BacMap

Definition	Ralstonia eutropha JMP134 chromosome 1, complete sequence.
Accession	NC_007347
Length	3,806,533

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The map label for this gene is ligD [H]

Identifier: 73541109

GI number: 73541109

Start: 1531809

End: 1532711

Strand: Direct

Name: ligD [H]

Synonym: Reut_A1415

Alternate gene names: 73541109

Gene position: 1531809-1532711 (Clockwise)

Preceding gene: 73541108

Following gene: 73541110

Centisome position: 40.24

GC content: 65.45

Gene sequence:

>903_bases
ATGAATCAATTCGAAGGCCGAGTGGCCGTCATCACGGGCGGCGCGTCCGGTTTCGGCAAGGAGTTCGCACGCATCGGCGC
CGGGCTGGACATGAAGCTGGTGCTGGCCGATGTCCAGCAGGATGCGCTGGATGCAACCGTGGCCGAACTGAGGGGACAAG
GCGCGGATGTGATCGGCTTGCGCACTGATGTTTCGCGCGGCGAGCAGGTGCAGGCGCTGGCCGATGCGGCCATGGAGCGC
TTCGGGCAGGTCAACCTGCTCTTCAACAACGCGGGCGTTGGCGCGGGCGGCCTGTTGTGGGAGAACTCGCTGAAGGACTG
GGAGTGGGTCCTGGGCGTGAACCTGTATGGCGTGGTCCACGGCGTGCGCATCTTCACGCCGCTGATGCTGGCCGCGGCCG
CCAAGGATCCGACCTATCAGGCCCACATCGTCAACACGGCCTCGATGGCCGGCATGCTGAGCCCGCCGGCCATGGGCGTG
TACAACGTATCGAAGCATGCCGTGGTGACGCTGACCGAAACGCTCTACCAGGACCTGGGCCTCGTCACGGAGCAGGTGCG
TTGCTCGGTGCTGTGCCCGTACTTCGTGCCGACCGGCATCAACCAGTCGCAGCGCAACCGTCCCGCGGACCTGACCAACG
ATGCGCCGCCCACGCGTTCGCAGATGGTGTCGCAGGCGCTGACGGACAAGGCGGTCACGTCGGGCAAGGTCACGGCCGAG
GACGTGGCACAGAAGACCTTCGATGCGATTCGTGCGGAAAGCTTCTATATTTATTCGCACCCGCAGGCCCTCGCGCCCGT
GCAGCAACGTTTCGAGGACATCATCGGCCAGCGCAATCCCGGCGATCCGTTCGCGGACAAGCCCGAGGTACGCGCAGGAC
TGGTTGCCGCGTTGCGCGGCTGA

Upstream 100 bases:

>100_bases
TCGCATGGCGCTGAAGGCGCTGAAACAGGCAGGCCGCTGAGCCGGGCCAAAGAAGGTCCGATAAGGACCTGTACACGACA
TAGCGAGAGGGGACAAACAC

Downstream 100 bases:

>100_bases
TCGACGAACCAACCACACGAGGCCCGCGCATGGAGACCCCCATGACCGCTCCCGCCGTCATCCGTCATCTGCAGTTCGCC
AACCTGCCAAATGGCACGCG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MNQFEGRVAVITGGASGFGKEFARIGAGLDMKLVLADVQQDALDATVAELRGQGADVIGLRTDVSRGEQVQALADAAMER
FGQVNLLFNNAGVGAGGLLWENSLKDWEWVLGVNLYGVVHGVRIFTPLMLAAAAKDPTYQAHIVNTASMAGMLSPPAMGV
YNVSKHAVVTLTETLYQDLGLVTEQVRCSVLCPYFVPTGINQSQRNRPADLTNDAPPTRSQMVSQALTDKAVTSGKVTAE
DVAQKTFDAIRAESFYIYSHPQALAPVQQRFEDIIGQRNPGDPFADKPEVRAGLVAALRG

Sequences:

>Translated_300_residues
MNQFEGRVAVITGGASGFGKEFARIGAGLDMKLVLADVQQDALDATVAELRGQGADVIGLRTDVSRGEQVQALADAAMER
FGQVNLLFNNAGVGAGGLLWENSLKDWEWVLGVNLYGVVHGVRIFTPLMLAAAAKDPTYQAHIVNTASMAGMLSPPAMGV
YNVSKHAVVTLTETLYQDLGLVTEQVRCSVLCPYFVPTGINQSQRNRPADLTNDAPPTRSQMVSQALTDKAVTSGKVTAE
DVAQKTFDAIRAESFYIYSHPQALAPVQQRFEDIIGQRNPGDPFADKPEVRAGLVAALRG
>Mature_300_residues
MNQFEGRVAVITGGASGFGKEFARIGAGLDMKLVLADVQQDALDATVAELRGQGADVIGLRTDVSRGEQVQALADAAMER
FGQVNLLFNNAGVGAGGLLWENSLKDWEWVLGVNLYGVVHGVRIFTPLMLAAAAKDPTYQAHIVNTASMAGMLSPPAMGV
YNVSKHAVVTLTETLYQDLGLVTEQVRCSVLCPYFVPTGINQSQRNRPADLTNDAPPTRSQMVSQALTDKAVTSGKVTAE
DVAQKTFDAIRAESFYIYSHPQALAPVQQRFEDIIGQRNPGDPFADKPEVRAGLVAALRG

Specific function: Catalyzes the C alpha dehydrogenation of arylglycerol- beta-aryl ether (C alpha alcohol type) (compound IV) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI142976729, Length=196, Percent_Identity=33.6734693877551, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI31542939, Length=200, Percent_Identity=30, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI25282469, Length=256, Percent_Identity=28.125, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI40807363, Length=203, Percent_Identity=27.5862068965517, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI20149619, Length=202, Percent_Identity=28.7128712871287, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI33667109, Length=200, Percent_Identity=30.5, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI5031737, Length=197, Percent_Identity=30.4568527918782, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI210032110, Length=275, Percent_Identity=25.0909090909091, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI310128830, Length=262, Percent_Identity=25.5725190839695, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI310115468, Length=262, Percent_Identity=25.5725190839695, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI109715829, Length=202, Percent_Identity=26.7326732673267, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI224922801, Length=171, Percent_Identity=29.2397660818713, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI83715985, Length=181, Percent_Identity=29.8342541436464, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI66933014, Length=269, Percent_Identity=26.0223048327138, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI4758504, Length=181, Percent_Identity=29.8342541436464, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI87082100, Length=213, Percent_Identity=29.5774647887324, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787905, Length=127, Percent_Identity=30.7086614173228, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082160, Length=206, Percent_Identity=26.6990291262136, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789208, Length=223, Percent_Identity=26.457399103139, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787335, Length=201, Percent_Identity=22.8855721393035, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI193203117, Length=235, Percent_Identity=28.5106382978723, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI193203115, Length=232, Percent_Identity=28.8793103448276, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17564282, Length=202, Percent_Identity=28.7128712871287, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17508895, Length=196, Percent_Identity=26.530612244898, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71994600, Length=201, Percent_Identity=26.865671641791, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17561402, Length=202, Percent_Identity=25.7425742574257, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17567345, Length=233, Percent_Identity=29.1845493562232, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71994604, Length=200, Percent_Identity=25, Blast_Score=71, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17568967, Length=246, Percent_Identity=25.609756097561, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17555706, Length=216, Percent_Identity=25.462962962963, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI25147288, Length=214, Percent_Identity=27.1028037383178, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17538486, Length=218, Percent_Identity=28.4403669724771, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17560332, Length=213, Percent_Identity=26.7605633802817, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6323882, Length=199, Percent_Identity=25.6281407035176, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24581726, Length=209, Percent_Identity=30.622009569378, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355319, Length=205, Percent_Identity=29.2682926829268, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI18859837, Length=225, Percent_Identity=29.3333333333333, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI28571526, Length=203, Percent_Identity=28.0788177339901, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI281364892, Length=203, Percent_Identity=26.6009852216749, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24640492, Length=251, Percent_Identity=24.3027888446215, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24644339, Length=194, Percent_Identity=28.3505154639175, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21357041, Length=199, Percent_Identity=27.1356783919598, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17737361, Length=192, Percent_Identity=29.1666666666667, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24639444, Length=194, Percent_Identity=27.8350515463918, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI23397609, Length=201, Percent_Identity=27.363184079602, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI116007236, Length=209, Percent_Identity=29.6650717703349, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI281364494, Length=215, Percent_Identity=28.3720930232558, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI221510726, Length=195, Percent_Identity=28.2051282051282, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648944, Length=243, Percent_Identity=25.1028806584362, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21355631, Length=198, Percent_Identity=28.2828282828283, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI28571387, Length=207, Percent_Identity=27.536231884058, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 32118; Mature: 32118

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQFEGRVAVITGGASGFGKEFARIGAGLDMKLVLADVQQDALDATVAELRGQGADVIGL
CCCCCCEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
RTDVSRGEQVQALADAAMERFGQVNLLFNNAGVGAGGLLWENSLKDWEWVLGVNLYGVVH
ECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEECCCCCCEEEEECCHHHHHHH
GVRIFTPLMLAAAAKDPTYQAHIVNTASMAGMLSPPAMGVYNVSKHAVVTLTETLYQDLG
HHHHHHHHHHHHHCCCCCEEEEEEEHHHHHCCCCCCCCCEEECCCCEEEHHHHHHHHHHH
LVTEQVRCSVLCPYFVPTGINQSQRNRPADLTNDAPPTRSQMVSQALTDKAVTSGKVTAE
HHHHHHHHEEECCHHCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHH
DVAQKTFDAIRAESFYIYSHPQALAPVQQRFEDIIGQRNPGDPFADKPEVRAGLVAALRG
HHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNQFEGRVAVITGGASGFGKEFARIGAGLDMKLVLADVQQDALDATVAELRGQGADVIGL
CCCCCCEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
RTDVSRGEQVQALADAAMERFGQVNLLFNNAGVGAGGLLWENSLKDWEWVLGVNLYGVVH
ECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEECCCCCCEEEEECCHHHHHHH
GVRIFTPLMLAAAAKDPTYQAHIVNTASMAGMLSPPAMGVYNVSKHAVVTLTETLYQDLG
HHHHHHHHHHHHHCCCCCEEEEEEEHHHHHCCCCCCCCCEEECCCCEEEHHHHHHHHHHH
LVTEQVRCSVLCPYFVPTGINQSQRNRPADLTNDAPPTRSQMVSQALTDKAVTSGKVTAE
HHHHHHHHEEECCHHCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHH
DVAQKTFDAIRAESFYIYSHPQALAPVQQRFEDIIGQRNPGDPFADKPEVRAGLVAALRG
HHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1744051; 8495726; 7764263 [H]