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The map label for this gene is dusB [H]

Identifier: 73540195

GI number: 73540195

Start: 531695

End: 532765

Strand: Reverse

Name: dusB [H]

Synonym: Reut_A0489

Alternate gene names: 73540195

Gene position: 532765-531695 (Counterclockwise)

Preceding gene: 73540196

Following gene: 73540194

Centisome position: 14.0

GC content: 64.99

Gene sequence:

>1071_bases
GTGCAGATAGGACCACACCAACTTCGCAACAACCTGTTCGTCGCCCCCATGGCGGGTGTGACGGATCGCCCTTTCCGCCA
GCTGTGCAAGCAGCTCGGGGCAGGCTACGCGGTGTCGGAGATGGTGGCGTCCAACGCACAGCTGTGGAAAAGCGAGAAGA
CCATGCGCCGCGCGAACCATGCCGGCGAGGTCGAACCGATCTCGGTGCAGATCGCCGGCGCGGAGCCAGGCATGATGGCC
GAGGCTGCGCGCTACAACGTCGATCGCGGCGCGCAGATCATCGATATCAATATGGGCTGCCCGGCCAAGAAGGTCTGCAA
TGTGGCCGCGGGGTCCGCGCTGCTGCAGAACGAGCCGCTGGTGGTGCGCATTGTCGAGGCCGTGGTCGCCGCCGTCGGCG
ATCGCGTGCCGGTCACGCTCAAGATCCGCACCGGCTGGAACCGCGAGAACCGCAATGCGCTGCGCATTGCGCGCATGGTG
GAAGACGCGGGCATCAGCATGCTGACCATCCACGGCCGCACGCGCGCGGACTTGTACCACGGCGATGCCGAGTACGAGAC
CATCGCCGCGGTCAAGGCCGAGCTCCGTATCCCGGTGGTAGCCAACGGCGACATCACCACGCCGCAGAAGGCGAAGCAGG
TGCTGGCGCTGACGGGCGCCGACGCCATCATGATCGGCCGCGCGGCGCAAGGCCGGCCTTGGCTGTTCCGTGAGATCGAG
CACTTCATGAAAACCGGCGAACTGCTGCCGTCGCCCGAAGTGGCCGAGATCCGCGCGATCATGAACGCGCACCTCGAAGA
CCACTATGCGTTCTATGGCGAGTTCACCGGCGTGCGCACCGCGCGCAAGCATATCGCGTGGTACACGCGCGGGCTGCGCG
GCGCAAACCTGTTCCGCCACCGCATGAACACGCTGGAAAGCACCGGCGAACAACTGGCCGCGGTCAACGCCTTCTTCGAT
GAGCAGGCGCAATGGTCAGACCGGCTCGTCTATGTCGATGAGGCAGATGCCGGCGGAGAAGAAGCGAACAACAACAACGA
CAACGACAAAGGGGAGTTGCTTGCCGCATGA

Upstream 100 bases:

>100_bases
TTTTTCGTTTCACTCCAGTTACGCCGCCGGGCAATCCCGTTTCGCATAGCGCAGCACGCGCGCCGCCGGGATCAGCGCTG
GCCGGCATCGCCCGCCCAAC

Downstream 100 bases:

>100_bases
GCCGCAACGCTATCGACCAGTGCATCCGGGACAGCCTGGATAGTTACTTTCGGGATCTGGACGGCGAAGAGCCTTCCAAT
ATGTACAACATGGTGCTGGA

Product: dihydrouridine synthase TIM-barrel protein nifR3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MQIGPHQLRNNLFVAPMAGVTDRPFRQLCKQLGAGYAVSEMVASNAQLWKSEKTMRRANHAGEVEPISVQIAGAEPGMMA
EAARYNVDRGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVVRIVEAVVAAVGDRVPVTLKIRTGWNRENRNALRIARMV
EDAGISMLTIHGRTRADLYHGDAEYETIAAVKAELRIPVVANGDITTPQKAKQVLALTGADAIMIGRAAQGRPWLFREIE
HFMKTGELLPSPEVAEIRAIMNAHLEDHYAFYGEFTGVRTARKHIAWYTRGLRGANLFRHRMNTLESTGEQLAAVNAFFD
EQAQWSDRLVYVDEADAGGEEANNNNDNDKGELLAA

Sequences:

>Translated_356_residues
MQIGPHQLRNNLFVAPMAGVTDRPFRQLCKQLGAGYAVSEMVASNAQLWKSEKTMRRANHAGEVEPISVQIAGAEPGMMA
EAARYNVDRGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVVRIVEAVVAAVGDRVPVTLKIRTGWNRENRNALRIARMV
EDAGISMLTIHGRTRADLYHGDAEYETIAAVKAELRIPVVANGDITTPQKAKQVLALTGADAIMIGRAAQGRPWLFREIE
HFMKTGELLPSPEVAEIRAIMNAHLEDHYAFYGEFTGVRTARKHIAWYTRGLRGANLFRHRMNTLESTGEQLAAVNAFFD
EQAQWSDRLVYVDEADAGGEEANNNNDNDKGELLAA
>Mature_356_residues
MQIGPHQLRNNLFVAPMAGVTDRPFRQLCKQLGAGYAVSEMVASNAQLWKSEKTMRRANHAGEVEPISVQIAGAEPGMMA
EAARYNVDRGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVVRIVEAVVAAVGDRVPVTLKIRTGWNRENRNALRIARMV
EDAGISMLTIHGRTRADLYHGDAEYETIAAVKAELRIPVVANGDITTPQKAKQVLALTGADAIMIGRAAQGRPWLFREIE
HFMKTGELLPSPEVAEIRAIMNAHLEDHYAFYGEFTGVRTARKHIAWYTRGLRGANLFRHRMNTLESTGEQLAAVNAFFD
EQAQWSDRLVYVDEADAGGEEANNNNDNDKGELLAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=283, Percent_Identity=31.095406360424, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI239788462, Length=283, Percent_Identity=31.095406360424, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI40807366, Length=230, Percent_Identity=33.0434782608696, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI31742496, Length=228, Percent_Identity=30.2631578947368, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI8923374, Length=180, Percent_Identity=31.6666666666667, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1789660, Length=320, Percent_Identity=57.1875, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1788462, Length=254, Percent_Identity=31.8897637795276, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI145693211, Length=303, Percent_Identity=25.0825082508251, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17543114, Length=243, Percent_Identity=28.8065843621399, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI25144369, Length=226, Percent_Identity=32.3008849557522, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17507177, Length=313, Percent_Identity=28.1150159744409, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17510279, Length=247, Percent_Identity=27.5303643724696, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323433, Length=263, Percent_Identity=28.8973384030418, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323437, Length=228, Percent_Identity=28.0701754385965, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323560, Length=237, Percent_Identity=30.8016877637131, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24585320, Length=240, Percent_Identity=32.5, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24580595, Length=251, Percent_Identity=29.8804780876494, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19920448, Length=251, Percent_Identity=29.8804780876494, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19921524, Length=227, Percent_Identity=28.6343612334802, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI45549423, Length=264, Percent_Identity=28.030303030303, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 39097; Mature: 39097

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIGPHQLRNNLFVAPMAGVTDRPFRQLCKQLGAGYAVSEMVASNAQLWKSEKTMRRANH
CCCCHHHHHCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHCC
AGEVEPISVQIAGAEPGMMAEAARYNVDRGAQIIDINMGCPAKKVCNVAAGSALLQNEPL
CCCCCEEEEEEECCCCCHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCHHHCCCCH
VVRIVEAVVAAVGDRVPVTLKIRTGWNRENRNALRIARMVEDAGISMLTIHGRTRADLYH
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCEEEC
GDAEYETIAAVKAELRIPVVANGDITTPQKAKQVLALTGADAIMIGRAAQGRPWLFREIE
CCCCHHHHHHHHHHEEEEEEECCCCCCHHHHHHEEEECCCCEEEEECCCCCCCHHHHHHH
HFMKTGELLPSPEVAEIRAIMNAHLEDHYAFYGEFTGVRTARKHIAWYTRGLRGANLFRH
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHCCCCHHHHHHH
RMNTLESTGEQLAAVNAFFDEQAQWSDRLVYVDEADAGGEEANNNNDNDKGELLAA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEECC
>Mature Secondary Structure
MQIGPHQLRNNLFVAPMAGVTDRPFRQLCKQLGAGYAVSEMVASNAQLWKSEKTMRRANH
CCCCHHHHHCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHCC
AGEVEPISVQIAGAEPGMMAEAARYNVDRGAQIIDINMGCPAKKVCNVAAGSALLQNEPL
CCCCCEEEEEEECCCCCHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCHHHCCCCH
VVRIVEAVVAAVGDRVPVTLKIRTGWNRENRNALRIARMVEDAGISMLTIHGRTRADLYH
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCEEEC
GDAEYETIAAVKAELRIPVVANGDITTPQKAKQVLALTGADAIMIGRAAQGRPWLFREIE
CCCCHHHHHHHHHHEEEEEEECCCCCCHHHHHHEEEECCCCEEEEECCCCCCCHHHHHHH
HFMKTGELLPSPEVAEIRAIMNAHLEDHYAFYGEFTGVRTARKHIAWYTRGLRGANLFRH
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHCCCCHHHHHHH
RMNTLESTGEQLAAVNAFFDEQAQWSDRLVYVDEADAGGEEANNNNDNDKGELLAA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]