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The map label for this gene is artJ [H]

Identifier: 73541411

GI number: 73541411

Start: 1860446

End: 1861489

Strand: Reverse

Name: artJ [H]

Synonym: Reut_A1722

Alternate gene names: 73541411

Gene position: 1861489-1860446 (Counterclockwise)

Preceding gene: 73541412

Following gene: 73541410

Centisome position: 48.9

GC content: 66.57

Gene sequence:

>1044_bases
ATGAAACCGAGCGGATTTCGCCCCATGTCATTTGCTGACTTTCCCGTTGCGCCGACGCTGTCACGCGCATTCTCTTCGCG
CCTGAGCCAAGCCGCCGCGAGGCTGCGCTCACCGCTGGCCTGCTTGCTGGCCGCAGTGGCGGTCTGCCTGCCGGTAGCGG
CGGCGGCCGCGGGCACGCCGGCCGCCGCCCGCCCGGTGCCGGCAAGCACGTCGGCTAATGCGTCGGCCAATGCGTCGGCC
AGCACGTCGACTACCGCCGCATCACCGGTGGTGACCTCCACCGGCCTGGTGCAGATGCCTGACGGCCGCTTGCTGGCGCC
GGAGTTCGCCCGTATCTACGAGCGCGGCGAACTGGTGGTGGCGGTGCTCAAGGTCGACCAACCTCCGTTCTTCCAGGAGC
GCGACGGCCAGTTGCTTGGGCTCGATATCGACCTGGCCCGTGAACTGGCGCAGAAGCTGAATGTCCGGCTGCGCTTTAAC
CGCGATGCCACCACGTTCGACGGTGTGGTCAGCCTGCTGGCGCAGGGCAAGGCCGATATGGCCGTCAGCAAGCTGTCGCG
TACGCTGCCACGCACCCAGATCATCAGCTTCAGCACGCCTTACCTGCGCCTGAACCGCGCGCTGCTGCTCAACCGCGTCA
AGTTCGCCCAGATGGCGCGCGGACGGCCGGTGCCCGACGTCATCCGCGCCTATGACAGCTCGATCGGCGTGGTCGGCAAT
TCGTCCTATTCCGAATACGTGACAAGCAACTTCCCCAAGGCCTTTGTCACCCGTTACCCGACCTGGGACAGCGCGCTCAA
GGCGCTCGACGCCGGCCAAGTCACCGCGGTCTACCGCGACGAATTCGAGGTCAAGCGCGTGCTCAAGGCCGACCCCACCG
CGGCGCTGCGCCTGCGCGTAGTGACGCTGCACGATCTCGAGGACACGCTCGGCATTGCCGTTAACGTGTCTGCGCCGGCG
CTGCTGGCCTTTGTCAACCAGTTCCTGGCGGAGCGGCCCAAGCACCTCGAAGTGGCTGATGTGCTCGAAGCCGCCGCGCA
ATAA

Upstream 100 bases:

>100_bases
AACCGGTGCCGGAGTCAGACCAGATGCCCGCGCAGGCACAGACCAGCGCGCCCGGCGCTGTCGCCGAGGTGGCGACGGTT
GCCAGCCCCCCCAAGATTCA

Downstream 100 bases:

>100_bases
ACACCAGGAGAGAAACCAAGATGAACACGACCAAGCTATATGCCTTTGCCCTCAACCCGTGGGTCGTGATCGGGAGCCTG
ACGCTGGGCGGCGCCATGGG

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MKPSGFRPMSFADFPVAPTLSRAFSSRLSQAAARLRSPLACLLAAVAVCLPVAAAAAGTPAAARPVPASTSANASANASA
STSTTAASPVVTSTGLVQMPDGRLLAPEFARIYERGELVVAVLKVDQPPFFQERDGQLLGLDIDLARELAQKLNVRLRFN
RDATTFDGVVSLLAQGKADMAVSKLSRTLPRTQIISFSTPYLRLNRALLLNRVKFAQMARGRPVPDVIRAYDSSIGVVGN
SSYSEYVTSNFPKAFVTRYPTWDSALKALDAGQVTAVYRDEFEVKRVLKADPTAALRLRVVTLHDLEDTLGIAVNVSAPA
LLAFVNQFLAERPKHLEVADVLEAAAQ

Sequences:

>Translated_347_residues
MKPSGFRPMSFADFPVAPTLSRAFSSRLSQAAARLRSPLACLLAAVAVCLPVAAAAAGTPAAARPVPASTSANASANASA
STSTTAASPVVTSTGLVQMPDGRLLAPEFARIYERGELVVAVLKVDQPPFFQERDGQLLGLDIDLARELAQKLNVRLRFN
RDATTFDGVVSLLAQGKADMAVSKLSRTLPRTQIISFSTPYLRLNRALLLNRVKFAQMARGRPVPDVIRAYDSSIGVVGN
SSYSEYVTSNFPKAFVTRYPTWDSALKALDAGQVTAVYRDEFEVKRVLKADPTAALRLRVVTLHDLEDTLGIAVNVSAPA
LLAFVNQFLAERPKHLEVADVLEAAAQ
>Mature_347_residues
MKPSGFRPMSFADFPVAPTLSRAFSSRLSQAAARLRSPLACLLAAVAVCLPVAAAAAGTPAAARPVPASTSANASANASA
STSTTAASPVVTSTGLVQMPDGRLLAPEFARIYERGELVVAVLKVDQPPFFQERDGQLLGLDIDLARELAQKLNVRLRFN
RDATTFDGVVSLLAQGKADMAVSKLSRTLPRTQIISFSTPYLRLNRALLLNRVKFAQMARGRPVPDVIRAYDSSIGVVGN
SSYSEYVTSNFPKAFVTRYPTWDSALKALDAGQVTAVYRDEFEVKRVLKADPTAALRLRVVTLHDLEDTLGIAVNVSAPA
LLAFVNQFLAERPKHLEVADVLEAAAQ

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=207, Percent_Identity=26.5700483091787, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788228, Length=231, Percent_Identity=25.974025974026, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 37196; Mature: 37196

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSGFRPMSFADFPVAPTLSRAFSSRLSQAAARLRSPLACLLAAVAVCLPVAAAAAGTP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCC
AAARPVPASTSANASANASASTSTTAASPVVTSTGLVQMPDGRLLAPEFARIYERGELVV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCEEECCCCCEECHHHHHHHHCCCEEE
AVLKVDQPPFFQERDGQLLGLDIDLARELAQKLNVRLRFNRDATTFDGVVSLLAQGKADM
EEEECCCCCCCCCCCCCEEEECHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHCCCHHH
AVSKLSRTLPRTQIISFSTPYLRLNRALLLNRVKFAQMARGRPVPDVIRAYDSSIGVVGN
HHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEC
SSYSEYVTSNFPKAFVTRYPTWDSALKALDAGQVTAVYRDEFEVKRVLKADPTAALRLRV
CCHHHHHHCCCCHHHHCCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCEEEEEE
VTLHDLEDTLGIAVNVSAPALLAFVNQFLAERPKHLEVADVLEAAAQ
EEEECCHHHHCEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MKPSGFRPMSFADFPVAPTLSRAFSSRLSQAAARLRSPLACLLAAVAVCLPVAAAAAGTP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCC
AAARPVPASTSANASANASASTSTTAASPVVTSTGLVQMPDGRLLAPEFARIYERGELVV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCEEECCCCCEECHHHHHHHHCCCEEE
AVLKVDQPPFFQERDGQLLGLDIDLARELAQKLNVRLRFNRDATTFDGVVSLLAQGKADM
EEEECCCCCCCCCCCCCEEEECHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHCCCHHH
AVSKLSRTLPRTQIISFSTPYLRLNRALLLNRVKFAQMARGRPVPDVIRAYDSSIGVVGN
HHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEC
SSYSEYVTSNFPKAFVTRYPTWDSALKALDAGQVTAVYRDEFEVKRVLKADPTAALRLRV
CCHHHHHHCCCCHHHHCCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCEEEEEE
VTLHDLEDTLGIAVNVSAPALLAFVNQFLAERPKHLEVADVLEAAAQ
EEEECCHHHHCEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]