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The map label for this gene is pyrC' [H]

Identifier: 73540415

GI number: 73540415

Start: 768434

End: 769780

Strand: Direct

Name: pyrC' [H]

Synonym: Reut_A0709

Alternate gene names: 73540415

Gene position: 768434-769780 (Clockwise)

Preceding gene: 73540414

Following gene: 73540416

Centisome position: 20.19

GC content: 66.3

Gene sequence:

>1347_bases
ATGCCCACTTCGAGCCAGAACATCCCGAACACGCCAAGCGACACGACAAGCCAAGCTATGAAGATTCACATCCAGGGCGG
CCGCCTGATCGACCCGGCCGCGAACCTCGACGCCCAGCAGGACCTCTATATCGCCGCCGGCAAAATTGTCGGCGTGGGCA
GCGCACCGGCGGACTTCACCGCCAACAAGACCATCGACGCCCGCGGCCTGATCGTGTGCCCGGGCCTCGTCGACTTGTCG
GCCCGCCTGCGCGAGCCCGGCTACGAATACAAGGCCACGCTCGAATCCGAAGTGGCCGCCGCGGCAGCGGGTGGCGTGAC
CAGTCTGCTCTGCCCGCCTGATACGGACCCTGTGCTCGACGAGCCCGGCCTGGTCGAGATGCTGAAGTTCCGCGCGCGCA
CGCTGAACCAGACCCACGTCTACCCGCTGGGCGCGCTGACGCAGGGCCTGAAGGGCGAAGTCCTGACCGAGATGAACCAG
CTGACCGAGGCCGGCTGCGTGGGCTTCAGCCAGGCCGAGGCGCCGGTCCGAAACACGCAGGTGCTGCTGCGTGCGCTGCA
GTACGCGCAGACCTTCGGCTTCACCGTGTGGCTGCGTCCGGAAGATCCTTTCCTCGGCGGCGGCGTGGCCGCGAGCGGCG
CCGTGGCGTCGCGCCTGGGGTTGTCGGGCGTGTCCGTGATTGCCGAGACGGTGCGCCTGCATACCATCTTCGAGCTGATG
CGCGCCAGCGGTGCGCGCGTGCATCTTTGCCGGCTGTCGACGGCAGCAGGTCTCGAGCTGGTGCGTCAGGCCAAGCGTGA
AGGCCTCGCTGTGACGTGCGACGTCAACATCCACCATGTCTCGCTGACCGACATGGATATCGGCTACTTCAACTCGCAGA
TGCGTTTTTCACCGCCGCTGCGCAGTGCGCGCGACCGCGATGCCATCGTCGCCGCGCTGGCCGACGGCACCATCGACGCG
CTGTGCTCGGATCACACGCCGGTCGACGACGACGAAAAGCTGCTTCCGTTTGCCGAAGCAACGCCGGGCGCCATCGGCCT
GGAACTGCTACTGCCGCTGACGCTGCGCTGGGCCGCCGAACATAAGGTGCCGCTGAAGACCGCTCTGGCACGCATCACCT
CCGAACCCGCGCGCGTGGCCGGCCTGAAGGCCGGTACGCTGGCGTCAGGCGCTGTCGCGGACATCTGTGTGTTCGATCCG
AAGGAAGTCTGGAAGGTCGACCGCCAGTCCATCAAGAGCCAGGGCAAGAACTCGCCGTGGCTCGGCTACGAGATGGAAGG
AAGGGTGCGTATGACGCTGGTCGGTGGCCAGGTTGTCTACGGCAACAACAATCACAACCACCACTGA

Upstream 100 bases:

>100_bases
GACGGCCCGCAATCGGTGATCCTGAACCAGGTGACGTTCGGCATCGCCGTGCGCATGGCCGTGATGGGTATCGTGGCAGG
AAACAGTGACTGACCACGCG

Downstream 100 bases:

>100_bases
CGCGCATGGTCTGGCTGCGAAAGGCGGCGCTGGTTCTGCATATGGTGCGCGGGTTGCTGACCTGCGCGGTGCTGTTTCCG
TGGCTGGGCGCGAGTTCGCG

Product: dihydroorotase

Products: N-carbamoyl-L-aspartate

Alternate protein names: Aspartate carbamoyltransferase 44 kDa Non-catalytic chain [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MPTSSQNIPNTPSDTTSQAMKIHIQGGRLIDPAANLDAQQDLYIAAGKIVGVGSAPADFTANKTIDARGLIVCPGLVDLS
ARLREPGYEYKATLESEVAAAAAGGVTSLLCPPDTDPVLDEPGLVEMLKFRARTLNQTHVYPLGALTQGLKGEVLTEMNQ
LTEAGCVGFSQAEAPVRNTQVLLRALQYAQTFGFTVWLRPEDPFLGGGVAASGAVASRLGLSGVSVIAETVRLHTIFELM
RASGARVHLCRLSTAAGLELVRQAKREGLAVTCDVNIHHVSLTDMDIGYFNSQMRFSPPLRSARDRDAIVAALADGTIDA
LCSDHTPVDDDEKLLPFAEATPGAIGLELLLPLTLRWAAEHKVPLKTALARITSEPARVAGLKAGTLASGAVADICVFDP
KEVWKVDRQSIKSQGKNSPWLGYEMEGRVRMTLVGGQVVYGNNNHNHH

Sequences:

>Translated_448_residues
MPTSSQNIPNTPSDTTSQAMKIHIQGGRLIDPAANLDAQQDLYIAAGKIVGVGSAPADFTANKTIDARGLIVCPGLVDLS
ARLREPGYEYKATLESEVAAAAAGGVTSLLCPPDTDPVLDEPGLVEMLKFRARTLNQTHVYPLGALTQGLKGEVLTEMNQ
LTEAGCVGFSQAEAPVRNTQVLLRALQYAQTFGFTVWLRPEDPFLGGGVAASGAVASRLGLSGVSVIAETVRLHTIFELM
RASGARVHLCRLSTAAGLELVRQAKREGLAVTCDVNIHHVSLTDMDIGYFNSQMRFSPPLRSARDRDAIVAALADGTIDA
LCSDHTPVDDDEKLLPFAEATPGAIGLELLLPLTLRWAAEHKVPLKTALARITSEPARVAGLKAGTLASGAVADICVFDP
KEVWKVDRQSIKSQGKNSPWLGYEMEGRVRMTLVGGQVVYGNNNHNHH
>Mature_447_residues
PTSSQNIPNTPSDTTSQAMKIHIQGGRLIDPAANLDAQQDLYIAAGKIVGVGSAPADFTANKTIDARGLIVCPGLVDLSA
RLREPGYEYKATLESEVAAAAAGGVTSLLCPPDTDPVLDEPGLVEMLKFRARTLNQTHVYPLGALTQGLKGEVLTEMNQL
TEAGCVGFSQAEAPVRNTQVLLRALQYAQTFGFTVWLRPEDPFLGGGVAASGAVASRLGLSGVSVIAETVRLHTIFELMR
ASGARVHLCRLSTAAGLELVRQAKREGLAVTCDVNIHHVSLTDMDIGYFNSQMRFSPPLRSARDRDAIVAALADGTIDAL
CSDHTPVDDDEKLLPFAEATPGAIGLELLLPLTLRWAAEHKVPLKTALARITSEPARVAGLKAGTLASGAVADICVFDPK
EVWKVDRQSIKSQGKNSPWLGYEMEGRVRMTLVGGQVVYGNNNHNHH

Specific function: Non-functional DHOase [H]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. PyrC' subfamily [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=195, Percent_Identity=29.7435897435897, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI19923821, Length=458, Percent_Identity=23.1441048034935, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1786722, Length=421, Percent_Identity=26.603325415677, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI87082175, Length=453, Percent_Identity=24.9448123620309, Blast_Score=96, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI193204318, Length=210, Percent_Identity=27.6190476190476, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322218, Length=394, Percent_Identity=26.3959390862944, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24642586, Length=400, Percent_Identity=25, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI18859883, Length=208, Percent_Identity=29.8076923076923, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR004722
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.5.2.3

Molecular weight: Translated: 47783; Mature: 47652

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTSSQNIPNTPSDTTSQAMKIHIQGGRLIDPAANLDAQQDLYIAAGKIVGVGSAPADFT
CCCCCCCCCCCCCCCCCCEEEEEECCCEEECCCCCCCCCCCEEEEECCEEECCCCCCCCC
ANKTIDARGLIVCPGLVDLSARLREPGYEYKATLESEVAAAAAGGVTSLLCPPDTDPVLD
CCCEECCCCEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
EPGLVEMLKFRARTLNQTHVYPLGALTQGLKGEVLTEMNQLTEAGCVGFSQAEAPVRNTQ
CCCHHHHHHHHHHHCCCCEEEEHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHH
VLLRALQYAQTFGFTVWLRPEDPFLGGGVAASGAVASRLGLSGVSVIAETVRLHTIFELM
HHHHHHHHHHHHCEEEEECCCCCCCCCCCHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHH
RASGARVHLCRLSTAAGLELVRQAKREGLAVTCDVNIHHVSLTDMDIGYFNSQMRFSPPL
HCCCCEEEEEECCHHHHHHHHHHHHHCCEEEEEEEEEEEEEEEEEECCCCCCCCEECCCC
RSARDRDAIVAALADGTIDALCSDHTPVDDDEKLLPFAEATPGAIGLELLLPLTLRWAAE
CCCCCCCCEEEEECCCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
HKVPLKTALARITSEPARVAGLKAGTLASGAVADICVFDPKEVWKVDRQSIKSQGKNSPW
CCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCCCC
LGYEMEGRVRMTLVGGQVVYGNNNHNHH
CCEEECCEEEEEEECCEEEECCCCCCCC
>Mature Secondary Structure 
PTSSQNIPNTPSDTTSQAMKIHIQGGRLIDPAANLDAQQDLYIAAGKIVGVGSAPADFT
CCCCCCCCCCCCCCCCCEEEEEECCCEEECCCCCCCCCCCEEEEECCEEECCCCCCCCC
ANKTIDARGLIVCPGLVDLSARLREPGYEYKATLESEVAAAAAGGVTSLLCPPDTDPVLD
CCCEECCCCEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
EPGLVEMLKFRARTLNQTHVYPLGALTQGLKGEVLTEMNQLTEAGCVGFSQAEAPVRNTQ
CCCHHHHHHHHHHHCCCCEEEEHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHH
VLLRALQYAQTFGFTVWLRPEDPFLGGGVAASGAVASRLGLSGVSVIAETVRLHTIFELM
HHHHHHHHHHHHCEEEEECCCCCCCCCCCHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHH
RASGARVHLCRLSTAAGLELVRQAKREGLAVTCDVNIHHVSLTDMDIGYFNSQMRFSPPL
HCCCCEEEEEECCHHHHHHHHHHHHHCCEEEEEEEEEEEEEEEEEECCCCCCCCEECCCC
RSARDRDAIVAALADGTIDALCSDHTPVDDDEKLLPFAEATPGAIGLELLLPLTLRWAAE
CCCCCCCCEEEEECCCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
HKVPLKTALARITSEPARVAGLKAGTLASGAVADICVFDPKEVWKVDRQSIKSQGKNSPW
CCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCCCC
LGYEMEGRVRMTLVGGQVVYGNNNHNHH
CCEEECCEEEEEEECCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-dihydroorotate; H2O

Specific reaction: (S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]