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The map label for this gene is btuF [H]

Identifier: 73540367

GI number: 73540367

Start: 709354

End: 710256

Strand: Reverse

Name: btuF [H]

Synonym: Reut_A0661

Alternate gene names: 73540367

Gene position: 710256-709354 (Counterclockwise)

Preceding gene: 73540368

Following gene: 73540359

Centisome position: 18.66

GC content: 67.55

Gene sequence:

>903_bases
ATGCTCCTCGTCCGCCAGATCGCTGCAGCTGTGCTGCTTGTCGCCACCACTCAGGCCATGGCCACTGTTTCGGCTGTCGA
TGACGCCGGCCAGACCGTCACCCTCGCCCAGCCCGCGCGCCGCGTGGTTTCGCTCGCCCCACACGTGACCGAGCTGCTCT
ACGCCGCGGGCGGCGGCGACCGCATCGTCGGCACCGTGCACTACAGCGACTATCCGCCGCAAGCGCGCGATATTCCGCGC
GTCGGCGACAACAAGGCGCTGGACCTCGAACGGATTGCGGCGCTCAAGCCGGACCTGATCGTGGTGTGGCGCCATGGCAA
TGCGCAGAAGCAGACCGACCGCCTGCGTGCGCTGGGCCTGCCGCTGTTCTACAGCGAGCCGCGCCGGCTCGAGTCGATCC
CGGACAATCTCGAAAAGCTTGGCACGCTGCTCGGCACGGAAGCCACGGCCCATCGCGCCGCGGACGATTTCCGCCAGCGC
GTGGCAGCGCTGCGCAAGACCTACGCGGCCAAGCCGCCGGTCACCGTGTTCTACCAGGTCTGGCAACAGCCGCTGATGAC
GCTGAATGGCCAGCACCTCGTGAGCGACCTGCTTGCACTATGCGGCGGCCGCAATCTGTTCGCGAATGAATCGCCTCTCG
TACCGACCGTGTCGGTAGAGGCCGTGGTGGCCGGCAACCCCGAAGTCATGCTGACCGCAGGCACAGGTGCCACGGTGTCG
GACCGGCCGCTCGCGGACTTCGCGATGTGGGAAAAATGGAAGCAGGTCACGGCGGTCGCACGAGGCAACCTGTTCACGAT
CGACGGCGATCTCGTTACTCGTGCCGGACCGCGCGTGGTACAGGGCGCCGAGCAGATCTGCAAGGACCTCGACACGGCGC
GAAGCCGCCGGCCCGCGCGCTGA

Upstream 100 bases:

>100_bases
GGCCTTCGGCTCGGACTGCCACCCGATTCGCCGCACGCATGGCAACAACTGGAAACCGCGCTGTCCGCCGTCACGCGCGC
CTGAACGCACTCTGACCCGC

Downstream 100 bases:

>100_bases
TCAGCCTGCGCTGCGGTTCCACCACGCGAGCTGGGGCGTGCCGCTGCCTAGGCGGAACAGGCAACTCGCTCCGAAACCCA
GTTCCCACCCGAGCGTCGTC

Product: periplasmic binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MLLVRQIAAAVLLVATTQAMATVSAVDDAGQTVTLAQPARRVVSLAPHVTELLYAAGGGDRIVGTVHYSDYPPQARDIPR
VGDNKALDLERIAALKPDLIVVWRHGNAQKQTDRLRALGLPLFYSEPRRLESIPDNLEKLGTLLGTEATAHRAADDFRQR
VAALRKTYAAKPPVTVFYQVWQQPLMTLNGQHLVSDLLALCGGRNLFANESPLVPTVSVEAVVAGNPEVMLTAGTGATVS
DRPLADFAMWEKWKQVTAVARGNLFTIDGDLVTRAGPRVVQGAEQICKDLDTARSRRPAR

Sequences:

>Translated_300_residues
MLLVRQIAAAVLLVATTQAMATVSAVDDAGQTVTLAQPARRVVSLAPHVTELLYAAGGGDRIVGTVHYSDYPPQARDIPR
VGDNKALDLERIAALKPDLIVVWRHGNAQKQTDRLRALGLPLFYSEPRRLESIPDNLEKLGTLLGTEATAHRAADDFRQR
VAALRKTYAAKPPVTVFYQVWQQPLMTLNGQHLVSDLLALCGGRNLFANESPLVPTVSVEAVVAGNPEVMLTAGTGATVS
DRPLADFAMWEKWKQVTAVARGNLFTIDGDLVTRAGPRVVQGAEQICKDLDTARSRRPAR
>Mature_300_residues
MLLVRQIAAAVLLVATTQAMATVSAVDDAGQTVTLAQPARRVVSLAPHVTELLYAAGGGDRIVGTVHYSDYPPQARDIPR
VGDNKALDLERIAALKPDLIVVWRHGNAQKQTDRLRALGLPLFYSEPRRLESIPDNLEKLGTLLGTEATAHRAADDFRQR
VAALRKTYAAKPPVTVFYQVWQQPLMTLNGQHLVSDLLALCGGRNLFANESPLVPTVSVEAVVAGNPEVMLTAGTGATVS
DRPLADFAMWEKWKQVTAVARGNLFTIDGDLVTRAGPRVVQGAEQICKDLDTARSRRPAR

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=253, Percent_Identity=33.596837944664, Blast_Score=145, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 32511; Mature: 32511

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLVRQIAAAVLLVATTQAMATVSAVDDAGQTVTLAQPARRVVSLAPHVTELLYAAGGGD
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCC
RIVGTVHYSDYPPQARDIPRVGDNKALDLERIAALKPDLIVVWRHGNAQKQTDRLRALGL
EEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCC
PLFYSEPRRLESIPDNLEKLGTLLGTEATAHRAADDFRQRVAALRKTYAAKPPVTVFYQV
CCEECCCHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
WQQPLMTLNGQHLVSDLLALCGGRNLFANESPLVPTVSVEAVVAGNPEVMLTAGTGATVS
HHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCCEEEEECCCCCCC
DRPLADFAMWEKWKQVTAVARGNLFTIDGDLVTRAGPRVVQGAEQICKDLDTARSRRPAR
CCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLLVRQIAAAVLLVATTQAMATVSAVDDAGQTVTLAQPARRVVSLAPHVTELLYAAGGGD
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCC
RIVGTVHYSDYPPQARDIPRVGDNKALDLERIAALKPDLIVVWRHGNAQKQTDRLRALGL
EEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCC
PLFYSEPRRLESIPDNLEKLGTLLGTEATAHRAADDFRQRVAALRKTYAAKPPVTVFYQV
CCEECCCHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
WQQPLMTLNGQHLVSDLLALCGGRNLFANESPLVPTVSVEAVVAGNPEVMLTAGTGATVS
HHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCCEEEEECCCCCCC
DRPLADFAMWEKWKQVTAVARGNLFTIDGDLVTRAGPRVVQGAEQICKDLDTARSRRPAR
CCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA