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The map label for this gene is cobS [H]

Identifier: 73540365

GI number: 73540365

Start: 708002

End: 708778

Strand: Direct

Name: cobS [H]

Synonym: Reut_A0659

Alternate gene names: 73540365

Gene position: 708002-708778 (Clockwise)

Preceding gene: 73540364

Following gene: 73540366

Centisome position: 18.6

GC content: 67.57

Gene sequence:

>777_bases
ATGGCCGACGAACTGCGCTTCTTCCTGACCGCGCTCGGCTACTTCACGCGCGTGCCGCTGCCGTCGCCGCTGGCGCAGTG
GGTTGGCTATGAGCCGCGCTACCTCAATGCGGCGGCGCGCTACTTTCCACTGGTCGGTCTGATGGTCGGCGGCGTGGCCG
TGCTGGTCACGTGGGGCGCGCTGCAGTGGTGGCCGCCGGGCGTCGCGGTGCTGCTCGGCATGGCCGCTACGTTGCTGCTG
ACTGGCGCCTTCCACGAGGACGGGCTTGCCGATTGCGTCGATGCTTTCGGCGGCGCGTGGCGGCGCGAGGACGTGCTGCG
CATCATGCACGACTCGCGCATTGGCGCGTTCGGCGCCATCGCGCTGGTCATCGCGCTGTTGCTCAAGTGGCAACTGCTTG
TCGCCATCGGCGCACGCAGCGGATTGCAGGAGCTGTTGCTCGTGCTGCTCGCCGCGCATGGCGCCAGCCGGGCCATGGCC
GTGAGCTATCTGGCAACGCTGGACTACGTGCGCGACGAGGGTAAGGCCAAGCCCGTGGCGCAGCGGCTTGGCGGGGCGGG
GCTAGGCTTTGCACTGTTGTGCGGTGCTGCGCCGCTGGCGCTGGTGTCGCCACTATTCGCTGGTGTGGCGGTAGTTGCGC
TGGCAATTCTGCGCTTCTTTCTGGGTCGCTATTTCGTGCGCCGCATCGGAGGCTATACGGGCGACTGCCTCGGCATGGCG
CAGCAACTGGCCGAATTGCTTATCTACCTGGTGGCCGCCGCATGGACGTGGTCCTGA

Upstream 100 bases:

>100_bases
GCGCTTGCGTGGCCGCTCGTGTCGTCGGCCGCCGCTTTCCTGAACGAGATGGCCACGTTCAGCGGTGCAGGCGTCAGCGG
CGCCTCGGCCTGACCGCCGC

Downstream 100 bases:

>100_bases
TTCGTCACGCGCGCCCGCAGGTCGGCGGCGGCATTTGCTACGGCAGTCTGGATTTATGTCTGATGACGCCGGTCGAGCCA
TCTCCGGGGGCCATCGTCGC

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MADELRFFLTALGYFTRVPLPSPLAQWVGYEPRYLNAAARYFPLVGLMVGGVAVLVTWGALQWWPPGVAVLLGMAATLLL
TGAFHEDGLADCVDAFGGAWRREDVLRIMHDSRIGAFGAIALVIALLLKWQLLVAIGARSGLQELLLVLLAAHGASRAMA
VSYLATLDYVRDEGKAKPVAQRLGGAGLGFALLCGAAPLALVSPLFAGVAVVALAILRFFLGRYFVRRIGGYTGDCLGMA
QQLAELLIYLVAAAWTWS

Sequences:

>Translated_258_residues
MADELRFFLTALGYFTRVPLPSPLAQWVGYEPRYLNAAARYFPLVGLMVGGVAVLVTWGALQWWPPGVAVLLGMAATLLL
TGAFHEDGLADCVDAFGGAWRREDVLRIMHDSRIGAFGAIALVIALLLKWQLLVAIGARSGLQELLLVLLAAHGASRAMA
VSYLATLDYVRDEGKAKPVAQRLGGAGLGFALLCGAAPLALVSPLFAGVAVVALAILRFFLGRYFVRRIGGYTGDCLGMA
QQLAELLIYLVAAAWTWS
>Mature_257_residues
ADELRFFLTALGYFTRVPLPSPLAQWVGYEPRYLNAAARYFPLVGLMVGGVAVLVTWGALQWWPPGVAVLLGMAATLLLT
GAFHEDGLADCVDAFGGAWRREDVLRIMHDSRIGAFGAIALVIALLLKWQLLVAIGARSGLQELLLVLLAAHGASRAMAV
SYLATLDYVRDEGKAKPVAQRLGGAGLGFALLCGAAPLALVSPLFAGVAVVALAILRFFLGRYFVRRIGGYTGDCLGMAQ
QLAELLIYLVAAAWTWS

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=246, Percent_Identity=32.9268292682927, Blast_Score=108, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 27576; Mature: 27445

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ADELRFFLTALGYFTRVPLPSPLAQWVGYEPRYLNAAARYFPLVGLMVGGVAVLVTWGA
CHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQWWPPGVAVLLGMAATLLLTGAFHEDGLADCVDAFGGAWRREDVLRIMHDSRIGAFGAI
HHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHH
ALVIALLLKWQLLVAIGARSGLQELLLVLLAAHGASRAMAVSYLATLDYVRDEGKAKPVA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHH
QRLGGAGLGFALLCGAAPLALVSPLFAGVAVVALAILRFFLGRYFVRRIGGYTGDCLGMA
HHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
QQLAELLIYLVAAAWTWS
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA