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The map label for this gene is pyrB [H]

Identifier: 73540414

GI number: 73540414

Start: 767455

End: 768426

Strand: Direct

Name: pyrB [H]

Synonym: Reut_A0708

Alternate gene names: 73540414

Gene position: 767455-768426 (Clockwise)

Preceding gene: 73540413

Following gene: 73540415

Centisome position: 20.16

GC content: 63.27

Gene sequence:

>972_bases
ATGACCAAGACGTTCCGCAACCCGCAGCTGACCAAGAACGGCGAACTGAAGCACCTGCTGTCGATCGAGGGATTGTCGCG
TGACATGATCACGCACATCCTCGATACCGCCAGCCAGTTCGTGTCGCTGTCCGACTCTGACCGCGATGTGAAGAAGGTGC
CGCTGCTGCGCGGCAAGAGCGTGTTCAACCTGTTCTTCGAGAACTCTACGCGTACGCGCACCACCTTCGAGATCGCCGCC
AAGCGGTTGTCGGCGGACGTGCTGAACCTGAATATCAACGCCTCGTCGACCAGCAAGGGCGAGTCGCTGCTGGACACGAT
CAACAACCTGTCGGCCATGTCGGCGGACATGTTCGTGGTGCGCCACGCCAGTTCGGGCGCGCCGTACCTGATCGCCGAAC
ACGTTGCACCGCACGTACACGTGATCAACGCCGGCGACGGTCGTCATGCGCACCCGACGCAAGGCCTGCTGGACATGTAC
ACGATCCGGCACTTCAAGAAGGACTTCACGCAACTGCGCGTGGCGATCGTTGGCGACATCCTGCACTCGCGCGTGGCGCG
TTCGGATATCCACGCGCTGACCACGCTCGGCGTGCCGGAAGTGCGCGCCATCGGCCCGCGTACGCTTCTGCCGTCGGGAC
TCGAGCAGATGGGCGTGCGTGTCTTCCACAACATGGAAGAGGGCCTGAAGGACGTGGATGTGGTGATCATGCTGCGCTTG
CAGAACGAACGCATGAGCGGCGCGCTACTGCCGTCGGCGCAGGAGTACTTCAAGGCCTTCGGCCTGACGCCGGAACGCCT
CGCGCTGGCCGCACCCGACGCGATCGTGATGCACCCGGGGCCGATGAACCGTGGCGTGGAGATTGACTCCGCGGTGGCGG
ACGGCCCGCAATCGGTGATCCTGAACCAGGTGACGTTCGGCATCGCCGTGCGCATGGCCGTGATGGGTATCGTGGCAGGA
AACAGTGACTGA

Upstream 100 bases:

>100_bases
TTTACCACCGAATCATCGGCCGGTTGATCCGGCCAATTTTTCGTTCACCGGCTCCGAGACCAGACTAGTCCCCCCGATTT
TTGCCCGCGCCTGACCACCC

Downstream 100 bases:

>100_bases
CCACGCGATGCCCACTTCGAGCCAGAACATCCCGAACACGCCAAGCGACACGACAAGCCAAGCTATGAAGATTCACATCC
AGGGCGGCCGCCTGATCGAC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MTKTFRNPQLTKNGELKHLLSIEGLSRDMITHILDTASQFVSLSDSDRDVKKVPLLRGKSVFNLFFENSTRTRTTFEIAA
KRLSADVLNLNINASSTSKGESLLDTINNLSAMSADMFVVRHASSGAPYLIAEHVAPHVHVINAGDGRHAHPTQGLLDMY
TIRHFKKDFTQLRVAIVGDILHSRVARSDIHALTTLGVPEVRAIGPRTLLPSGLEQMGVRVFHNMEEGLKDVDVVIMLRL
QNERMSGALLPSAQEYFKAFGLTPERLALAAPDAIVMHPGPMNRGVEIDSAVADGPQSVILNQVTFGIAVRMAVMGIVAG
NSD

Sequences:

>Translated_323_residues
MTKTFRNPQLTKNGELKHLLSIEGLSRDMITHILDTASQFVSLSDSDRDVKKVPLLRGKSVFNLFFENSTRTRTTFEIAA
KRLSADVLNLNINASSTSKGESLLDTINNLSAMSADMFVVRHASSGAPYLIAEHVAPHVHVINAGDGRHAHPTQGLLDMY
TIRHFKKDFTQLRVAIVGDILHSRVARSDIHALTTLGVPEVRAIGPRTLLPSGLEQMGVRVFHNMEEGLKDVDVVIMLRL
QNERMSGALLPSAQEYFKAFGLTPERLALAAPDAIVMHPGPMNRGVEIDSAVADGPQSVILNQVTFGIAVRMAVMGIVAG
NSD
>Mature_322_residues
TKTFRNPQLTKNGELKHLLSIEGLSRDMITHILDTASQFVSLSDSDRDVKKVPLLRGKSVFNLFFENSTRTRTTFEIAAK
RLSADVLNLNINASSTSKGESLLDTINNLSAMSADMFVVRHASSGAPYLIAEHVAPHVHVINAGDGRHAHPTQGLLDMYT
IRHFKKDFTQLRVAIVGDILHSRVARSDIHALTTLGVPEVRAIGPRTLLPSGLEQMGVRVFHNMEEGLKDVDVVIMLRLQ
NERMSGALLPSAQEYFKAFGLTPERLALAAPDAIVMHPGPMNRGVEIDSAVADGPQSVILNQVTFGIAVRMAVMGIVAGN
SD

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=314, Percent_Identity=35.3503184713376, Blast_Score=166, Evalue=3e-41,
Organism=Escherichia coli, GI2367364, Length=312, Percent_Identity=34.9358974358974, Blast_Score=141, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI193204318, Length=341, Percent_Identity=31.9648093841642, Blast_Score=116, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6322331, Length=315, Percent_Identity=31.4285714285714, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24642586, Length=317, Percent_Identity=34.0694006309148, Blast_Score=146, Evalue=2e-35,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 35247; Mature: 35116

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTFRNPQLTKNGELKHLLSIEGLSRDMITHILDTASQFVSLSDSDRDVKKVPLLRGKS
CCCCCCCCCCCCCCCCCEEHHHCCCCHHHHHHHHHHHHHHEECCCCCCCHHHCCCCCCCC
VFNLFFENSTRTRTTFEIAAKRLSADVLNLNINASSTSKGESLLDTINNLSAMSADMFVV
EEEEEECCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEE
RHASSGAPYLIAEHVAPHVHVINAGDGRHAHPTQGLLDMYTIRHFKKDFTQLRVAIVGDI
EECCCCCCEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHSRVARSDIHALTTLGVPEVRAIGPRTLLPSGLEQMGVRVFHNMEEGLKDVDVVIMLRL
HHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEE
QNERMSGALLPSAQEYFKAFGLTPERLALAAPDAIVMHPGPMNRGVEIDSAVADGPQSVI
CCCCCCCCCCCCHHHHHHHHCCCHHHHEEECCCEEEECCCCCCCCCEECHHHCCCHHHHH
LNQVTFGIAVRMAVMGIVAGNSD
HHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TKTFRNPQLTKNGELKHLLSIEGLSRDMITHILDTASQFVSLSDSDRDVKKVPLLRGKS
CCCCCCCCCCCCCCCCEEHHHCCCCHHHHHHHHHHHHHHEECCCCCCCHHHCCCCCCCC
VFNLFFENSTRTRTTFEIAAKRLSADVLNLNINASSTSKGESLLDTINNLSAMSADMFVV
EEEEEECCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEE
RHASSGAPYLIAEHVAPHVHVINAGDGRHAHPTQGLLDMYTIRHFKKDFTQLRVAIVGDI
EECCCCCCEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHSRVARSDIHALTTLGVPEVRAIGPRTLLPSGLEQMGVRVFHNMEEGLKDVDVVIMLRL
HHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEE
QNERMSGALLPSAQEYFKAFGLTPERLALAAPDAIVMHPGPMNRGVEIDSAVADGPQSVI
CCCCCCCCCCCCHHHHHHHHCCCHHHHEEECCCEEEECCCCCCCCCEECHHHCCCHHHHH
LNQVTFGIAVRMAVMGIVAGNSD
HHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA