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The map label for this gene is caiD [H]

Identifier: 73541357

GI number: 73541357

Start: 1799352

End: 1800116

Strand: Reverse

Name: caiD [H]

Synonym: Reut_A1667

Alternate gene names: 73541357

Gene position: 1800116-1799352 (Counterclockwise)

Preceding gene: 73541358

Following gene: 73541355

Centisome position: 47.29

GC content: 67.58

Gene sequence:

>765_bases
ATGAGCGACACGCTGCAACTTGAAACGCGCGGCAATATCCTGCTGATCACGATGAACCGGCCCGAGGCGCGCAACGCCAT
GGACTTCGAGACTGCGACGGCGCTGGCCGCCGCGCTCGACCAGCTCGAGTCGCGCGACGACCTGAGCGTGGCCATCCTGA
CCGGTGCGGGCGGTACCTTCTGCTCCGGCATGGACCTGAAGGGCTTCCTGGCCGGCAAGCGCCCCAGCCTGCCGGGCCGC
GGCTTTGGCGGCCTGACCGAAGCGCAGCCGCGCAAGGTACTGATCGCCGCGGTGGAAGGCTATGCACTGGCCGGTGGCTT
TGAAATGGCGCTGGCCTGTGACCTGATCGTGGCCTCGAAGGCGGCCAAGTTCGGCCTGCCCGAAGTCAAGCGCGGCCTGG
TGGCTGCCGCTGGCGGCCTGATGCGCCTGCCGAGCCGGATCCCGTACCACATCGCCATGGAATACGCGCTGACCGGCAAT
ATGCTCGGCGCCGAACAGGCGCATACGTACGGGCTGGTCAATCGCCTGACCGAGCCGGGCCAGGCACTGGAAGCCGCGAT
CGCGCTGGCCGAGGAAGTCGGTGCCAATGGTCCGATGGCCGTGGCGGTCAGCAAGCAGATCGTCAGCGAGTCCGCTGACT
GGTCGGCGGCCGAGATGTTCGAGCGCCAGCGTCCGCTTGTCGCCCCGGTGTTCACGTCATCCGATGCCCGCGAGGGCGCC
GCTGCGTTTGCCGAGAAGCGCAAACCTGTGTGGACCGGCAAGTAA

Upstream 100 bases:

>100_bases
TCAACGGCGAAACCATTCGGCTGGATGGCGCTATTCGCATGGCCCCGCGCTGAGCTGCCGGTTTCTCTTCAATACCGATT
CAAGCAACACAAGGACAAGC

Downstream 100 bases:

>100_bases
GCCGCTGAAGCGGCAAGCCCTTGCCCATGCGGCCGGCCCTTGCGCCGGCCGCATTGCCATCCACGCTCATCCGAAAAGAA
GCCGCCTATCGGCGCACGCT

Product: enoyl-CoA hydratase

Products: L-Carnitine.; L-carnitinyl-CoA [C]

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MSDTLQLETRGNILLITMNRPEARNAMDFETATALAAALDQLESRDDLSVAILTGAGGTFCSGMDLKGFLAGKRPSLPGR
GFGGLTEAQPRKVLIAAVEGYALAGGFEMALACDLIVASKAAKFGLPEVKRGLVAAAGGLMRLPSRIPYHIAMEYALTGN
MLGAEQAHTYGLVNRLTEPGQALEAAIALAEEVGANGPMAVAVSKQIVSESADWSAAEMFERQRPLVAPVFTSSDAREGA
AAFAEKRKPVWTGK

Sequences:

>Translated_254_residues
MSDTLQLETRGNILLITMNRPEARNAMDFETATALAAALDQLESRDDLSVAILTGAGGTFCSGMDLKGFLAGKRPSLPGR
GFGGLTEAQPRKVLIAAVEGYALAGGFEMALACDLIVASKAAKFGLPEVKRGLVAAAGGLMRLPSRIPYHIAMEYALTGN
MLGAEQAHTYGLVNRLTEPGQALEAAIALAEEVGANGPMAVAVSKQIVSESADWSAAEMFERQRPLVAPVFTSSDAREGA
AAFAEKRKPVWTGK
>Mature_253_residues
SDTLQLETRGNILLITMNRPEARNAMDFETATALAAALDQLESRDDLSVAILTGAGGTFCSGMDLKGFLAGKRPSLPGRG
FGGLTEAQPRKVLIAAVEGYALAGGFEMALACDLIVASKAAKFGLPEVKRGLVAAAGGLMRLPSRIPYHIAMEYALTGNM
LGAEQAHTYGLVNRLTEPGQALEAAIALAEEVGANGPMAVAVSKQIVSESADWSAAEMFERQRPLVAPVFTSSDAREGAA
AFAEKRKPVWTGK

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=248, Percent_Identity=34.2741935483871, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI4502327, Length=265, Percent_Identity=30.9433962264151, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI150378541, Length=246, Percent_Identity=32.520325203252, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI31542718, Length=247, Percent_Identity=25.5060728744939, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI70995211, Length=225, Percent_Identity=32, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI20127408, Length=184, Percent_Identity=28.2608695652174, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI68989263, Length=181, Percent_Identity=29.2817679558011, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI221142681, Length=265, Percent_Identity=43.3962264150943, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI1787659, Length=252, Percent_Identity=34.1269841269841, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1787660, Length=252, Percent_Identity=32.1428571428571, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87082183, Length=255, Percent_Identity=28.2352941176471, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1788682, Length=202, Percent_Identity=34.1584158415842, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1788597, Length=244, Percent_Identity=29.5081967213115, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1790281, Length=242, Percent_Identity=29.3388429752066, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17554946, Length=247, Percent_Identity=34.412955465587, Blast_Score=150, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI25145438, Length=243, Percent_Identity=35.3909465020576, Blast_Score=147, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17560910, Length=255, Percent_Identity=31.7647058823529, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17540714, Length=225, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17536985, Length=264, Percent_Identity=24.6212121212121, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=235, Percent_Identity=25.1063829787234, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=243, Percent_Identity=25.9259259259259, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17558304, Length=188, Percent_Identity=27.6595744680851, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI25144276, Length=228, Percent_Identity=28.0701754385965, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17508951, Length=228, Percent_Identity=28.0701754385965, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17508953, Length=228, Percent_Identity=28.0701754385965, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI20129971, Length=245, Percent_Identity=36.3265306122449, Blast_Score=153, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24653477, Length=245, Percent_Identity=36.3265306122449, Blast_Score=153, Evalue=1e-37,
Organism=Drosophila melanogaster, GI21357171, Length=190, Percent_Identity=37.3684210526316, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24653139, Length=256, Percent_Identity=33.984375, Blast_Score=121, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24650670, Length=202, Percent_Identity=37.6237623762376, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI19922422, Length=259, Percent_Identity=27.7992277992278, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI19920382, Length=260, Percent_Identity=26.1538461538462, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24583077, Length=184, Percent_Identity=30.9782608695652, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24583079, Length=184, Percent_Identity=30.9782608695652, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19921000, Length=184, Percent_Identity=30.9782608695652, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24654903, Length=192, Percent_Identity=25, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24583165, Length=212, Percent_Identity=24.5283018867925, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.- [C]

Molecular weight: Translated: 26653; Mature: 26522

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTLQLETRGNILLITMNRPEARNAMDFETATALAAALDQLESRDDLSVAILTGAGGTF
CCCCEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCE
CSGMDLKGFLAGKRPSLPGRGFGGLTEAQPRKVLIAAVEGYALAGGFEMALACDLIVASK
ECCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCEEEECCHHHHHHHHHHHHHH
AAKFGLPEVKRGLVAAAGGLMRLPSRIPYHIAMEYALTGNMLGAEQAHTYGLVNRLTEPG
HHHCCCHHHHHHHHHHCCCHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCH
QALEAAIALAEEVGANGPMAVAVSKQIVSESADWSAAEMFERQRPLVAPVFTSSDAREGA
HHHHHHHHHHHHHCCCCCEEEEEHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCCCHHHH
AAFAEKRKPVWTGK
HHHHHHCCCCCCCC
>Mature Secondary Structure 
SDTLQLETRGNILLITMNRPEARNAMDFETATALAAALDQLESRDDLSVAILTGAGGTF
CCCEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCE
CSGMDLKGFLAGKRPSLPGRGFGGLTEAQPRKVLIAAVEGYALAGGFEMALACDLIVASK
ECCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCEEEECCHHHHHHHHHHHHHH
AAKFGLPEVKRGLVAAAGGLMRLPSRIPYHIAMEYALTGNMLGAEQAHTYGLVNRLTEPG
HHHCCCHHHHHHHHHHCCCHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCH
QALEAAIALAEEVGANGPMAVAVSKQIVSESADWSAAEMFERQRPLVAPVFTSSDAREGA
HHHHHHHHHHHHHCCCCCEEEEEHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCCCHHHH
AAFAEKRKPVWTGK
HHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Carnitine; D-carnitinyl-CoA [C]

Specific reaction: D-Carnitine = L-Carnitine. D-carnitinyl-CoA = L-carnitinyl-CoA [C]

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA