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The map label for this gene is otsB [H]

Identifier: 73540120

GI number: 73540120

Start: 453675

End: 454472

Strand: Direct

Name: otsB [H]

Synonym: Reut_A0414

Alternate gene names: 73540120

Gene position: 453675-454472 (Clockwise)

Preceding gene: 73540117

Following gene: 73540121

Centisome position: 11.92

GC content: 67.67

Gene sequence:

>798_bases
ATGCCCCCTTTACCGCTTATCGAACCCAATACCGCGCTGTTTCTCGACTTCGACGGTACGCTGGCCGATCTCGCCCCGCG
GCCCGAGCTTGTGCAGGTAGAGCCCGAACTGGTCGGGACGCTGCGCAGGCTGTACGACCGTCTGGATGGCGCGCTCGCCA
TCGTATCCGGCCGACCGATCATTGAACTAGACCATTTCCTGCAGCCGCTGTTGCTGCCGGCCGCCGGCGTGCACGGTGCG
GAACTCCGCACCGCCGGCGCGCAGATCGAGATGAAGCCCGCGCCCGGCCTGGAGCCGTTGATCCCGCCGCTTGAAGCACT
TGTGCGCCGGCATCCCGGCCTGCGCCTCGAGCGCAAGTCCGTGGCCGTCGCCATCCACTATCGCGAAGCGCCCGAACTCG
AAGGCCTGGTGCGCGCGGCCGTCACCGACGTGCTGCGCCATGCGAACGGGCTGGAGGGCATGCCCGGCAAGATGGTGGTG
GAGATCAAGCCCGCCGGCATCAACAAGGGCGATGCGATCGCAGCTTTCATGAACATGCCGCCATTCGCTGGACGTGGCCC
GCTCTTTGCGGGCGACGATGTGACTGACGAAGCAGGCTTCGCGGTCGTGCGCAAGCTCGGCGGGGTCGGCGTGCTGGTGG
GCCAGCGCGAGACCATGGCTACGGTCAGCGTCACCGGGCCGGCCGCACTGCGCTGCTGGCTACACCGCTCAGCGCGCGCA
TTCGATGGCACACCGGCCCCGGACGAGCGGCCGGCGGTGTCCGATCCACAACGAGATCTTGAGGGGGATTCGCTGTGA

Upstream 100 bases:

>100_bases
GAGCACTGCCACGCAGGGAATCGCACCAATGTGACGATCTGCGCCGTGTAGGACAGCGCCCGGTAGGCGCCCGCCGGAAT
CACATGTACGGTTTTACGCG

Downstream 100 bases:

>100_bases
CATCCGCAAGCAACCCAAGCAGCCCATCGGAACTGGGCCAGCAGCCGTCCGAAGGCGTGCACAAGACGGTCGACGGCGGC
GCGATTCATGCCACGCCATC

Product: trehalose-phosphatase

Products: NA

Alternate protein names: TPP; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase [H]

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MPPLPLIEPNTALFLDFDGTLADLAPRPELVQVEPELVGTLRRLYDRLDGALAIVSGRPIIELDHFLQPLLLPAAGVHGA
ELRTAGAQIEMKPAPGLEPLIPPLEALVRRHPGLRLERKSVAVAIHYREAPELEGLVRAAVTDVLRHANGLEGMPGKMVV
EIKPAGINKGDAIAAFMNMPPFAGRGPLFAGDDVTDEAGFAVVRKLGGVGVLVGQRETMATVSVTGPAALRCWLHRSARA
FDGTPAPDERPAVSDPQRDLEGDSL

Sequences:

>Translated_265_residues
MPPLPLIEPNTALFLDFDGTLADLAPRPELVQVEPELVGTLRRLYDRLDGALAIVSGRPIIELDHFLQPLLLPAAGVHGA
ELRTAGAQIEMKPAPGLEPLIPPLEALVRRHPGLRLERKSVAVAIHYREAPELEGLVRAAVTDVLRHANGLEGMPGKMVV
EIKPAGINKGDAIAAFMNMPPFAGRGPLFAGDDVTDEAGFAVVRKLGGVGVLVGQRETMATVSVTGPAALRCWLHRSARA
FDGTPAPDERPAVSDPQRDLEGDSL
>Mature_264_residues
PPLPLIEPNTALFLDFDGTLADLAPRPELVQVEPELVGTLRRLYDRLDGALAIVSGRPIIELDHFLQPLLLPAAGVHGAE
LRTAGAQIEMKPAPGLEPLIPPLEALVRRHPGLRLERKSVAVAIHYREAPELEGLVRAAVTDVLRHANGLEGMPGKMVVE
IKPAGINKGDAIAAFMNMPPFAGRGPLFAGDDVTDEAGFAVVRKLGGVGVLVGQRETMATVSVTGPAALRCWLHRSARAF
DGTPAPDERPAVSDPQRDLEGDSL

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=232, Percent_Identity=40.948275862069, Blast_Score=171, Evalue=3e-44,
Organism=Drosophila melanogaster, GI19920676, Length=207, Percent_Identity=28.5024154589372, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI221473368, Length=218, Percent_Identity=26.1467889908257, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20129307, Length=218, Percent_Identity=26.1467889908257, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24582490, Length=218, Percent_Identity=26.1467889908257, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 28251; Mature: 28120

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPLPLIEPNTALFLDFDGTLADLAPRPELVQVEPELVGTLRRLYDRLDGALAIVSGRPI
CCCCCEECCCCEEEEEECCCHHHCCCCCCEEEECHHHHHHHHHHHHHCCCEEEEECCCCE
IELDHFLQPLLLPAAGVHGAELRTAGAQIEMKPAPGLEPLIPPLEALVRRHPGLRLERKS
EEHHHHHHHHHHHCCCCCCCCEECCCCEEEECCCCCCCCCCHHHHHHHHHCCCCEEECCE
VAVAIHYREAPELEGLVRAAVTDVLRHANGLEGMPGKMVVEIKPAGINKGDAIAAFMNMP
EEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCC
PFAGRGPLFAGDDVTDEAGFAVVRKLGGVGVLVGQRETMATVSVTGPAALRCWLHRSARA
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCEEEEEEECCHHHHHHHHHHCCCC
FDGTPAPDERPAVSDPQRDLEGDSL
CCCCCCCCCCCCCCCCHHCCCCCCC
>Mature Secondary Structure 
PPLPLIEPNTALFLDFDGTLADLAPRPELVQVEPELVGTLRRLYDRLDGALAIVSGRPI
CCCCEECCCCEEEEEECCCHHHCCCCCCEEEECHHHHHHHHHHHHHCCCEEEEECCCCE
IELDHFLQPLLLPAAGVHGAELRTAGAQIEMKPAPGLEPLIPPLEALVRRHPGLRLERKS
EEHHHHHHHHHHHCCCCCCCCEECCCCEEEECCCCCCCCCCHHHHHHHHHCCCCEEECCE
VAVAIHYREAPELEGLVRAAVTDVLRHANGLEGMPGKMVVEIKPAGINKGDAIAAFMNMP
EEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCC
PFAGRGPLFAGDDVTDEAGFAVVRKLGGVGVLVGQRETMATVSVTGPAALRCWLHRSARA
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCEEEEEEECCHHHHHHHHHHCCCC
FDGTPAPDERPAVSDPQRDLEGDSL
CCCCCCCCCCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]