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The map label for this gene is lip2 [H]

Identifier: 73541227

GI number: 73541227

Start: 1656105

End: 1657061

Strand: Reverse

Name: lip2 [H]

Synonym: Reut_A1537

Alternate gene names: 73541227

Gene position: 1657061-1656105 (Counterclockwise)

Preceding gene: 73541228

Following gene: 73541226

Centisome position: 43.53

GC content: 71.58

Gene sequence:

>957_bases
ATGCCGCTCGATCCCCAGGCCCGCGCCATGCTGGACGCCATGGCCGCCATGCCGAGTCCCGACTTCACCACGCTGCGCGC
TGCCGACTACCGTGCGGCAGTCGCCGCCATGCCGGCCTTTGCGCCCGGCGACAGCGTTGCCGCGCAGCAAGACCTGACCA
TCGATGGCGCCGCGGGCCCGTTGCCGGCGCGCCTGTACCGGCCCGACGACAGCGCAGGTCCGCCGCTCATCGTTTATTTC
CATGGCGGCGGCTTCGTGCTCTGCGGCCTCGATTCGCACGACAACATCTGCCGCAGCCTGGCGCGTCGCAGCGGCGCGCT
GGTGCTGTCGGTCGACTACCGGCTGGCTCCGGAAGCGCGCTTCCCGGCCGCGGCCGACGACGCGGTTGCGGCTGTGCGCT
GGGCCGCCGGGCACGCCGCCGAGCTTGGCGTCGACCCGGCCCGGCTGGCAGTGGCCGGCGACAGCGCCGGCGGCAACCTC
GCCGCAGTCGTGTGCCAGCAACTGCGCGGCAGCGGCATCGCGCTGAAGCACCAGTTGCTGCTCTACCCTTACCTGGACTG
CACTGACGCCGCCGCCGACAGCGCCAGTTACCGCGCCTGTGCCGAAGGCTATTTCCTCAGTGCGCGCGAACTCGACTGGT
GTCGCGCCCAGTACCTGGCCAATCCGGACGCCGCCGCCGATGTCCGCGCGAGCCCCTTGCACCAGCGAGATCTGCAGGGC
CTGCCGGCCGCCACCATCATCACGGCGGAATTCGATCCGCTGCGCGACCAGGGCGAAGCGTATGGCGAAGCGTTGCGGGG
CGCGGGCGGTGCGGCCACCGTACGCCGCTGGCCGGGCCAGTTCCATGGCTTCATGAGCATGCAGGGCGTGCTCGATGCCG
CGAGCCATGCGCTCGATGCCGCCGCTGCGTCATTGCGCCAGGCTTTCGCCGCCAGCGGCACGCAGGAGGCAGCATGA

Upstream 100 bases:

>100_bases
CCGCCCAAGCCGCGCTCGTACGCGCGCAAGGCATTGCCGCCGGCAAGGCGCCCGCCTTCGCGCTCTGACCATCATCACCC
CAAGTTACTCAGGAGACCCG

Downstream 100 bases:

>100_bases
ACGCCGCCATGCTCCCGCCGATGCTGGACGTGCTCGCGGCGCTGGCTGCGGGACAGCGTGTGGTGGTGCTCGAAGACGAG
ACCGACGAAGCCCTTGGCTG

Product: lipolytic protein

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MPLDPQARAMLDAMAAMPSPDFTTLRAADYRAAVAAMPAFAPGDSVAAQQDLTIDGAAGPLPARLYRPDDSAGPPLIVYF
HGGGFVLCGLDSHDNICRSLARRSGALVLSVDYRLAPEARFPAAADDAVAAVRWAAGHAAELGVDPARLAVAGDSAGGNL
AAVVCQQLRGSGIALKHQLLLYPYLDCTDAAADSASYRACAEGYFLSARELDWCRAQYLANPDAAADVRASPLHQRDLQG
LPAATIITAEFDPLRDQGEAYGEALRGAGGAATVRRWPGQFHGFMSMQGVLDAASHALDAAAASLRQAFAASGTQEAA

Sequences:

>Translated_318_residues
MPLDPQARAMLDAMAAMPSPDFTTLRAADYRAAVAAMPAFAPGDSVAAQQDLTIDGAAGPLPARLYRPDDSAGPPLIVYF
HGGGFVLCGLDSHDNICRSLARRSGALVLSVDYRLAPEARFPAAADDAVAAVRWAAGHAAELGVDPARLAVAGDSAGGNL
AAVVCQQLRGSGIALKHQLLLYPYLDCTDAAADSASYRACAEGYFLSARELDWCRAQYLANPDAAADVRASPLHQRDLQG
LPAATIITAEFDPLRDQGEAYGEALRGAGGAATVRRWPGQFHGFMSMQGVLDAASHALDAAAASLRQAFAASGTQEAA
>Mature_317_residues
PLDPQARAMLDAMAAMPSPDFTTLRAADYRAAVAAMPAFAPGDSVAAQQDLTIDGAAGPLPARLYRPDDSAGPPLIVYFH
GGGFVLCGLDSHDNICRSLARRSGALVLSVDYRLAPEARFPAAADDAVAAVRWAAGHAAELGVDPARLAVAGDSAGGNLA
AVVCQQLRGSGIALKHQLLLYPYLDCTDAAADSASYRACAEGYFLSARELDWCRAQYLANPDAAADVRASPLHQRDLQGL
PAATIITAEFDPLRDQGEAYGEALRGAGGAATVRRWPGQFHGFMSMQGVLDAASHALDAAAASLRQAFAASGTQEAA

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=285, Percent_Identity=32.280701754386, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI206597554, Length=329, Percent_Identity=29.1793313069909, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI68051721, Length=275, Percent_Identity=28.3636363636364, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI157041239, Length=183, Percent_Identity=35.5191256830601, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI226423947, Length=283, Percent_Identity=27.5618374558304, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI21328446, Length=89, Percent_Identity=50.561797752809, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI61966717, Length=170, Percent_Identity=31.1764705882353, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI157041237, Length=112, Percent_Identity=38.3928571428571, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI1786682, Length=246, Percent_Identity=32.9268292682927, Blast_Score=108, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17567059, Length=272, Percent_Identity=31.6176470588235, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17540028, Length=170, Percent_Identity=34.1176470588235, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71996133, Length=143, Percent_Identity=34.2657342657343, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI72001146, Length=124, Percent_Identity=35.4838709677419, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI115533412, Length=125, Percent_Identity=32, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI115533410, Length=125, Percent_Identity=32, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24656084, Length=109, Percent_Identity=37.6146788990826, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24656076, Length=109, Percent_Identity=37.6146788990826, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI20130169, Length=109, Percent_Identity=37.6146788990826, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 33035; Mature: 32904

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLDPQARAMLDAMAAMPSPDFTTLRAADYRAAVAAMPAFAPGDSVAAQQDLTIDGAAGP
CCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCC
LPARLYRPDDSAGPPLIVYFHGGGFVLCGLDSHDNICRSLARRSGALVLSVDYRLAPEAR
CCCEEECCCCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHCCCCEEEEECCEECCCCC
FPAAADDAVAAVRWAAGHAAELGVDPARLAVAGDSAGGNLAAVVCQQLRGSGIALKHQLL
CCCCCCHHHHHHHHHCCCHHHCCCCHHHEEEECCCCCCHHHHHHHHHHCCCCEEEEEEEE
LYPYLDCTDAAADSASYRACAEGYFLSARELDWCRAQYLANPDAAADVRASPLHQRDLQG
EEECCCCCHHHCCCCHHHHHHCCCEECCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCC
LPAATIITAEFDPLRDQGEAYGEALRGAGGAATVRRWPGQFHGFMSMQGVLDAASHALDA
CCCEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AAASLRQAFAASGTQEAA
HHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PLDPQARAMLDAMAAMPSPDFTTLRAADYRAAVAAMPAFAPGDSVAAQQDLTIDGAAGP
CCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCC
LPARLYRPDDSAGPPLIVYFHGGGFVLCGLDSHDNICRSLARRSGALVLSVDYRLAPEAR
CCCEEECCCCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHCCCCEEEEECCEECCCCC
FPAAADDAVAAVRWAAGHAAELGVDPARLAVAGDSAGGNLAAVVCQQLRGSGIALKHQLL
CCCCCCHHHHHHHHHCCCHHHCCCCHHHEEEECCCCCCHHHHHHHHHHCCCCEEEEEEEE
LYPYLDCTDAAADSASYRACAEGYFLSARELDWCRAQYLANPDAAADVRASPLHQRDLQG
EEECCCCCHHHCCCCHHHHHHCCCEECCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCC
LPAATIITAEFDPLRDQGEAYGEALRGAGGAATVRRWPGQFHGFMSMQGVLDAASHALDA
CCCEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AAASLRQAFAASGTQEAA
HHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]