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The map label for this gene is ppsA [H]

Identifier: 73541553

GI number: 73541553

Start: 2038906

End: 2041290

Strand: Reverse

Name: ppsA [H]

Synonym: Reut_A1864

Alternate gene names: 73541553

Gene position: 2041290-2038906 (Counterclockwise)

Preceding gene: 73541555

Following gene: 73541552

Centisome position: 53.63

GC content: 63.35

Gene sequence:

>2385_bases
ATGACTAACCAGGCAAATAACGGCGCCTACGTGCAGCCGTTCGAGCAGTTGCGCATGCAGGATGTGGAAACGGTCGGCGG
CAAGAATTCGTCGCTGGGCGAGATGATCTCGCAGCTGGCCGAGGCCGGCGTCCGTGTTCCCGGTGGTTTTGCCACCACCG
CGCTGGCATTCCGCGACTTCCTCGCTCACAACGACCTGACCGCCCGTATTTCGGATCGCCTGAAGGCTCTGGACGTCGAT
GACGTCAAGGCCCTGGCCGAAGCCGGTGCAGAGATCCGCCGCTGGGTCGTGGAGGCACCGTTCCAGCCGCGCCTGGAACA
GGAAGTCCGTGAGTTCTATGCACGCGCCGCCGAGCGCGAAGGCGCCGAGGCATCGTTCGCCGTGCGTTCGTCCGCGACCG
CGGAAGACCTGCCCGATGCATCGTTCGCCGGCCAGCAGGAGTCGTACCTGAACGTCAGCGGCATCGACGACGTGCTGGAC
AAGATCAAGCATGTGTTCGCGTCGCTGTACAACGACCGTGCCATTTCCTACCGCGTGCACAAGGGCTTTGCCCACGACGT
GGTCGCTCTGTCGGCCGGCGTGCAGCGCATGGTCCGTTCGGACCTGGCCGCTTCGGGCGTGATGTTCACGATCGACACCG
AGTCGGGCTTCCCGGACGTGGTCTTCATCACCTCCAGCTATGGCCTGGGCGAAACGGTGGTGCAGGGCGCCGTTAACCCG
GACGAGTTCTACGTCTTCAAGCCGACCCTGCAAGCCGGCAAGTACCCGATCATCCGCCGCTCGATCGGCTCCAAGCTGAT
CAAGATGGAGTTCACCAAGCCGGGCGAAGCTGGCCGCGTGAAGACCGTCGATGTCCCGGCCGAACTGCGCAACCGCTACT
CGATCACTGACGCTGACGTGGCGGAACTGGCCAAGTACGCCATGATCATCGAGAAGCACTACGGTCGCCCGATGGACATC
GAGTGGGGCAAGGACGGCAAGGACGGCAAGATCTACATCCTGCAGGCTCGCCCGGAAACCGTGAAGAGCCAGTCCGCCGG
CAAGGCCGAGCAGCGCTTCAAACTCAAGGGTACCGCTCCGGTGCTGACCAGCGGCCGTGCGATCGGCCAGAAGATCGGTA
CGGGTCCGGTGCGCGTCATCAACGACCCGTCCGAGATGGAACGCGTGCAGCCCGGCGACGTGCTGGTGGCCGACATGACC
GACCCGAACTGGGAGCCGGTGATGAAGCGCGCCTCGGCCATCGTCACGAACCGTGGCGGCCGTACCTGCCACGCGGCCAT
CATCGCCCGTGAACTGGGCGTGCCGGCCGTGGTTGGCTGCGGCGACGCGACCGACATCCTGAAGGACGGCACGCTGGTGA
CGGTGTCGTGCGCCGAAGGCGACGAAGGCCGCATCTATGACGGCCTGCTGGAAACGGAAGTGACCGAAGTCCAGCGCGGC
GAAATGCCGGAAATCGCCGTTAAGCTGACGATGAATGTGGGCAACCCGCAACTGGCCTTCGACTTCGCGCAGATCCCGAA
TTCCGGCGTGGGCCTGGCCCGCCTCGAGTTCATCATCAACAACAACATCGGCGTGCACCCGAAGGCCATCCTCGACTATC
CGCAAGTCGATGCCGACCTGAAGAAGGCCGTGGAAAGCGTAGCCCGCGGCCATGCCAGCCCGCGTGCGTTCTATGTTGAC
AAGCTGGCGGAAGGTATCGCCACCATTGGTGCCGCGTTCTATCCAAAGCCGGTCATCGTGCGCCTGTCGGACTTCAAGTC
GAACGAGTACAAGAAGCTGATCGGCGGCTCGCGTTACGAGCCGGATGAAGAGAACCCGATGCTGGGCTTCCGTGGCGCGT
CGCGCTACATCGCCGAAGACTTCGCTGAAGCTTTCGAGATGGAGTGCAAGGCCATGAAGCGTGTGCGCGACGAAATGGGC
TTGACCAACGTCGAGATCATGGTGCCGTTCGTGCGCACGCTGGGCCAGGCCGAGAAGGTTATCGACCTGCTCGCCAAGCA
TGGCCTGAAGCGCGGCGAGAACGGCCTGCGCGTCATCATGATGTGCGAAGTGCCGTCGAATGCCATCCTGGCCGAGCAGT
TCCTGCAGTACTTCGATGGCTTCTCGATTGGTTCGAATGACCTGACTCAGCTGACCCTGGGCCTGGACCGCGACTCCGGC
ATGGAGCTGCTGGCCAAGGACTTCGACGAGCGCGACCCCGCCGTGCGCTTCATGCTGTCGCGCGCGATCCAGGCCTGCAT
CAGCCAGAACAAGTATGTTGGCATCTGCGGCCAGGGTCCTTCGGATCATCCGGACTTCGCCGCCTGGCTGGTCAAGGAAG
GCATCAAGTCGATCTCGCTGAACCCGGATTCGGTGGTCGACACGTGGCAGCAACTGGCGTCCTGA

Upstream 100 bases:

>100_bases
GCACTGCGGGGTTGTTTAGCCAAGCGATCGGTCCGGGGACAGAACAACAAGAACATACCGGATACCCGCGTCAGTTTTCT
TACTTTGTTTTGAGGCTTTC

Downstream 100 bases:

>100_bases
TGTTTTTGACGTCACAATAACGATAACTGCCGGCGCCGCGATGCGCCGGGTAGCACCGGCCCTGCGGCAGGTGTGAACAA
GGGCCCCCGCAGGCGCCGCT

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 794; Mature: 793

Protein sequence:

>794_residues
MTNQANNGAYVQPFEQLRMQDVETVGGKNSSLGEMISQLAEAGVRVPGGFATTALAFRDFLAHNDLTARISDRLKALDVD
DVKALAEAGAEIRRWVVEAPFQPRLEQEVREFYARAAEREGAEASFAVRSSATAEDLPDASFAGQQESYLNVSGIDDVLD
KIKHVFASLYNDRAISYRVHKGFAHDVVALSAGVQRMVRSDLAASGVMFTIDTESGFPDVVFITSSYGLGETVVQGAVNP
DEFYVFKPTLQAGKYPIIRRSIGSKLIKMEFTKPGEAGRVKTVDVPAELRNRYSITDADVAELAKYAMIIEKHYGRPMDI
EWGKDGKDGKIYILQARPETVKSQSAGKAEQRFKLKGTAPVLTSGRAIGQKIGTGPVRVINDPSEMERVQPGDVLVADMT
DPNWEPVMKRASAIVTNRGGRTCHAAIIARELGVPAVVGCGDATDILKDGTLVTVSCAEGDEGRIYDGLLETEVTEVQRG
EMPEIAVKLTMNVGNPQLAFDFAQIPNSGVGLARLEFIINNNIGVHPKAILDYPQVDADLKKAVESVARGHASPRAFYVD
KLAEGIATIGAAFYPKPVIVRLSDFKSNEYKKLIGGSRYEPDEENPMLGFRGASRYIAEDFAEAFEMECKAMKRVRDEMG
LTNVEIMVPFVRTLGQAEKVIDLLAKHGLKRGENGLRVIMMCEVPSNAILAEQFLQYFDGFSIGSNDLTQLTLGLDRDSG
MELLAKDFDERDPAVRFMLSRAIQACISQNKYVGICGQGPSDHPDFAAWLVKEGIKSISLNPDSVVDTWQQLAS

Sequences:

>Translated_794_residues
MTNQANNGAYVQPFEQLRMQDVETVGGKNSSLGEMISQLAEAGVRVPGGFATTALAFRDFLAHNDLTARISDRLKALDVD
DVKALAEAGAEIRRWVVEAPFQPRLEQEVREFYARAAEREGAEASFAVRSSATAEDLPDASFAGQQESYLNVSGIDDVLD
KIKHVFASLYNDRAISYRVHKGFAHDVVALSAGVQRMVRSDLAASGVMFTIDTESGFPDVVFITSSYGLGETVVQGAVNP
DEFYVFKPTLQAGKYPIIRRSIGSKLIKMEFTKPGEAGRVKTVDVPAELRNRYSITDADVAELAKYAMIIEKHYGRPMDI
EWGKDGKDGKIYILQARPETVKSQSAGKAEQRFKLKGTAPVLTSGRAIGQKIGTGPVRVINDPSEMERVQPGDVLVADMT
DPNWEPVMKRASAIVTNRGGRTCHAAIIARELGVPAVVGCGDATDILKDGTLVTVSCAEGDEGRIYDGLLETEVTEVQRG
EMPEIAVKLTMNVGNPQLAFDFAQIPNSGVGLARLEFIINNNIGVHPKAILDYPQVDADLKKAVESVARGHASPRAFYVD
KLAEGIATIGAAFYPKPVIVRLSDFKSNEYKKLIGGSRYEPDEENPMLGFRGASRYIAEDFAEAFEMECKAMKRVRDEMG
LTNVEIMVPFVRTLGQAEKVIDLLAKHGLKRGENGLRVIMMCEVPSNAILAEQFLQYFDGFSIGSNDLTQLTLGLDRDSG
MELLAKDFDERDPAVRFMLSRAIQACISQNKYVGICGQGPSDHPDFAAWLVKEGIKSISLNPDSVVDTWQQLAS
>Mature_793_residues
TNQANNGAYVQPFEQLRMQDVETVGGKNSSLGEMISQLAEAGVRVPGGFATTALAFRDFLAHNDLTARISDRLKALDVDD
VKALAEAGAEIRRWVVEAPFQPRLEQEVREFYARAAEREGAEASFAVRSSATAEDLPDASFAGQQESYLNVSGIDDVLDK
IKHVFASLYNDRAISYRVHKGFAHDVVALSAGVQRMVRSDLAASGVMFTIDTESGFPDVVFITSSYGLGETVVQGAVNPD
EFYVFKPTLQAGKYPIIRRSIGSKLIKMEFTKPGEAGRVKTVDVPAELRNRYSITDADVAELAKYAMIIEKHYGRPMDIE
WGKDGKDGKIYILQARPETVKSQSAGKAEQRFKLKGTAPVLTSGRAIGQKIGTGPVRVINDPSEMERVQPGDVLVADMTD
PNWEPVMKRASAIVTNRGGRTCHAAIIARELGVPAVVGCGDATDILKDGTLVTVSCAEGDEGRIYDGLLETEVTEVQRGE
MPEIAVKLTMNVGNPQLAFDFAQIPNSGVGLARLEFIINNNIGVHPKAILDYPQVDADLKKAVESVARGHASPRAFYVDK
LAEGIATIGAAFYPKPVIVRLSDFKSNEYKKLIGGSRYEPDEENPMLGFRGASRYIAEDFAEAFEMECKAMKRVRDEMGL
TNVEIMVPFVRTLGQAEKVIDLLAKHGLKRGENGLRVIMMCEVPSNAILAEQFLQYFDGFSIGSNDLTQLTLGLDRDSGM
ELLAKDFDERDPAVRFMLSRAIQACISQNKYVGICGQGPSDHPDFAAWLVKEGIKSISLNPDSVVDTWQQLAS

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=792, Percent_Identity=64.8989898989899, Blast_Score=1061, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=409, Percent_Identity=24.6943765281174, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI48994992, Length=407, Percent_Identity=25.3071253071253, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1788726, Length=193, Percent_Identity=31.6062176165803, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1789193, Length=391, Percent_Identity=25.5754475703325, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17564524, Length=329, Percent_Identity=27.0516717325228, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 86802; Mature: 86670

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNQANNGAYVQPFEQLRMQDVETVGGKNSSLGEMISQLAEAGVRVPGGFATTALAFRDF
CCCCCCCCCEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
LAHNDLTARISDRLKALDVDDVKALAEAGAEIRRWVVEAPFQPRLEQEVREFYARAAERE
HHCCCHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
GAEASFAVRSSATAEDLPDASFAGQQESYLNVSGIDDVLDKIKHVFASLYNDRAISYRVH
CCCCEEEECCCCCHHCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHCCCEEEEEEC
KGFAHDVVALSAGVQRMVRSDLAASGVMFTIDTESGFPDVVFITSSYGLGETVVQGAVNP
CCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEECCCCCCHHHHHCCCCC
DEFYVFKPTLQAGKYPIIRRSIGSKLIKMEFTKPGEAGRVKTVDVPAELRNRYSITDADV
CCEEEECCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCCEEEEECCHHHHCCCCCCCHHH
AELAKYAMIIEKHYGRPMDIEWGKDGKDGKIYILQARPETVKSQSAGKAEQRFKLKGTAP
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHCCCCCCHHHEEEECCCCC
VLTSGRAIGQKIGTGPVRVINDPSEMERVQPGDVLVADMTDPNWEPVMKRASAIVTNRGG
CHHCCHHHHHHCCCCCEEEECCHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCC
RTCHAAIIARELGVPAVVGCGDATDILKDGTLVTVSCAEGDEGRIYDGLLETEVTEVQRG
CCHHHHHHHHHCCCCEEEECCCHHHHHCCCCEEEEEECCCCCCCEEHHHHHHHHHHHHCC
EMPEIAVKLTMNVGNPQLAFDFAQIPNSGVGLARLEFIINNNIGVHPKAILDYPQVDADL
CCCEEEEEEEEECCCCCEEEEHHHCCCCCCCCEEEEEEECCCCCCCCHHHCCCCCCCHHH
KKAVESVARGHASPRAFYVDKLAEGIATIGAAFYPKPVIVRLSDFKSNEYKKLIGGSRYE
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCCC
PDEENPMLGFRGASRYIAEDFAEAFEMECKAMKRVRDEMGLTNVEIMVPFVRTLGQAEKV
CCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
IDLLAKHGLKRGENGLRVIMMCEVPSNAILAEQFLQYFDGFSIGSNDLTQLTLGLDRDSG
HHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCC
MELLAKDFDERDPAVRFMLSRAIQACISQNKYVGICGQGPSDHPDFAAWLVKEGIKSISL
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCC
NPDSVVDTWQQLAS
CCHHHHHHHHHHCC
>Mature Secondary Structure 
TNQANNGAYVQPFEQLRMQDVETVGGKNSSLGEMISQLAEAGVRVPGGFATTALAFRDF
CCCCCCCCEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
LAHNDLTARISDRLKALDVDDVKALAEAGAEIRRWVVEAPFQPRLEQEVREFYARAAERE
HHCCCHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
GAEASFAVRSSATAEDLPDASFAGQQESYLNVSGIDDVLDKIKHVFASLYNDRAISYRVH
CCCCEEEECCCCCHHCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHCCCEEEEEEC
KGFAHDVVALSAGVQRMVRSDLAASGVMFTIDTESGFPDVVFITSSYGLGETVVQGAVNP
CCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEECCCCCCHHHHHCCCCC
DEFYVFKPTLQAGKYPIIRRSIGSKLIKMEFTKPGEAGRVKTVDVPAELRNRYSITDADV
CCEEEECCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCCEEEEECCHHHHCCCCCCCHHH
AELAKYAMIIEKHYGRPMDIEWGKDGKDGKIYILQARPETVKSQSAGKAEQRFKLKGTAP
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHCCCCCCHHHEEEECCCCC
VLTSGRAIGQKIGTGPVRVINDPSEMERVQPGDVLVADMTDPNWEPVMKRASAIVTNRGG
CHHCCHHHHHHCCCCCEEEECCHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCC
RTCHAAIIARELGVPAVVGCGDATDILKDGTLVTVSCAEGDEGRIYDGLLETEVTEVQRG
CCHHHHHHHHHCCCCEEEECCCHHHHHCCCCEEEEEECCCCCCCEEHHHHHHHHHHHHCC
EMPEIAVKLTMNVGNPQLAFDFAQIPNSGVGLARLEFIINNNIGVHPKAILDYPQVDADL
CCCEEEEEEEEECCCCCEEEEHHHCCCCCCCCEEEEEEECCCCCCCCHHHCCCCCCCHHH
KKAVESVARGHASPRAFYVDKLAEGIATIGAAFYPKPVIVRLSDFKSNEYKKLIGGSRYE
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCCC
PDEENPMLGFRGASRYIAEDFAEAFEMECKAMKRVRDEMGLTNVEIMVPFVRTLGQAEKV
CCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
IDLLAKHGLKRGENGLRVIMMCEVPSNAILAEQFLQYFDGFSIGSNDLTQLTLGLDRDSG
HHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCC
MELLAKDFDERDPAVRFMLSRAIQACISQNKYVGICGQGPSDHPDFAAWLVKEGIKSISL
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCC
NPDSVVDTWQQLAS
CCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]