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The map label for this gene is purK [H]

Identifier: 73540260

GI number: 73540260

Start: 599985

End: 601229

Strand: Reverse

Name: purK [H]

Synonym: Reut_A0554

Alternate gene names: 73540260

Gene position: 601229-599985 (Counterclockwise)

Preceding gene: 73540261

Following gene: 73540259

Centisome position: 15.79

GC content: 67.15

Gene sequence:

>1245_bases
ATGCCGACCGATATCCATCCCTACCTCTCCGAAGCCCACACGCCGGAGTCGCGCGCCGAATTTGGCGTCGACAACCCCAG
CGCGCCCATCCTGCCCGGCGCCTGGCTCGGCATGCTCGGCGGCGGTCAGCTTGGCCGCATGTTCACGCATGCGGCGCAGA
GCATGGGCTATCGCGTCTGCGTGCTCGACCCGGACCAGGACAGCCCTGCCGGCTCCGTGGCCGACAAGCATATCTGCGCA
CCCTACACCGATGAGGCCGCGCTCGCCGAGATGGCAAAGCTGTGCCAGGCCGTCAGCACGGAATTCGAAAACGTGCCATC
GCTATCGCTGGACCGGCTCGAACAGCTAGGCGCCTTTGTCGCGCCGCGCGGCTATTGCGTGTCGATTGCGCAGAACCGCA
TCGGCGAGAAGAAGTTCTTTGCCTCGTGCGCCGAGCGTACCGGCGTGCCAACCGCACCGCACTGGGTGATCCAGCACGAT
GCGGATGTCGACCAGTTGCCCGACACGGTGCTGCCCGGCATCCTCAAGACCGCGCGGATGGGCTACGACGGCAAGGGCCA
GGCCCGCGTGAAGACGCGCGAAGACGTGCGCGCGGCATGGAAGGCCATGCAGCACGTGCCATGCGTGCTCGAGCAGATGC
TGCCGCTGGCCTACGAGGTCTCGGTGCTGGCCGCACGCGCCGCCGATGGCAGCACCGCCACCTGGCCGCTGGCCGAGAAC
GTGCATCGCGACGGCATCCTGTTCTCGACGGAGATGCCGTCGGCCAGCGTGTCGGCCGACATCGCCGACCGCGCCCGTGC
CGCCGCCGCGACCATCGCCACGGAAATGGGCTATGTGGGCGTGCTGTGCATCGAATTCTTCGTGCTCACCGACGGCTCGC
TGATCGCCAATGAAATGGCGCCGCGTCCGCACAACTCCGGCCACATCACCATGGACGCGTGCGAGACCAGCCAGTTCGAG
CAACAGGTGCGTGCCATGGCCCGCCTGCCGCTGGGCAGCACGCGCCAGCATTCGGCCGGCAAGATGCTGAACCTGCTTGG
CGATGTATGGTTCGAGTTCGGTTTGGAGCGCACGCCGGCATGGGATGAAGTGCTGGCCCAGCCCGGCGCCAAGCTGCACC
TGTACGGCAAGAGCGACGCGCGCCCGTCGCGCAAGATGGGCCATGTGAACTGCATTGGCGAGCATGCCGAGGCCGCCGCG
CAAGCGTTCGATGTCGCTGCGAAGGCGCTGCACATCCCGCTCTGA

Upstream 100 bases:

>100_bases
CTGGAAGCATTCCGCGCCAGGCAGACCGAAGCCGCGCGCGCGATGACGCTGCCGCTGTAATCCATCGCGCAGTAACCCCC
GGAAACCACGGAACCGAGCA

Downstream 100 bases:

>100_bases
TCCAATCTGACCCTATCCGTCTCGCCCGCTTCAAGGAACACGATGTCGCGCATGCCCACGGCCGCCGAACTGGACGAAGC
CGTCCGCCTGCTGGAGGCCG

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MPTDIHPYLSEAHTPESRAEFGVDNPSAPILPGAWLGMLGGGQLGRMFTHAAQSMGYRVCVLDPDQDSPAGSVADKHICA
PYTDEAALAEMAKLCQAVSTEFENVPSLSLDRLEQLGAFVAPRGYCVSIAQNRIGEKKFFASCAERTGVPTAPHWVIQHD
ADVDQLPDTVLPGILKTARMGYDGKGQARVKTREDVRAAWKAMQHVPCVLEQMLPLAYEVSVLAARAADGSTATWPLAEN
VHRDGILFSTEMPSASVSADIADRARAAAATIATEMGYVGVLCIEFFVLTDGSLIANEMAPRPHNSGHITMDACETSQFE
QQVRAMARLPLGSTRQHSAGKMLNLLGDVWFEFGLERTPAWDEVLAQPGAKLHLYGKSDARPSRKMGHVNCIGEHAEAAA
QAFDVAAKALHIPL

Sequences:

>Translated_414_residues
MPTDIHPYLSEAHTPESRAEFGVDNPSAPILPGAWLGMLGGGQLGRMFTHAAQSMGYRVCVLDPDQDSPAGSVADKHICA
PYTDEAALAEMAKLCQAVSTEFENVPSLSLDRLEQLGAFVAPRGYCVSIAQNRIGEKKFFASCAERTGVPTAPHWVIQHD
ADVDQLPDTVLPGILKTARMGYDGKGQARVKTREDVRAAWKAMQHVPCVLEQMLPLAYEVSVLAARAADGSTATWPLAEN
VHRDGILFSTEMPSASVSADIADRARAAAATIATEMGYVGVLCIEFFVLTDGSLIANEMAPRPHNSGHITMDACETSQFE
QQVRAMARLPLGSTRQHSAGKMLNLLGDVWFEFGLERTPAWDEVLAQPGAKLHLYGKSDARPSRKMGHVNCIGEHAEAAA
QAFDVAAKALHIPL
>Mature_413_residues
PTDIHPYLSEAHTPESRAEFGVDNPSAPILPGAWLGMLGGGQLGRMFTHAAQSMGYRVCVLDPDQDSPAGSVADKHICAP
YTDEAALAEMAKLCQAVSTEFENVPSLSLDRLEQLGAFVAPRGYCVSIAQNRIGEKKFFASCAERTGVPTAPHWVIQHDA
DVDQLPDTVLPGILKTARMGYDGKGQARVKTREDVRAAWKAMQHVPCVLEQMLPLAYEVSVLAARAADGSTATWPLAENV
HRDGILFSTEMPSASVSADIADRARAAAATIATEMGYVGVLCIEFFVLTDGSLIANEMAPRPHNSGHITMDACETSQFEQ
QVRAMARLPLGSTRQHSAGKMLNLLGDVWFEFGLERTPAWDEVLAQPGAKLHLYGKSDARPSRKMGHVNCIGEHAEAAAQ
AFDVAAKALHIPL

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=359, Percent_Identity=33.1476323119777, Blast_Score=151, Evalue=6e-38,
Organism=Escherichia coli, GI1788155, Length=361, Percent_Identity=26.8698060941828, Blast_Score=112, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6324702, Length=391, Percent_Identity=28.9002557544757, Blast_Score=190, Evalue=5e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 44595; Mature: 44464

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTDIHPYLSEAHTPESRAEFGVDNPSAPILPGAWLGMLGGGQLGRMFTHAAQSMGYRVC
CCCCCCHHHHHCCCCHHHHHCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCEEE
VLDPDQDSPAGSVADKHICAPYTDEAALAEMAKLCQAVSTEFENVPSLSLDRLEQLGAFV
EECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
APRGYCVSIAQNRIGEKKFFASCAERTGVPTAPHWVIQHDADVDQLPDTVLPGILKTARM
CCCCCEEHHHHHHCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHCCHHHHHHHHHHHHC
GYDGKGQARVKTREDVRAAWKAMQHVPCVLEQMLPLAYEVSVLAARAADGSTATWPLAEN
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHC
VHRDGILFSTEMPSASVSADIADRARAAAATIATEMGYVGVLCIEFFVLTDGSLIANEMA
CCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCC
PRPHNSGHITMDACETSQFEQQVRAMARLPLGSTRQHSAGKMLNLLGDVWFEFGLERTPA
CCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
WDEVLAQPGAKLHLYGKSDARPSRKMGHVNCIGEHAEAAAQAFDVAAKALHIPL
HHHHHHCCCCEEEEEECCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PTDIHPYLSEAHTPESRAEFGVDNPSAPILPGAWLGMLGGGQLGRMFTHAAQSMGYRVC
CCCCCHHHHHCCCCHHHHHCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCEEE
VLDPDQDSPAGSVADKHICAPYTDEAALAEMAKLCQAVSTEFENVPSLSLDRLEQLGAFV
EECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
APRGYCVSIAQNRIGEKKFFASCAERTGVPTAPHWVIQHDADVDQLPDTVLPGILKTARM
CCCCCEEHHHHHHCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHCCHHHHHHHHHHHHC
GYDGKGQARVKTREDVRAAWKAMQHVPCVLEQMLPLAYEVSVLAARAADGSTATWPLAEN
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHC
VHRDGILFSTEMPSASVSADIADRARAAAATIATEMGYVGVLCIEFFVLTDGSLIANEMA
CCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCC
PRPHNSGHITMDACETSQFEQQVRAMARLPLGSTRQHSAGKMLNLLGDVWFEFGLERTPA
CCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
WDEVLAQPGAKLHLYGKSDARPSRKMGHVNCIGEHAEAAAQAFDVAAKALHIPL
HHHHHHCCCCEEEEEECCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]