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The map label for this gene is phrA [H]

Identifier: 73540409

GI number: 73540409

Start: 763449

End: 765026

Strand: Reverse

Name: phrA [H]

Synonym: Reut_A0703

Alternate gene names: 73540409

Gene position: 765026-763449 (Counterclockwise)

Preceding gene: 73540417

Following gene: 73540405

Centisome position: 20.1

GC content: 63.56

Gene sequence:

>1578_bases
ATGTTAGGGTATCGCCCGGCTAACCTTCACCAAGGCACCATGCAAGGCGCAGACACACCAGCCCACGCTCGCCCACCCTA
CCGCATCGACCACCAGTTCGACCGCGGCCTCGTGTGGTTCCGGCGCGACCTGAGGTCAACCGACCACGCGGCGCTGCATT
ATGCCCTCAAGCACTGCCGCGAGGTTTGGTGCGTATTCGTGTTCGACCGCGACATCCTGGACCCACTGCTAGCCCGCGGC
CTGAAAGGGGACCGGCGCGTCGATTTCATCCGCGCGTCGCTGATCGAACTCGATGCCACCTTGCGCGCCGCCGGCGGCGG
TCTGATCGTCATGAACGATTCGGCTGCAAGCGCGATTCCCGCCCTGGCCCGTGAATTGGGCGTGCAGGCCGTATTCGCCA
ACCATGACTATGAACCGGACGCCGTTGCCCGTGATGCTGCGGTCCGTGACTCGCTGGCCGGGGACGCGCGCGTGCTGTTC
ACGTTCAAGGATCAGGTCATCTTCGAGCGCGACGAGATCCTGACGAGCCAGGGCAAGCCGTTTGCCGTGTTCACGCCTTA
CAAGAACGCCTGGCTCAAGACAATCCAGCCATTTGACCTTCGGCCCTACCCGATCGAGCCATACCTGCATGCGCTGGCAC
CGCTTCCCTCGAAGCGGCTAGTTCCACTGCCCTCACTGGAGGATCTGGGATTTGCTCCGAGCAATCTCGCAGAGGTCGCC
ATGCCAACGGGCAGTTCAGGTGGCCAGGCATTGTTCGAGGAATTCACATCACGCATGGGCGACTACGGCCGCCGGCGTGA
CTACCCTGCCCTGCGCGGACCGAGCTATCTGTCCGTGCACCTGCGATTCGGCACGGTGTCGATCCGCACCCTGGCCCGCG
CCGCACATGGGGCAGTGCTGCGCGGCGGCGCGGACAGCGCTGGCGCCGCGACCTGGTTGTCGGAACTGGTCTGGCGGGAC
TTCTACTTCATGATCCTGCACCACCATCCGCGTGTGGCGGCTGGCGAGGCTTTCCATCCGGCCTACGACATCATCCGGTG
GCGCGACGGTGAAACCGGCGATCGCTATTTCGAGTCATGGCGCGATGCTGCTACCGGGTATCCGCTCGTCGATGCCGCGA
TGCTGCAGATCCGTCAAAGCGGTTATATGCACAACCGGCTTCGGATGGTGACGGCCAGCTTCCTGGTCAAAGACCTGGGC
GTGGACTGGCGCCGTGGCGAGCATTACTTTGCCGACCAACTCAACGACTTCGATTTCGCGGCCAACAACGGCGGCTGGCA
ATGGGCCGCCTCAACCGGATGCGATGCGCAGCCTTGGTTCCGCATTTTCAACCCGGTCACGCAATCGGAAAAATTCGACC
CGCAGGGCCGCTTTATCCGGAAGTACCTGCCACAACTCGCAGCATTGCCGGACAAGTACGTGCATGCACCGTGGACCGCA
CCGCAGGACGTGCTGCTCGCTGCAGGCGTTCGGCTGGGTGAGAACTATCCGCGCCCGCTCGTGCAGCACGACGTCGCGCG
CAAGGAAACACTGGAGCGCTATGCGGTGGTGAAGGAATGGCGGCGGCCGGAAGGCTGA

Upstream 100 bases:

>100_bases
GCGTGTGCCAAAGCCTTGGGATTGGAGGATGCGGTCGAGGGTCATGGGAAATTGAATCGTCAGTGAAAATGTTTCGGAGG
GCTGGGCCATCCGTATCGCT

Downstream 100 bases:

>100_bases
CTGCCGCGCAGGACGGATACGCGTGCGGGCTTCAGGCAGCCAGCATCGCGCTTTCGCGGTAGTGGCGCTGCGCCTCTTCG
GGCCGCTCGGTCTCTTCGAA

Product: deoxyribodipyrimidine photo-lyase type I

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MLGYRPANLHQGTMQGADTPAHARPPYRIDHQFDRGLVWFRRDLRSTDHAALHYALKHCREVWCVFVFDRDILDPLLARG
LKGDRRVDFIRASLIELDATLRAAGGGLIVMNDSAASAIPALARELGVQAVFANHDYEPDAVARDAAVRDSLAGDARVLF
TFKDQVIFERDEILTSQGKPFAVFTPYKNAWLKTIQPFDLRPYPIEPYLHALAPLPSKRLVPLPSLEDLGFAPSNLAEVA
MPTGSSGGQALFEEFTSRMGDYGRRRDYPALRGPSYLSVHLRFGTVSIRTLARAAHGAVLRGGADSAGAATWLSELVWRD
FYFMILHHHPRVAAGEAFHPAYDIIRWRDGETGDRYFESWRDAATGYPLVDAAMLQIRQSGYMHNRLRMVTASFLVKDLG
VDWRRGEHYFADQLNDFDFAANNGGWQWAASTGCDAQPWFRIFNPVTQSEKFDPQGRFIRKYLPQLAALPDKYVHAPWTA
PQDVLLAAGVRLGENYPRPLVQHDVARKETLERYAVVKEWRRPEG

Sequences:

>Translated_525_residues
MLGYRPANLHQGTMQGADTPAHARPPYRIDHQFDRGLVWFRRDLRSTDHAALHYALKHCREVWCVFVFDRDILDPLLARG
LKGDRRVDFIRASLIELDATLRAAGGGLIVMNDSAASAIPALARELGVQAVFANHDYEPDAVARDAAVRDSLAGDARVLF
TFKDQVIFERDEILTSQGKPFAVFTPYKNAWLKTIQPFDLRPYPIEPYLHALAPLPSKRLVPLPSLEDLGFAPSNLAEVA
MPTGSSGGQALFEEFTSRMGDYGRRRDYPALRGPSYLSVHLRFGTVSIRTLARAAHGAVLRGGADSAGAATWLSELVWRD
FYFMILHHHPRVAAGEAFHPAYDIIRWRDGETGDRYFESWRDAATGYPLVDAAMLQIRQSGYMHNRLRMVTASFLVKDLG
VDWRRGEHYFADQLNDFDFAANNGGWQWAASTGCDAQPWFRIFNPVTQSEKFDPQGRFIRKYLPQLAALPDKYVHAPWTA
PQDVLLAAGVRLGENYPRPLVQHDVARKETLERYAVVKEWRRPEG
>Mature_525_residues
MLGYRPANLHQGTMQGADTPAHARPPYRIDHQFDRGLVWFRRDLRSTDHAALHYALKHCREVWCVFVFDRDILDPLLARG
LKGDRRVDFIRASLIELDATLRAAGGGLIVMNDSAASAIPALARELGVQAVFANHDYEPDAVARDAAVRDSLAGDARVLF
TFKDQVIFERDEILTSQGKPFAVFTPYKNAWLKTIQPFDLRPYPIEPYLHALAPLPSKRLVPLPSLEDLGFAPSNLAEVA
MPTGSSGGQALFEEFTSRMGDYGRRRDYPALRGPSYLSVHLRFGTVSIRTLARAAHGAVLRGGADSAGAATWLSELVWRD
FYFMILHHHPRVAAGEAFHPAYDIIRWRDGETGDRYFESWRDAATGYPLVDAAMLQIRQSGYMHNRLRMVTASFLVKDLG
VDWRRGEHYFADQLNDFDFAANNGGWQWAASTGCDAQPWFRIFNPVTQSEKFDPQGRFIRKYLPQLAALPDKYVHAPWTA
PQDVLLAAGVRLGENYPRPLVQHDVARKETLERYAVVKEWRRPEG

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=500, Percent_Identity=31.4, Blast_Score=207, Evalue=3e-53,
Organism=Homo sapiens, GI4758072, Length=498, Percent_Identity=29.1164658634538, Blast_Score=206, Evalue=4e-53,
Organism=Homo sapiens, GI188536103, Length=449, Percent_Identity=30.5122494432071, Blast_Score=185, Evalue=8e-47,
Organism=Escherichia coli, GI1786926, Length=492, Percent_Identity=39.4308943089431, Blast_Score=301, Evalue=9e-83,
Organism=Saccharomyces cerevisiae, GI6324962, Length=511, Percent_Identity=27.7886497064579, Blast_Score=192, Evalue=8e-50,
Organism=Drosophila melanogaster, GI17137248, Length=519, Percent_Identity=27.7456647398844, Blast_Score=186, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24585455, Length=519, Percent_Identity=27.7456647398844, Blast_Score=186, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24648152, Length=519, Percent_Identity=26.2042389210019, Blast_Score=145, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 59272; Mature: 59272

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGYRPANLHQGTMQGADTPAHARPPYRIDHQFDRGLVWFRRDLRSTDHAALHYALKHCR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
EVWCVFVFDRDILDPLLARGLKGDRRVDFIRASLIELDATLRAAGGGLIVMNDSAASAIP
HHHEEEEECHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHH
ALARELGVQAVFANHDYEPDAVARDAAVRDSLAGDARVLFTFKDQVIFERDEILTSQGKP
HHHHHHCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCC
FAVFTPYKNAWLKTIQPFDLRPYPIEPYLHALAPLPSKRLVPLPSLEDLGFAPSNLAEVA
EEEECCCCHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHCCCCCCCHHHHC
MPTGSSGGQALFEEFTSRMGDYGRRRDYPALRGPSYLSVHLRFGTVSIRTLARAAHGAVL
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHCCCEE
RGGADSAGAATWLSELVWRDFYFMILHHHPRVAAGEAFHPAYDIIRWRDGETGDRYFESW
ECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHEEECCCCCHHHHHHHH
RDAATGYPLVDAAMLQIRQSGYMHNRLRMVTASFLVKDLGVDWRRGEHYFADQLNDFDFA
HHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCEE
ANNGGWQWAASTGCDAQPWFRIFNPVTQSEKFDPQGRFIRKYLPQLAALPDKYVHAPWTA
CCCCCEEEECCCCCCCCHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHCCCCHHEECCCCC
PQDVLLAAGVRLGENYPRPLVQHDVARKETLERYAVVKEWRRPEG
CHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLGYRPANLHQGTMQGADTPAHARPPYRIDHQFDRGLVWFRRDLRSTDHAALHYALKHCR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
EVWCVFVFDRDILDPLLARGLKGDRRVDFIRASLIELDATLRAAGGGLIVMNDSAASAIP
HHHEEEEECHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHH
ALARELGVQAVFANHDYEPDAVARDAAVRDSLAGDARVLFTFKDQVIFERDEILTSQGKP
HHHHHHCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCC
FAVFTPYKNAWLKTIQPFDLRPYPIEPYLHALAPLPSKRLVPLPSLEDLGFAPSNLAEVA
EEEECCCCHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHCCCCCCCHHHHC
MPTGSSGGQALFEEFTSRMGDYGRRRDYPALRGPSYLSVHLRFGTVSIRTLARAAHGAVL
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHCCCEE
RGGADSAGAATWLSELVWRDFYFMILHHHPRVAAGEAFHPAYDIIRWRDGETGDRYFESW
ECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHEEECCCCCHHHHHHHH
RDAATGYPLVDAAMLQIRQSGYMHNRLRMVTASFLVKDLGVDWRRGEHYFADQLNDFDFA
HHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCEE
ANNGGWQWAASTGCDAQPWFRIFNPVTQSEKFDPQGRFIRKYLPQLAALPDKYVHAPWTA
CCCCCEEEECCCCCCCCHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHCCCCHHEECCCCC
PQDVLLAAGVRLGENYPRPLVQHDVARKETLERYAVVKEWRRPEG
CHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]