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The map label for this gene is paaF [C]

Identifier: 73541158

GI number: 73541158

Start: 1583738

End: 1584526

Strand: Reverse

Name: paaF [C]

Synonym: Reut_A1464

Alternate gene names: 73541158

Gene position: 1584526-1583738 (Counterclockwise)

Preceding gene: 73541159

Following gene: 73541157

Centisome position: 41.63

GC content: 67.55

Gene sequence:

>789_bases
ATGGAATTCACCACGCTTACCGTCACCATCGCCCGGCATGTCGCGACCGTGACGCTGAACCGCCCCGACGTGCGCAATGC
GTTCAACGAAACCGTCATCGCCGAACTGACCGGCGCCTTCCGCGCGCTCGGTGACATGGACGAAGTGCGCGCGATCGTGC
TCGCCGGCAACGGCCCCGCGTTCTGCGCAGGCGCCGACCTGAACTGGATGAAGAAGATGGCCGGTTACTCGAACGACGAG
AACCGTGCCGATGCTCTGGCGCTCGCGCAGATGCTGCACACCATCTGGTCCTGCCCGCGCCCGGTCATTGCCCGCATCCA
GGGCGACACCTATGCCGGCGGCGTCGGCCTTGCCGCGGCGTGCGACATCGCGGTGGCCGCCGACCACGCCCACTTCTGCC
TGTCCGAAGCGCGCCTGGGCCTGCTGCCCGCGACCATCAGCCCGTACGTGATCCGCGCCATGGGTGAGCAGGCATCGCGC
CGCTACTTCATTACGGCCGAGCGTTTCGACGCCGCGGAAGCGTTGCGCCTCGGCTTCGTCCACGCCGTAGTACCGGCCGA
TGCGCTCGACGCCAAGGTGACGGAAATCGCTTCGGCACTGGTCGGCAACAGTCCCAACGCCGTGCGCGAGAGCAAACGAC
TTGTGCAGGAAGTGGCGGGCAGACCGGTCGACAACAACCTGCTGGCCGATACGGCGGACCGCATCGCCGCGATCCGCGCC
TCGGACGAAGGCCGCGAAGGCGTGCGCGCGTTCCTGGAAAAGCGCTCGCCTGCCTGGAAGCCCGCGTAA

Upstream 100 bases:

>100_bases
GCCTGTCGGCCAGCCTCAACGCGCCGATCGACGACATGAAGTTCGGCGTGTTCCGCATGTAAGCCGGCCCCCGACCAACG
CACATTCAAGCCATACCGCC

Downstream 100 bases:

>100_bases
GCGCTTCCGACACCCCTACAAAACGAACCCCGGCAGCATGCCGAGGCGGCGTCCGCCTGCTGCCCGCATGCGTCAGCGGC
CGGCGTGATGCCGCTGCCCT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MEFTTLTVTIARHVATVTLNRPDVRNAFNETVIAELTGAFRALGDMDEVRAIVLAGNGPAFCAGADLNWMKKMAGYSNDE
NRADALALAQMLHTIWSCPRPVIARIQGDTYAGGVGLAAACDIAVAADHAHFCLSEARLGLLPATISPYVIRAMGEQASR
RYFITAERFDAAEALRLGFVHAVVPADALDAKVTEIASALVGNSPNAVRESKRLVQEVAGRPVDNNLLADTADRIAAIRA
SDEGREGVRAFLEKRSPAWKPA

Sequences:

>Translated_262_residues
MEFTTLTVTIARHVATVTLNRPDVRNAFNETVIAELTGAFRALGDMDEVRAIVLAGNGPAFCAGADLNWMKKMAGYSNDE
NRADALALAQMLHTIWSCPRPVIARIQGDTYAGGVGLAAACDIAVAADHAHFCLSEARLGLLPATISPYVIRAMGEQASR
RYFITAERFDAAEALRLGFVHAVVPADALDAKVTEIASALVGNSPNAVRESKRLVQEVAGRPVDNNLLADTADRIAAIRA
SDEGREGVRAFLEKRSPAWKPA
>Mature_262_residues
MEFTTLTVTIARHVATVTLNRPDVRNAFNETVIAELTGAFRALGDMDEVRAIVLAGNGPAFCAGADLNWMKKMAGYSNDE
NRADALALAQMLHTIWSCPRPVIARIQGDTYAGGVGLAAACDIAVAADHAHFCLSEARLGLLPATISPYVIRAMGEQASR
RYFITAERFDAAEALRLGFVHAVVPADALDAKVTEIASALVGNSPNAVRESKRLVQEVAGRPVDNNLLADTADRIAAIRA
SDEGREGVRAFLEKRSPAWKPA

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=256, Percent_Identity=29.296875, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI31542718, Length=246, Percent_Identity=26.8292682926829, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI70995211, Length=272, Percent_Identity=32.3529411764706, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI45643119, Length=256, Percent_Identity=27.34375, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI260275230, Length=256, Percent_Identity=27.34375, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI260274832, Length=256, Percent_Identity=27.34375, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI4502327, Length=257, Percent_Identity=31.1284046692607, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI68989263, Length=218, Percent_Identity=28.4403669724771, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1787659, Length=264, Percent_Identity=31.4393939393939, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1788597, Length=246, Percent_Identity=30.8943089430894, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI221142681, Length=269, Percent_Identity=29.368029739777, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1787660, Length=261, Percent_Identity=28.3524904214559, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87082183, Length=277, Percent_Identity=24.9097472924188, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788682, Length=221, Percent_Identity=31.6742081447964, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=30.4, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17554946, Length=254, Percent_Identity=29.9212598425197, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17540306, Length=187, Percent_Identity=28.8770053475936, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17540714, Length=201, Percent_Identity=31.3432835820896, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17536985, Length=230, Percent_Identity=28.2608695652174, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17534483, Length=236, Percent_Identity=24.5762711864407, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17549921, Length=202, Percent_Identity=26.7326732673267, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=257, Percent_Identity=26.0700389105058, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI20129971, Length=250, Percent_Identity=30.4, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24653477, Length=250, Percent_Identity=30.4, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24653139, Length=266, Percent_Identity=29.6992481203008, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19922422, Length=264, Percent_Identity=25.7575757575758, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19920382, Length=225, Percent_Identity=29.3333333333333, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24654903, Length=222, Percent_Identity=22.5225225225225, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28040; Mature: 28040

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFTTLTVTIARHVATVTLNRPDVRNAFNETVIAELTGAFRALGDMDEVRAIVLAGNGPA
CCEEEEEHEEEHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEEECCCCC
FCAGADLNWMKKMAGYSNDENRADALALAQMLHTIWSCPRPVIARIQGDTYAGGVGLAAA
EEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHEEECCCCCCCCCCHHHH
CDIAVAADHAHFCLSEARLGLLPATISPYVIRAMGEQASRRYFITAERFDAAEALRLGFV
HHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCEEEEEHHHHCHHHHHHHHHH
HAVVPADALDAKVTEIASALVGNSPNAVRESKRLVQEVAGRPVDNNLLADTADRIAAIRA
HHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHEEEEC
SDEGREGVRAFLEKRSPAWKPA
CCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MEFTTLTVTIARHVATVTLNRPDVRNAFNETVIAELTGAFRALGDMDEVRAIVLAGNGPA
CCEEEEEHEEEHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEEECCCCC
FCAGADLNWMKKMAGYSNDENRADALALAQMLHTIWSCPRPVIARIQGDTYAGGVGLAAA
EEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHEEECCCCCCCCCCHHHH
CDIAVAADHAHFCLSEARLGLLPATISPYVIRAMGEQASRRYFITAERFDAAEALRLGFV
HHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCEEEEEHHHHCHHHHHHHHHH
HAVVPADALDAKVTEIASALVGNSPNAVRESKRLVQEVAGRPVDNNLLADTADRIAAIRA
HHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHEEEEC
SDEGREGVRAFLEKRSPAWKPA
CCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA