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The map label for this gene is lip2 [H]

Identifier: 73541113

GI number: 73541113

Start: 1535682

End: 1536833

Strand: Direct

Name: lip2 [H]

Synonym: Reut_A1419

Alternate gene names: 73541113

Gene position: 1535682-1536833 (Clockwise)

Preceding gene: 73541110

Following gene: 73541114

Centisome position: 40.34

GC content: 66.32

Gene sequence:

>1152_bases
ATGATACTGAAATCGAACGACCGTTCACAATTCGAACTTCCCCCGACTTCGGGGGAAGCCCCGAGTCATAGTGCGGATGC
CCAACAGGCCGCCGCCCATGCTGTATCGACGCCAGTATCTGCCATGTCTGCCGTAACCTCCGCCTCGTCCGCTGTGGCGG
CCACCCAGTCTGTCGCTGGCATGCCTGCCGTGCCGCTCGACCCGCAGGTAGCGGCGCTGCTCGACCTGATTGCACGCGCC
AAGCGGCCACCCATTCATGTGATGGACGTGGAAGACGCCAAGATCGCCTACGAGAAGAGTGCGCCCATCCTTGATATCTC
GCCGCTGCCCATGCATGCGGTCGACGATCTCCACGTCTGCGCGCGCGACGGCCATGGGATTCCGGTACGCCTGTACGCGC
CGCGCGAGGCCAGTTGGGCCGATCCGCTGCCGATGCTCGTGTACTTTCACGGCGGCGGATTCACAGTCGGAAGCGTCAAT
TCCCACGACGCGCTGTGCCGCATGCAGGCTGCGCATGCCGACTGCATGGTGCTGTCGGTCGACTACCGCCTCGGACCGCA
ATGGCGCTTCCCGACCGCGGCCAACGACGCTTTCGACGTGCTGCACTGGGTCTTCGAAGAGGCCGCCCGGCTCGGCGCGG
ACCCCGCGCGCATTGCGCTGGGCGGTGACAGTGCCGGCGGCACGCTTGCCACGGCATGCGCCGTGCATGCGCGCGACAGC
GGGCTGGCGCCAGTGCTGCAGTTGCTGATCTATCCCGGCACCTGCGCGCGGCAGGACACGCCGTCGCACGGGGCATTGGC
GGAGGGCTATCTGCTCACGGCGGACATGATCCAGTGGTTCTTCTCGCATTACCTCGATCAGGAATCGAGCCGCGACGACT
GGCGCTTTGCTCCGCTCGATGGCGGCGGCAGTGGTGCAGATGTGCGCGGCTGCTGTCCGGCGTGGATTGCCGTGGCCGGC
TATGACCCGCTGCATGACGAAGGTGTGGCCTACGCCGATAAATTGCGCGCGGCGGGCGTGACGGTCACGCTGGCCGACTA
TGCTGGCATGATCCACGACTTTTTCAAGATGGGCCGCTTCGTGCCCGCCGTGGCGCAGGCGCATGCCGATGCCGTGGCGG
CGCTCCGGGCCGCTTTCGGCACCGGGCAGTAA

Upstream 100 bases:

>100_bases
CTGGCCAAGCGGATGAACTAGGGCGATCATCGCCCAAAAACGCCCACTCGCGTGCACGCATCGGGGTTATTTCGTAATGT
CTGCTTCTTGAAACACTGGC

Downstream 100 bases:

>100_bases
CGCCGGGCCGCGGCACGAATCACACGAATTGCGGTCCGAACTCAAAAACTTTCAGCAGTGACTTGCAGCACAAGGAGACA
TCGTCAAATGCAACGCCGCC

Product: lipolytic protein

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MILKSNDRSQFELPPTSGEAPSHSADAQQAAAHAVSTPVSAMSAVTSASSAVAATQSVAGMPAVPLDPQVAALLDLIARA
KRPPIHVMDVEDAKIAYEKSAPILDISPLPMHAVDDLHVCARDGHGIPVRLYAPREASWADPLPMLVYFHGGGFTVGSVN
SHDALCRMQAAHADCMVLSVDYRLGPQWRFPTAANDAFDVLHWVFEEAARLGADPARIALGGDSAGGTLATACAVHARDS
GLAPVLQLLIYPGTCARQDTPSHGALAEGYLLTADMIQWFFSHYLDQESSRDDWRFAPLDGGGSGADVRGCCPAWIAVAG
YDPLHDEGVAYADKLRAAGVTVTLADYAGMIHDFFKMGRFVPAVAQAHADAVAALRAAFGTGQ

Sequences:

>Translated_383_residues
MILKSNDRSQFELPPTSGEAPSHSADAQQAAAHAVSTPVSAMSAVTSASSAVAATQSVAGMPAVPLDPQVAALLDLIARA
KRPPIHVMDVEDAKIAYEKSAPILDISPLPMHAVDDLHVCARDGHGIPVRLYAPREASWADPLPMLVYFHGGGFTVGSVN
SHDALCRMQAAHADCMVLSVDYRLGPQWRFPTAANDAFDVLHWVFEEAARLGADPARIALGGDSAGGTLATACAVHARDS
GLAPVLQLLIYPGTCARQDTPSHGALAEGYLLTADMIQWFFSHYLDQESSRDDWRFAPLDGGGSGADVRGCCPAWIAVAG
YDPLHDEGVAYADKLRAAGVTVTLADYAGMIHDFFKMGRFVPAVAQAHADAVAALRAAFGTGQ
>Mature_383_residues
MILKSNDRSQFELPPTSGEAPSHSADAQQAAAHAVSTPVSAMSAVTSASSAVAATQSVAGMPAVPLDPQVAALLDLIARA
KRPPIHVMDVEDAKIAYEKSAPILDISPLPMHAVDDLHVCARDGHGIPVRLYAPREASWADPLPMLVYFHGGGFTVGSVN
SHDALCRMQAAHADCMVLSVDYRLGPQWRFPTAANDAFDVLHWVFEEAARLGADPARIALGGDSAGGTLATACAVHARDS
GLAPVLQLLIYPGTCARQDTPSHGALAEGYLLTADMIQWFFSHYLDQESSRDDWRFAPLDGGGSGADVRGCCPAWIAVAG
YDPLHDEGVAYADKLRAAGVTVTLADYAGMIHDFFKMGRFVPAVAQAHADAVAALRAAFGTGQ

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=289, Percent_Identity=28.0276816608997, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI206597554, Length=274, Percent_Identity=29.1970802919708, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI157041239, Length=185, Percent_Identity=33.5135135135135, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI68051721, Length=303, Percent_Identity=26.0726072607261, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI226423947, Length=310, Percent_Identity=25.1612903225806, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI21328446, Length=105, Percent_Identity=37.1428571428571, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI68508967, Length=109, Percent_Identity=32.1100917431193, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI68508957, Length=109, Percent_Identity=32.1100917431193, Blast_Score=66, Evalue=6e-11,
Organism=Homo sapiens, GI68508965, Length=109, Percent_Identity=32.1100917431193, Blast_Score=66, Evalue=6e-11,
Organism=Homo sapiens, GI61966717, Length=194, Percent_Identity=28.3505154639175, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1786682, Length=262, Percent_Identity=28.6259541984733, Blast_Score=102, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI72001146, Length=131, Percent_Identity=38.1679389312977, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI71996133, Length=131, Percent_Identity=35.1145038167939, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17540028, Length=128, Percent_Identity=34.375, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24656084, Length=135, Percent_Identity=34.0740740740741, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24656076, Length=135, Percent_Identity=34.0740740740741, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI20130169, Length=135, Percent_Identity=34.0740740740741, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 40346; Mature: 40346

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS01173 LIPASE_GDXG_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILKSNDRSQFELPPTSGEAPSHSADAQQAAAHAVSTPVSAMSAVTSASSAVAATQSVAG
CEECCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCC
MPAVPLDPQVAALLDLIARAKRPPIHVMDVEDAKIAYEKSAPILDISPLPMHAVDDLHVC
CCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCCEEECCCCCCHHHCHHHHH
ARDGHGIPVRLYAPREASWADPLPMLVYFHGGGFTVGSVNSHDALCRMQAAHADCMVLSV
HCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCEEECCCCCCHHHHHHHHCCCCEEEEEE
DYRLGPQWRFPTAANDAFDVLHWVFEEAARLGADPARIALGGDSAGGTLATACAVHARDS
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHCCCC
GLAPVLQLLIYPGTCARQDTPSHGALAEGYLLTADMIQWFFSHYLDQESSRDDWRFAPLD
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCEEECCC
GGGSGADVRGCCPAWIAVAGYDPLHDEGVAYADKLRAAGVTVTLADYAGMIHDFFKMGRF
CCCCCCCCCCCCHHHHEECCCCCCCCCCCHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHH
VPAVAQAHADAVAALRAAFGTGQ
HHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MILKSNDRSQFELPPTSGEAPSHSADAQQAAAHAVSTPVSAMSAVTSASSAVAATQSVAG
CEECCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCC
MPAVPLDPQVAALLDLIARAKRPPIHVMDVEDAKIAYEKSAPILDISPLPMHAVDDLHVC
CCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCCEEECCCCCCHHHCHHHHH
ARDGHGIPVRLYAPREASWADPLPMLVYFHGGGFTVGSVNSHDALCRMQAAHADCMVLSV
HCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCEEECCCCCCHHHHHHHHCCCCEEEEEE
DYRLGPQWRFPTAANDAFDVLHWVFEEAARLGADPARIALGGDSAGGTLATACAVHARDS
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHCCCC
GLAPVLQLLIYPGTCARQDTPSHGALAEGYLLTADMIQWFFSHYLDQESSRDDWRFAPLD
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCEEECCC
GGGSGADVRGCCPAWIAVAGYDPLHDEGVAYADKLRAAGVTVTLADYAGMIHDFFKMGRF
CCCCCCCCCCCCHHHHEECCCCCCCCCCCHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHH
VPAVAQAHADAVAALRAAFGTGQ
HHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]