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The map label for this gene is hemX [H]

Identifier: 73540407

GI number: 73540407

Start: 760155

End: 762224

Strand: Direct

Name: hemX [H]

Synonym: Reut_A0701

Alternate gene names: 73540407

Gene position: 760155-762224 (Clockwise)

Preceding gene: 73540406

Following gene: 73540408

Centisome position: 19.97

GC content: 67.97

Gene sequence:

>2070_bases
ATGCCCCGCCCGACTGTCGTTGTCACACGACCCGCCGGGCAGTCCCGGCAACTGACAGAAGCGCTCCAGGCCGCAGGGCT
CGATGTCCTCGGCTTTCCGCTGCTCTCGATTGGCCCCGCCGCCGATGACGCGCCGCTGCGCGCGGCACTGGCGCGCTTGG
ACCAATTCGCGCTGGTGATTTTTGTCAGCCCCAACGCCATCCAGTACGCGGTCGATGCGCTCGCTGTGGTGCAGGGCATT
GCCGCGGCGCAGTGGCCGCCCGGCGTCCCGGTGGCCGTGGTCGGACCCGCCAGCGTCATGGCGCTGGCCGAACGTGGCAT
TGTCGCGCCCGACTATCGCGTGATCGCGCCGGCGGGTGCCGACGGCAACGGCACGAACGGCGGCGCCAACTGCGAAGAAG
CCCGTTTTGACTCCGAAGCGCTCTGGGCTCAGCTCGAACCGGCCGCACTGCAAGGACGCTCCGTGCTCATCGTCCGCGGC
AACGGTGGCCGCGACTGGCTTGGCGACCGATTGCGCGAAGCGGGCGCGGACGTCACGGCCGTCGAGGCGTACCAGCGCAC
GCTGCCCGAGCCCACTGCCATGCAATGGCAGGCCGTGCGCGACGGCCTGAGCCCTGGCGCCGCCCCGCAAGCCTGGCTGC
TGACCAGCTCCGAAGCCGTGCGCAACCTCGAGACACTGGCGCGACATAACCTGTCGCCGCAGGAACTGGCCACCCTGAAA
CAGGTGCAGTGCATCGCGCCGCATGCGAGAATCGCCGAACAGGCGCAGGCGCTCGGCTTCACGCGCATCCTGCGTGCAGC
GCCAGGCGATGCCGGCCTGCTCGCGGCATGCAAACAGTGGGCGGATGCCTATGCGGGCCCGATAGCCGCAAGTACACCGG
TTCCTGCGGTGGCTCCGGCACCGCGGCCGGAGCCGCTGCCCGTGCCGCCGCCTGCCATGACAAACAGGGTCGAACGCGTG
ACGCAAGAAACTACGTCCTCCTCAACCACCATTCCCCCCGGTGCCACGGCTTCGGCTTCGCCATCGGCCTCGTTCGCCGC
GCCGCGCCGCAATGCCGCATTGTGGGCGCTGGTGGCGGTGCTGGTGCTGGCAACTGCCGGCGGCTTCTGGTGGCTGCAGC
AGCGGGTCGACCACCTGACCGAGGAGCTGGCACGACGCCAGCAGAGCAACGACGCCTTGGTGCAGGAAACGCGCGTGCTG
TCGCGCAACGCGCAGGACACCGTCAAGGAGCTGCAGGCCAAGGTCGGCGCGCTGGACGGCCAGGTCGGCGAGACCCGCGA
CAAGCAGGTCGCGCTCGAACAGGTCTACCAGGACCTGATGCGCAACCGCGATGATTGGGAAATCGCCGAGATCCAGCAGT
TGCTGACCAATGCCGGCCAGCAGCTCCAGCTCACCGGCAACGTCCAGGTCGCACTGGCCGCGCTGCAGAGTGCCGACGCA
CGTCTGGCCCGCACCGACAAGCCGCAGTACAACCTGCTGCGGCGCGCACTGGCGCGCGATATCGCGCGCATGAAGGCGGT
GCCCGATACCGACCTGACCGGCGCTGCCATCAAGCTTGATGAAGCCATCAACCAGATCGACGCGCTGCCGCTGTTGTCCA
GCGAACGCATGCTCGAGCGTACCGAGGCCGAAGACCGCAAGGATGCCGGCCGTGGCGCTGCTGCCAAGGGTACACGCGAG
GCCTCTGCCCCGGCATCGGGCGGCGTAGGCAACTGGTTCGCCCGGCTGTGGAATTACATGCGCGATGAAATGGCGCAGGT
TATCCGCATCCGCAAGGTCGATGACACTGAAGCGCTGTTGCTGTCGGGGGACCAGGGCTGGTTCCTGCGCGAGAACGTCA
AGCTGCGCATGCTCAATGCACGCCTGGCGCTGCTGTCGCGCAATGAGCCGGTCTTCCGCAATGACCTTGCCGCGGCGCAA
GCCATGATCGGCCGTTATTTCGACACGAGGTCAAGGCGCGTGCAAACCGTGCTCACGCTGCTCAAGCAGGCGCAGGCTGG
CGCGGTGACGGTGCAGTTGCCGACCATGGCGGAAAGCCTCGGCGCGCTGCACGCGCTGAAGAAGGAGTAA

Upstream 100 bases:

>100_bases
CGCGTCGGCCGCGCGGGATCTGCTCTCGCAGGGCGCCGCCGAGATTCTCGCCGCGCTGGCCGACACACCGGCTCCCCAAG
ATCCAGCCTGAGGCCGGCCC

Downstream 100 bases:

>100_bases
CCATGCGCCTGCTTTTCTGGGTCGCGATCCTGTTCGGCGGTGCGGTCGGGCTGGCGCTGTTCACGCAGTTCAACCACAGC
AATGTGGTGCTGTTCTATCC

Product: bifunctional uroporphyrinogen-III synthetase/uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Urogen-III methylase; ORF X [H]

Number of amino acids: Translated: 689; Mature: 688

Protein sequence:

>689_residues
MPRPTVVVTRPAGQSRQLTEALQAAGLDVLGFPLLSIGPAADDAPLRAALARLDQFALVIFVSPNAIQYAVDALAVVQGI
AAAQWPPGVPVAVVGPASVMALAERGIVAPDYRVIAPAGADGNGTNGGANCEEARFDSEALWAQLEPAALQGRSVLIVRG
NGGRDWLGDRLREAGADVTAVEAYQRTLPEPTAMQWQAVRDGLSPGAAPQAWLLTSSEAVRNLETLARHNLSPQELATLK
QVQCIAPHARIAEQAQALGFTRILRAAPGDAGLLAACKQWADAYAGPIAASTPVPAVAPAPRPEPLPVPPPAMTNRVERV
TQETTSSSTTIPPGATASASPSASFAAPRRNAALWALVAVLVLATAGGFWWLQQRVDHLTEELARRQQSNDALVQETRVL
SRNAQDTVKELQAKVGALDGQVGETRDKQVALEQVYQDLMRNRDDWEIAEIQQLLTNAGQQLQLTGNVQVALAALQSADA
RLARTDKPQYNLLRRALARDIARMKAVPDTDLTGAAIKLDEAINQIDALPLLSSERMLERTEAEDRKDAGRGAAAKGTRE
ASAPASGGVGNWFARLWNYMRDEMAQVIRIRKVDDTEALLLSGDQGWFLRENVKLRMLNARLALLSRNEPVFRNDLAAAQ
AMIGRYFDTRSRRVQTVLTLLKQAQAGAVTVQLPTMAESLGALHALKKE

Sequences:

>Translated_689_residues
MPRPTVVVTRPAGQSRQLTEALQAAGLDVLGFPLLSIGPAADDAPLRAALARLDQFALVIFVSPNAIQYAVDALAVVQGI
AAAQWPPGVPVAVVGPASVMALAERGIVAPDYRVIAPAGADGNGTNGGANCEEARFDSEALWAQLEPAALQGRSVLIVRG
NGGRDWLGDRLREAGADVTAVEAYQRTLPEPTAMQWQAVRDGLSPGAAPQAWLLTSSEAVRNLETLARHNLSPQELATLK
QVQCIAPHARIAEQAQALGFTRILRAAPGDAGLLAACKQWADAYAGPIAASTPVPAVAPAPRPEPLPVPPPAMTNRVERV
TQETTSSSTTIPPGATASASPSASFAAPRRNAALWALVAVLVLATAGGFWWLQQRVDHLTEELARRQQSNDALVQETRVL
SRNAQDTVKELQAKVGALDGQVGETRDKQVALEQVYQDLMRNRDDWEIAEIQQLLTNAGQQLQLTGNVQVALAALQSADA
RLARTDKPQYNLLRRALARDIARMKAVPDTDLTGAAIKLDEAINQIDALPLLSSERMLERTEAEDRKDAGRGAAAKGTRE
ASAPASGGVGNWFARLWNYMRDEMAQVIRIRKVDDTEALLLSGDQGWFLRENVKLRMLNARLALLSRNEPVFRNDLAAAQ
AMIGRYFDTRSRRVQTVLTLLKQAQAGAVTVQLPTMAESLGALHALKKE
>Mature_688_residues
PRPTVVVTRPAGQSRQLTEALQAAGLDVLGFPLLSIGPAADDAPLRAALARLDQFALVIFVSPNAIQYAVDALAVVQGIA
AAQWPPGVPVAVVGPASVMALAERGIVAPDYRVIAPAGADGNGTNGGANCEEARFDSEALWAQLEPAALQGRSVLIVRGN
GGRDWLGDRLREAGADVTAVEAYQRTLPEPTAMQWQAVRDGLSPGAAPQAWLLTSSEAVRNLETLARHNLSPQELATLKQ
VQCIAPHARIAEQAQALGFTRILRAAPGDAGLLAACKQWADAYAGPIAASTPVPAVAPAPRPEPLPVPPPAMTNRVERVT
QETTSSSTTIPPGATASASPSASFAAPRRNAALWALVAVLVLATAGGFWWLQQRVDHLTEELARRQQSNDALVQETRVLS
RNAQDTVKELQAKVGALDGQVGETRDKQVALEQVYQDLMRNRDDWEIAEIQQLLTNAGQQLQLTGNVQVALAALQSADAR
LARTDKPQYNLLRRALARDIARMKAVPDTDLTGAAIKLDEAINQIDALPLLSSERMLERTEAEDRKDAGRGAAAKGTREA
SAPASGGVGNWFARLWNYMRDEMAQVIRIRKVDDTEALLLSGDQGWFLRENVKLRMLNARLALLSRNEPVFRNDLAAAQA
MIGRYFDTRSRRVQTVLTLLKQAQAGAVTVQLPTMAESLGALHALKKE

Specific function: Siroheme and cobalamin biosynthesis. [C]

COG id: COG2959

COG function: function code H; Uncharacterized enzyme of heme biosynthesis

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790235, Length=241, Percent_Identity=29.4605809128631, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790236, Length=272, Percent_Identity=26.4705882352941, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007470 [H]

Pfam domain/function: PF04375 HemX [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 73874; Mature: 73743

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRPTVVVTRPAGQSRQLTEALQAAGLDVLGFPLLSIGPAADDAPLRAALARLDQFALVI
CCCCCEEEECCCCCCHHHHHHHHHCCCCHHCCHHHHCCCCCCCHHHHHHHHHHCCEEEEE
FVSPNAIQYAVDALAVVQGIAAAQWPPGVPVAVVGPASVMALAERGIVAPDYRVIAPAGA
EECCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHCCCCCCCCEEEEECCC
DGNGTNGGANCEEARFDSEALWAQLEPAALQGRSVLIVRGNGGRDWLGDRLREAGADVTA
CCCCCCCCCCCHHHHCCHHHHHHHCCHHHHCCCEEEEEECCCCCCHHHHHHHHCCCCHHH
VEAYQRTLPEPTAMQWQAVRDGLSPGAAPQAWLLTSSEAVRNLETLARHNLSPQELATLK
HHHHHHHCCCCCHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHHHHCCCCHHHHHHHH
QVQCIAPHARIAEQAQALGFTRILRAAPGDAGLLAACKQWADAYAGPIAASTPVPAVAPA
HHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PRPEPLPVPPPAMTNRVERVTQETTSSSTTIPPGATASASPSASFAAPRRNAALWALVAV
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LVLATAGGFWWLQQRVDHLTEELARRQQSNDALVQETRVLSRNAQDTVKELQAKVGALDG
HHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
QVGETRDKQVALEQVYQDLMRNRDDWEIAEIQQLLTNAGQQLQLTGNVQVALAALQSADA
CCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCH
RLARTDKPQYNLLRRALARDIARMKAVPDTDLTGAAIKLDEAINQIDALPLLSSERMLER
HHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEHHHHHHHHHCCCCCCHHHHHHH
TEAEDRKDAGRGAAAKGTREASAPASGGVGNWFARLWNYMRDEMAQVIRIRKVDDTEALL
HHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
LSGDQGWFLRENVKLRMLNARLALLSRNEPVFRNDLAAAQAMIGRYFDTRSRRVQTVLTL
EECCCCCEEECCCEEEEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKQAQAGAVTVQLPTMAESLGALHALKKE
HHHHCCCEEEEECCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PRPTVVVTRPAGQSRQLTEALQAAGLDVLGFPLLSIGPAADDAPLRAALARLDQFALVI
CCCCEEEECCCCCCHHHHHHHHHCCCCHHCCHHHHCCCCCCCHHHHHHHHHHCCEEEEE
FVSPNAIQYAVDALAVVQGIAAAQWPPGVPVAVVGPASVMALAERGIVAPDYRVIAPAGA
EECCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHCCCCCCCCEEEEECCC
DGNGTNGGANCEEARFDSEALWAQLEPAALQGRSVLIVRGNGGRDWLGDRLREAGADVTA
CCCCCCCCCCCHHHHCCHHHHHHHCCHHHHCCCEEEEEECCCCCCHHHHHHHHCCCCHHH
VEAYQRTLPEPTAMQWQAVRDGLSPGAAPQAWLLTSSEAVRNLETLARHNLSPQELATLK
HHHHHHHCCCCCHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHHHHCCCCHHHHHHHH
QVQCIAPHARIAEQAQALGFTRILRAAPGDAGLLAACKQWADAYAGPIAASTPVPAVAPA
HHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PRPEPLPVPPPAMTNRVERVTQETTSSSTTIPPGATASASPSASFAAPRRNAALWALVAV
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LVLATAGGFWWLQQRVDHLTEELARRQQSNDALVQETRVLSRNAQDTVKELQAKVGALDG
HHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
QVGETRDKQVALEQVYQDLMRNRDDWEIAEIQQLLTNAGQQLQLTGNVQVALAALQSADA
CCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCH
RLARTDKPQYNLLRRALARDIARMKAVPDTDLTGAAIKLDEAINQIDALPLLSSERMLER
HHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEHHHHHHHHHCCCCCCHHHHHHH
TEAEDRKDAGRGAAAKGTREASAPASGGVGNWFARLWNYMRDEMAQVIRIRKVDDTEALL
HHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
LSGDQGWFLRENVKLRMLNARLALLSRNEPVFRNDLAAAQAMIGRYFDTRSRRVQTVLTL
EECCCCCEEECCCEEEEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKQAQAGAVTVQLPTMAESLGALHALKKE
HHHHCCCEEEEECCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3062586; 3054815; 1379743; 9278503; 9298646 [H]