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The map label for this gene is 73541275

Identifier: 73541275

GI number: 73541275

Start: 1707954

End: 1709507

Strand: Direct

Name: 73541275

Synonym: Reut_A1585

Alternate gene names: NA

Gene position: 1707954-1709507 (Clockwise)

Preceding gene: 73541274

Following gene: 73541276

Centisome position: 44.87

GC content: 62.16

Gene sequence:

>1554_bases
ATGATTCGCACTGGCAAGCAGTACCTGGAATCGCTGAACGACGGCCGCAACGTATGGGTCGGCAACGAGAAGATCGACAA
CGTCGCGACGCACCCCAAGACGCGGGACTACGCGCAGCGTCACGCCGACTTCTACGACCTGCACCATCGTCCCGACCTGC
AGGACGTAATGACCTTCGTCGACAAGGATGGCGAGCGCCGCACCATGCAGTGGTTCGGCCACTACGACAAGGAACAGCTG
CGCCGCAAACGCAAGTATCACGAGACGATCATGCGCGAGATGGCCGGCGCGTCGTTCCCGCGCACGCCGGACGTCAACAA
CTACGTGCTGCAGACCTATATCGACGATCCGTCGCCGTGGGAAACGCAGACCATCGGCGCCGAAGGCAAGGTGAAGGCGA
AGAACATCGTCGACTTCGTCAATTTCGCCAAGAAGCACGACCTGAACTGCGCGCCGCAGTTCGTCGATCCGCAGATGGAT
CGCTCCAACCCCGATGCCCAGCAGCGTTCGCCGGGGCTGCGCGTGATCGAGAAGAACGACAAGGGCATCGTCGTGTCAGG
GGTCAAGGCTATCGGCACTGGCGTGGCGTTTGCCGACTGGATTCATATCGGGGTGTTCTTCCGCCCCGGTATCCCGGGCG
ACCAGATCATCTTCGCGGCCACGCCGGTCAACACCCCGGGCGTGACCATCGTCTGCCGCGAGAGCGTGGTCAAGGAAGAT
CCCATCGAACACCCTCTGGCCTCGCAGGGCGATGAGCTGGACGGCATGACGGTATTCGACAACGTTTTCATCCCGTGGTC
GCACGTGTTCCATTTGGGCAATCCGGAACACGCCAAGCTCTACCCGCAGCGTGTGTTCGACTGGCTGCACTATCACGCGC
TGATTCGCCAGTCGGTGCGCGCCGAGCTGATGGCTGGCCTGGCGATCCTGATCACCGAGCATATCGGCACCAACAAGATC
CCCGCGGTGCAGACGCGCGTGGCCAAGCTGATCGGCTTCCACCAGGCCATGCTCGCGCACATCGTCGCGAGCGAGGAACT
GGGCTTCCATACGCCGGGCGGGGCCTACAAGCCGAACATCCTGATCTACGACTTCGGCCGGGCGCTTTACCTCGAAAACT
TCTCGCAGATGATCTACGAGCTGGTCGATCTCTCCGGGCGCAGTGCGCTGATCTTCGCGAGCGAAGACCAATGGAACGAC
GAGGCCCTCAACGGCTGGTTCGAACGCATGAACAATGGTCCTGTGGGCCAGCCGCATGACCGCGTGAAGATCGGCCGTGT
GATCCGCGACCTGTTCCTGACCGACTGGGGCAACCGCCTGTTCGTCTTCGAGAACTTCAACGGCACGCCGCTGCAGGCCA
TCCGCATGCTGACTATGCAACGCGCCGAGTTTTCCGCTGCTGGTCCCTACGGCACGCTGGCGCGCAAGGTCTGCGGCATC
GAACTGACCGAGGGCCACGACAGCGAATACAAGGCCACGGCCGGCTACGCCCAGGCTCTGGATTCCGCGCGTCATCAGGA
AAAGCTTGCCCTGAGCGGCACGATGACTGTGTGA

Upstream 100 bases:

>100_bases
GGCCCGCGCCAGGCGAGAACCCGATTTCGTATCCGTGTCCATATGCCCTTCTCCAGGGCAGGGACTGTCACACCCCGAAA
ACCACCAAGGAGACCTGGCG

Downstream 100 bases:

>100_bases
CCGCCTCAACCTCCGGCCAGCGTCGTCTGGCCGGAACACCCGAAACATGCAAGGAGTGAATGATGCAAACCAATGAACTG
CTTGACAAGATCTTCCGCGG

Product: 4-hydroxyphenylacetate 3-hydroxylase

Products: NA

Alternate protein names: 4-HPA 3-hydroxylase; 4-HPA 3-monooxygenase large component [H]

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MIRTGKQYLESLNDGRNVWVGNEKIDNVATHPKTRDYAQRHADFYDLHHRPDLQDVMTFVDKDGERRTMQWFGHYDKEQL
RRKRKYHETIMREMAGASFPRTPDVNNYVLQTYIDDPSPWETQTIGAEGKVKAKNIVDFVNFAKKHDLNCAPQFVDPQMD
RSNPDAQQRSPGLRVIEKNDKGIVVSGVKAIGTGVAFADWIHIGVFFRPGIPGDQIIFAATPVNTPGVTIVCRESVVKED
PIEHPLASQGDELDGMTVFDNVFIPWSHVFHLGNPEHAKLYPQRVFDWLHYHALIRQSVRAELMAGLAILITEHIGTNKI
PAVQTRVAKLIGFHQAMLAHIVASEELGFHTPGGAYKPNILIYDFGRALYLENFSQMIYELVDLSGRSALIFASEDQWND
EALNGWFERMNNGPVGQPHDRVKIGRVIRDLFLTDWGNRLFVFENFNGTPLQAIRMLTMQRAEFSAAGPYGTLARKVCGI
ELTEGHDSEYKATAGYAQALDSARHQEKLALSGTMTV

Sequences:

>Translated_517_residues
MIRTGKQYLESLNDGRNVWVGNEKIDNVATHPKTRDYAQRHADFYDLHHRPDLQDVMTFVDKDGERRTMQWFGHYDKEQL
RRKRKYHETIMREMAGASFPRTPDVNNYVLQTYIDDPSPWETQTIGAEGKVKAKNIVDFVNFAKKHDLNCAPQFVDPQMD
RSNPDAQQRSPGLRVIEKNDKGIVVSGVKAIGTGVAFADWIHIGVFFRPGIPGDQIIFAATPVNTPGVTIVCRESVVKED
PIEHPLASQGDELDGMTVFDNVFIPWSHVFHLGNPEHAKLYPQRVFDWLHYHALIRQSVRAELMAGLAILITEHIGTNKI
PAVQTRVAKLIGFHQAMLAHIVASEELGFHTPGGAYKPNILIYDFGRALYLENFSQMIYELVDLSGRSALIFASEDQWND
EALNGWFERMNNGPVGQPHDRVKIGRVIRDLFLTDWGNRLFVFENFNGTPLQAIRMLTMQRAEFSAAGPYGTLARKVCGI
ELTEGHDSEYKATAGYAQALDSARHQEKLALSGTMTV
>Mature_517_residues
MIRTGKQYLESLNDGRNVWVGNEKIDNVATHPKTRDYAQRHADFYDLHHRPDLQDVMTFVDKDGERRTMQWFGHYDKEQL
RRKRKYHETIMREMAGASFPRTPDVNNYVLQTYIDDPSPWETQTIGAEGKVKAKNIVDFVNFAKKHDLNCAPQFVDPQMD
RSNPDAQQRSPGLRVIEKNDKGIVVSGVKAIGTGVAFADWIHIGVFFRPGIPGDQIIFAATPVNTPGVTIVCRESVVKED
PIEHPLASQGDELDGMTVFDNVFIPWSHVFHLGNPEHAKLYPQRVFDWLHYHALIRQSVRAELMAGLAILITEHIGTNKI
PAVQTRVAKLIGFHQAMLAHIVASEELGFHTPGGAYKPNILIYDFGRALYLENFSQMIYELVDLSGRSALIFASEDQWND
EALNGWFERMNNGPVGQPHDRVKIGRVIRDLFLTDWGNRLFVFENFNGTPLQAIRMLTMQRAEFSAAGPYGTLARKVCGI
ELTEGHDSEYKATAGYAQALDSARHQEKLALSGTMTV

Specific function: Utilizes FADH(2) supplied by hpaC, to catalyze the hydroxylation of 4-hydroxyphenylacetic acid, leading to the production of 3,4-dihydroxyphenylacetic acid (DHPA) [H]

COG id: COG2368

COG function: function code Q; Aromatic ring hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FADH(2)-utilizing monooxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR009100
- InterPro:   IPR004925
- InterPro:   IPR012687 [H]

Pfam domain/function: PF03241 HpaB [H]

EC number: NA

Molecular weight: Translated: 58471; Mature: 58471

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRTGKQYLESLNDGRNVWVGNEKIDNVATHPKTRDYAQRHADFYDLHHRPDLQDVMTFV
CCCCHHHHHHHHCCCCEEEECCCHHCHHHCCCCCHHHHHHCCCHHHCCCCCCHHHHHHHH
DKDGERRTMQWFGHYDKEQLRRKRKYHETIMREMAGASFPRTPDVNNYVLQTYIDDPSPW
CCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCC
ETQTIGAEGKVKAKNIVDFVNFAKKHDLNCAPQFVDPQMDRSNPDAQQRSPGLRVIEKND
CCEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCEEEEECC
KGIVVSGVKAIGTGVAFADWIHIGVFFRPGIPGDQIIFAATPVNTPGVTIVCRESVVKED
CEEEEECHHHHHCCHHHHHHHEEEEEEECCCCCCCEEEEECCCCCCCEEEEECCHHHCCC
PIEHPLASQGDELDGMTVFDNVFIPWSHVFHLGNPEHAKLYPQRVFDWLHYHALIRQSVR
CCCCCCCCCCCCCCCEEEECCEECCHHHEEECCCCCHHHCCHHHHHHHHHHHHHHHHHHH
AELMAGLAILITEHIGTNKIPAVQTRVAKLIGFHQAMLAHIVASEELGFHTPGGAYKPNI
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCE
LIYDFGRALYLENFSQMIYELVDLSGRSALIFASEDQWNDEALNGWFERMNNGPVGQPHD
EEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHH
RVKIGRVIRDLFLTDWGNRLFVFENFNGTPLQAIRMLTMQRAEFSAAGPYGTLARKVCGI
HHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
ELTEGHDSEYKATAGYAQALDSARHQEKLALSGTMTV
EEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIRTGKQYLESLNDGRNVWVGNEKIDNVATHPKTRDYAQRHADFYDLHHRPDLQDVMTFV
CCCCHHHHHHHHCCCCEEEECCCHHCHHHCCCCCHHHHHHCCCHHHCCCCCCHHHHHHHH
DKDGERRTMQWFGHYDKEQLRRKRKYHETIMREMAGASFPRTPDVNNYVLQTYIDDPSPW
CCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCC
ETQTIGAEGKVKAKNIVDFVNFAKKHDLNCAPQFVDPQMDRSNPDAQQRSPGLRVIEKND
CCEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCEEEEECC
KGIVVSGVKAIGTGVAFADWIHIGVFFRPGIPGDQIIFAATPVNTPGVTIVCRESVVKED
CEEEEECHHHHHCCHHHHHHHEEEEEEECCCCCCCEEEEECCCCCCCEEEEECCHHHCCC
PIEHPLASQGDELDGMTVFDNVFIPWSHVFHLGNPEHAKLYPQRVFDWLHYHALIRQSVR
CCCCCCCCCCCCCCCEEEECCEECCHHHEEECCCCCHHHCCHHHHHHHHHHHHHHHHHHH
AELMAGLAILITEHIGTNKIPAVQTRVAKLIGFHQAMLAHIVASEELGFHTPGGAYKPNI
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCE
LIYDFGRALYLENFSQMIYELVDLSGRSALIFASEDQWNDEALNGWFERMNNGPVGQPHD
EEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHH
RVKIGRVIRDLFLTDWGNRLFVFENFNGTPLQAIRMLTMQRAEFSAAGPYGTLARKVCGI
HHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
ELTEGHDSEYKATAGYAQALDSARHQEKLALSGTMTV
EEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA