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The map label for this gene is glnH [H]

Identifier: 73541284

GI number: 73541284

Start: 1715962

End: 1716720

Strand: Reverse

Name: glnH [H]

Synonym: Reut_A1594

Alternate gene names: 73541284

Gene position: 1716720-1715962 (Counterclockwise)

Preceding gene: 73541285

Following gene: 73541283

Centisome position: 45.1

GC content: 64.43

Gene sequence:

>759_bases
ATGAACCTTCGCCGCAAATGCCTGATGGCCGGCCTCACGGCCCTGATCGGCCTGACTTTTGGCGCCGCCGCCCACGCCGA
GGACAATGCCGTGCTGCGTGTGGCCACCGACGCCACCTTCCCGCCGATGGAATTTATCGAGAACGGCAAGCGCACCGGCT
TTGACGTGGAACTCGTGGAAGCCATGGCCAAGACGATGGGCAAGCGGGTCGAATGGACCGACATCGACTTCAAGGGCCTG
ATTCCCGGGCTGGTTTCGCGCCGCTTCGACATGGCCGTGTCCGGCATCTACATCACCCCCGAGCGCCGCAAGGTGGTGGA
CTTCACCGAGCCGTACTACACCGGCGGCCTGGTCGCGCTCGTCAAGAGCGGCAACACCACCATCCACGCCCCGGCCGACC
TGAACGGCAAGAAGGTCAGCGTGCAGGTGGGCACCAAGTCGGTCAGCTTCCTGCGCGACACGTATCCGCAGGTGCAACGC
GTGGAAGTGGAAAAGAACCAGGAAATGTTCAACCTGGTCGAGATCGGCCGTGCCGACGCCGCCGTGACCGGCAAGCCGGC
CGCGATGCAATACGTCAAGACCCGCGGCGGCCTGCGCATGGTCGAGCAACCGCTCACCACCGAGGAATACGGCATGGCCG
TGCGCAAGGACATGCCCGAGCTCACCCGTGCCCTCAATGCGGCGCTCGTCAAAGTGAAGGCCGATGGCACCTACGCCAAG
CTGACCCAGAAGTGGTTCGGCACGGCCGCCTCCAAGTAA

Upstream 100 bases:

>100_bases
GCGCCTGCAGTCCTACTGAATTACGAAGCCCCTCATTACCGCTGCCGTCGGCAAGCCCATGTGGCGGCAGATTCCTCGTT
CCCGAGAAAAGGAGACCTCC

Downstream 100 bases:

>100_bases
CCCGAGCCGGTCATCATCATGGAACTGGATTTTTCTCCCGTCTTCAGCGGCTGGCCTGACATCGTCCACGGTGCCATCGT
CACCGTGGAAGTGACAGCCT

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: GlnBP [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MNLRRKCLMAGLTALIGLTFGAAAHAEDNAVLRVATDATFPPMEFIENGKRTGFDVELVEAMAKTMGKRVEWTDIDFKGL
IPGLVSRRFDMAVSGIYITPERRKVVDFTEPYYTGGLVALVKSGNTTIHAPADLNGKKVSVQVGTKSVSFLRDTYPQVQR
VEVEKNQEMFNLVEIGRADAAVTGKPAAMQYVKTRGGLRMVEQPLTTEEYGMAVRKDMPELTRALNAALVKVKADGTYAK
LTQKWFGTAASK

Sequences:

>Translated_252_residues
MNLRRKCLMAGLTALIGLTFGAAAHAEDNAVLRVATDATFPPMEFIENGKRTGFDVELVEAMAKTMGKRVEWTDIDFKGL
IPGLVSRRFDMAVSGIYITPERRKVVDFTEPYYTGGLVALVKSGNTTIHAPADLNGKKVSVQVGTKSVSFLRDTYPQVQR
VEVEKNQEMFNLVEIGRADAAVTGKPAAMQYVKTRGGLRMVEQPLTTEEYGMAVRKDMPELTRALNAALVKVKADGTYAK
LTQKWFGTAASK
>Mature_252_residues
MNLRRKCLMAGLTALIGLTFGAAAHAEDNAVLRVATDATFPPMEFIENGKRTGFDVELVEAMAKTMGKRVEWTDIDFKGL
IPGLVSRRFDMAVSGIYITPERRKVVDFTEPYYTGGLVALVKSGNTTIHAPADLNGKKVSVQVGTKSVSFLRDTYPQVQR
VEVEKNQEMFNLVEIGRADAAVTGKPAAMQYVKTRGGLRMVEQPLTTEEYGMAVRKDMPELTRALNAALVKVKADGTYAK
LTQKWFGTAASK

Specific function: Involved in a glutamine-transport system glnHPQ [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=249, Percent_Identity=36.9477911646586, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1787085, Length=248, Percent_Identity=35.4838709677419, Blast_Score=150, Evalue=6e-38,
Organism=Escherichia coli, GI1788228, Length=249, Percent_Identity=32.9317269076305, Blast_Score=137, Evalue=5e-34,
Organism=Escherichia coli, GI1787088, Length=248, Percent_Identity=35.4838709677419, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI1788649, Length=254, Percent_Identity=29.5275590551181, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1788648, Length=227, Percent_Identity=29.9559471365639, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1786876, Length=236, Percent_Identity=25.8474576271186, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27656; Mature: 27656

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLRRKCLMAGLTALIGLTFGAAAHAEDNAVLRVATDATFPPMEFIENGKRTGFDVELVE
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHCCCCCCCHHHHHH
AMAKTMGKRVEWTDIDFKGLIPGLVSRRFDMAVSGIYITPERRKVVDFTEPYYTGGLVAL
HHHHHHCCCCEEECCCHHHHHHHHHHHHHHHEEEEEEECCCCCEEEECCCCCCCCCEEEE
VKSGNTTIHAPADLNGKKVSVQVGTKSVSFLRDTYPQVQRVEVEKNQEMFNLVEIGRADA
EECCCEEEECCCCCCCCEEEEEECCCHHHHHHHCCCHHEEEEHHCCHHHHHHHHHCCCCC
AVTGKPAAMQYVKTRGGLRMVEQPLTTEEYGMAVRKDMPELTRALNAALVKVKADGTYAK
CCCCCHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHEEEEEECCCCHHH
LTQKWFGTAASK
HHHHHHHHCCCC
>Mature Secondary Structure
MNLRRKCLMAGLTALIGLTFGAAAHAEDNAVLRVATDATFPPMEFIENGKRTGFDVELVE
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHCCCCCCCHHHHHH
AMAKTMGKRVEWTDIDFKGLIPGLVSRRFDMAVSGIYITPERRKVVDFTEPYYTGGLVAL
HHHHHHCCCCEEECCCHHHHHHHHHHHHHHHEEEEEEECCCCCEEEECCCCCCCCCEEEE
VKSGNTTIHAPADLNGKKVSVQVGTKSVSFLRDTYPQVQRVEVEKNQEMFNLVEIGRADA
EECCCEEEECCCCCCCCEEEEEECCCHHHHHHHCCCHHEEEEHHCCHHHHHHHHHCCCCC
AVTGKPAAMQYVKTRGGLRMVEQPLTTEEYGMAVRKDMPELTRALNAALVKVKADGTYAK
CCCCCHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHEEEEEECCCCHHH
LTQKWFGTAASK
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]