRAGNITL
CCCCCHHHEEEHHHHCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEE
PFNTEGMYRGFARADGPVTVAIYG
EECCCHHHHHHHCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]