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The map label for this gene is rutF [H]

Identifier: 73541274

GI number: 73541274

Start: 1707275

End: 1707835

Strand: Direct

Name: rutF [H]

Synonym: Reut_A1584

Alternate gene names: 73541274

Gene position: 1707275-1707835 (Clockwise)

Preceding gene: 73541270

Following gene: 73541275

Centisome position: 44.85

GC content: 68.27

Gene sequence:

>561_bases
ATGTCGTCCGCAGTCTTCATCGAAGCCGGCGCGAGCCGCATTACCGACGCCGCCATCGACGCCATCGACGCCACGCGCTT
CCGCGACGCCTTGTCGCGTGCAGTCACCGCCGTGACCGTCATCGCCACCGACGGCGCCGGAGGCCGCGCTGGCGTCACGT
GCTCGGCTGTTTGTTCCGTGAGCGATTCGCCGCCCACCGTGCTGTTCTGCATCAATCGCCGCAGCGCCGCAAACAGCGTG
ATCAAGTCCAACGGCGTCGTCAGCATCAACTGGCTGCACGCCAGCCAGACCGCGGTGTCCCAGCTGTTCGCCGGTGCCGG
CGCGGTGCCGATGGCGCAGCGCTTCGACGGTGACAGCTGGGGTGTGTTCGGCACCGGCGCGCCATACAGCAAGTCCGCCA
TGGTCGCGCTCGACTGCAAGGTCGTTGACCAGCTCGAGATCGGCACGCACAGCGTCTTCGTCGCGCAGGTGCTCGGCAGC
TCGGGCGCGGAAAGCAGCAACGACACGCCGCTGGTCTACTGCCGCCGCAGCTACGCCACCACGCAGCCAGTCGCCGCCTA
G

Upstream 100 bases:

>100_bases
ACAGCTGACACAGATCAGAAAGCAGGTTTCCAGAGACAAAGCTTCATTCAAGCCCTGTCGCAGGGCAACGAAGAAGGCCC
CCAACAAGGAGGAGATCGCC

Downstream 100 bases:

>100_bases
CGCGACTTGAAGCGGCCTGGCCCGCGCCAGGCGAGAACCCGATTTCGTATCCGTGTCCATATGCCCTTCTCCAGGGCAGG
GACTGTCACACCCCGAAAAC

Product: flavin reductase-like, FMN-binding

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 186; Mature: 185

Protein sequence:

>186_residues
MSSAVFIEAGASRITDAAIDAIDATRFRDALSRAVTAVTVIATDGAGGRAGVTCSAVCSVSDSPPTVLFCINRRSAANSV
IKSNGVVSINWLHASQTAVSQLFAGAGAVPMAQRFDGDSWGVFGTGAPYSKSAMVALDCKVVDQLEIGTHSVFVAQVLGS
SGAESSNDTPLVYCRRSYATTQPVAA

Sequences:

>Translated_186_residues
MSSAVFIEAGASRITDAAIDAIDATRFRDALSRAVTAVTVIATDGAGGRAGVTCSAVCSVSDSPPTVLFCINRRSAANSV
IKSNGVVSINWLHASQTAVSQLFAGAGAVPMAQRFDGDSWGVFGTGAPYSKSAMVALDCKVVDQLEIGTHSVFVAQVLGS
SGAESSNDTPLVYCRRSYATTQPVAA
>Mature_185_residues
SSAVFIEAGASRITDAAIDAIDATRFRDALSRAVTAVTVIATDGAGGRAGVTCSAVCSVSDSPPTVLFCINRRSAANSVI
KSNGVVSINWLHASQTAVSQLFAGAGAVPMAQRFDGDSWGVFGTGAPYSKSAMVALDCKVVDQLEIGTHSVFVAQVLGSS
GAESSNDTPLVYCRRSYATTQPVAA

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=162, Percent_Identity=38.8888888888889, Blast_Score=114, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 19025; Mature: 18894

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSAVFIEAGASRITDAAIDAIDATRFRDALSRAVTAVTVIATDGAGGRAGVTCSAVCSV
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCEEEEEEEEC
SDSPPTVLFCINRRSAANSVIKSNGVVSINWLHASQTAVSQLFAGAGAVPMAQRFDGDSW
CCCCCEEEEEECCCCHHHHHHHCCCEEEEEEEECCHHHHHHHHHCCCCCCHHHHCCCCCC
GVFGTGAPYSKSAMVALDCKVVDQLEIGTHSVFVAQVLGSSGAESSNDTPLVYCRRSYAT
CEEECCCCCCCCEEEEEEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCCC
TQPVAA
CCCCCC
>Mature Secondary Structure 
SSAVFIEAGASRITDAAIDAIDATRFRDALSRAVTAVTVIATDGAGGRAGVTCSAVCSV
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCEEEEEEEEC
SDSPPTVLFCINRRSAANSVIKSNGVVSINWLHASQTAVSQLFAGAGAVPMAQRFDGDSW
CCCCCEEEEEECCCCHHHHHHHCCCEEEEEEEECCHHHHHHHHHCCCCCCHHHHCCCCCC
GVFGTGAPYSKSAMVALDCKVVDQLEIGTHSVFVAQVLGSSGAESSNDTPLVYCRRSYAT
CEEECCCCCCCCEEEEEEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCCC
TQPVAA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA